Shop All Protein Gel Buffers

Novex™ Zymogram Developing Buffer (10X) (Invitrogen™)

Novex® Zymogram Gels are excellent tools for detecting and characterizing proteases that utilize casein or gelatin as a substrate. Proteases are run on a gel containing one of these substrates under denaturing conditions and visualized as clear bands against a dark background following a simple renaturing, developing, and staining protocol. Zymogram gels are commonly used to detect matrix metalloproteinases.

Formulation: All Novex® Zymogram Gels are Tris-Glycine gels with a substrate (gelatin or casein) incorporated into the gel. They are made with high-purity, strictly quality-controlled reagents: Tris base, HCl, acrylamide, bisacrylamide, TEMED, APS, highly purified water and casein or gelatin as the substrate. All have a 4% stacking gel.

Zymogram Gel Types: The 10% Zymogram (Gelatin) Gel is a 10% Tris-Glycine gel with 0.1% gelatin as the substrate. The 12% Novex® Zymogram (Casein) Gel is a 12% Tris-Glycine gel with 0.05% casein as the substrate. The 4-16% Novex® Zymogram (Blue Casein) Gel is a 4-16% Novex® Tris-Glycine gel with 0.1% casein as the substrate and a proprietary dye incorporated throughout the gel.

Sensitivity Level:
10% Zymogram Gelatin Gel: 5 x 10-6 units of collagenase
12% Zymogram Casein Gel: 7 x 10-4 units of trypsin
4-16% Zymogram Casein Gel: 1.5 x 10-3 units of trypsin

Recommended Buffers: Use Novex® Tris-Glycine SDS Sample Buffer and Novex® Tris-Glycine SDS Running Buffer with these gels. Novex® Zymogram Renaturing and Developing Buffers are also recommended.

Novex™ Tris-Glycine SDS Running Buffer (10X) (Invitrogen™)

Novex Tris-Glycine SDS Running Buffer (10X) is formulated for separation of proteins in their denatured state on Tris-Glycine gels. Tris-Glycine gels provide reproducible separation of a wide range of proteins into well-resolved bands.

Separate native or denatured proteins
Novex Tris-Glycine gels do not contain SDS and can be used to accurately separate both native and denatured proteins, depending upon the sample and running buffers used. To separate denatured proteins on Novex Tris-Glycine gels, use Novex Tris-Glycine SDS Sample Buffer and Novex Tris-Glycine SDS Running Buffer. To separate native proteins use Novex Tris-Glycine Native Sample Buffer and Novex Tris-Glycine Native Running Buffer.

See all available buffers and reagents available for SDS-PAGE

Novex™ IEF Anode Buffer (50X) (Invitrogen™)

Novex® IEF Anode Buffer (50X) is optimized pre-mixed IEF anode buffer for using with Novex® IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Anode Buffer Container (ABC) for 3500 Series Systems for Protein Quality Analysis (Applied Biosystems™)

The Anode Buffer Container (ABC) contains 1X running buffer to support protein quality analysis applications on the Applied Biosystems™ 3500 Series genetic analyzers for protein quality analysis. The ABC is ready-to-use and disposable with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat-sealed with a plastic film, which is removed prior to installation on the instrument. Each package includes four containers.

Novex™ IEF Cathode Buffer pH 3-7 (10X) (Invitrogen™)

Novex® IEF Cathode Buffer pH 3-7 (10X) is optimized pre-mixed IEF cathod buffer for using with Novex® pH 3-7 IEF gels. IEF is a sensitive technique which is affected by many factors. The optimized pre-mixed IEF buffers reduce variability and ensure consistent results.

Usage note: The IEF cathode buffer solutions contain a reagent that varies from white to light yellow in color. This color variation may produce a similar color variation in the final solution, from colorless to yellowish in tone. This color will not affect product performance.

NativePAGE™ Sample Buffer (4X) (Invitrogen™)

NativePAGE™ Sample Buffer (4X) is a solution used to solubilize native protein samples prior to use with the NativePAGE™ Gel System. The buffer is used along with 10% DDM or 5% Digitonin detergents to solubilize hydrophobic or membrane proteins. This buffer is also included in the NativePAGE™ Sample Prep Kit. The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis showing increased resolution and reduced streaking.

Fluorescent Compatible Sample Buffer (4X, non-reducing) (Invitrogen™)

Invitrogen Fluorescent Compatible Sample Buffer is for use in SDS-polyacrylamide gel electrophoresis (SDS-PAGE). It is optimized for use in fluorescent western blotting as it does not interfere in fluorescent channels, unlike other standard sample buffers. Fluorescent Compatible Sample Buffer is non-reducing but may also be used under denaturing conditions.

