Shop All Electrophoresis Reagents & Buffers

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

NuPAGE™ Transfer Buffer (20X) (Invitrogen™)

NuPAGE® Transfer Buffer (20X) is a buffer used to transfer proteins from NuPAGE® Novex® gels to membranes for Western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer.

NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes.

Related link
Learn more about other Invitrogen™ products for your protein transfer needs.

DNA Loading Dye & SDS Solution (6X) (Thermo Scientific™)

Thermo Scientific 6X DNA Loading Dye & SDS Solution is recommended for agarose gel analysis of DNA samples that contain high amounts of DNA binding proteins. The presence of SDS eliminates DNA-protein interactions and prevents gel-shifts. The high concentration of EDTA protects DNA from degradation by metal-dependent nucleases.

Highlights

• 1% SDS eliminates DNA-protein interactions, prevents appearance of additional bands due to annealing of DNA molecules with cohesive ends
• 100 mM EDTA inhibits metal-dependent nucleases

Applications

• Analysis of DNA samples with high amounts of DNA binding proteins
• Kinetic experiments
• DNA agarose gel analysis after DNA restriction digestions, ligation, or dephosphorylation

Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

NuPAGE™ LDS Sample Buffer (4X) (Invitrogen™)

NuPAGE® LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with NuPAGE® Novex® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample Buffer is a highly viscous and concentrated solution—it contains twice the amount of LDS compared to the amount of SDS in Novex® Tris-Glycine SDS Sample Buffer or in Tricine SDS Sample Buffer. NuPAGE® LDS Sample Buffer also contains a higher concentration glycerol.

Agarose (4%) (Gibco™)

Gibco® 4% Agarose is an autoclaved, high purity, low melting point gel designed for plaque assays and the purification of baculovirus. The 4% Agarose Gel melts at 65 – 70°C and remains fluid at 37°C. When diluted with concentrated insect cell culture medium, Gibco® 4% Agarose produces a gel firm enough to immobilize local infections, yet ductile enough to allow for the efficient removal of plaques for clonal expansion.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

Restore™ Western Blot Stripping Buffer, Trial Size (Thermo Scientific™)

Thermo Scientific Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Features of Restore Western Blot Stripping Buffer:

• Saves time—no need to re-run gels and blots
• Saves costly sample—re-probe the membrane using the same target sample
• Effective—formulation is more efficient at stripping antibodies than homemade buffers
• Gentle—does not damage the target antigen during stripping allowing efficient reprobing
• Odor-free—no mercaptans means no acrid odors
• Economical—less expensive than other commercial stripping buffers

Product Details
Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. Restore Stripping Buffer provides clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen allowing blots to be stripped and reprobed with confidence.

Chemiluminescent Western blot detection with reagents such as Thermo Scientific SuperSignal Substrates for horseradish peroxidase is one of the most common and sensitive methods in use today. Because these substrates do not precipitate and bind to membrane surfaces, Western blots detected by chemiluminescence can be stripped with reagents that remove affinity-bound primary and secondary antibodies. To be effective, a stripping buffer must be strong enough to disassociate bound antibodies but gentle enough to leave the transferred target proteins intact on the nitrocellulose or PVDF membrane. Restore Western Blot Stripping Buffer has these characteristics.

By stripping and reprobing, there is no need to waste rare or costly samples by running multiple gels in order to probe for different targets. A single membrane from one gel can be stripped with Restore Western Blot Stripping Buffer to remove the primary antibodies. Stripping the blot takes only 15 to 30 minutes, depending on the affinity of the primary antibody. After stripping, block and reprobe with a new primary antibody. Alternatively, a blot can be stripped and reprobed with adjusted antibody concentrations to optimize conditions after obtaining initially poor results.

Applications
• Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody
• Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time

Protocol Summary
• Wash blot to remove chemiluminescent substrate.
• Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at 37°C (room temperature is sufficient for some antibodies).
• Remove blot and wash in Wash Buffer (TBS-T or PBS-T).
• Test for sufficient removal of antibodies.
• Perform next immunoblot experiment.

Restore™ Fluorescent Western Blot Stripping Buffer (Thermo Scientific™)

The Thermo Scientific Restore Fluorescent Western Blot Stripping Buffer is a gentle and highly effective reagent for quickly removing primary and near-infrared (IR) dye-labeled secondary antibodies from western blots.

