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Triton™ X-114 Surfact-Amps™ Detergent Solution (Thermo Scientific™)

Thermo Scientific Triton X-114 Surfact-Amps Detergent Solution is a stabilized 10% (w/v) solution of highly-purified Triton™ X-114 detergent, supplied in glass ampules and packaged under nitrogen inert gas, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-114 Surfact-Amps Detergent Solution:

Triton X-114—a nonionic detergent for use in various routine protein and molecular biology techniques
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable— packaged in glass ampules under inert nitrogen gas

Specifications for Triton X-114 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-114:
• Molecular Weight: 537g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: unknown
• Micelle Molecular Weight: unknown
• Critical Micelle Concentration (CMC): 0.21 mM (0.0113%, w/v)
• Cloud Point: 23°C
• Dialyzable: No

Triton X-114 is most frequently used as a component of cell lysis buffers. In certain conditions, this detergent phase-separates in aqueous solutions, a property that has been used to separate hydrophilic and hydrophobic (membrane) proteins.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

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Octylthioglucoside (OTG) Detergent (Thermo Scientific™)

Thermo Scientific Octylthioglucoside is a low-MW nonionic detergent that is effective for cell lysis and nondenaturing protein solubilization. It is resistant to beta-D-glucoside glucohydrolase degradation.

Features of Octylthioglucoside:

• A low-molecular weight, nonionic detergent
• Effective for a variety of cell lysis and protein solubilization methods
• Unaffected by beta-glucosidase enzyme
• More stable than octyl beta-glucoside (OG)
• Can be removed from solution by dialysis
• Optically clear; low absorbance at 280nm

Properties of n-Octyl-β-D-thioglucopyranoside:
• Alternative Names: Octylthioglucoside, OTG
• Chemical Name: n-Octyl-β-D-thioglucopyranoside
• Molecular Weight: 308.44g
• Detergent Class: Nonionic
• Aggregation Number: Unknown
• Micelle Molecular Weight: Unknown
• Critical Micelle Concentration (CMC): 9 mM (0.2772%, w/v)
• Cloud Point: >100°C
• Dialyzable: Yes

Specifications for Octylthioglucoside (OTG) (Part No. 28351):
• Visual: White powder, free of foreign material.
• Solubility: Solution must be clear, colorless and free of particulate matter.
• Identity: The IR scan must show only peaks characteristic for the structure and functional groups of the compound being tested.

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ZOOM™ CHAPS (Invitrogen™)

The ZOOM® IEF Fractionator offers a fast, reliable method to reduce sample complexity, enrich low abundance proteins, and increase the dynamic range of detection. Solution phase isoelectric focusing with the ZOOM® IEF Fractionator provides reproducible separations in three hours. Fractionated samples are ready for further analysis by two dimensional gel electrophoresis (2DE), one dimensional gel electrophoresis (1DE), or two dimensional liquid chromatography/mass spectrometry (2D LC/MS).

The ZOOM® IEF Fractionator System (Figure 1) consists of the following components:

The ZOOM® IEF Fractionator is built for safety, reliability, and durability. It includes easy-to-assemble sample chambers, in-built buffer chambers, buffer trough, removable electrode assembly, and safety lid.
ZOOM® Disks are pre-cast polyacrylamide gels that eliminate the need for manual preparation and minimize any chance of cross contamination. These immobilized buffered disks are pre-labeled, disposable, and designed for single use, ensuring consistent and reproducible fractionation. They resolve samples into as many as 7 fractions (using 8 disks of specific pH) to six fractions (using six disks of specific pH) from pH 3–12. Simply place each disk between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range.
ZOOM® Reagents are proteomic grade reagents, including ZOOM® 2D Protein Solubilizers urea, thiourea, CHAPS, and ampholytes, that ensure optimal conditions for sample preparation.
The Basic Protein Kit, which includes ZOOM® Discs and ZOOM Strips for IEF of basic proteins, focusing buffers, and solubilizer kits.

Sample Chamber and Fractionator Unit Set-up Procedure
Figures 4–6 illustrate assembly of the sample chambers and fractionator unit. The sample chamber and components fit together easily and offer a leak-proof seal. Simply place disks between the sample chambers in the ZOOM® IEF Fractionator to allow separation in a specific pH range. For example, if you want to fractionate proteins between pH 4.6 and 5.4, flank a sample chamber with a ZOOM® Disk of pH 4.6 and one of pH 5.4.

Downstream Processing by 2D Gel Electrophoresis
Following fractionation, the separated samples can be subjected to further analysis using narrow or broad range ZOOM® Strips on the ZOOM® IPGRunner™ System. Follow with second dimension analysis using neutral pH NuPAGE® ZOOM® Gels.

Brij™-35, 30% Solution (Thermo Scientific™)

This Thermo Scientific™ formulation of Brij™ 35 is a stabilized 30% (w/v) solution of Brij-35 detergent for use in various protein methods. Brij™ 35 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or a surfactant in various HPLC applications.