Non-reducing—ready-to-use for non-reducing SDS-PAGE, or the preferred type and amount of reducing agent (e.g., DTT) can be added to produce reducing conditions
Lane marker dyes—visible markers of the dye front
Convenient—stable at room temperature, enabling storage at the bench where electrophoresis is performed
Concentrated—4X formulation provides more versatility than traditional 2X buffers
Ideal for fluorescent detection—formulated to prevent auto fluorescence during fluorescent detection

NuPAGE™ MOPS SDS Buffer Kit (for Bis-Tris Gels) (Invitrogen™)

The NuPAGE MOPS SDS Buffer Kit is designed for separation of medium- to large-size proteins on NuPAGE Bis-Tris gels and includes the following buffers:
• NuPAGE MOPS SDS Running Buffer (20X, 500 mL, Cat. No. NP0001)
• NuPAGE Sample Reducing Agent (10X, 250 µL, Cat. No. NP0004)
• NuPAGE Antioxidant (Cat. No. NP0005)
• NuPAGE LDS Sample Buffer (4X, 10 mL, Cat. No. NP0007)

Compare protein migration patterns using MES and MOPs on NuPAGE Bis-Tris gels

See all available buffers and reagents available for SDS-PAGE

Pierce™ 1-Step Transfer Buffer (Thermo Scientific™)

Thermo Scientific Pierce 1-Step Transfer Buffer is designed for rapid semi-dry transfer of 10-300kDa proteins from polyacrylamide gels (SDS-PAGE) to nitrocellulose or PVDF membranes using the Pierce G2 Fast Blotter.

Features of 1-Step Transfer Buffer:

Fast—high ionic strength formulation provides 5- to 10-minute protein transfer when used with compatible fast semi-dry blotting systems
Ready to use—supplied as a 1X solution that is stable and ready to use at room temperature
Optimized—designed for seamless and reliable performance with the Pierce G2 Fast Blotter
Compatible—effective with other semi-dry transfer devices equipped with a suitable high-current power supply, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217)
Economical—excellent performance without the special or costly consumables that are required by other fast semi-dry transfer devices

When used with the Pierce G2 Fast Blotter, this buffer provides effective gel-to-membrane transfer of proteins in 5 to 10 minutes with efficiency that is equal to, or better than, conventional Western blotting techniques. Pierce 1-Step Transfer Buffer is also compatible with other protein semi-dry transfer devices, including the original Pierce Fast Semi-Dry Blotter (Part No. 88217), when they are paired with a suitable high-current power supply. Such devices provide constant high current (1.3 to 5.0 amps) to rapidly transfer proteins via the high ionic strength conditions supplied by the transfer buffer.

Pierce G2 Fast Blotter (Part No. 62288) or other semi-dry transfer device that is equipped or paired with a capable high-current power supply.

Fast blotting methods require a high-current power supply, such as the Pierce G2 Fast Blotter, and an optimized high ionic strength transfer buffer, such as Pierce 1-Step Transfer Buffer. By increasing the current, excellent transfer efficiency can be achieved in much shorter time compared to conventional methods. Amperage is held at a constant rate based on the surface area of the transfer stack(s) (~22-23mA/cm2) and voltage is limited to 25V.

20X Bolt™ MOPS SDS Running Buffer (Invitrogen™)

Bolt MOPS SDS Running Buffer (20X) is formulated for running proteins on Bolt Bis-Tris Plus gels. It is recommended for separating medium- to large-sized proteins.

Use the right buffer to optimize protein separations
Bolt MES SDS Running Buffer and Bolt MOPS SDS Running Buffer can both be used with Bolt Bis-Tris Plus gels. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. The difference in ion migration affects stacking and results in a difference in protein separation range between these buffers. Use of MOPS buffer allows proteins to run slower than when using MES buffer.

Compare protein migration patterns using MES and MOPs on Bolt Bis-Tris Plus gels

See all available buffers and reagents available for SDS-PAGE

E-PAGE™ Loading Buffer 1 (Invitrogen™)

The E-PAGE™ High-Throughput (HTP) Protein Electrophoresis System is the only system available for fast, bufferless, high-throughput protein analysis. It consists of E-PAGE™ 96 gels, E-Base™ integrated devices, the E-Holder™ platform, and the free E-Editor™ 2.0 software.

