Shop All Microscopy Reagents

SlowFade™ Gold Antifade Mountant with DAPI (Invitrogen™)

SlowFade® Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade® Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

SlowFade® Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, SlowFade® Diamond Antifade Mountant is recommended. Learn more about key differences between SlowFade® Gold and SlowFade® Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor® dyes

Get Photobleaching Protection Without Quenching

The antifade reagents used in some mountants can destroy the initial strength of many fluorophores (especially those that emit above 550 nm) by up to 80%. To create the Molecular Probes® Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Immediate Viewing with Maximum Signal Protection
While epoxy-based mountants used in our FluoCells® series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. SlowFade® Gold Antifade Mountant is the best option for immediate viewing of samples. For longer-term storage, it is recommended that a sealant (i.e., finger nail polish or equivalent acrylic reagent) be applied to the coverslip edges to prevent drying. Alternatively, choose our curing mountant ProLong® Gold Antifade Mountant.

Improved Resolution in Thick Sections
Glycerol-based mountants are recommended when viewing thick samples, as the refractive index of glycerol is closer to that of tissue. It's easy to use—just air-dry your stained samples to remove excess water and mount.

ProLong™ Live Antifade Reagent, for live cell imaging (Invitrogen™)

ProLong® Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging. When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong® Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong® Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours.

Features of ProLong® Live Antifade Reagent include:
• Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker®, and CellTracker®dyes
• Little-to-no effect on cellular viability, proliferation, or other cellular functions
• Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image

ProLong® Live Antifade Reagent contains enzymes from the plasma membrane of naturally occurring E. coli. These enzymes metabolize the elements that can cause photobleaching. They work in the extracellular space and are not known to enter cells, so intracellular functions are minimally affected by ProLong® Live Antifade Reagent. As shown in figure below, cells grown in the presence of ProLong® Live Antifade Reagent display minimal effects on cellular viability or proliferation. ProLong® Live Antifade Reagent provides photobleach protection for most fluorophores frequently used in live cell fluorescent imaging, such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker® and CellTracker® dyes. ProLong® Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.

SlowFade™ Gold Antifade Mountant

SlowFade® Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade® Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

SlowFade® Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, SlowFade® Diamond Antifade Mountant is recommended. Learn more about key differences between SlowFade® Gold and SlowFade® Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor® dyes

Get Photobleaching Protection Without Quenching

The antifade reagents used in some mountants can destroy the initial strength of many fluorophores (especially those that emit above 550 nm) by up to 80%. To create the Molecular Probes® Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Immediate Viewing with Maximum Signal Protection
While epoxy-based mountants used in our FluoCells® series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. SlowFade® Gold Antifade Mountant is the best option for immediate viewing of samples. For longer-term storage, it is recommended that a sealant (i.e., finger nail polish or equivalent acrylic reagent) be applied to the coverslip edges to prevent drying. Alternatively, choose our curing mountant ProLong® Gold Antifade Mountant.

Improved Resolution in Thick Sections
Glycerol-based mountants are recommended when viewing thick samples, as the refractive index of glycerol is closer to that of tissue. It's easy to use—just air-dry your stained samples to remove excess water and mount.

ProLong™ Live Antifade Reagent, for live cell imaging (Invitrogen™)

ProLong® Live Antifade Reagent helps prevent the loss of fluorescent signal due to photobleaching during live cell imaging. When dyes are illuminated, they can degrade (usually permanently), reducing the sample’s fluorescent intensity and leading to the creation of free radical singlet oxygen, an element that can further degrade neighboring dye molecules. ProLong® Live Antifade Reagent metabolizes this element, increasing the signal intensity and duration while keeping the background signal low. ProLong® Live reagent has little-to-no effect on cellular viability, proliferation, or other cellular functions in experiments up to 48 hours.

Features of ProLong® Live Antifade Reagent include:
• Photobleach protection in live cell experiments, for fluorophores such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker®, and CellTracker®dyes
• Little-to-no effect on cellular viability, proliferation, or other cellular functions
• Easy to use, just add to the imaging solution or complete cell culture media or buffer, incubate, and image

ProLong® Live Antifade Reagent contains enzymes from the plasma membrane of naturally occurring E. coli. These enzymes metabolize the elements that can cause photobleaching. They work in the extracellular space and are not known to enter cells, so intracellular functions are minimally affected by ProLong® Live Antifade Reagent. As shown in figure below, cells grown in the presence of ProLong® Live Antifade Reagent display minimal effects on cellular viability or proliferation. ProLong® Live Antifade Reagent provides photobleach protection for most fluorophores frequently used in live cell fluorescent imaging, such as GFP, RFP, Hoechst 33342, MitoTracker®, LysoTracker® and CellTracker® dyes. ProLong® Live Antifade Reagent can be added to any cell culture media or buffer that is suitable for fluorescent imaging.

