Shop All Electrophoresis Reagents & Buffers

MassRuler DNA Loading Dye (6X) (Thermo Scientific™)

Thermo Scientific 6X MassRuler DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains one dye (bromophenol blue) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X MassRuler DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights

• One-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Analysis of large DNA molecules
• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

Rhinohide™ Polyacrylamide Gel Strengthener Concentrate (Invitrogen™)

Rhinohide polyacrylamide gel strengthener improves upon classic polyacrylamide gel technology by making gels much stronger, providing easier handling and resistance to tearing, without adverse side effects. Rhinohide polyacrylamide gel strengthener is highly recommended for low-percentage gels, large-format gels and gels subject to multiple staining and handling steps. Rhinohide polyacrylamide gel strengthener is also available in a kit (R-33410), which includes an acrylamide/bis-acrylamide mixture (37.5:1 ratio).

Gel Loading Buffer II (Denaturing PAGE) (Invitrogen™)

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.

DNA Loading Dye & SDS Solution (6X) (Thermo Scientific™)

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

• 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
• 100 mM EDTA inhibits metal-dependent nucleases

Applications

• Analysis of DNA samples with high amounts of DNA binding proteins
• Kinetic experiments
• DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

TriTrack DNA Loading Dye (6X) (Thermo Scientific™)

Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X TriTrack DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights

• Three-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

Novex™ Tris-Glycine Native Running Buffer (10X) (Invitrogen™)

Novex Tris-Glycine Native Running Buffer (10X) is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels.

Separate native or denatured proteins
Novex Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex Tris-Glycine gels, use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. To separate native proteins use Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

See all available buffers and reagents available for Native-PAGE

Cathode Buffer Container (CBC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Cathode Buffer Container (CBC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The container has two separate compartments: the left provides the cathode buffer for electrophoresis and the right provides for a capillary wash and spent polymer waste ejection functionality between injections. The CBC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the CBC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Novex™ Tris-Glycine Native Sample Buffer (2X) (Invitrogen™)

Novex Tris-Glycine Native Sample Buffer (2X) is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. It has a pH of 8.6 and contains bromophenol blue as a tracking dye.

See all available buffers and reagents available for Native-PAGE

UltraPure™ Agarose (Invitrogen™)

UltraPure™ Agarose is a polysaccharide used for size-based separation of nucleic acids in agarose gel electrophoresis applications. UltraPure™ Agarose is ideal for resolving DNA and RNA fragments from 100 bp to >30 kb. Features of UltraPure™ Agarose:

• Ideal for analysis and recovery of DNA and RNA for routine applications
• Strong gel structure allows for better handling and less breakage
• Can be used in protein electrophoresis applications such as Ouchterlony (antigen-antibody interaction assay) and radial immunodiffusion (RID) (antigen quantitation assay)

Improved packaging
The new environmentally friendly packaging uses 75% less plastic than the original bottles. This means less energy and raw material used in manufacture, and less waste in landfills. Additionally, the easy-pour spout reduces the likelihood of spills and contamination.

Performance and quality testing
The performance of UltraPure™ Agarose is evaluated to satisfy set specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase activity.

Gel Loading Solution (All-Purpose, Native Agarose) (Invitrogen™)

A 10X solution of 40% Sucrose, 0.17% Xylene Cyanol, and 0.17% Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.

TEMED (Thermo Scientific™)

Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

Features of Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) :

• Chemical name: N,N,N',N'-tetramethylethane-1,2-diamine
• Formula: C6H16N2
• CAS number: 110-18-9
• Molecular weight: 116.21
• Purity: ≥99%
• Refractive index: 1.417 to 1.419
• Boiling range: 119 to 121°C
• Clear liquid at room temperature

ULTRAhyb™ Ultrasensitive Hybridization Buffer (Invitrogen™)

Ambion® ULTRAhyb® contains a unique blend of hybridization accelerators and blocking agents that greatly enhance the levels of hybridization so that signals that once took days to visualize are now apparent in hours. It is supplied in one bottle containing 125 mL.

• Increase the sensitivity of any blot hybridization experiment
• Increase random-primed DNA probe sensitivity up to 100x; RNA probe sensitivity 20x
• Use less probe per blot
• Decrease hybridization time to just 2 hours

Increase Sensitivity Up to 100x or Decrease Hybridization to Just 2 Hours
Using standard hybridization buffers, only 1–5% of target molecules on a blot hybridizes to probe, making blots a relatively insensitive method for nucleic acid analysis (Vernier et al. (1996) Anal Biochem 235: 11-19). With ULTRAhyb® Ultrasensitive Hybridization Buffer, the hybridization reaction approaches completion, so that as few as 10,000 molecules can be detected.

For Any Hybridization Protocol
ULTRAhyb® is compatible with RNA and DNA probes labeled isotopically and nonisotopically in northerns, Southerns, and dot/slot blots. It contains 50% formamide and is compatible with positively charged membranes (see Accessory Products). ULTRAhyb® requires 10–100x less nonisotopic DNA probe than standard hybridization buffers, making it possible to perform many more hybridizations with a single labeling reaction.

Accessory Products:
The BrightStar® -Plus Positively Charged Nylon Membranes (SKU# AM10100, AM10102, or AM10104) are recommended for use with ULTRAhyb®. NorthernMax® Wash Buffers (SKU# AM8673 and AM8674) are ideal for use with ULTRAhyb®.ULTRAhyb®-Oligo (SKU# AM8663) is specifically formulated for oligonucleotide probes.

E-Gel™ Sample Loading Buffer, 1X (Invitrogen™)

E-Gel Sample Loading Buffer is supplied as a ready-to-use 1X solution, and is formulated specifically for maximum performance on E-Gel EX gels as well as other E-Gel precast gels.

NativePAGE™ Running Buffer (20X) (Invitrogen™)

NativePAGE™ Running Buffer (20X) is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.