Shop All Protein Gel Buffers

NativePAGE™ Sample Prep Kit (Invitrogen™)

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.

Novex™ Tris-Glycine SDS Buffer Kit (Invitrogen™)

Pre-mixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

No-Stain™ Protein Labeling Reagent (Invitrogen™)

The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any commonly available imager. No-Stain reagent does not require any particular gels or other reagents and is compatible with gel stains and western workflows.

Instant visualization of proteins in gels
Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes. Since lysine is one of the most abundant amino acids, No-Stain reagent enables detection of all proteins in a gel or on a membrane, and the strong signal emission from the covalently bonded reagent provides nanogram-level sensitivity.

Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 µg)
Sensitive—lower limit of detection of 20 ng per band
Specific—forms bonds only with the lysine side chains of proteins. Unbound reagent does not emit, thereby enabling a superior signal-to-noise ratio
Flexible—no need to change your gels to get stain-free convenience. No-Stain reagent provides stain-free convenience with any gel type (precast or pour-your-own gel)

Achieve the gold standard for quantitative western blotting
Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blotting. Total protein normalization is considered the gold standard for quantitative western blotting. Many leading journals have developed guidelines for submitting western blotting research and select quotes from those guidelines are provided below.

• “For quantitative comparisons, appropriate reagents, controls and imaging methods with linear signal ranges should be used” – Nature
• “Record how data were obtained, whether signal intensity was linear with antigen loading, and how protein loading was normalized” – Journal of Biological Chemistry
• “Normalize signal intensity to total protein loading (assessed by staining membranes for total protein) whenever possible” – Journal of Biological Chemistry
• “House-keeping proteins should not be used for normalization without evidence that manipulations do not affect expression” – Journal of Biological Chemistry

An accurate loading control should display a linear relationship between signal intensity and sample load in all experimental conditions. The signal intensity obtained from labeling of total proteins on a membrane with No-Stain reagent ensures a linear relationship between signal intensity and sample load (see figure below) in all experimental conditions. Therefore, the use of No-Stain reagent in quantitative western blot applications enables the use of total protein as an ideal loading control.

Easy-to-use protocol—labeling of proteins within 10 minutes on either nitrocellulose or PVDF membranes
Rapid visualization using a wide-range of imagers with UV or fluorescence light sources
Accurate total protein normalization—the broad linear range for protein detection of 1–80 μg enables detection of No-Stain signal along with that of your target protein to achieve accurate total protein normalization
Sensitive and stable—nanogram level sensitivity with a stable signal that is compatible with downstream immunodetection steps
Superior analysis—housekeeping proteins are susceptible to signal saturation and other biological variations which are not observed when using No-Stain reagent for total protein normalization

Learn more about No-Stain Protein Labeling Reagent ›

20X Bolt™ MES SDS Running Buffer (Invitrogen™)

Bolt MES SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

Novex™ IEF Anode Buffer (50X) (Invitrogen™)

Novex® IEF Anode Buffer (50X) is optimized pre-mixed IEF anode buffer for using with Novex® IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Pierce™ LDS Sample Buffer, Non-Reducing (4X) (Thermo Scientific™)

Thermo Scientific Pierce LDS Sample Loading Buffer (4X) is a nonreducing lithium dodecyl sulfate sample loading buffer, a unique alternative to homemade and other commercial gel-loading dyes.

Features of LDS Sample Loading Buffer:

Concentrated—4X formulation provides more versatility than traditional 2X buffers
Nonreducing—ready to use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
SDS alternative—lithium dodecyl sulfate (LDS) replaces sodium dodecyl sulfate (SDS) of traditional loading buffers but functions equally well
Physiologic pH—buffered with triethanolamine (pH 7.6) rather than Tris (pH 6.8)
Lane marker dyes—includes trace amounts of phenol red and coomassie G250 as visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed

LDS Sample Buffer, Non-Reducing (4X) is a convenient sample buffer for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). The buffer contains coomassie dye, enabling visualization of the electrophoretic progress by the location of the dye front. The LDS Sample Buffer, Non-Reducing (4X) may be used in denaturing gels and is compatible with both coomassie and silver staining and Western blotting applications.

Novex™ Tris-Glycine Transfer Buffer (25X) (Invitrogen™)

Novex Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis using Tris-Glycine gels.

To use: This buffer should be diluted to a 1X solution with a water/methanol mixture to yield a final methanol concentration of 20% for optimal results.

See all available buffers and reagents available for SDS-PAGE

Pierce™ 10X Tris-Glycine SDS Buffer (Thermo Scientific™)

Thermo Scientific Pierce 10X Tris-Glycine-SDS Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine-SDS (pH 8.3) running buffer used for SDS-PAGE (protein polyacrylamide gel electrophoresis).

Features of 10X Tris-Glycine-SDS Buffer:

Tris-glycine-SDS buffer—diluted 10-fold in water, the solution yields 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Tris-Glycine-SDS Buffer makes 0.025M Tris, 0.192M glycine, 0.1% SDS, pH 8.5, when diluted to 1X with water. SDS is sodium dodecyl sulfate.