Features of Restore Fluorescent Western Blot Stripping Buffer:

Fast—strip blots in only 15 minutes at room temperature
Saves time—no need to run new gels and prepare a new blot
Conserve samples—reprobe the same PVDF membrane for multiple targets
Economical—less expensive than other commercially available stripping buffers
Efficient—effectively strips blots the first time

Restore Fluorescent Western Blot Stripping Buffer enables the reuse of PVDF membranes, simplifying the Western blot optimization process and allowing the same blot to be reprobed with different primary antibodies to detect alternative targets. Restore Fluorescent Western Blot Stripping Buffer is for use with low-fluorescence PVDF membrane only (Part No. 22860).

Fluorescence Western blotting is a powerful method for detecting multiple targets at once. Restore Fluorescent Western Blot Stripping Buffer allows re-probing of PVDF membranes, saving time and cost. This is ideal when samples are limited and optimization or analysis with different primary antibodies is required.

Traditional stripping methods may adversely alter or remove the sample proteins from the PVDF membrane during the stripping process or may be effective for removing only low-affinity antibodies. In contrast, Restore Fluorescent Western Blot Stripping Buffer Stripping efficiency exceeds 90% while reprobing efficiency matches or exceeds other supplier's formulations. Also, Restore Fluorescent Western Blot Stripping Buffer is conveniently stored at room temperature and easy to use. Simply dilute buffer 1:5 in water and incubate your membrane for 5 to 20 minutes at room temperature.

TopVision Low Melting Point Agarose (Thermo Scientific™)

Thermo Scientific TopVision Low Melting Point Agarose is used for in-gel enzymatic process experiments and for nucleic acid recovery after electrophoresis.

Highlights

• Optimal concentration between 0.7-2% in all typical buffer systems
• GQ (Genetic Quality) certified, which ensures that nucleic acids recovered from preparative gels can be used for downstream applications (enzymatic reactions etc.)
• Low DNA/RNA binding
• Excellent gel transparency
• DNase and RNase free
• Suitable for RNA analysis

Applications

• Analytical electrophoresis of nucleic acids
• Preparative electrophoresis
• In-gel enzymatic processing experiments
• Preparation of DNA preparative gels in routine molecular biology techniques
• The DNA recovered from agarose gels after electrophoresis can be used in enzymatic process (restriction, ligation, etc.)

Novex™ TBE-Urea Sample Buffer (2X) (Invitrogen™)

For optimum performance, Novex® TBE-Urea Sample Buffer is recommended for use with Novex® TBE-urea gels. This buffer contains urea and the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, plus the tracking dyes bromophenol blue and xylene cyanol.

About Novex® TBE-urea gels
Denaturing polyacrylamide TBE-urea gels resolve single-stranded DNA oligos or RNA into sharp, distinct bands. Novex® TBE-urea gels are optimized for the analysis and purification of products ranging from 20-800 bases, making them an ideal choice for synthetic oligo analysis and purification, RNase protection assays (RPAs), in vitro transcription studies, and Northern blot analysis. Novex® TBE-urea gels are designed to run on the XCell SureLock™ Mini-Cell.

NuPAGE™ LDS Sample Buffer (4X) (Invitrogen™)

NuPAGE® LDS Sample Buffer (4X) (250 ml) is used to prepare protein samples for denaturing gel electrophoresis with the NuPAGE® gels. Optimal protein sample preparation for polyacrylamide gel electrophoresis (PAGE) requires denaturing and reducing the protein disulfide bonds. NuPAGE® LDS Sample Buffer contains lithium dodecyl sulfate at a pH of 8.4, which allows for maximal activity of the reducing agent.

Note: NuPAGE® LDS Sample Buffer should be brought to room temperature (25°C) prior to use. NuPAGE® LDS Sample Buffer is a highly viscous and concentrated solution. NuPAGE® LDS buffer contains twice the amount of LDS vs. SDS in Novex® Tris-Glycine SDS Sample Buffer or Tricine SDS Sample Buffer. NuPAGE® LDS Sample Buffer also contains a higher concentration glycerol.

For Research Use Only. Not intended for animal or human therapeutic or diagnostic use.

