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Novex™ IEF Cathode Buffer pH 3-7 (10X) (Invitrogen™)

Novex® IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

Novex™ IEF Sample Buffer pH 3-7 (2X) (Invitrogen™)

Novex® IEF Sample Buffer pH 3-7 (2X) is optimized pre-mixed IEF sample buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Pierce™ SDS-PAGE Sample Prep Kit (Thermo Scientific™)

The Thermo Scientific Pierce SDS-PAGE Sample Prep Kit quickly and easily removes high levels of salts, denaturants, detergents and other buffer components that interfere with polyacrylamide gel electrophoresis of proteins.

Features of the SDS-PAGE Sample Prep Kit:

Eliminates gel artifacts caused by incompatible contaminants—removes dyes, reducing agents, detergents, sugars, glycerol, guanidine, urea and ammonium sulfate to provide reproducible results by SDS-PAGE analysis
Compatible with the BCA protein assay—allows protein quantitation of the processed sample before gel-loading
Enriches dilute protein solutions—concentrates protein samples for SDS-PAGE analysis by six-fold in less than 20 minutes
Powerful and flexible—process 2 to 350 µL of sample containing up to 70 µg of protein; recover 75 to 85% of the total protein in 50 µL of elution buffer
Convenient, complete kit—includes PAGEprep Resin, DMSO, spin cups and sample loading buffer

The SDS-PAGE Sample Prep Kit provides a simple and effective method for concentrating samples while removing chemicals that interfere with SDS-PAGE analysis, such as acids and bases, detergents, guanidine and ammonium sulfate. The proprietary PAGE-prep Resin binds protein in the presence of dimethylsulfoxide (DMSO). Interfering contaminants are then removed by washing the protein-bound resin in an easy-to-use spin cup format. Proteins are eluted in a buffer compatible with the Pierce BCA Protein Assay, allowing quantitation of a portion of the processed sample. The eluted proteins can then be combined with sample buffer and analyzed on the SDS-PAGE system of choice.

Includes:
• PAGEprep resin slurry, DMSO solution, elution buffer, sample buffer, spin cups and collection tubes

Applications:
• Inclusion bodies solubilized in guanidine·HCl
• Samples containing low-pH buffers, thiocyanates or urea
• Proteins precipitated in ammonium sulfate
• Concentration of dilute protein solutions

The SDS-PAGE Sample Prep Kit uses a unique resin composed of modified diatomaceous earth that binds protein in the presence of DMSO. The simple protocol involves combining 2 to 350 µL of sample, containing up to 70 µg of protein, with 20 µL of the PAGEprep Resin in DMSO (supplied). Proteins in the sample bind to the resin in 3 to 5 minutes, after which the nonbound contaminating chemicals are washed away. Finally, the purified proteins are recovered from the resin in 50 µL of Elution Buffer. The recovered protein sample is then ready to mix with the supplied 5X Sample Loading Buffer for gel loading (or another sample loading buffer). The procedure also allows for dilute protein samples to be concentrated up to six-fold, enabling more protein to be loaded per well. In addition, the Thermo Scientific Pierce BCA Protein Assay (Part No. 23225) is compatible with the Elution Buffer and may be used to determine final protein concentration before gel loading.

For reliable SDS-PAGE analysis, protein samples must be prepared in a buffer free of interfering substances and at protein concentrations adequate for analysis. Many interfering substances, which interfere with typical sample buffers for polyacrylamide gel electrophoresis (SDS-PAGE), are difficult to remove by traditional sample preparation methods. For example, protein samples containing 6M guanidine-HCl will precipitate when mixed with Laemmli buffer for SDS-PAGE, causing the sample to run poorly on a gel. Fortunately, samples containing a wide range of interfering chemicals, such as chaotropic agents, detergents, lipids, pH extremes and salts, can be cleaned up within minutes using the SDS-PAGE Sample Prep Kit.

Related Products
Pierce™ SDS-PAGE Sample Prep Kit

Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371 (Invitrogen™)

Novex® pH 3-7 IEF Buffer Kit is optimized for using with Novex® pH 3-7 IEF gels. Each kit includes LC5300, LC5370, LC5371

Anode Buffer Container (ABC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Anode Buffer Container (ABC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The ABC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Cathode Buffer Container (CBC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Cathode Buffer Container (CBC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The container has two separate compartments: the left provides the cathode buffer for electrophoresis and the right provides for a capillary wash and spent polymer waste ejection functionality between injections. The CBC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the CBC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Novex™ Tris-Glycine SDS Running Buffer (10X) (Invitrogen™)

Novex® Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins, in their denatured state, on Novex® Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands, depending upon the gel percentage used.

Separate native or denatured proteins
Novex®Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex® Tris-Glycine gels, use Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer. To separate native proteins on Novex® Tris-Glycine gels, use Novex® Tris-Glycine Native Sample Buffer and Novex® Tris-Glycine Native Running Buffer.

Easy-to-use format
Pre-mixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

Novex™ Tricine SDS Running Buffer (10X) (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.

Pierce™ 20X Tris-HEPES-SDS Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer is a space-saving stock solution used for preparing the Tris-HEPES-SDS running buffer required for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise and Pierce Precast Gels.

Features of Thermo Scientific Pierce 20X Tris-HEPES-SDS Buffer:

Tris-HEPES-SDS buffer—diluted 20-fold in water, the solution yields 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Tris-HEPES-SDS Buffer makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8, when diluted to 1X with water. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
BupH™ Tris-HEPES-SDS Running Buffer

20X Bolt™ MES SDS Running Buffer (Invitrogen™)

Bolt® buffers and sample reducing agents are optimized for use with Bolt® Bis-Tris Plus gels, and are available in a variety of formats. Note: Bolt® gel run times may vary depending upon the type of running buffer used.

