Shop All DNA⁄RNA Gel Running Buffers

NorthernMax™ 10X Running Buffer (Invitrogen™)

MOPS/Sodium Acetate/EDTA for use in denaturing formaldehyde agarose gel systems. Functionally tested and certified RNase-free, one bottle containing 1 L is provided. Ambion® NorthernMax® reagents are the same components included in the NorthernMax® Ultrasensitive Northern Blotting Kits but in larger sizes. These reagents are supplied ready to use and are certified RNase-free. Comprehensive instructions are included.

TBE Buffer (Tris-borate-EDTA) (10X) (Thermo Scientific™)

Thermo Scientific 10X TBE Buffer (Tris-borate-EDTA) is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels, but is not recommended for preparative gels for recovery of nucleic acids. Since borate in TBE buffer is a strong inhibitor for many enzymes, TAE buffer (Tris Acetate-EDTA buffer, 10X powder, sc-296647) is recommended when looking at enzymatic applications for the DNA sample.

Applications

• Electrophoresis of nucleic acids in agarose and polyacrylamide gels
• Used both as a running buffer and as a gel preparation buffer
• Filtered through a 0.22 µm membrane
• Recommended for electrophoresis of RNA and DNA fragments smaller than 1500 bp

Note
Double-stranded linear nucleic acid molecules migrate about 10% slower in TBE buffer than in TAE buffer.

Pierce™ 20X TAE Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Acetate EDTA (pH 8.3) running buffer used for agarose gel electrophoresis of nucleic acids.

Features of 20X TAE Buffer:

TAE buffer—when diluted to 1X with water, yields 0.04M Tris, 0.04M acetate, 1 mM EDTA, pH 8.2 to 8.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Casting and running buffer for agarose gel electrophoresis of nucleic acids

TBE (10X), RNase-free (Invitrogen™)

Molecular biology grade, Ambion® 10X TBE solution is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

UltraPure™ TBE Buffer, 10X (Invitrogen™)

UltraPure™ 10X TBE Buffer is a sterile-filtered solution of 1 M Tris, 0.9 M boric acid, and 0.01 M EDTA used to prepare 1X buffer for polyacrylamide and agarose gel electrophoresis. UltraPure™ 10X TBE Buffer is available in a 1 L plastic bottle or in a 10 L Cubitainer® box.

Performance and quality testing
No DNase, RNase, or protease activity detected.

UltraPure™ TAE Buffer, 10X (Invitrogen™)

UltraPure™ 10X TAE Buffer is a sterile-filtered solution of 400 mM Tris-acetate and 10 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. It is supplied in 1 L plastic bottles or in a 4 L or 10 L stackable Cubitainer® Box. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA at pH 8.3.

Performance and Quality Testing: No DNase activity detected.

UltraPure™ DNA Typing Grade™ 50X TAE Buffer (Invitrogen™)

UltraPure™ DNA Typing Grade® 50X TAE Buffer is a sterile-filtered solution of 2 M Tris-acetate and 50 mM EDTA. TAE Buffer is the most commonly used buffer for agarose DNA electrophoresis. To prepare a 1X solution, mix one volume of UltraPure™ 50X TAE Buffer with 49 volumes of distilled water. A 1X TAE Buffer solution contains 40 mM Tris-acetate and 1 mM EDTA with a pH of 8.3 at 23“C.

Performance and Quality Testing: No DNase activity detected.

TAE (25X) (Invitrogen™)

Ambion® 25X TAE (High Purity Tris-Acetate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application. 10 packets are provided, each containing the necessary powder to make 1 liter of 25X TAE buffer upon the addition of H2O. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Specifications:
Appearance: White crystalline powder Molecular Weight: Tris-Acetate buffer component - 121.14; EDTA component - 372.3

Pierce™ 10X TBE Buffer (Thermo Scientific™)

Thermo Scientific Pierce 10X TBE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-Borate-EDTA (pH 8.3) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids.

Features of Thermo Scientific Pierce 10X TBE Buffer:

TBE buffer—diluted 10-fold in water, the solution yields 0.089M Tris, 0.089M borate, 2 mM EDTA, pH 8.2 to 8.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Agarose and polyacrylamide gel electrophoresis for analysis of nucleic acids

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X TBE Buffer (Tris-borate-EDTA) makes 0.089M Tris, 0.089M borate, 2 mM EDTA, pH 8.2 to 8.4, when diluted to 1X with water.

10X TBE (powder) (Invitrogen™)

Ambion® 10X TBE (Tris-Borate-EDTA Buffer) is thoroughly tested for RNase and DNase contamination, making it ideal for use in sensitive application. 10 packets are provided, each containing the necessary powder to make 1 liter of 10X TBE buffer upon the addition of H2O. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Specifications:
Appearance: White crystalline powder Molecular Weight: Tris buffer component - 121.14; Boric acid component - 61.83; EDTA component - 372.3

TAE (10X), RNase-free (Invitrogen™)

Molecular biology grade, Ambion® 10X TAE is supplied in one bottle containing 1 L. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

TAE Buffer (Tris-acetate-EDTA) (50X) (Thermo Scientific™)

Thermo Scientific 50X TAE Buffer (Tris-acetate-EDTA) is used for electrophoresis of nucleic acids in agarose and polyacrylamide gels. You can use this buffer for both genomic and large supercoiled DNA, and you can also use this as both a running and a gel preparation buffer.

Applications

• Electrophoresis of nucleic acids in agarose and polyacrylamide gels
• Used both as a running buffer and as a gel preparation buffer
• Filtered through a 0.22 µm membrane
• Recommended for resolution of RNA and DNA fragments larger than 1500 bp, for genomic DNA and for large supercoiled DNA

Note
TAE buffer has a relatively low buffering capacity. Therefore, periodic replacement of the buffer during prolonged electrophoresis is recommended.

Novex™ TBE Running Buffer (5X) (Invitrogen™)

Novex® TBE Gels provide the highest resolution possible for analyzing restriction digests and PCR products. DNA fragments ranging from 10 to 3,000 base pairs are clearly resolved into sharp, tight bands. Novex® DNA Retardation gels are prepared with 0.5X TBE as the gel buffer. This is sufficient for good electrophoretic separation, yet low enough to promote DNA-protein interactions. Novex® TBE and DNA Retardation Gels are designed to run on the XCell SureLock™ Mini-Cell.

Formulation: Novex® TBE and DNA Retardation Gels are manufactured with high-purity tris base, boric acid, EDTA, acrylamide, bisacrylamide, TEMED, and APS.

Recommended Buffers: For optimum performance, Novex® TBE Running Buffer and Novex® Hi-Density TBE Sample Buffer are strongly recommended for use with these gels. Novex® Hi-Density TBE Sample Buffer contains the density agent Ficoll™, which yields sharper, straighter bands than conventional density agents, and the tracking dyes Bromophenol Blue and Xylene Cyanol.