E-PAGE™ 96 Gels
E-PAGE™ 96 gels (Table 1) are self-contained, pre-cast gels that include a gel matrix and electrodes packaged inside a disposable, UV-transparent cassette. Each E-PAGE™ 96 gel contains 96 sample lanes and 8 marker lanes in a patented staggered well format with a 1.6-cm run length (Figure 1). The well openings of the E-PAGE™ 96 cassette are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid-handling robotic devices (Figure 2). E-PAGE™ 96 cassettes are conveniently opened with a butterfly opener to remove the gel for staining or blotting (Figures 3 and 4). In addition, each E-PAGE™ 96 cassette is labeled with an individual barcode to facilitate identification of the gel using commercial barcode readers.

E-Base™ Integrated Devices
E-PAGE™ 96 gels run in the specially designed E-Base™ electrophoresis bases, which combine the base and the power source in one device. Two types of bases are available from Invitrogen:
1. The mother E-Base™ device contains an electrical plug that can be connected directly to an electrical outlet and is used for electrophoresis of one E-PAGE™ 96 gel
2. The daughter E-Base™ device connects to the mother E-Base™ (Figure 5) and to other daughter E-Base™ devices for the simultaneous electrophoresis of two or more E-PAGE™ 96 gels. The daughter base does not contain an electrical plug and cannot be used without a mother base

Each base has a power/program button and a timer button on the lower right side of the base for controlling your electrophoresis run. The lower left side of each base contains a lighted LED and a digital timer display (00-99) that indicates where your gel is in the running process. Two programs are pre-set in the E-Base™ units: a 14-minute protein run program for E-PAGE™ 96 gels and a 12-minute DNA program for running E-Gel® 96 Gels. For E-Gel® 48 Gels, use the DNA program extended to 20 minutes due to the longer running distance.

Note: The E-Base™ can run either E-Gel® 96, E-Gel® 48, or E-PAGE™ 96 systems. The E-PAGE™ 96 gel is not compatible with current models of the E-Gel® 96 bases (mother and daughter bases).

E-Holder™ platform
The E-Holder™ platform is compatible in size with standard automated systems for convenient loading of samples in a hands-free robot environment when the use of the E-Base™ devices is not practical or while in queue for its availability.

E-Editor™ 2.0 Software
Analysis of E-PAGE™ 96 gel results is fast and convenient, for both stained gels and blots, using the Window®-based E-Editor™ 2.0 software. All 96 lanes (and the additional 8 marker lanes) are grouped and aligned into one image, for easy interpretation and comparison. Moreover, when 384-well assays are performed, the E-Editor™ program allows selection of multiple automated loading patterns and grouping of four different E-PAGE™ 96 gel results into one integrated image. This enables an immediate reference back from the gel or blot lane into the coordinates of the originating plate well to provide quick identification of important results.

NativePAGE™ Cathode Buffer Additive (20X) (Invitrogen™)

NativePAGE™ Cathode Buffer Additive (20X) is added to the NativePAGE™ Running Buffer and the resulting buffer is used in the cathode chamber of an XCell SureLock® Mini Cell for running NativePAGE™ Gels.

NativePAGE™ Sample Prep Kit (Invitrogen™)

The NativePAGE™ Sample Prep Kit includes sample preparation reagents for native gel electrophoresis. The kit includes two ready-to-use detergent solutions, 10% DDM (n-dodecyl-β-D-maltoside) and 5% Digitonin, which improve the solubility of hydrophobic and membrane proteins during sample preparation.

The samples prepared with 10% DDM, 5% Digitonin, or the NativePAGE™ Sample Prep Kit are compatible with NativePAGE™ Novex Bis-Tris Gels for native electrophoresis, showing increased resolution and reduced streaking.

Novex™ Tris-Glycine Transfer Buffer (25X) (Invitrogen™)

Novex Tris-Glycine Transfer Buffer (25X) is optimized for western blot transfer of proteins to nitrocellulose, PVDF, or nylon membranes for subsequent analysis using Tris-Glycine gels.

To use: This buffer should be diluted to a 1X solution with a water/methanol mixture to yield a final methanol concentration of 20% for optimal results.

See all available buffers and reagents available for SDS-PAGE

Novex™ Tricine SDS Buffer Kit, includes LC1676 & LC1675 (Invitrogen™)

The Novex Tricine SDS Buffer Kit is designed for separation of small- to medium-size proteins on tricine gels. This kit includes the following buffers:
• Tricine SDS Sample Buffer (Cat. No. LC1676)
• Novex Tricine SDS Running Buffer (Cat. No. LC1675)

See all buffers and reagents for SDS-PAGE ›