Shop All Acrylamide & Polymerizing Agents

UltraPure™ TEMED (Invitrogen™)

UltraPure™ TEMED (N,N,N´,N´-tetramethylethylenediamine) is the most commonly used accelerator for the polymerization of acrylamide gels.

Rhinohide™ Polyacrylamide Gel Strengthener Concentrate (Invitrogen™)

Rhinohide polyacrylamide gel strengthener improves upon classic polyacrylamide gel technology by making gels much stronger, providing easier handling and resistance to tearing, without adverse side effects. Rhinohide polyacrylamide gel strengthener is highly recommended for low-percentage gels, large-format gels and gels subject to multiple staining and handling steps. Rhinohide polyacrylamide gel strengthener is also available in a kit (R-33410), which includes an acrylamide/bis-acrylamide mixture (37.5:1 ratio).

SureCast™ Resolving Buffer (Invitrogen™)

SureCast Resolving Buffer packs are pouches of dry blend powder each sufficient to make 500 mL of resolving gel buffer (1.5 M Tris buffer, pH 8.8) for hand-casting polyacrylamide gels. They are easy to use—simply empty the contents of one pouch into a beaker, add ultrapure water, and stir to dissolve.

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Acrylamide/Bis 19:1, 40% (w/v) solution (Invitrogen™)

Ambion® Acrylamide/Bis 19:1 is a 40% (w/v) solution of acrylamide (38%) and bis-acrylamide (2%) ideal for use in ribonuclease protection assay, sequencing gels, and sizing DNA or RNA fragments. Supplied in one bottle containing 500 mL. The solution is provided in a ready-to-use form, reducing the dust, inhalation, and contact hazard associated with weighing and preparing acrylamide and bis-acrylamide powders and solutions.

UltraPure™ N,N'-Methylenebisacrylamide (Invitrogen™)

UltraPure™ N,N´-Methylenebisacrylamide is the most frequently used cross-linking agent for polyacrylamide gels.

Performance and Quality Testing: Polymerization time is evaluated in an SDS polyacrylamide gel system.

Ammonium Persulfate (Thermo Scientific™)

Thermo Scientific Pierce Ammonium Persulfate (APS) is an oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

Ammonium persulfate (APS) is an oxidizing agent that is often used with tetramethylethylenediamine (TEMED, Part No. 17919) to catalyze the polymerization of acrylamide and bisacrylamide to prepare polyacrylamide gels for electrophoresis.

Details:

• Name: Ammonium persulfate (APS)
• Formula: (NH4)2S2O8
• CAS number: 7727-54-0
• Molecular weight: 228.2
• Purity: ≥98.0%
• Insolubles: ≤0.005%

TEMED (Thermo Scientific™)

Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) is an essential catalyst for polyacrylamide gel polymerization. TEMED is used with ammonium persulfate (APS) to catalyze acrylamide polymerization when preparing gels for electrophoresis.

Features of Thermo Scientific Pierce Tetramethylethylenediamine (TEMED) :

• Chemical name: N,N,N',N'-tetramethylethane-1,2-diamine
• Formula: C6H16N2
• CAS number: 110-18-9
• Molecular weight: 116.21
• Purity: ≥99%
• Refractive index: 1.417 to 1.419
• Boiling range: 119 to 121°C
• Clear liquid at room temperature

SureCast™ Stacking Buffer (Invitrogen™)

SureCast Stacking Buffer packs are pouches of dry blend powder, each sufficient to make 500 mL of stacking gel buffer (0.5 M Tris-HCl buffer, pH 6.8) for hand-casting polyacrylamide gels. They are easy to use—simply empty the contents of one pouch into a beaker, add ultrapure water, and stir to dissolve.

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SureCast™ Acrylamide Solution (40%) (Invitrogen™)

SureCast Acrylamide Solution (40%) can be used to prepare single-percentage and gradient gels using the SureCast Handcast System or in other hand-casting systems. Acrylamide is a major ingredient in polyacrylamide gels used for separating proteins during polyacrylamide gel electrophoresis (PAGE).

Features of the SureCast Acrylamide Solution include:
• Bis-acrylamide ratio of 29:1
• Room temperature storage
• Long shelf life
• High purity
• Safer alternative to powdered acrylamide
• Concentrated to enable the hand-casting of a broad range of gel percentages

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UltraPure™ Acrylamide (Invitrogen™)

UltraPure™ Acrylamide is a major ingredient in polyacrylamide gels, which are used for electrophoretic separation of both nucleic acid fragments and proteins. DNA fragments generated by restriction endonuclease digestion, dideoxy sequencing, or the Maxam and Gilbert sequencing methods can be separated by polyacrylamide gel electrophoresis (PAGE). Proteins can be separated by a variety of techniques with polyacrylamide gels, including denaturing gels using SDS or urea, isoelectric focusing gels, and native gels in a wide variety of buffers. UltraPure™ Acrylamide is recrystallized three times to ensure a highly purified product.

Performance and Quality Testing: Polymerization time is evaluated in an SDS polyacrylamide gel system.

SureCast™ TEMED (Invitrogen™)

SureCast TEMED (N,N,N',N'-tetramethylethylenediamine) is an accelerator for the polymerization of acrylamide and bis-acrylamide in the preparation of polyacrylamide gels for electrophoresis.

See all gel casting reagents ›
Learn more about the SureCast Handcast system ›

SureCast™ Ammonium Persulfate (APS) (Invitrogen™)

SureCast Ammonium Persulfate (APS) is an oxidizing agent that is used with tetramethylethylenediamine (TEMED) to catalyze the polymerization of acrylamide and bis-acrylamide in the preparation of polyacrylamide gels for electrophoresis.

See all gel casting reagents ›
Learn more about the SureCast Handcast system ›