Shop All Protein Gel Buffers

Novex™ IEF Cathode Buffer pH 3-7 (10X) Invitrogen™

Novex® IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

Novex™ IEF Sample Buffer pH 3-7 (2X) Invitrogen™

Novex® IEF Sample Buffer pH 3-7 (2X) is optimized pre-mixed IEF sample buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371 Invitrogen™

Novex® pH 3-7 IEF Buffer Kit is optimized for using with Novex® pH 3-7 IEF gels. Each kit includes LC5300, LC5370, LC5371

NativePAGE™ Sample Buffer (4X) Invitrogen™

NativePAGE™ Sample Buffer (4X) is a solution used to solubilize native protein samples prior to use with the NativePAGE™ Gel System. The buffer is used along with 10% DDM or 5% Digitonin detergents to solubilize hydrophobic or membrane proteins. This buffer is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

E-PAGE™ Loading Buffer 1 Invitrogen™

The E-PAGE™ High-Throughput (HTP) Protein Electrophoresis System is the only system available for fast, bufferless, high-throughput protein analysis. It consists of E-PAGE™ 96 gels, E-Base™ integrated devices, the E-Holder™ platform, and the free E-Editor™ 2.0 software.

E-PAGE™ 96 Gels
E-PAGE™ 96 gels (Table 1) are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each E-PAGE™ 96 gel contains 96 sample lanes and 8 marker lanes in a patented staggered well format with a 1.6-cm run length (Figure 1). The well openings of the E-PAGE™ 96 cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic devices (Figure 2). E-PAGE™ 96 cassettes are conveniently opened with a butterfly opener to remove the gel for staining or blotting (Figures 3 and 4). In addition, each E-PAGE™ 96 cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

E-Base™ Integrated Devices
E-PAGE™ 96 gels run in the specially designed E-Base™ electrophoresis bases, which combine the base and the power source in one device. Two types of bases are available from Invitrogen:
1. The mother E-Base™ device contains an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-PAGE™ 96 gel
2. The daughter E-Base™ device connects to the mother E-Base™ (Figure 5) and to other daughter E-Base™ devices for the simultaneous electrophoresis of two or more E-PAGE™ 96 gels. The daughter base does not contain an electrical plug and cannot be used without a mother base

Each base has a power/program button and a timer button on the lower right side of the base for controlling your electrophoresis run. The lower left side of each base contains a lighted LED and a digital timer display (00-99) that indicates where your gel is in the running process. Two programs are pre-set in the E-Base™ units: a 14-minute protein run program for E-PAGE™ 96 gels and a 12-minute DNA program for running E-Gel® 96 Gels. For E-Gel® 48 Gels, use the DNA program extended to 20 minutes due to the longer running distance.

Note: The E-Base™ can run either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 systems. The E-PAGE™ 96 gel is not compatible with current models of the E-Gel® 96 bases (mother and daughter bases).

E-Holder™ platform
The E-Holder™ platform is compatible in size with standard automated systems for convenient loading of samples in a hands-free robot environment when the use of the E-Base™ devices is not practical or while in queue for its availability.

E-Editor™ 2.0 Software
Analysis of E-PAGE™ 96 gel results is fast and convenient, for both stained gels and blots, using the Window®-based E-Editor™ 2.0 software. All 96 lanes (and the additional 8 marker lanes) are grouped and aligned into one image, for easy interpretation and comparison. Moreover, when 384-well assays are performed, the E-Editor™ program allows selection of multiple automated loading patterns and grouping of four different E-PAGE™ 96 gel results into one integrated image. This enables an immediate reference back from the gel or blot lane into the coordinates of the originating plate well to provide quick identification of important results.

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) Invitrogen™

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

NativePAGE™ Sample Prep Kit Invitrogen™

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.

NativePAGE™ 5% G-250 Sample Additive Invitrogen™

NativePAGE™ 5% G-250 Sample Additive is added to samples prepared with the NativePAGE™ Sample Buffer and detergents (10% DMM or 5% Digitonin) just prior to applying the samples to NativePAGE™ gels. This Additive is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

BupH™ Tris-Glycine-SDS Buffer Packs Thermo Scientific™

Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs are pouches of dry-blend powder that are each sufficient to make 500 mL of standard (pH 8.3) running buffer for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of Thermo Scientific BupH Tris-Glycine-SDS Buffer Packs:

Tris-glycine-SDS buffer—dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

Applications:
• Running buffer for Tris-glycine gel electrophoresis

BupH Dry-Blend Packs of Tris-glycine-SDS buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 25 mM Tris, 192 mM glycine, 0.1% SDS, pH 8.3.

Related Products
Pierce™ 10X Tris-Glycine SDS Buffer

Novex™ IEF Cathode Buffer pH 3-10 (10X) Invitrogen™

Novex® IEF Cathode Buffer pH 3-10 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

Novex™ Tricine SDS Running Buffer (10X) Invitrogen™

Novex Tricine SDS Running Buffer (2X) is formulated for separation of proteins in their denatured state on tricine gels. Tricine gels are specifically designed for the resolution of low molecular weight proteins. In this buffer system, tricine substitutes glycine in the running buffer, resulting in more efficient stacking and destacking of low molecular weight proteins and higher resolution of smaller peptides.

Recommended buffers: Use with tricine sample buffer to obtain optimal separation with tricine SDS running buffer.

See all buffers and reagents for SDS-PAGE ›

NuPAGE™ LDS Sample Buffer (4X) Invitrogen™

NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol.

Fluorescent Compatible Sample Buffer (4X, non-reducing) Invitrogen™

Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is optimized for use in fluorescent western blotting as it does not interfere in fluorescent channels, unlike other standard sample buffers. Fluorescent Compatible Sample Buffer is non-reducing but may also be used under denaturing conditions.

Non-reducing—ready-to-use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
Lane marker dyes—visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
Concentrated—4X formulation provides more versatility than traditional 2X buffers
Ideal for fluorescent detection—formulated to prevent auto fluorescence during fluorescent detection

Novex™ Tris-Glycine Native Running Buffer (10X) Invitrogen™

Novex Tris-Glycine Native Running Buffer (10X) is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels.

Separate native or denatured proteins
Novex Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex Tris-Glycine gels, use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. To separate native proteins use Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

See all available buffers and reagents available for Native-PAGE

20X Bolt™ MES SDS Running Buffer Invitrogen™

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

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