Shop All Protein Gel Buffers

No-Stain™ Protein Labeling Reagent (Invitrogen™)

The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any commonly available imager. No-Stain reagent does not require any particular gels or other reagents and is compatible with gel stains and western workflows.

Instant visualization of proteins in gels
Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes. Since lysine is one of the most abundant amino acids, No-Stain reagent enables detection of all proteins in a gel or on a membrane, and the strong signal emission from the covalently bonded reagent provides nanogram-level sensitivity.

Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 µg)
Sensitive—lower limit of detection of 20 ng per band
Specific—forms bonds only with the lysine side chains of proteins. Unbound reagent does not emit, thereby enabling a superior signal-to-noise ratio
Flexible—no need to change your gels to get stain-free convenience. No-Stain reagent provides stain-free convenience with any gel type (precast or pour-your-own gel)

Achieve the gold standard for quantitative western blotting
Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blotting. Total protein normalization is considered the gold standard for quantitative western blotting. Many leading journals have developed guidelines for submitting western blotting research and select quotes from those guidelines are provided below.

• “For quantitative comparisons, appropriate reagents, controls and imaging methods with linear signal ranges should be used” – Nature
• “Record how data were obtained, whether signal intensity was linear with antigen loading, and how protein loading was normalized” – Journal of Biological Chemistry
• “Normalize signal intensity to total protein loading (assessed by staining membranes for total protein) whenever possible” – Journal of Biological Chemistry
• “House-keeping proteins should not be used for normalization without evidence that manipulations do not affect expression” – Journal of Biological Chemistry

An accurate loading control should display a linear relationship between signal intensity and sample load in all experimental conditions. The signal intensity obtained from labeling of total proteins on a membrane with No-Stain reagent ensures a linear relationship between signal intensity and sample load (see figure below) in all experimental conditions. Therefore, the use of No-Stain reagent in quantitative western blot applications enables the use of total protein as an ideal loading control.

Easy-to-use protocol—labeling of proteins within 10 minutes on either nitrocellulose or PVDF membranes
Rapid visualization using a wide-range of imagers with UV or fluorescence light sources
Accurate total protein normalization—the broad linear range for protein detection of 1–80 μg enables detection of No-Stain signal along with that of your target protein to achieve accurate total protein normalization
Sensitive and stable—nanogram level sensitivity with a stable signal that is compatible with downstream immunodetection steps
Superior analysis—housekeeping proteins are susceptible to signal saturation and other biological variations which are not observed when using No-Stain reagent for total protein normalization

BupH™ Tris-HEPES-SDS Running Buffer (Thermo Scientific™)

Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of running buffer for gel electrophoresis (SDS-PAGE) with Thermo Scientific Precise Precast Protein Gels.

Features of Thermo Scientific BupH Tris-HEPES-SDS Buffer Packs:

Tris-HEPES-SDS buffer—dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for SDS-PAGE with Thermo Scientific Precise Precast Gels (e.g., Part No. 25224)

BupH Dry-Blend Packs of Tris-HEPES-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M Tris, 0.1M HEPES, 3 mM SDS, pH 8. HEPES is 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid.

Related Products
Pierce™ 20X Tris-HEPES-SDS Buffer

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

NativePAGE™ Running Buffer Kit (Invitrogen™)

The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. Novex Tricine SDS Sample Buffer is specifically formulated for optimal electrophoresis of small proteins and peptides. The sample buffer is formulated with Coomassie Blue G and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie Blue G gives a sharp dye front with Tricine SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.

Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.

See all buffers and reagents for SDS-PAGE ›

20X Bolt™ MES SDS Running Buffer (Invitrogen™)

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

Novex™ IEF Cathode Buffer pH 3-7 (10X) (Invitrogen™)

Novex® IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

20X Bolt™ MOPS SDS Running Buffer (Invitrogen™)

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 (Invitrogen™)

NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kit includes the following buffers:
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

Pierce™ 10X Tris-Glycine Buffer (Thermo Scientific™)

Thermo Scientific Pierce 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for Western blotting.

Features of Thermo Scientific Pierce 10X Tris-Glycine Buffer:

Tris-glycine buffer—diluted 10-fold in water or 20% methanol, the solution yields 0.025M Tris, 0.192M glycine, pH 8.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Required:
• Methanol or ethanol as required by specific protocols.

Applications:
• Transfer buffer for Tris-glycine gel electrophoresis

Related Products
BupH™ Tris-Glycine Buffer Packs

Pierce™ Lane Marker Reducing Sample Buffer (Thermo Scientific™)

Thermo Scientific Pierce Lane Marker Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a reducing formulation, with a pink dye buffer-front marker. This Sample Buffer contains DTT as the reducing agent, eliminating the strong odor associated with mercaptoethanol-containing buffers.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Non-Reducing Sample Buffer

NativePAGE™ Running Buffer (20X) (Invitrogen™)

NativePAGE™ Running Buffer (20X) is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.

NativePAGE™ Sample Prep Kit (Invitrogen™)

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.

Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 (Invitrogen™)

The Novex Tricine SDS Buffer Kit is designed for separation of small- to medium-size proteins on tricine gels. This kit includes the following buffers:
• Tricine SDS Sample Buffer (Cat. No. LC1676)
• Novex Tricine SDS Running Buffer (Cat. No. LC1675)

See all buffers and reagents for SDS-PAGE ›