Shop All General Buffers for Life Science

TR-FRET Coregulator Buffer D

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

Resuspension Solution for GeneJET Plasmid Maxiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Resuspension Buffer is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately.

Neutralization Solution (Thermo Scientific™)

Thermo Scientific GeneJET Neutralization Solution is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 240 mL aliquots.

Elution Buffer for GeneJET Plasmid Midiprep Kit (Thermo Scientific™)

Thermo Scientific GeneJET Elution Buffer is a component of the GeneJET Plasmid Midiprep Kit and may be purchased separately. Provided in 60 mL aliquots.

TE Buffer (20X), RNase-free (Invitrogen™)

The RNase-free TE buffer concentrate, 20X TE buffer, which is essential to the success of the RiboGreen® RNA quantitation assays (R11491, R37302) is available separately from the kits to extend the number of low-concentration assays.

Antibody Diluent

Ready-to-use diluent with BSA (for stabilizing the antibody) and preservative.

Modification Solution I (Thermo Scientific™)

Thermo Scientific EpiJet Modification Solution II is a component of the EpiJET Bisulfite Conversion Kit and may be purchased separately. Provided in 0.35 mL aliquots.

FRAP1 (mTOR) Assay Buffer

1X Assay Buffer for use with the LanthaScreen® FRAP1 (mTOR) assays.

PureLink™ PCR Purification Binding Buffer B2 (Invitrogen™)

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit. It is added directly to PCR products in the first step of the PureLink™ PCR Purification Kit procedure to facilitate DNA binding to the silica membrane. The buffer is activated by the addition of 80mls of Isopropanol.

The PureLink™ PCR Purification Kit is used for purifying PCR products ranging in size from 100 bp-12kb.

Endotoxin Binding Reagent (Thermo Scientific™)

Thermo Scientific GeneJET Endotoxin Binding Reagent is a component of the GeneJET Plasmid Maxiprep Kit (K0491/K0492) and may be purchased separately. Provided in 60 mL aliquots.

Plant RNA Lysis Solution (Thermo Scientific™)

Thermo Scientific GeneJET Plant RNA Lysis Solution is a component of the GeneJET Plant RNA Purification Mini Kit (K0801/K0802) and may be purchased separately. Provided in 200 mL aliquots.

Wash Buffer 1 for MagJET Plant RNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Wash Buffer 1 (conc.) is a component of the MagJET Plant RNA DNA Kits (K2771/2772) and may be purchased separately. Provided in 110 mL aliquots.

Kinase Buffer T (5X)

Kinase buffer that has been specially optimized for use with the Type III PI4 kinases including PI4KA and PI4KB.

Lysis Buffer for MagJET RNA Kit (Thermo Scientific™)

Thermo Scientific MagJET Lysis Buffer is a component of the MagJET RNA Kit (K2731/2732) and may be purchased separately.

Pierce™ 20X PBS Tween™ 20 Buffer (Thermo Scientific™)

Thermo Scientific Pierce 20X PBS Tween-20 is a space-saving stock solution that is ideal for preparing PBS-Tween (PBS-T) wash buffers for ELISA, Western and other immunoassays as well as a blocking buffer for plate-based assays.

Features of 20X PBS Tween-20:

PBS-T—diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween-20, pH 7.5
Easy to use—no packets to open and no powder to dissolve
Increased accuracy—eliminates the possibility of powder remaining in a packet
Saves time—20X concentration eliminates time spent waiting for powder to dissolve
Saves space—storage as concentrated stock minimizes bench space needed for solutions

Pierce Concentrated Buffers are ready to use without having to weigh and dissolve dry ingredients or to adjust the pH with concentrated acid or base. Simply dilute the stock solution with pure water and proceed with your experiment. The 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15M NaCl, 0.05% Tween™ 20, pH 7.5, when diluted to 1X with water.

Applications:
• A wash buffer for ELISA, Western blotting and other immunoassays
• Diluent or blocking buffer for plate-based assays

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