Properties of Brij 35 Detergent:

• Molecular Weight: 1225g
• Detergent Class: Nonionic
• Aggregation Number: 40
• Micelle Molecular Weight: 49,000g
• Critical Micelle Concentration (CMC): 0.09 mM (0.011%, w/v)
• Cloud Point: >100°C
• Dialyzable: No

Features of this Thermo Scientific detergent solution:

Brij 35—a nonionic detergent for use in various protein methods
Accurate—30% detergent solution in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use

Specifications of this Thermo Scientific Brij 35, 30% Solution:

• Visual: Clear, colorless viscous liquid, free of foreign material.
• Concentration: Concentration: 27.0 to 30.0%

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Octyl-beta-Glucoside Detergent (Thermo Scientific™)

Thermo Scientific Octyl-beta-Glucoside is a nonionic detergent that has been widely used for membrane protein solubilization.

Features of Octyl-beta-Glucoside:

• A low-molecular weight, nonionic detergent
• Effective for membrane protein solubilization
• Less stable and less commonly used than octylthioglucoside (OTG)
• Can be removed from solution by dialysis
• Optically clear; low absorbance at 280nm

Properties of N-Octyl-β-D-glucoside:
• Alternative Names: Octyl-beta-glucoside, OG, Octyl-beta-glucopyranoside, Octyl-beta-D-glucopyranoside
• Chemical Name: n-Octyl-β-D-glucoside
• Molecular Weight: 292.37g
• Detergent Class: Nonionic
• Aggregation Number: 27
• Micelle Molecular Weight: 8000g
• Critical Micelle Concentration (CMC): 23 to 25 mM (0.6716 to 0.7300%, w/v)
• Cloud Point: >100°C
• Dialyzable: Yes

Specifications for Octyl-beta-Glucoside (Part No. 28309, 28310):
• Visual: White, free-flowing powder, free of foreign material.
• Solubility: A 20% (D.I. water) solution must be clear, colorless, with no particulates.
• Absorbance (400nm) of a 20% solution: <0.025
• Absorbance (280nm) of a 20% solution: <0.300
• Specific Rotation (20°C, 1%): -29.0 to -32.0°C
• Identity: The IR scan must show only peaks characteristic for the structure and functional groups of the compound being tested.

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Pluronic™ F-127, low UV absorbance (Invitrogen™)

Pluronic F-127 is a nonionic, surfactant polyol (molecular weight approximately 12,500 daltons) that has been found to facilitate the solubilization of water-insoluble dyes and other materials in physiological media.1 Pluronic F-127 is commonly used to help disperse the acetoxymethyl (AM) esters of our ion indicators as well as our cell tracer dyes such as CFDA-SE.

Digitonin (5%) (Invitrogen™)

5% Digitonin is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

DDM (n-dodecyl β-D-maltoside) (10%) (Invitrogen™)

10% DDM (n-dodecyl -D-maltoside) is a detergent solution used to prepare native protein samples for use with the NativePAGE™ Gel System. Also included in the NativePAGE™ Sample Prep Kit, this ready-to-use detergent solution improves solubility of hydrophobic and membrane proteins during sample preparation. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

Sodium Deoxycholate Detergent (Thermo Scientific™)

Thermo Scientific Sodium Deoxycholate is an ionic detergent that is especially useful for disrupting and dissociating protein interactions.

Features of Sodium Deoxycholate:

• Popular anionic, bile-acid detergent for many laboratory uses
• Effective in disrupting and dissociating many types of protein interaction
• Can be removed from solution by dialysis
• Useful for elution or regeneration of certain kinds of affinity columns
• High-purity compound with low UV absorptivity

Properties of Sodium Deoxycholate:
• Alternative Names: Sodium deoxycholic acid; deoxycholate, sodium salt
• Chemical Name: 3, 12-α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt
• Molecular Weight: 414.6g
• Detergent Class: Ionic (anionic)
• Aggregation Number: 5 (average)
• Micelle Molecular Weight: 2000g (average)
• Critical Micelle Concentration (CMC): 2 to 6 mM (0.083 to 0.249%, w/v)
• Cloud Point: Unknown
• Dialyzable: Yes

Specifications for Sodium Deoxycholate (Part No. 89904, 89905):
• Formula: C24H39O4Na
• Molecular Weight: 414.6g
• Purity (by HPLC): ≥98%
• Absorbance (1% Detergent Solution): 340nm <0.02; 280nm <0.04; 260nm <0.06
• pH (1% Solution): 5 to 9
• Solubility (in water at 20°C): ≥5%

Sodium deoxycholate (deoxycholic acid) is a water-soluble, bile-acid, ionic detergent commonly used in protein methods. It is most frequently used as a component of cell lysis buffers (e.g., RIPA buffer), but also has been used for liposome preparation, isolation of membrane proteins and lipids, preventing non-specific binding in affinity chromatography and a cell culture media supplement.

The effectiveness of a detergent in any application depend upon the detergent's concentration. Too much or too little detergent can often have a deleterious effect. It is recommended that you examine a variety of detergent concentrations in your application. At concentrations above 2 mM, cholate will form micelles having MW ~2000. The small micelle size allows easy removal by dialysis or gel filtration when needed. Note: removal of a detergent from a protein solution may result in protein precipitation and/or aggregation.

Sodium deoxycholate is the detergent recommended for stripping endotoxin (Lipopolysaccharide or LPS) from immobilized Polymyxin B columns. This is the recommended product for use with the Thermo Scientific Detoxi-Gel Endotoxin Removing Gel.

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