Shop All General Buffers for Life Science

HEPES (1 M) (Gibco™)

Gibco® HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid) is a zwitterionic organic chemical buffering agent commonly used in cell culture media. The addition of 10–25 mM HEPES provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. HEPES is a good buffering choice for many cell culture systems because it is membrane impermeable, has limited effect on biochemical reactions, is chemically and enzymatically stable, and has very low visible and UV light absorbance.

Dual-site cGMP Manufacturing and Quality System
Gibco® HEPES is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® HEPES product made in our Grand Island facility (15630-080). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Endotoxin Binding Reagent (Thermo Scientific™)

Thermo Scientific GeneJET Endotoxin Binding Reagent is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 60 mL aliquots.

Precipitation Solution for MagJET Plant gDNA Kit (Thermo Scientific™)

Thermo Scientific MagJet Precipitation Solution is a component of the MagJET Plant Genomic DNA Kits (K2761/2762) and may be purchased separately. Provided in 16.5 mL aliquots.

TE Buffer (Invitrogen™)

This 1X TE Buffer is a component of the PureLink™ 96 Plasmid Purification System, now offered separately. It is used to resuspend the final purified plasmid pellet and contains very low EDTA, so it is compatible with sequencing and other enzymatic applications.

Composition:
10 mM Tris-HCl (pH 8.0)
0.1 mM EDTA

BupH™ MES Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of optimized amine-free and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions.

Features of BupH MES Buffered Saline Packs:

MES buffer—dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of MES buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7. MES is 2-(N-morpholino) ethanesulfonic acid.

Applications:
• Carboxyl-to-amine crosslinking procedures using carbodiimide crosslinker EDC (Part No. 22980)

Wash Solution I (concentrated) (Thermo Scientific™)

Thermo Scientific GeneJET Wash Solution I (concentrated) is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 360 mL aliquots.

Rebinding Buffer for MagJET Blood RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Rebinding Buffer (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately.

SequalPrep™ Wash Buffer (Applied Biosystems™)

For use with SequalPrep™ Normalization Plates, the wash buffer may be purchased separately.

Pierce™ 20X Modified Dulbecco's PBS Tween™ 20 Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is a space-saving stock solution used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays.

Features of 20X Modified Dulbecco's PBS Tween-20:

Dulbecco's PBS-T—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

Wash Buffer 1 for MagJET Viral Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Viral DNA and RNA Kit (K2782) and may be purchased separately. Provided in 90 mL aliquots.

TR-FRET Coregulator Buffer D

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

UltraPure™ 1M Tris-HCI, pH 8.0 (Invitrogen™)

UltraPure™ 1 M Tris-HCl Buffers are pre-mixed, pH-adjusted, sterile-filtered solutions. Prepared as 1 M concentrates, these buffers can be diluted to the desired concentration and used in molecular biology or general biochemistry applications.

Performance and Quality Testing: No DNase, RNase, or protease activity detected

Wash Solution I, RNA Purification (Invitrogen™)

This Wash Solution is for use with the Total RNA Chemistry System.

Total RNA Chemistry makes possible the rapid isolation of total RNA from various samples, including tissue-cultured cells, whole blood, and peripheral blood mononuclear cells (PBMCs). The protocol is designed for use with the 6100 Nucleic Acid PrepStation and 6700 Automated Nucleic Acid Workstation.

For Research Use Only. Not for use in diagnostics procedures.

Wash Buffer for Bisulfite Conversion Kit (concentrated) (Thermo Scientific™)

Thermo Scientific EpiJet Washing Buffer is a component of the EpiJET Bisulfite Conversion Kit and may be purchased separately. Provided in 9 mL aliquots.