Shop All General Buffers for Life Science

Pierce™ 20X Modified Dulbecco's PBS Tween™ 20 Buffer Thermo Scientific™

Thermo Scientific Pierce 20X Modified Dulbecco's PBS Tween-20 is a space-saving stock solution used to prepare physiological Na- and K-phosphate buffered saline (D-PBS) wash and blocking buffers for ELISA, Western and other immunoassays.

Features of 20X Modified Dulbecco's PBS Tween-20:

Dulbecco's PBS-T—diluted 20-fold in water, the solution yields 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween-20, pH 7.4
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Modified Dulbecco's PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14M NaCl, 10 mM KCl, 0.05% Tween™ 20, pH 7.4, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Modified Dulbecco's PBS Buffer

Pierce™ 20X Phosphate Buffered Saline Thermo Scientific™

Thermo Scientific Pierce 20X Phosphate Buffered Saline is a space-saving stock solution that is ideal for preparing PBS buffers for use in crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer.

Features of 20X Phosphate Buffered Saline:

PBS—diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15M NaCl, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X Phosphate Buffered Saline (PBS) makes 10 mM sodium phosphate, 0.15M NaCl, pH 7.5, when diluted to 1X with water.

Applications:
• Crosslinking, biotinylation and fluorescent labeling reactions requiring an amine-free buffer

Related Products
BupH™ Phosphate Buffered Saline Packs

FRAP1 (mTOR) Assay Buffer

1X Assay Buffer for use with the LanthaScreen® FRAP1 (mTOR) assays.

RNA Fragmentation Reagents Invitrogen™

For the fragmentation of aRNA prior to hybridization with oligonucleotide arrays. The protocol is simple, fast and can be completed in less than 20 min. Sufficient Ambion® reagents are included for 200 rxns. Many protocols for using amplified RNA in microarray analysis recommend fragmenting the aRNA prior to hybridization with an oligonucleotide microarray. This fragmentation step improves hybridization kinetics with the arrayed oligonucleotides and can lead to enhanced signal. The protocol is simple. Mix the aRNA with the Ambion® 10X RNA Fragmentation Reagent and heat to 70°C for 15 min. The rxn is then stopped by the addition of the Stop Solution and placed on ice until ready for hybridization with the microarray. The average size of the resulting fragments will be 60-200 nucleotides. Up to 10 µg of RNA can be fragmented in a 10 µL rxn.

Rebinding Buffer for MagJET Blood RNA Kit (concentrated) Thermo Scientific™

Thermo Scientific MagJet Rebinding Buffer (conc.) is a component of the MagJET Whole Blood RNA Kit (K2751) and may be purchased separately.

ChargeSwitch™ Wash Buffer W12 Invitrogen™

ChargeSwitch™ W12 Buffer is a wash buffer used in all ChargeSwitch kits including the ChargeSwitch magnetic beads and ChargeSwitch-Pro column kits.

Solution D

Solution D is an anion transport inhibitor, for cells such as CHO-K1, that can be used in conjunction with CCF2-AM and CCF4-AM-based studies to prevent active cellular export of the FRET-based substrates after loading.

UltraPure™ SSPE, 20X Invitrogen™

UltraPure™ 20X SSPE is a prepared buffer concentrate formulated for use in nucleic acid hybridizations and blot transfer applications. Supplied in 1 L plastic bottles, or in a 4 L or 10 L stackable Cubitainer¤ Box, sterile-filtered UltraPure™ 20X SSPE contains 3.0 M NaCl, 0.2 M NaH2PO4, and 0.02 M EDTA at pH 7.4.

Performance and Quality Testing: No DNase or RNase activity detected.

Pierce™ 20X PBS Tween™ 20 Buffer Thermo Scientific™

Thermo Scientific Pierce 20X PBS Tween-20 is a space-saving stock solution that is ideal for preparing PBS-Tween (PBS-T) wash buffers for ELISA, Western and other immunoassays as well as a blocking buffer for plate-based assays.

Features of 20X PBS Tween-20:

PBS-T—diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween-20, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween™ 20, pH 7.5, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

Related Products
Pierce™ 20X Phosphate Buffered Saline

Neutralization Solution for MagJET Plasmid DNA Kit Thermo Scientific™

Thermo Scientific MagJet Neutralization Solution is a component of the MagJET Plasmid DNA Kits (K2791/2792) and may be purchased separately. Provided in 25 mL aliquots.

BupH™ Borate Buffer Packs Thermo Scientific™

Thermo Scientific BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of borate buffer for use in protein modification procedures requiring amine-free conditions and alkaline pH.

Features of BupH Borate Buffer Packs:

Borate buffer—dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of borate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5.

Applications:
• Protein modification procedures requiring amine-free buffer at alkaline pH

Related Products
Pierce™ 20X Borate Buffer

Pierce™ 20X TBS Tween™ 20 Buffer Thermo Scientific™

Thermo Scientific Pierce 20X TBS Tween-20 is a space-saving stock solution that is ideal for preparing TBS-Tween (TBS-T) wash buffers or blocking buffer diluent used for applications such as ELISA, Western blotting and other immunoassays.

Features of Thermo Scientific Pierce 20X TBS Tween-20:

TBS-T—diluted 20-fold in water, the solution yields 25 mM Tris, 0.15M NaCl, 0.05% Tween-20, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Applications:
• Wash buffer or blocking buffer for ELISA, Western and other immunoassays

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) makes 25 mM Tris, 0.15M NaCl, 0.05% Tween-20, pH 7.5, when diluted to 1X with water.

Related Products
Pierce™ 20X TBS Buffer

Wash Buffer for Bisulfite Conversion Kit (concentrated) Thermo Scientific™

Thermo Scientific EpiJet Washing Buffer is a component of the EpiJET Bisulfite Conversion Kit and may be purchased separately. Provided in 9 mL aliquots.

Wash Buffer for GeneJET Gel Extraction Kit Thermo Scientific™

Thermo Scientific GeneJET Wash Buffer is a component of the GeneJET Gel Extraction Kit (K0691/K0692) and may be purchased separately. Provided in 45 mL aliquots.
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