Applications:
• Running buffer for Tris-glycine gel electrophoresis

Related Products
BupH™ Tris-Glycine-SDS Buffer Packs

Anode Buffer Container (ABC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Anode Buffer Container (ABC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The ABC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371 (Invitrogen™)

Novex® pH 3-7 IEF Buffer Kit is optimized for using with Novex® pH 3-7 IEF gels. Each kit includes LC5300, LC5370, LC5371

NuPAGE™ MES SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MES SDS Buffer Kit is designed for separation of small- to medium-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MES SDS Running Buffer (20X, 500 mL, Cat. No. NP0002)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

Novex™ IEF Cathode Buffer pH 3-10 (10X) (Invitrogen™)

Novex® IEF Cathode Buffer pH 3-10 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

Pierce™ Lane Marker Reducing Sample Buffer (Thermo Scientific™)

Thermo Scientific Pierce Lane Marker Reducing Sample Buffer is a ready-to-use 5X SDS-PAGE sample loading buffer in a reducing formulation, with a pink dye buffer-front marker. This Sample Buffer contains DTT as the reducing agent, eliminating the strong odor associated with mercaptoethanol-containing buffers.

Features of Lane Marker Sample Buffer:

Easy to see—bright pink dye marks the buffer front, clearly indicating the progress of the electrophoresis run
Concentrated—5X stocks enable a greater range of protein sample volumes to be used for electrophoresis
Transferable marker dye—pink dye transfers with protein to nitrocellulose membrane, enabling verification of transfer direction and orientation of the blot

Lane Marker Reducing Sample Buffer is convenient and ready to use for SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blotting. Formulated as a 5X stock rather than the traditional 2X stock, it enables a larger volume of protein solution to be included in the sample that is loaded in each well. Particularly unique in this buffer is the use of a bright pink tracking dye instead of the traditional bromophenol blue dye. This pink dye marks not only the dye front in the gel but also transfers and permanently binds to nitrocellulose membranes. Consequently, the dye can be used to assess transfer efficiency and align cut membranes.

Lane Marker Sample Buffer is easy to use. Simply mix one part Sample Buffer with four parts protein sample, heat denature, and then load the gel sample wells. Visualize progress of the electrophoresis run by monitoring the location of the pink dye front. Lane Marker Sample Buffer may be used in denaturing gels of all kinds and is compatible with coomassie dye and silver staining techniques, as well as Western blotting procedures.

Note: This product is not compatible with fluorescent detection systems. (The pink tracking dye fluoresces strongly.)

Related Products
Pierce™ Lane Marker Non-Reducing Sample Buffer

Novex™ Tris-Glycine SDS Running Buffer (10X) (Invitrogen™)

Novex Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Separate native or denatured proteins
Novex Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex Tris-Glycine gels, use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. To separate native proteins use Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

See all available buffers and reagents available for SDS-PAGE

20X Bolt™ MOPS SDS Running Buffer (Invitrogen™)

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

Pierce™ 10X Tris-Glycine Buffer (Thermo Scientific™)

Thermo Scientific Pierce 10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for Western blotting.

Features of Thermo Scientific Pierce 10X Tris-Glycine Buffer:

Tris-glycine buffer—diluted 10-fold in water or 20% methanol, the solution yields 0.025M Tris, 0.192M glycine, pH 8.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Required:
• Methanol or ethanol as required by specific protocols.

Applications:
• Transfer buffer for Tris-glycine gel electrophoresis

Related Products
BupH™ Tris-Glycine Buffer Packs

Cathode Buffer Container (CBC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Cathode Buffer Container (CBC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The container has two separate compartments: the left provides the cathode buffer for electrophoresis and the right provides for a capillary wash and spent polymer waste ejection functionality between injections. The CBC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the CBC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Novex™ Tricine SDS Sample Buffer (2X) (Invitrogen™)

Novex Tricine SDS Sample Buffer (2X) is used to prepare protein samples for denaturing gel electrophoresis using tricine gels. Novex Tricine SDS Sample Buffer is specifically formulated for optimal electrophoresis of small proteins and peptides. The sample buffer is formulated with Coomassie Blue G and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie Blue G gives a sharp dye front with Tricine SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 85°C for 2 minutes for optimal results.

Recommended buffers: Use tricine running buffers with tricine sample buffers to obtain the benefits of this gel system.

See all buffers and reagents for SDS-PAGE ›

NuPAGE™ MOPS SDS Running Buffer (20X) (Invitrogen™)

NuPAGE MOPS SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ Transfer Buffer (20X) (Invitrogen™)

NuPAGE Transfer Buffer (20X) is used to transfer proteins from NuPAGE Bis-Tris and NuPAGE Tris-Acetate gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

NuPAGE Antioxidant may be used with NuPAGE Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

NativePAGE™ Running Buffer (20X) (Invitrogen™)

NativePAGE™ Running Buffer (20X) is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.

