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Water For Injection (WFI) for Cell Culture (Gibco™)

Gibco™ Water For Injection (WFI) for Cell Culture is high-quality water for use as a solvent in the preparation of cell culture media, laboratory reagents, buffers, and bioprocess liquids.

Some features of WFI for Cell Culture include:
• Water For Injection and USP/EP quality
• Gibco quality and performance
• Can be used in cell culture, molecular biology, and upstream and downstream bioprocessing applications

Water For Injection quality
WFI for Cell Culture meets USP/EP monograph for Water For Injection at point of fill. The water is prepared by distillation and filtration (0.1 µm), with no added substances. Water quality is monitored on a routine basis. Conductivity, total organic carbon, endotoxin, and bioburden are tested daily; metals are tested monthly.

Gibco quality and performance
The performance and consistency expected from Gibco liquid media products depends on the high-quality water used in manufacturing. This water is packaged in bottles and CX5-14 bioprocess containers as Gibco Water For Injection (WFI) for Cell Culture. In addition to meeting USP/EP standards, each lot is quality tested for sterility, endotoxin, conductivity, pH, osmolality, nitrates, and total organic carbon.

Use in cell culture and molecular biology applications
WFI for Cell Culture can be used for a wide variety of applications, including preparing powder or Advanced Granulation Technology™ (AGT™) media, making solutions, diluting buffers, and cleaning and rinsing agents.

Product use
For use as a raw material component in further manufacturing applications.

cGMP manufacturing and quality system
WFI for Cell Culture is manufactured at cGMP-compliant facilities located in Grand Island, New York and Inchinnan, Scotland. Both facilities are certified to ISO 13485 cGMP manufacturing standards.

UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen™)

UltraPure™ DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-µm membrane-filtered and tested for DNase and RNase activity.

Performance and quality testing
No DNase, RNase, or protease activity detected. Our distilled water system is routinely monitored for compliance with current USP monograph test requirements for Water for Injection (WFI).

Specialty Waters (Thermo Scientific™)

Manufactured, packaged and tested to exacting standards, these specialty waters are highly purified low TOC water in 1L bottles or 4L glass jugs.

Nuclease-Free Water (not DEPC-Treated) (Invitrogen™)

Nuclease-free Water (not DEPC-treated) has been deionized, filtered into the final bottle, and autoclaved. It is ready to use and requires no preparation, mixing, or autoclaving. This nuclease-free water is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Using Nuclease-free Water
Because this water is not DEPC-treated, it is recommended for applications for which residual DEPC might prove detrimental (e.g., oocyte microinjection).

Distilled Water (Gibco™)

This distilled water is high-quality water for use as a solvent in the preparation of cell culture media and laboratory reagents. Available in many sizes to meet your needs, this distilled water features:

• Cell culture quality
• Proven performance
• Suitable for use in cell culture and molecular biology applications

Cell culture quality
This water is prepared by distillation and filtration (0.1 µm), with no added substances. We monitor the water quality on a routine basis. Conductivity, total organic carbon, endotoxin, and bioburden are tested daily; metals are tested monthly.

Proven performance
The performance and consistency expected from Gibco® liquid media products depends on the high-quality water we use in manufacturing. This water is also bottled as Gibco® Distilled Water.

Use in cell culture and molecular biology applications
This distilled water can be used for a wide variety of applications, including preparing powder or Advanced Granulation Technology™ (AGT™) media, making solutions, and diluting buffers.

Dual-site cGMP manufacturing and quality system
This water is produced at a cGMP-compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical product made in our Grand Island facility (15230-162). This facility is also registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

UltraPure™ DEPC-Treated Water (Invitrogen™)

UltraPure™ DEPC-treated Water is suitable for use with RNA. It is prepared by incubating with 0.1% diethylpyrocarbonate (DEPC), and is then autoclaved to remove the DEPC. Sterile filtered.

Performance and Quality Testing: No DNase or RNase activity detected.

DEPC-Treated Water (Invitrogen™)

Ambion® DEPC-treated water is certified nuclease-free and supplied in one bottle containing 1000 mL. DEPC-treated water is autoclaved pre- and post-packaging to ensure sterility and inactivation of DEPC. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

RT-PCR Grade Water (Invitrogen™)

RT-PCR Grade Water is certified free of nucleases, and free of nucleic acid contamination that may cause false-positive signals in RT-PCR. The RT-PCR Grade Water is ready to use and requires no preparation, mixing, or autoclaving.

Quality control
RT-PCR Grade Water is tested for prokaryotic as well as eukaryotic genomic DNA contamination using an ultrasensitive PCR assay. It is provided in 10 x 1.5 mL tubes. Prokaryotic DNA contamination is assessed by performing PCR with primers to 16S rRNA. Eukaryotic DNA contamination is assessed by performing PCR with primers to 18S rRNA. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity and is suitable for any PCR application.

Water, nuclease-free (Thermo Scientific™)

Thermo Scientific nuclease-free water is deionized and 0.22 µm membrane-filtered nuclease-free water. It is ideal for all molecular biology applications.

Features
• Free of endo-, exodeoxyribonucleases, ribonucleases, and phosphatases

Covaris™ M220 AFA-grade Water (Ion Torrent™)

Highly purified water (at least ASTM Type III or ISO Grade 3) is required when using the M220 Focused-ultrasonicator™. One AFA-grade water bottle (800 ml) is sufficient for more than 50 uses.

Nuclease-free Water (1.75 ml/tube)

Nuclease-free water for use in RNA applications.

For Research Use Only. Not for use in diagnostics procedures.