Shop All Protein Gel Buffers

Novex™ pH 3-7 IEF Buffer Kit, Includes LC5300, LC5370, LC5371 (Invitrogen™)

Novex® pH 3-7 IEF Buffer Kit is optimized for using with Novex® pH 3-7 IEF gels. Each kit includes LC5300, LC5370, LC5371

NativePAGE™ Running Buffer Kit (Invitrogen™)

The NativePAGE™ Running Buffer Kit contains the NativePAGE™ Running Buffer (20X), which is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels. The kit also contains the NativePAGE™ Cathode Buffer Additive (20X) that is used with the NativePAGE™ Running Buffer to make the Cathode Running Buffer for the system.

20X Bolt™ MOPS SDS Running Buffer (Invitrogen™)

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

Fluorescent Compatible Sample Buffer (4X, non-reducing) (Invitrogen™)

Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is optimized for use in fluorescent western blotting as it does not interfere in fluorescent channels, unlike other standard sample buffers. Fluorescent Compatible Sample Buffer is non-reducing but may also be used under denaturing conditions.

Non-reducing—ready-to-use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
Lane marker dyes—visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
Concentrated—4X formulation provides more versatility than traditional 2X buffers
Ideal for fluorescent detection—formulated to prevent auto fluorescence during fluorescent detection

Novex™ Tris-Glycine SDS Buffer Kit (Invitrogen™)

Pre-mixed buffers are a convenient way to ensure high-quality, consistent electrophoresis results. All buffers are made with high-purity reagents and are strictly quality controlled. The buffers are provided as a concentrated solution requiring a simple dilution with deionized water before use.

Bolt™ Transfer Buffer (20X) (Invitrogen™)

Bolt Transfer Buffer (20X) is used to transfer proteins from Bolt Bis-Tris Plus gels to membranes for western blotting. It maintains the neutral pH environment established during electrophoresis. The neutral pH protects against modification of amino acid side chains and is compatible with N-terminal protein sequencing using Edman degradation.

Bolt Antioxidant may be used with Bolt Transfer Buffer to enhance transfer of reduced proteins to membranes.

See all available buffers and reagents available for SDS-PAGE

Novex™ IEF Cathode Buffer pH 3-10 (10X) (Invitrogen™)

Novex® IEF Cathode Buffer pH 3-10 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-10 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

NuPAGE™ MES SDS Running Buffer (20X) (Invitrogen™)

NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. It is recommended for separating small- to medium-sized proteins.

Use the right buffer to optimize protein separations
NuPAGE MES SDS Running Buffer and NuPAGE MOPS SDS Running Buffer can both be used with NuPAGE Bis-Tris gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

NativePAGE™ Running Buffer (20X) (Invitrogen™)

NativePAGE™ Running Buffer (20X) is used to make the NativePAGE™ Cathode and Anode Running Buffers for use with an XCell SureLock® Mini Cell when running NativePAGE™ Gels.

Novex™ pH 3-10 IEF Buffer Kit,Includes LC5300, LC5310, LC5311 (Invitrogen™)

Novex® pH 3-10 IEF Buffer Kit is optimized for using with Novex® pH 3-10 IEF gels. Each kit includes LC5300, LC5310, LC5311.

Pierce™ 1-Step Transfer Buffer (Thermo Scientific™)

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Features of 1-Step Transfer Buffer:

Fast—high ionic strength formulation provides 5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems
Ready to use—supplied as a 1X solution that is stable and ready to use at room temperature
Optimized—designed for seamless and reliable performance with the Pierce G2 Fast Blotter
Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217)
Economical—excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices

When used with the Pierce G2 Fast Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217), when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Requires:
Pierce G2 Fast Blotter (Part No. 62288) or other semi-dry transfer device that is equipped or paired with a capable high-current power supply.

Fast blotting methods require a high-current power supply, such as the Pierce G2 Fast Blotter, and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (~22-23mA/cm2) and voltage is limited to 25V.

NuPAGE™ LDS Sample Buffer (4X) (Invitrogen™)

NuPAGE LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris or Tris-Acetate gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.

See all available buffers and reagents available for SDS-PAGE

NuPAGE LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.

To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.

Note: NuPAGE LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. NuPAGE LDS Sample Buffer also contains a higher concentration glycerol.

Novex™ Tris-Glycine Native Sample Buffer (2X) (Invitrogen™)

Novex Tris-Glycine Native Sample Buffer (2X) is designed for protein gel electrophoresis under native running conditions with Tris-Glycine gels or Tris-Acetate gels. It has a pH of 8.6 and contains bromophenol blue as a tracking dye.

See all available buffers and reagents available for Native-PAGE

Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 (Invitrogen™)

The Novex Tricine SDS Buffer Kit is designed for separation of small- to medium-size proteins on tricine gels. This kit includes the following buffers:
• Tricine SDS Sample Buffer (Cat. No. LC1676)
• Novex Tricine SDS Running Buffer (Cat. No. LC1675)

See all buffers and reagents for SDS-PAGE ›

Pierce™ 10X Western Blot Transfer Buffer, Methanol-free (Thermo Scientific™)

Thermo Scientific Pierce 10X Western Blot Transfer Buffer, Methanol-free is a space-saving stock solution for preparing the methanol-free transfer buffer used with cooled, tank (wet) western blotting transfer apparatus.

Features of 10X Western Blot Transfer Buffer, Methanol-free:

Transfer Buffer—diluted 10-fold in water, the solution is ready to use for electrophoresis (i.e., wet tank transfer from mini gels)
Easy to use—no packets to open, no powder to dissolve, and no methanol required
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—10X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 10X Western Blot Transfer Buffer is ready to use upon dilution to 1X with water; it does not contain methanol and the addition of methanol is not necessary for use in electrophoresis.

Applications:
• Wet (tank) western blot transfer of proteins to a membrane