NuPAGE™ MES SDS Running Buffer (20X) (Invitrogen™)

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

NuPAGE™ Tris-Acetate SDS Buffer Kit (for Tris-Acetate Gels), Contains 1 ea. LA0041, NP0004, NP0005, NP0007 (Invitrogen™)

NuPAGE Tris-Acetate SDS Buffer Kit is designed for separation of medium to large size proteins on Tris-Acetate gels. This kit includes the following buffers:
• NuPAGE Tris-Acetate SDS Running Buffer (20X, Cat. No. LA0041)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

See all available buffers and reagents available for SDS-PAGE

4X Bolt™ LDS Sample Buffer (Invitrogen™)

Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

Bolt LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: Bolt LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. Bolt LDS Sample Buffer also contains a higher concentration glycerol.

Pierce™ 10X Western Blot Transfer Buffer, Methanol-free (Thermo Scientific™)

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

Features of 10X Western Blot Transfer Buffer, Methanol-free:

Transfer Buffer—diluted 10-fold in water, the solution is ready to use for electrophoresis (i.e., wet tank transfer from mini gels)
Easy to use—no packets to open, no powder to dissolve, and no methanol required
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Western Blot Transfer Buffer is ready to use upon dilution to 1X with water; it does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.

Applications:
• Wet (tank) western blot transfer of proteins to a membrane

NuPAGE™ LDS Sample Buffer (4X) (Invitrogen™)

NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol.

E-PAGE™ Loading Buffer 1 (Invitrogen™)

The E-PAGE™ High-Throughput (HTP) Protein Electrophoresis System is the only system available for fast, bufferless, high-throughput protein analysis. It consists of E-PAGE™ 96 gels, E-Base™ integrated devices, the E-Holder™ platform, and the free E-Editor™ 2.0 software.

E-PAGE™ 96 Gels
E-PAGE™ 96 gels (Table 1) are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each E-PAGE™ 96 gel contains 96 sample lanes and 8 marker lanes in a patented staggered well format with a 1.6-cm run length (Figure 1). The well openings of the E-PAGE™ 96 cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic devices (Figure 2). E-PAGE™ 96 cassettes are conveniently opened with a butterfly opener to remove the gel for staining or blotting (Figures 3 and 4). In addition, each E-PAGE™ 96 cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

E-Base™ Integrated Devices
E-PAGE™ 96 gels run in the specially designed E-Base™ electrophoresis bases, which combine the base and the power source in one device. Two types of bases are available from Invitrogen:
1. The mother E-Base™ device contains an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-PAGE™ 96 gel
2. The daughter E-Base™ device connects to the mother E-Base™ (Figure 5) and to other daughter E-Base™ devices for the simultaneous electrophoresis of two or more E-PAGE™ 96 gels. The daughter base does not contain an electrical plug and cannot be used without a mother base

Each base has a power/program button and a timer button on the lower right side of the base for controlling your electrophoresis run. The lower left side of each base contains a lighted LED and a digital timer display (00-99) that indicates where your gel is in the running process. Two programs are pre-set in the E-Base™ units: a 14-minute protein run program for E-PAGE™ 96 gels and a 12-minute DNA program for running E-Gel® 96 Gels. For E-Gel® 48 Gels, use the DNA program extended to 20 minutes due to the longer running distance.

Note: The E-Base™ can run either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 systems. The E-PAGE™ 96 gel is not compatible with current models of the E-Gel® 96 bases (mother and daughter bases).

E-Holder™ platform
The E-Holder™ platform is compatible in size with standard automated systems for convenient loading of samples in a hands-free robot environment when the use of the E-Base™ devices is not practical or while in queue for its availability.

E-Editor™ 2.0 Software
Analysis of E-PAGE™ 96 gel results is fast and convenient, for both stained gels and blots, using the Window®-based E-Editor™ 2.0 software. All 96 lanes (and the additional 8 marker lanes) are grouped and aligned into one image, for easy interpretation and comparison. Moreover, when 384-well assays are performed, the E-Editor™ program allows selection of multiple automated loading patterns and grouping of four different E-PAGE™ 96 gel results into one integrated image. This enables an immediate reference back from the gel or blot lane into the coordinates of the originating plate well to provide quick identification of important results.

NativePAGE™ Sample Buffer (4X) (Invitrogen™)

NativePAGE™ Sample Buffer (4X) is a solution used to solubilize native protein samples prior to use with the NativePAGE™ Gel System. The buffer is used along with 10% DDM or 5% Digitonin detergents to solubilize hydrophobic or membrane proteins. This buffer is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

Novex™ IEF Sample Buffer pH 3-10 (2X) (Invitrogen™)

Novex® IEF Sample Buffer pH 3-10 (2X) is optimized pre-mixed IEF sample buffer for using with Novex® pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Bolt™ Transfer Buffer (20X) (Invitrogen™)

Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

Bolt Antioxidant may be used with Bolt Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE