Shop All Immunoassay Buffers & Reagents

ELISA/ELISPOT Coating Buffer Powder Invitrogen™

The ELISA/ELISPOT Coating Buffer Powder is designed and tested for use as the working solution for coating the ELISA or ELISPOT capture antibody to the ELISA or ELISPOT plate microwells. This has been tested with all cytokine ELISA and ELISPOT capture antibodies, according to the procedures described in the corresponding protocols.

The eBioscience ELISA/ELISPOT Coating Buffer Powder is a dried powder formulation of phosphate buffered saline. Each packet is reconstituted in 1 Liter of distilled, deionized water, then filtered using a 0.22 um filter. The resulting 1x PBS (pH 7.4) is suitable for use as the ELISA/ELISPOT coating buffer.

Reported Application
ELISA, ELISPOT

ProcartaPlex™ Cell Lysis Buffer Invitrogen™

Refer to the TDS in the Documents Section

Reported Application
Multiplex Immunoassay

SuperBlock™ (TBS) Blocking Buffer Dry Blend Thermo Scientific™

SuperBlock Blocking Buffer Dry Blend contains a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.

Compare and view all available blocking buffers

Features of SuperBlock Blocking Buffer:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer in solution at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related products
SuperBlock (PBS) Blocking Buffer
SuperBlock T20 (PBS) Blocking Buffer
SuperBlock (TBS) Blocking Buffer
SuperBlock T20 (TBS) Blocking Buffer

Human/Monkey Extracellular Protein Buffer Magnetic Reagent Kit Invitrogen™

This Human/Monkey Extracellular Protein Buffer Reagent Kit is comprised of the reagents that are intended for use with Human and Monkey extracellular magnetic singleplex kits for in vitro quantitative determination of analytes in serum, plasma, and tissue culture supernatant. Singleplex magnetic bead kits maximize flexibility in experimental design, permitting the measurement of one or multiple analytes in panels designed by the researcher. These reagents are suitable for use with the Luminex® 200™, FLEXMAP 3D®, and MAGPIX® systems.

Luminex® xMAP® Technology—Effective and Proven Analytical Tool
Human and Monkey extracellular magnetic singleplex kits are based on xMAP® technology. The use of a suspension bead-based technology enables the multiplexing capabilities of the Luminex® assays. Magnetic microspheres are internally dyed with red and infrared fluorophores of differing intensities. Each bead is given a unique number, or bead region, allowing differentiation of one bead from another. Beads covalently bound to different antibodies can be mixed in the same assay, utilizing a 96-well microplate format. At the completion of the sandwich immunoassay, beads can be read using the Luminex® 200™ or FLEXMAP 3D® detection systems, instruments based on flow cytometry, two lasers, real-time digital signal processing, and xPONENT® software to distinguish bead color (analyte) and assay signal strength (PE) fluorescence intensity.

MAGPIX® System—Brings the Power of Multiplex to Every Lab
The MAGPIX® system may also be used for sample analysis. This instrument utilizes xMAP® technology and is built on light-emitting diodes (LEDs) for excitation, a CCD camera for bead and analyte detection and high-speed digital signal processors. The MAGPIX® system is more compact and robust, providing a very cost effective magnetic bead-based multiplexing tool. Simple, out of the box set-up allows researchers to rapidly perform singleplex or multiplex assays of up to 50 targets in single biological samples. The MAGPIX® system is easy to use—ideal for both new and experienced users alike.

Magnetic Beads Let You Do More with Your Time
Magnetic bead-based assays use Luminex® MagPlex magnetic microsphere. All other assay components are the same as the equivalent Luminex® polystyrene bead-based assays providing identical quality and consistency performance. MagPlex technology takes advantage of magnetic properties to simplify assay wash steps and maximize uniformity of results. Magnetic bead-based assays also enable automation, decrease hands-on time, and increase throughput and precision. Easy to use protocols allow you to get your results in as little as 3.5 hours. In addition, these assays are compatible with both vacuum and magnetic wash stations and work well with the MAGPIX® system as well as other Luminex® xMAP® platforms.

More Validation for Confidence in Your Results
Human and Monkey extracellular magnetic singleplex kits have been validated using the same rigorous criteria that distinguish Luminex® assays from other commercially available assays. With more than 20 years of experience, our fully qualified antibodies help ensure the specificity and sensitivity of our assays. Every singleplex kit and premixed multiplex kit comes with a product insert that outlines the specifications for each marker in the assay (see Documents section below).

