Shop All DNA⁄RNA Reagents

QuantiGene™ Lysis Mixture (Invitrogen™)

QuantiGene Lysis Mixture is used to lyse cells from cell culture or whole blood, in order to release target RNA to be quantified with QuantiGene assays. QuantiGene Lysis Mixture is one of two components in the QuantiGene Sample Processing kits for cells and whole blood, and is intended to supplement these kits when additional volume is required.

Human Brain Reference RNA (Invitrogen™)

Human Brain Total RNA is a total RNA sample extracted from neural tissue for use as a positive control in QuantiGene assays.

Yeast tRNA (Invitrogen™)

Yeast tRNA is suitable for use as a carrier in nucleic acid purification and precipitation procedures. It is supplied in lyophilized form.

Breast Adenocaricinoma (MCF-7) Total RNA (Invitrogen™)

Breast Adenocarcinoma (MCF-7) Total RNA is a total RNA sample extracted from MCF-7 cells for use as a positive control in QuantiGene assays.

Yeast RNA (10 mg/mL) (Invitrogen™)

Purified Torulla Ambion® yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant. This total RNA preparation is supplied in ten 1 mL tubes at a concentration of 10 mg/mL in diethyl pyrocarbonate-treated distilled water. It is typically used at a working concentration of 10–20 µg/mL.

What is a Coprecipitant?
Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations. While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation.

Human Colon Total RNA (Invitrogen™)

Human Colon Total RNA is a total RNA sample extracted from large intestine for use as a positive control in QuantiGene assays.

Human Kidney Total RNA (Invitrogen™)

Human Kidney Total RNA is a total RNA sample extracted from human renal tissue for use as a positive control in QuantiGene assays.

Human Pancreas Total RNA (Invitrogen™)

Human Pancreatic Total RNA is a total RNA sample extracted from human pancreatic tissue for use as a positive control in tQuantiGene assays.

Human Cot-1 DNA™ (Invitrogen™)

Human Cot-1 DNA® is commonly used to block nonspecific hybridization in microarray screening. It can also be used to suppress repetitive DNA sequences for the direct mapping of human DNA or mapping genomic clones to panels of somatic-cell hybrids for chromosome localization by Southern blotting. Human Cot-1 DNA® is effective as a library-screening probe for somatic-cell hybrid libraries and flow-sorted chromosome libraries made from somatic-cell hybrids.

About Cot-1 DNA®
Human Cot-1 DNA® is placental DNA that is predominantly 50 to 300 bp in size and enriched for repetitive DNA sequences such as the Alu and Kpn family members. The amount supplied per package is sufficient for 5–10 Southern or 500 in situ hybridizations.

Performance and quality testing
Purity and DNA size are verified by agarose gel electrophoresis. Concentration is verified spectrophotometrically by diluting Human Cot-1 DNA® 1:100 in 50 mM NaOH and using the conversion factor 0.033 µg/µL/A260. Other methods used to determine concentration may yield varying results.

Yeast tRNA (10 mg/mL) (Invitrogen™)

Ambion® Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10–20 µg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids. It is supplied in one tube containing 0.5 mL at a concentration of 10 mg/mL.

What is a Coprecipitant?
Coprecipitants are inert substances used to aid recovery of nucleic acids during alcohol precipitations. While they can be used for precipitating large amounts of nucleic acids, they are essential for quantitative recovery of small amounts of nucleic acids in dilute solutions. Often, the use of such molecules is desirable for no other reason but visualization of the pelleted precipitate after centrifugation.