Shop All Electrophoresis Reagents & Buffers

SuperSignal™ Western Blot Enhancer (Thermo Scientific™)

Thermo Scientific SuperSignal Western Blot Enhancer contains a membrane treatment reagent and a primary antibody diluent that increase both signal intensity and sensitivity 3- to 10-fold compared to a detection performed without it.

Features of SuperSignal Western Blot Enhancer:

Increase sensitivity—achieve 3- to 10-fold increase in signal intensity and sensitivity
Improve specificity—improves signal-to-noise ratio for poor quality and low affinity antibodies
Better clarity—reduces background for cleaner Western blots
Membrane compatibility—provides effective signal enhancement with PVDF and nitrocellulose membranes
Substrate compatibility—validated for use with chromogenic, chemiluminescent and fluorescent detection methods

When a protein or antigen is difficult to detect because of low abundance or poor immunoreactivity, use of SuperSignal Western Blot Enhancer can significantly reduce background and enhance detection of low-abundance and weakly immunoreactive antigens.

UltraPure™ Agarose-1000 (Invitrogen™)

UltraPure™ Agarose-1000 is a polysaccharide (see structure) used for size-based separation of nucleic acids in agarose gel electrophoresis. UltraPure™ Agarose 1000 is specifically formulated for the high- resolution separation of small (<1,000 bp) DNA, RNA, and PCR fragments. This standard melting temperature agarose can resolve DNA fragments differing in as little as 10 bp in size. Benefits of using UltraPure™ Agarose 1000:

• Ideal for analysis and recovery of small DNA and RNA fragments
• Strong gel structure allows for better handling
• Improved clarity allows for enhanced visibility of fragments

Improved packaging
The new environmentally friendly packaging uses 75% less plastic than the original bottles (see figure). This means less energy and raw material used in manufacture, and less waste in landfills. Additionally, the easy-pour spout reduces the likelihood of spills and contamination.

Performance and quality testing
The performance of UltraPure™ Agarose 1000 is evaluated to satisfy specifications in appearance, moisture, gel strength, gelling temperature, melting temperature, sulfate content, electroendosmosis, and DNase/RNase assay.

Note: UltraPure™ Agarose 10000 (Cat. No. 16550-100) is a replacement for the item previously sold under Cat. No. 10975-035.

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 (Invitrogen™)

NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kit includes the following buffers:
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

See all available buffers and reagents available for SDS-PAGE

DNA Coating Solution (Thermo Scientific™)

Thermo Scientific DNA Coating Solution is an efficient method for coating DNA on the surface of microplates and microsample tubes.

Features of DNA Coating Solution:

• No more overnight incubations or heating microplates and waiting
• DNA Coating Solution promotes efficient immobilization of DNA onto microplates—hybridization efficiency is related to efficiency of DNA surface immobilization
• Facilitates high-efficiency coating of DNA onto polystryene or polypropylene surfaces
• Target DNA coated onto microplates permits speed and flexibility in probing
• DNA coated onto plastic surfaces is stabilized sufficiently to allow storage for months prior to use.
• DNA Coating Solution autoclaved to inactivate DNase

ZOOM™ Carrier Ampholytes pH 3-10 (Invitrogen™)

The ZOOM® IPGRunner™ System is the first system to offer oil-free, trouble-free 2D electrophoresis in a mini-gel format. First dimension separation, isoelectric focusing (IEF), is complete in less than three hours. The mini-gel design of the system is easy to handle, eliminates mineral oil overlays, and allows processing of up to 12 samples at once for high-throughput usage. The ZOOM® IPGRunner™ System consists of three main components:

• The ZOOM® IPGRunner™ Mini-Cell (Figure 1) includes a high-voltage electrophoresis core and lid assembly that fits a mini-cell chamber identical to the XCell SureLock™ mini-cell. The mini-cell holds two ZOOM® IPGRunner™ Cassettes.
• The ZOOM® IPGRunner™ Cassettes (Figure 2) are provided ready to use and self-contained, eliminating oil overlays for convenient sample rehydration and IEF. Each cassette holds up to six ZOOM® Strips.
• The ZOOM® Strips are 7 cm long and contain a thin layer of polyacrylamide gel that includes a fixed pH gradient. Each ZOOM® Strip is clearly labeled with a unique identifying number, pH range, and orientation marks (Figure 3). ZOOM® Strips are supplied attached to a tri-fold card (Figure 4) for easy access and removal.