Learn more about the complete Luminex® product line.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Stop Solution Invitrogen™

This stop solution is intended for use as a stop solution in enzyme assays which employ horseradish peroxidase and TMB substrate solution. It is suitable for use in microwell assays such as ELISA.

This product is For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

ProcartaPlex Streptavidin-PE Invitrogen™

The streptavidin fluorochrome conjugates are commonly used to detect biotinylated primary antibodies. Streptavidin binds to biotin with high affinity.

The ProcartaPlex Streptavidin-PE is included in our basic kits and off the shelf panels. Additionally, we offer the Streptavidin-PE as a stand-alone item.

Reported Application
Multiplex Immunoassay

Guardian™ Peroxidase Conjugate Stabilizer/Diluent Thermo Scientific™

Thermo Scientific Guardian Peroxidase Conjugate Stabilizer/Diluent preserves the functional integrity and activity of horseradish peroxidase conjugated antibodies and other proteins at very dilute concentrations for long-term storage.

Features of Guardian Peroxidase Conjugate Stabilizer/Diluent:

Preserves HRP activity—no significant loss of HRP activity over a six-month period at room temperature (1:1000 dilution) or 12 months at 4°C
Convenient—store ready-to-use dilutions (1:1000 to 1:100,000) that maintain enzyme activity in the refrigerator—no aliquoting or freezing
Assay compatible—simply add your favorite blocking buffer to create the ideal diluent for your HRP-based ELISA system or store the HRP conjugate as a 1:1000 stock solution for Western blots and dilute in the final assay buffer
Saves money—less expensive than competitor formulations or ordering new HRP conjugates

With Guardian Peroxidase Conjugate Stabilizer/Diluent, typical 1 mg/mL antibody or streptavidin peroxidase conjugates can be diluted as much as 100,000-fold for storage at 4°C. Most ELISA and blotting applications for HRP conjugates require dilution of typical 1 mg/mL stocks by at least 1000-fold (to 1 µg/mL). Sensitive assay systems, such as with chemiluminescent substrates, often require dilution up to 100,000-fold (10 ng/mL). The Guardian Solution enables these 1X working concentrations of HRP conjugate to be prepared in advanced and stored at 4°C for 12 months or at room temperature for six months.For blotting applications, it is usually best to prepare a concentration for storage that can be diluted 10-fold in assay buffer (which may contain blocking components) for final use.

Typical colorimetric ELISA procedures requires that 10 mL of HRP-conjugated secondary antibody be diluted 1000- to 5000-fold from a 1 mg/mL stock solution. That necessitates pipetting 2 to 10 µL of stock into a tube with 10 mL of diluent. More sensitive assay systems, such as Western blots with Thermo Scientific SuperSignal West Pico, Dura or Femto Chemiluminescent Substrates for HRP, require a second round of dilution to achieve the needed working concentration of HRP conjugate. With Guardian Stabilizer-Diluent, HRP conjugates can be pre-diluted and stored at concentrations that are either ready to use or easily diluted 10- to 100-fold for final use. Preparing larger volumes of pre-diluted stocks ensures consistency and greater accuracy for assays performed repeatedly day after day.

eBioscience™ ELISA Wash Buffer, 1L packets Invitrogen™

The eBioscience ELISA Wash Buffer Powder is a dried powder formulation of phosphate buffered saline with 0.05% Tween-20. Each packet is reconstituted in 1 Liter of distilled, deionized water; the resulting 1x PBS/Tween-20 buffer (pH 7.4) is suitable for washing ELISA plate microwells. 10 packets of ELISA Wash Buffer Powder are supplied, yielding a total of 10 liters of 1x Wash Buffer.

Reported Application
ELISA

ProcartaPlex Mouse High Sensitivity Basic Kit Invitrogen™

ProcartaPlex™ immunoassays are our ideal solution for assessing multiple protein biomarkers in a single sample.
Our ProcartaPlex assays use Luminex ™ xMAP technology for the multi-analyte detection of secreted proteins including cytokines, chemokines, growth factors and other proteins.
ProcartaPlex™ assays are subject to the same stringent and robust development and manufacturing as are our extensively validated Platinum and Instant ELISA kits. Quantitate multiple targets in cell/tissue culture supernatants, serum, and plasma.

Explore our broad portfolio of pre-configures panels and combinable single analytes.

For combination of only Simplex Kits an additional Basic Kit is required. This kit includes all non-target specific reagents - assay diluent, wash buffers, SA-PE, amplification reagents and plates.

