Shop All General Buffers for Life Science

Binding Buffer for MagJET Blood gDNA Kit (concentrated) (Thermo Scientific™)

Thermo Scientific MagJet Binding Buffer (conc.) is a component of the MagJET Whole Blood gDNA Kits (K2741, K2742) and may be purchased separately.

Kinase Quench Buffer

Kinase Quench Buffer (500 mM EDTA) is an essential component for kinase assays using LanthaScreen® technology. The Kinase Quench Buffer is used to stop the kinase reaction.

BupH™ Borate Buffer Packs (Thermo Scientific™)

Thermo Scientific BupH Borate Buffer Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of borate buffer for use in protein modification procedures requiring amine-free conditions and alkaline pH.

Features of BupH Borate Buffer Packs:

Borate buffer—dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of borate buffer are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 50 mM borate, pH 8.5.

Applications:
• Protein modification procedures requiring amine-free buffer at alkaline pH

Related Products
Pierce™ 20X Borate Buffer

MagMAX™ Wash Solution 2 Concentrate (Invitrogen™)

Ambion® MagMAX™ Wash Solution 2 Concentrate is a component of all the MagMAX™ Isolation Kits that may be purchased separately. Provided in one 200 mL bottle.

Lysis Solution (Thermo Scientific™)

Thermo Scientific GeneJET Lysis Solution is a component of several GeneJET nucleic acid purification kits and may be purchased separately. Please consult kit protocols to confirm catalog numbers for re-ordering the appropriate kit-specific components.

ResDNASEQ™ Dilution Buffer (Applied Biosystems™)

ResDNASEQ Dilution Buffer, a component of the ResDNASEQ Residual DNA Quantitation kits, is offered separately here for those kit users who need additional elution buffer. As with other products in the Pharma Analytics Sample Preparation product line, ResDNASEQ Dilution Buffer:
• Is completely supported by our global manufacturing and distribution centers
• Has a Certificate of Analysis available
• Has a shelf-life of up to 12 months

TR-FRET Coregulator Buffer B

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

Loading Solutions

This kit contains the solutions needed to conveniently load the FRET-based, beta-lactamase substrates, CCF2-AM and CCF4-AM, into cells.

RNA Fragmentation Reagents (Invitrogen™)

For the fragmentation of aRNA prior to hybridization with oligonucleotide arrays. The protocol is simple, fast and can be completed in less than 20 min. Sufficient Ambion® reagents are included for 200 rxns. Many protocols for using amplified RNA in microarray analysis recommend fragmenting the aRNA prior to hybridization with an oligonucleotide microarray. This fragmentation step improves hybridization kinetics with the arrayed oligonucleotides and can lead to enhanced signal. The protocol is simple. Mix the aRNA with the Ambion® 10X RNA Fragmentation Reagent and heat to 70°C for 15 min. The rxn is then stopped by the addition of the Stop Solution and placed on ice until ready for hybridization with the microarray. The average size of the resulting fragments will be 60-200 nucleotides. Up to 10 µg of RNA can be fragmented in a 10 µL rxn.

UltraPure™ 1 M Tris-HCI Buffer, pH 7.5 (Invitrogen™)

UltraPure™ 1 M Tris-HCl Buffer, pH 7.5, is a premixed, pH-adjusted, sterile-filtered solution. Prepared as a 1 M concentrate, this buffer can be diluted to the desired concentration and used in molecular biology or general biochemistry applications.

Performance and quality testing
No DNase, RNase, or protease activity detected.

BupH™ MES Buffered Saline Packs (Thermo Scientific™)

Thermo Scientific BupH MES Buffered Saline Packs are pouches of dry-blended powder that are each sufficient to make 500 mL of optimized amine-free and carboxyl-free buffer for carbodiimide (EDC) crosslinking and conjugation reactions.

Features of BupH MES Buffered Saline Packs:

MES buffer—dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7
Convenient—dissolve contents of one envelope in water and the buffer is ready to use
Save time and trouble—no weighing, no pH adjustment, no need to stock individual components, and no need to make and store large volumes of stock solution in advance of daily needs
Long shelf life—stocking and storage as dry packs eliminates concerns about long-term stability of stock solutions
Eliminate variables—our quality control ensures that every pack will yield the same, consistent buffer

BupH Dry-Blend Packs of MES buffered saline are easy to use. Simply empty contents of one foil envelope pack into a beaker, add ultrapure water and stir to dissolve. The packs eliminate weighing time and tedious pH adjustments. When dissolved in 500 mL of water, each pack makes 0.1M MES, 0.9% sodium chloride, pH 4.7. MES is 2-(N-morpholino) ethanesulfonic acid.

Applications:
• Carboxyl-to-amine crosslinking procedures using carbodiimide crosslinker EDC (Part No. 22980)

TR-FRET Coregulator Buffer G

A specialized nuclear receptor buffer that can be used with Invitrogen's collection of purified nuclear receptor proteins and assays.

SSC (20X), RNase-free (Invitrogen™)

Molecular biology grade, Ambion® 20X SSC solution is supplied in four bottles containing 1 L each. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.

Tris (1 M), pH 7.0, RNase-free (Invitrogen™)

Ambion®  Molecular biology grade, 1 M Tris, pH 7.0 solution is supplied in one bottle containing 100 mL. The buffer is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging. Ambion®'s nuclease-free reagents and buffers are manufactured in facilities specifically designed to prevent the introduction of nucleases. Highly sensitive RNase assays are performed at several different stages of the manufacturing process to ensure the highest quality. These reagents are rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.