Shop All Electrophoresis Reagents & Buffers

TriTrack DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X TriTrack DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights

• Three-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

TAE Buffer (Tris-acetate-EDTA) (50X) Thermo Scientific™

Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Applications

• Electrophoresis of nucleic acids in agarose and polyacrylamide gels
• Used both as a running buffer and as a gel preparation buffer
• Filtered through a 0.22 µm membrane
• Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA

Note
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.

NuPAGE™ Antioxidant Invitrogen™

NuPAGE Antioxidant prevents sample reoxidation and maintains proteins in a reduced state during protein gel electrophoresis and protein transfer. It is added to the running buffer when performing protein gel electrophoresis under reducing conditions. NuPAGE Antioxidant migrates with reduced proteins to maintain reducing conditions and to prevent reoxidation of sensitive amino acids such as methionine and tryptophan.

NuPAGE Antioxidant should be used with samples reduced using NuPAGE Sample Reducing Agent or other reducing agents. NuPAGE Antioxidant is optimized for the neutral pH of NuPAGE gels and is not compatible with other gel chemistries. It may also be added to NuPAGE Transfer Buffer to enhance transfer of proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

Orange DNA Loading Dye (6X) Thermo Scientific™

Thermo Scientific 6X Orange Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains two different dyes (xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X Orange DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights

• Two-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Analysis of small DNA molecules
• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

TEMED Thermo Scientific™

Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

Features of Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) :

• Chemical name: N,N,N',N'-tetramethylethane-1,2-diamine
• Formula: C6H16N2
• CAS number: 110-18-9
• Molecular weight: 116.21
• Purity: ≥99%
• Refractive index: 1.417 to 1.419
• Boiling range: 119 to 121°C
• Clear liquid at room temperature

10X TBE (powder) Invitrogen™

Ambion® 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application. 10 packets are provided, each containing the necessary powder to make 1 liter of 10X TBE buffer upon the addition of H2O. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Specifications:
Appearance: White crystalline powder Molecular Weight: Tris buffer component - 121.14; Boric acid component - 61.83; EDTA component - 372.3

UltraPure™ TAE Buffer, 10X Invitrogen™

UltraPure™ 10X TAE Buffer is a sterile-filtered solution of 400 mM Tris-acetate and 10 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. It is supplied in 1 L plastic bottles or in a 4 L or 10 L stackable Cubitainer® Box. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA at pH 8.3.

Performance and Quality Testing: No DNase activity detected.

NuPAGE™ Transfer Buffer (20X) Invitrogen™

NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

Fluorescent Compatible Sample Buffer (4X, non-reducing) Invitrogen™

Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is optimized for use in fluorescent western blotting as it does not interfere in fluorescent channels, unlike other standard sample buffers. Fluorescent Compatible Sample Buffer is non-reducing but may also be used under denaturing conditions.

Non-reducing—ready-to-use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
Lane marker dyes—visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
Concentrated—4X formulation provides more versatility than traditional 2X buffers
Ideal for fluorescent detection—formulated to prevent auto fluorescence during fluorescent detection

Pierce™ Lane Marker Reducing Sample Buffer Thermo Scientific™

Thermo Scientific Pierce Lane Marker Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a reducing formulation, with a pink dye buffer-front marker. This Sample Buffer contains DTT as the reducing agent, eliminating the strong odor associated with mercaptoethanol-containing buffers.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Non-Reducing Sample Buffer

No-Stain™ Protein Labeling Reagent Invitrogen™

The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any commonly available imager. No-Stain reagent does not require any particular gels or other reagents and is compatible with gel stains and western workflows.

Instant visualization of proteins in gels
Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes. Since lysine is one of the most abundant amino acids, No-Stain reagent enables detection of all proteins in a gel or on a membrane, and the strong signal emission from the covalently bonded reagent provides nanogram-level sensitivity.

Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 µg)
Sensitive—lower limit of detection of 20 ng per band
Specific—forms bonds only with the lysine side chains of proteins. Unbound reagent does not emit, thereby enabling a superior signal-to-noise ratio
Flexible—no need to change your gels to get stain-free convenience. No-Stain reagent provides stain-free convenience with any gel type (precast or pour-your-own gel)

Achieve the gold standard for quantitative western blotting
Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blotting. Total protein normalization is considered the gold standard for quantitative western blotting. Many leading journals have developed guidelines for submitting western blotting research and select quotes from those guidelines are provided below.

• “For quantitative comparisons, appropriate reagents, controls and imaging methods with linear signal ranges should be used” – Nature
• “Record how data were obtained, whether signal intensity was linear with antigen loading, and how protein loading was normalized” – Journal of Biological Chemistry
• “Normalize signal intensity to total protein loading (assessed by staining membranes for total protein) whenever possible” – Journal of Biological Chemistry
• “House-keeping proteins should not be used for normalization without evidence that manipulations do not affect expression” – Journal of Biological Chemistry

An accurate loading control should display a linear relationship between signal intensity and sample load in all experimental conditions. The signal intensity obtained from labeling of total proteins on a membrane with No-Stain reagent ensures a linear relationship between signal intensity and sample load (see figure below) in all experimental conditions. Therefore, the use of No-Stain reagent in quantitative western blot applications enables the use of total protein as an ideal loading control.

Easy-to-use protocol—labeling of proteins within 10 minutes on either nitrocellulose or PVDF membranes
Rapid visualization using a wide-range of imagers with UV or fluorescence light sources
Accurate total protein normalization—the broad linear range for protein detection of 1–80 μg enables detection of No-Stain signal along with that of your target protein to achieve accurate total protein normalization
Sensitive and stable—nanogram level sensitivity with a stable signal that is compatible with downstream immunodetection steps
Superior analysis—housekeeping proteins are susceptible to signal saturation and other biological variations which are not observed when using No-Stain reagent for total protein normalization

Learn more about No-Stain Protein Labeling Reagent ›

NativePAGE™ Running Buffer (20X) Invitrogen™

NativePAGE™ Running Buffer (20X) is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.

SuperSignal™ Western Blot Enhancer Thermo Scientific™

Thermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detection performed without it.

Features of SuperSignal Western Blot Enhancer:

Increase sensitivity—achieve 3- to 10-fold increase in signal intensity and sensitivity
Improve specificity—improves signal-to-noise ratio for poor quality and low affinity antibodies
Better clarity—reduces background for cleaner Western blots
Membrane compatibility—provides effective signal enhancement with PVDF and nitrocellulose membranes
Substrate compatibility—validated for use with chromogenic, chemiluminescent and fluorescent detection methods

When a protein or antigen is difficult to detect because of low abundance or poor immunoreactivity, use of SuperSignal Western Blot Enhancer can significantly reduce background and enhance detection of low-abundance and weakly immunoreactive antigens.

NuPAGE™ MOPS SDS Running Buffer (20X) Invitrogen™

NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

BupH™ Tris-Glycine-SDS Buffer Packs Thermo Scientific™

Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs:

Tris-glycine-SDS buffer—dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for Tris-glycine gel electrophoresis

BupH Dry-Blend Packs of Tris-glycine-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3.

Related Products
Pierce™ 10X Tris-Glycine SDS Buffer
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