Not intended for human or animal therapeutic or diagnostic use.

This kit does not contain a Multiscreen Filter Plate.

Suitable Sample Types
Cell culture supernatant, serum, plasma

Sample Volume
Serum, Plasma: 25 µL; CCS: 50 µL

Reported Application
Multiplex Immunoassay

Ethylene Glycol Thermo Scientific™

Thermo Scientific Pierce Ethylene Glycol Solution provides exceptional antifreeze protection and storage stability for antibody-enzyme conjugates because it is purified to remove impurities commonly found traditional glycerol stocks.

Features of Ethylene Glycol:

• Specially purified to remove impurities such as aldehydes, peroxides, iron and UV absorbing hydrocarbons
• Suitable for enzyme storage without the worry of losing enzymatic activity
• Stable for months

This product is a 50% (w/v) aqueous solution of highly purified ethylene glycol. When mixed in equal volume with purified protein samples, such as primary antibodies, the solution stabilizes and maintains the mixture as a liquid during freezer storage (-20°). Ethylene glycol is a suitable alternative to glycerol for most protein-storage applications. In fact, this preparation of ethylene glycol is devoid of various common glycerol impurities and potiential degradation products that are damaging to certain enzymes.

Assay Buffer (10X) Invitrogen™

This Assay Buffer is used in immunoassays and in Western and Southern blotting for alkaline phosphatase Tropix® substrate conjugates.

For Research Use Only. Not for use in diagnostics procedures.

HRP-Conjugate Diluent/Stabilizer Invitrogen™

This product may be used as a conjugate diluent in enzyme immunoassays.

Control Set for Human 42-Plex Human ProcartaPlex™ Panel 1, Platinum Invitrogen™

Platinum ProcartaPlex Human 42plex Control Set contains a high and low control for all analytes incuded into Platinum ProcartaPlex Human Panel 1 (42 plex). For list of analytes included into this set please refer to the TDS in the Documents Section.

Reactivity/Species
Human

Reported Application
Multiplex Immunoassay

Target List
IL-12; Brain-Derived Neurotrophic Factor (BDNF); CCL11 (Eotaxin); CCL3 (MIP-1 alpha); CCL4 (MIP-1 beta); CCL5 (RANTES); CD31 (PECAM-1); CD62P (P-Selectin); CXCL1 (GRO alpha); CXCL10 (IP-10); GM-CSF; HGF; IFN alpha; IFN gamma; IL-1 alpha; IL-1 beta; IL-1 Receptor Antagonist; IL-10; IL-13; IL-15; IL-16; IL-17A; IL-2; IL-20; IL-21; IL-4; IL-5; IL-6; IL-7; IL-8 (CXCL8); IL-9; LIF; OPG; sCD120b (sTNF Receptor II); Stem Cell Factor (SCF); TNF alpha; t-PA; TSLP; VEGF-A; VEGF-D; PDGF-BB; MCP-2

Bead Regions
See product documentation

Assay Range
See product documentation

Assay Sensitivity
See product documentation

Blocker™ BLOTTO in TBS Thermo Scientific™

Blocker BLOTTO Blocking Buffer is a ready-to-use 5% solution of nonfat powdered milk in Tris-buffered saline for western blot, ELISA, IHC, and other detection methods.

Compare and view all available blocking buffers

Features of Blocker BLOTTO Blocking Buffer:
Format—ready-to-use 1X TBS solution; can be diluted as needed
Formulation—formulated with anti-foaming agent and thimerosal-free preservative

Not recommended for avidin-based techniques because it contains some endogenous biotin.

SuperBlock™ (PBS) Blocking Buffer - Blotting Thermo Scientific™

Thermo Scientific SuperBlock (PBS) Blocking Buffer - Blotting is optimized PBS solution containing a protein that provides incredibly fast and effective blocking for Western blot analysis.

Features of SuperBlock (PBS) Blocking Buffer - Blotting:

Fast—block membranes in 5 to 10 minutes
Flexible—guaranteed to be biotin-free for use with streptavidin system
Low background—the non-serum protein solution yields a high signal-to-noise ratio
Stable—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes or tissues with a SuperBlock Buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

Related Products
SuperBlock™ (PBS) Blocking Buffer
SuperBlock™ T20 (PBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer
SuperBlock™ T20 (TBS) Blocking Buffer
SuperBlock™ (TBS) Blocking Buffer Dry Blend
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