Gel Loading Buffer II (Denaturing PAGE) (Invitrogen™)

A 1–2X solution of 95% Formamide, 18 mM EDTA, and 0.025% SDS, Xylene Cyanol, and Bromophenol Blue. Supplied in one 1.4 mL tube. All Ambion® Gel Loading Solutions are rigorously tested for non-specific endonuclease activity, exonuclease activity, RNase activity, and for functionality. These are the same solutions we use in-house and in our kits.

TriTrack DNA Loading Dye (6X) (Thermo Scientific™)

Thermo Scientific 6X TriTrack DNA Loading Dye is used to prepare DNA markers and samples for loading on agarose or polyacrylamide gels. It contains three different dyes (bromophenol blue, xylene cyanol FF and orange G) for visual tracking of DNA migration during electrophoresis. The presence of glycerol ensures that the DNA in the ladder and sample forms a layer at the bottom of the well. The EDTA included in the solutions binds divalent metal ions and inhibits metal-dependent nucleases.

6X TriTrack DNA Loading Dye is used for conventional DNA electrophoresis.

Highlights

• Three-color tracking of DNA migration during DNA electrophoresis
• No DNA masking during gel exposure to UV light
• EDTA binds divalent metal ions and inhibits metal dependent nucleases

Applications

• Preparation of DNA ladders, markers, and samples for loading on agarose or polyacrylamide gels

Restore™ Western Blot Stripping Buffer (Thermo Scientific™)

Thermo Scientific Restore Western Blot Stripping Buffer safely and effectively removes primary and secondary antibodies from nitrocellulose and PVDF membranes to allow chemiluminescent Western blots to be reprobed.

Features of Restore Western Blot Stripping Buffer:

• Saves time—no need to re-run gels and blots
• Saves costly sample—re-probe the membrane using the same target sample
• Effective—formulation is more efficient at stripping antibodies than homemade buffers
• Gentle—does not damage the target antigen during stripping allowing efficient reprobing
• Odor-free—no mercaptans means no acrid odors
• Economical—less expensive than other commercial stripping buffers

Product Details
Performing gel electrophoresis and duplicate immunoblot assays to test new primary antibodies or antibody concentrations is time-consuming and expensive. Restore Western Blot Stripping Buffer eliminates this waste when detecting immunoblots with chemiluminescent Western blotting substrates. Restore Stripping Buffer provides clean and efficient removal of primary and secondary antibodies from immunoblots without removing or damaging the immobilized antigen allowing blots to be stripped and reprobed with confidence.

Chemiluminescent Western blot detection with reagents such as Thermo Scientific SuperSignal Substrates for horseradish peroxidase is one of the most common and sensitive methods in use today. Because these substrates do not precipitate and bind to membrane surfaces, Western blots detected by chemiluminescence can be stripped with reagents that remove affinity-bound primary and secondary antibodies. To be effective, a stripping buffer must be strong enough to disassociate bound antibodies but gentle enough to leave the transferred target proteins intact on the nitrocellulose or PVDF membrane. Restore Western Blot Stripping Buffer has these characteristics.

By stripping and reprobing, there is no need to waste rare or costly samples by running multiple gels in order to probe for different targets. A single membrane from one gel can be stripped with Restore Western Blot Stripping Buffer to remove the primary antibodies. Stripping the blot takes only 15 to 30 minutes, depending on the affinity of the primary antibody. After stripping, block and reprobe with a new primary antibody. Alternatively, a blot can be stripped and reprobed with adjusted antibody concentrations to optimize conditions after obtaining initially poor results.

Applications
• Reuse a nitrocellulose or PVDF blot to detect a different target with a different primary antibody
• Reprobe a blot to correct or optimize antibody concentrations that were ineffective the first time

Protocol Summary
• Wash blot to remove chemiluminescent substrate.
• Incubate blot in Restore Western Blot Stripping Buffer for 5 to 15 minutes at 37°C (room temperature is sufficient for some antibodies).
• Remove blot and wash in Wash Buffer (TBS-T or PBS-T).
• Test for sufficient removal of antibodies.
• Perform next immunoblot experiment.

TBE (10X), RNase-free (Invitrogen™)

Molecular biology grade, Ambion® 10X TBE solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.