Quick and Simple Set-up Procedure
Figure 5 demonstrates how easy and fast it is to load samples and set up the ZOOM® IPGRunner™ System for IEF. Once IEF is complete, the ZOOM® IPGRunner™ Mini-Cell can be used with NuPAGE® and Novex® ZOOM® gels to complete SDS-PAGE analysis in 40 minutes.

TopVision Low Melting Point Agarose (Thermo Scientific™)

Thermo Scientific TopVision Low Melting Point Agarose is used for in-gel enzymatic process experiments and for nucleic acid recovery after electrophoresis.

Highlights

• Optimal concentration between 0.7-2% in all typical buffer systems
• GQ (Genetic Quality) certified, which ensures that nucleic acids recovered from preparative gels can be used for downstream applications (enzymatic reactions etc.)
• Low DNA/RNA binding
• Excellent gel transparency
• DNase and RNase free
• Suitable for RNA analysis

Applications

• Analytical electrophoresis of nucleic acids
• Preparative electrophoresis
• In-gel enzymatic processing experiments
• Preparation of DNA preparative gels in routine molecular biology techniques
• The DNA recovered from agarose gels after electrophoresis can be used in enzymatic process (restriction, ligation, etc.)

Gel-Dry™ Drying Solution (Invitrogen™)

Gel-Dry™ Drying Solution is one component of the DryEase® Mini-Gel Drying System, which is a simple, easy-to-use kit for drying gels evenly using passive evaporation. The kit allows gels to be assembled on a base between two open frames, so that both outer surfaces of the gels are exposed to air. Consequently, drying is even and cracking is minimized. The proprietary Gel-Dry™ Drying Solution modulates the rate of water release to ensure crack-free gels. With the DryEase® System, gels are dried overnight with uniform tension on all points of the gel to eliminate cracking.

Applications: Gels dried with the DryEase® System are suitable for fluorography, densitometry, autoradiography, or permanent gel storage.

ZOOM™ Carrier Ampholytes pH 4-7 (Invitrogen™)

ZOOM® Carrier Ampholytes are small, soluble molecules with both positive and negative charge groups. They sort based on their isoelectric points in an electric field, thereby establishing a pH gradient in solution or contributing to the gradient when used with IPG strips. Carrier ampholytes help stabilize the pH gradient and current in IPG strips and aid in protein solubility, resulting in reproducible IEF resolution.

BupH™ Tris-Glycine Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard transfer buffer for wet or semi-dry electrophoretic protein transfer from gel to blotting membrane.

Features of Thermo Scientific BupH Tris-Glycine Transfer Buffer Packs:

Tris-glycine buffer—dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for gel-to-membrane electrophoretic transfer

BupH Dry-Blend Packs of Tris-glycine buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 400 mL of water and 100 mL methanol, makes 25 mM Tris, 192 mM glycine, pH 8.

Related Products
Pierce™ 10X Tris-Glycine Buffer

Pierce™ Western Blot Signal Enhancer (Thermo Scientific™)

Thermo Scientific Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

Features of Western Blot Signal Enhancer:

Increases protein detection—most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
Improves antibody binding—the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
Works for nearly any protein—signal enhancement has been demonstrated with targets such as IL-6, p53, NFκB, BRCA1 and EGF
Effective with any substrate—enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
Compatible with any membrane—enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
Fast, 15-minute protocol—optimized for a combination of simplicity, speed and signal enhancement for most proteins
Ready-to-use—no formulating or diluting necessary, and the reagents are stable for storage at room temperature

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.

Acrylamide/Bis 19:1, 40% (w/v) solution (Invitrogen™)

Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions.

E-PAGE™ Loading Buffer 1 (Invitrogen™)

The E-PAGE™ High-Throughput (HTP) Protein Electrophoresis System is the only system available for fast, bufferless, high-throughput protein analysis. It consists of E-PAGE™ 96 gels, E-Base™ integrated devices, the E-Holder™ platform, and the free E-Editor™ 2.0 software.

E-PAGE™ 96 Gels
E-PAGE™ 96 gels (Table 1) are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each E-PAGE™ 96 gel contains 96 sample lanes and 8 marker lanes in a patented staggered well format with a 1.6-cm run length (Figure 1). The well openings of the E-PAGE™ 96 cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic devices (Figure 2). E-PAGE™ 96 cassettes are conveniently opened with a butterfly opener to remove the gel for staining or blotting (Figures 3 and 4). In addition, each E-PAGE™ 96 cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

E-Base™ Integrated Devices
E-PAGE™ 96 gels run in the specially designed E-Base™ electrophoresis bases, which combine the base and the power source in one device. Two types of bases are available from Invitrogen:
1. The mother E-Base™ device contains an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-PAGE™ 96 gel
2. The daughter E-Base™ device connects to the mother E-Base™ (Figure 5) and to other daughter E-Base™ devices for the simultaneous electrophoresis of two or more E-PAGE™ 96 gels. The daughter base does not contain an electrical plug and cannot be used without a mother base

Each base has a power/program button and a timer button on the lower right side of the base for controlling your electrophoresis run. The lower left side of each base contains a lighted LED and a digital timer display (00-99) that indicates where your gel is in the running process. Two programs are pre-set in the E-Base™ units: a 14-minute protein run program for E-PAGE™ 96 gels and a 12-minute DNA program for running E-Gel® 96 Gels. For E-Gel® 48 Gels, use the DNA program extended to 20 minutes due to the longer running distance.

Note: The E-Base™ can run either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 systems. The E-PAGE™ 96 gel is not compatible with current models of the E-Gel® 96 bases (mother and daughter bases).

E-Holder™ platform
The E-Holder™ platform is compatible in size with standard automated systems for convenient loading of samples in a hands-free robot environment when the use of the E-Base™ devices is not practical or while in queue for its availability.

E-Editor™ 2.0 Software
Analysis of E-PAGE™ 96 gel results is fast and convenient, for both stained gels and blots, using the Window®-based E-Editor™ 2.0 software. All 96 lanes (and the additional 8 marker lanes) are grouped and aligned into one image, for easy interpretation and comparison. Moreover, when 384-well assays are performed, the E-Editor™ program allows selection of multiple automated loading patterns and grouping of four different E-PAGE™ 96 gel results into one integrated image. This enables an immediate reference back from the gel or blot lane into the coordinates of the originating plate well to provide quick identification of important results.

Novex™ IEF Anode Buffer (50X) (Invitrogen™)

Novex® IEF Anode Buffer (50X) is optimized pre-mixed IEF anode buffer for using with Novex® IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Restore™ Fluorescent Western Blot Stripping Buffer (Thermo Scientific™)

The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Features of Restore Fluorescent Western Blot Stripping Buffer:

Fast—strip blots in only 15 minutes at room temperature
Saves time—no need to run new gels and prepare a new blot
Conserve samples—reprobe the same PVDF membrane for multiple targets
Economical—less expensive than other commercially available stripping buffers
Efficient—effectively strips blots the first time

Restore Fluorescent Western Blot Stripping Buffer enables the reuse of PVDF membranes, simplifying the Western blot optimization process and allowing the same blot to be reprobed with different primary antibodies to detect alternative targets. Restore Fluorescent Western Blot Stripping Buffer is for use with low-fluorescence PVDF membrane only (Part No. 22860).

Fluorescence Western blotting is a powerful method for detecting multiple targets at once. Restore Fluorescent Western Blot Stripping Buffer allows re-probing of PVDF membranes, saving time and cost. This is ideal when samples are limited and optimization or analysis with different primary antibodies is required.

Traditional stripping methods may adversely alter or remove the sample proteins from the PVDF membrane during the stripping process or may be effective for removing only low-affinity antibodies. In contrast, Restore Fluorescent Western Blot Stripping Buffer Stripping efficiency exceeds 90% while reprobing efficiency matches or exceeds other supplier's formulations. Also, Restore Fluorescent Western Blot Stripping Buffer is conveniently stored at room temperature and easy to use. Simply dilute buffer 1:5 in water and incubate your membrane for 5 to 20 minutes at room temperature.

Anode Buffer Container (ABC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Anode Buffer Container (ABC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The ABC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.