About Bolt® Bis-Tris Plus Gels
Bolt® Bis-Tris Plus gels are precast polyacrylamide gels designed for optimal separation of your small- to medium-sized proteins under denaturing conditions. Similar to NuPAGE® Bis-Tris gels, Bolt® Bis-Tris Plus gels are designed to deliver consistent gel performance and provide a neutral pH environment that minimizes protein modifications. Bolt® gels are ideal for western blot transfer and analysis, and any other techniques where protein integrity is crucial. Also use Bolt® gels to obtain optimal results for your day-to-day protein separation needs. Bolt® Bis-Tris Plus gels come in four gel types and multiple well formats. A Mini Gel Tank is required for use of all Bolt® gels.

BupH™ Tris-HEPES-SDS Running Buffer (Thermo Scientific™)

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer

NuPAGE™ Transfer Buffer (20X) (Invitrogen™)

NuPAGE® Transfer Buffer (20X) is a buffer used to transfer proteins from NuPAGE® Novex® gels to membranes for Western blotting. NuPAGE® Transfer Buffer can be used with the Novex® Semi-Dry Blotter for semi-dry transfer or with the XCell II™ Blot Module for wet (tank) transfer.

NuPAGE® Antioxidant may be used with NuPAGE® Transfer Buffer to enhance transfer of reduced proteins to membranes.

Related link
Learn more about other Invitrogen™ products for your protein transfer needs.

Novex™ Tris-Glycine SDS Sample Buffer (2X) (Invitrogen™)

Novex® Tris-Glycine polyacrylamide gel chemistry is based on the Laemmli system (1) with minor modifications for maximum performance in the pre-cast format. These gels do not contain SDS and can therefore be used to accurately separate both native and denatured proteins. Novex® Tris-Glycine Gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Formulation: Novex® Tris-Glycine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. They do not contain SDS.

Recommended Buffers: By choosing the appropriate Novex® pre-mixed buffer, you can create either native, denaturing or reducing running conditions with any Novex® Tris-Glycine Gel.

E-PAGE™ Loading Buffer 1 (Invitrogen™)

The E-PAGE™ High-Throughput (HTP) Protein Electrophoresis System is the only system available for fast, bufferless, high-throughput protein analysis. It consists of E-PAGE™ 96 gels, E-Base™ integrated devices, the E-Holder™ platform, and the free E-Editor™ 2.0 software.

E-PAGE™ 96 Gels
E-PAGE™ 96 gels (Table 1) are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each E-PAGE™ 96 gel contains 96 sample lanes and 8 marker lanes in a patented staggered well format with a 1.6-cm run length (Figure 1). The well openings of the E-PAGE™ 96 cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic devices (Figure 2). E-PAGE™ 96 cassettes are conveniently opened with a butterfly opener to remove the gel for staining or blotting (Figures 3 and 4). In addition, each E-PAGE™ 96 cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

E-Base™ Integrated Devices
E-PAGE™ 96 gels run in the specially designed E-Base™ electrophoresis bases, which combine the base and the power source in one device. Two types of bases are available from Invitrogen:
1. The mother E-Base™ device contains an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-PAGE™ 96 gel
2. The daughter E-Base™ device connects to the mother E-Base™ (Figure 5) and to other daughter E-Base™ devices for the simultaneous electrophoresis of two or more E-PAGE™ 96 gels. The daughter base does not contain an electrical plug and cannot be used without a mother base

Each base has a power/program button and a timer button on the lower right side of the base for controlling your electrophoresis run. The lower left side of each base contains a lighted LED and a digital timer display (00-99) that indicates where your gel is in the running process. Two programs are pre-set in the E-Base™ units: a 14-minute protein run program for E-PAGE™ 96 gels and a 12-minute DNA program for running E-Gel® 96 Gels. For E-Gel® 48 Gels, use the DNA program extended to 20 minutes due to the longer running distance.

Note: The E-Base™ can run either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 systems. The E-PAGE™ 96 gel is not compatible with current models of the E-Gel® 96 bases (mother and daughter bases).

E-Holder™ platform
The E-Holder™ platform is compatible in size with standard automated systems for convenient loading of samples in a hands-free robot environment when the use of the E-Base™ devices is not practical or while in queue for its availability.

E-Editor™ 2.0 Software
Analysis of E-PAGE™ 96 gel results is fast and convenient, for both stained gels and blots, using the Window®-based E-Editor™ 2.0 software. All 96 lanes (and the additional 8 marker lanes) are grouped and aligned into one image, for easy interpretation and comparison. Moreover, when 384-well assays are performed, the E-Editor™ program allows selection of multiple automated loading patterns and grouping of four different E-PAGE™ 96 gel results into one integrated image. This enables an immediate reference back from the gel or blot lane into the coordinates of the originating plate well to provide quick identification of important results.

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex® Tricine Gels are useful for resolving low molecular weight proteins and peptides. In the tricine system developed by Schaegger and von Jagow (1), tricine replaces glycine in the running buffer. This results in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Formulation: Novex® Tricine Gels are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, and highly purified water. Novex® Tricine Gels have a 4% stacking gel and do not contain SDS. The tricine system requires SDS in sample and running buffers for best results.

Recommended Buffers: Use Tricine Running Buffers and Sample Buffers with Novex® Tricine Gels to obtain the benefits of this gel system. Novex® Tris-Glycine transfer buffer can be used for western blotting.