CytoVista™ 3D Cell Culture Clearing/Staining Kit (Invitrogen™)

The CytoVista 3D Cell Culture Clearing/Staining Kit clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm. The kit includes CytoVista 3D Cell Culture Clearing Reagent, penetration buffer, wash buffer, blocking buffer, and antibody dilution buffer.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

CytoVista™ 10X Wash Buffer (Invitrogen™)

CytoVista 10X Wash Buffer is used in wash steps when performing immunohistochemistry (IHC) or immuno-labeling with tissue or 3D cell culture. This buffer is 10X concentrated and should be diluted to 1X with PBS before use. It consists of PBS with 0.2% Tween-20 and 10 µg/mL heparin and helps enable sharp and bright images at any depth of tissue or 3D cell culture when used with other CytoVista products.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

CytoVista™ 3D Cell Culture Clearing Reagent (Invitrogen™)

CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm. The reagent can also be used as an imaging solution with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology or brightness, or specificity of the fluorophores.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

SlowFade™ Gold Antifade Mountant with DAPI

SlowFade® Gold Antifade Mountant is a liquid mountant applied directly to fluorescently labeled cell or tissue samples on microscope slides. It contains chemical components designed to protect fluorescent dyes from fading (photobleaching) during fluorescence microscopy experiments. SlowFade® Gold Antifade Mountant is glycerol-based, with no mixing or curing required, which makes it ideal for immediate viewing of the sample. It comes ready-to-use—just apply a drop to the sample, add a coverslip, and image. It is available with or without DAPI nuclear stain.

SlowFade® Gold Antifade Mountant is not recommended for mounting samples containing fluorescent proteins, such as GFP. For superior antifade protection of fluorescent proteins and fluorescent dyes, SlowFade® Diamond Antifade Mountant is recommended. Learn more about key differences between SlowFade® Gold and SlowFade® Diamond antifade mountants.

Key Attributes:

• Protects dyes from fading during imaging
• Ready-to-use liquid, ideal for immediate sample viewing
• Mounted samples are stable for weeks
• Maintains signal strength—little to no quenching
• Ideal for Alexa Fluor® dyes

Get Photobleaching Protection Without Quenching

The antifade reagents used in some mountants can destroy the initial strength of many fluorophores (especially those that emit above 550 nm) by up to 80%. To create the Molecular Probes® Gold series of mountants, our scientists developed a formula of chemical additives that minimize this initial signal quenching for the broadest range of colors and dyes.

Immediate Viewing with Maximum Signal Protection
While epoxy-based mountants used in our FluoCells® series of prepared cells can be stable for years and afford the highest level of antifade protection, they are difficult to use, requiring a series of steps in organic solvents prior to mounting. SlowFade® Gold Antifade Mountant is the best option for immediate viewing of samples. For longer-term storage, it is recommended that a sealant (i.e., finger nail polish or equivalent acrylic reagent) be applied to the coverslip edges to prevent drying. Alternatively, choose our curing mountant ProLong® Gold Antifade Mountant.

Improved Resolution in Thick Sections
Glycerol-based mountants are recommended when viewing thick samples, as the refractive index of glycerol is closer to that of tissue. It's easy to use—just air-dry your stained samples to remove excess water and mount.

Citrate Buffer (pH 6.0), Concentrate

Citrate buffer is used in Heat Induced Epitope Retrieval (HIER) methods. HIER is used to reverse the loss of antigenicity that occurs with some epitopes in formalin-fixed paraffin embedded tissues. During epitope retrieval, tissue slides are immersed in a solution and heated (>95° C) by hot plate or other appropriate heat source such as a pressure cooker or steamer. Citrate buffer is the preferred solution for most antibodies.

Avidin/Biotin Blocking Kit

This kit is used to block endogenous biotin or biotin-binding activity in tissue sections. The kit contains 18 mL each of Avidin and Biotin solutions.

CytoVista™ 2-mm Coronal Mouse Brain Slicer (Invitrogen™)

This 2-mm coronal mouse brain slicer allows you to easily cut a mouse brain into smaller pieces for easier imaging with objectives that have a limited working distance. This slicer is 3D-printed out of resin and can be easily washed and cleaned between uses. All you need is a standard razor blade to start slicing your tissues.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

QuantiGene™ Sample Processing Kit, FFPE samples (Invitrogen™)

The QuantiGene Sample Processing Kit for formalin-fixed paraffin-embedded (FFPE) samples contains reagents and instructions for the preparation of tissue homogenates from FFPE tissue samples for use in QuantiGene Singleplex and QuantiGene Plex assays for RNA or DNA targets. H&E stained slides are compatible with QuantiGene assays.

• Easy to follow instructions
• Preserves the integrity of the RNA/DNA without degradation
• Choose the method that matches your FFPE tissue sample

What you get
The QuantiGene Sample Processing Kit for FFPE samples contains our proprietary homogenizing solution, proteinase K, and detailed instructions for preparing tissue homogenates from FFPE tissue sections.

ProLong™ Glass Antifade Mountant (Invitrogen™)

ProLong Glass Antifade Mountant is a glycerol-based, hard-setting, ready-to-use mountant that can be applied directly to fluorescently labeled cells or tissue samples on microscope slides or coverslips. Prolong Glass mountant has a refractive index of 1.52 after curing, similar to that of glass coverslips, compatible immersion oil, and oil-immersion microscope optics, enabling superior resolution and sensitivity. ProLong Glass mountant is also designed to provide unparalleled photobleach protection across the visible and near infra-red spectra. You can use ProLong Glass Antifade Mountant with almost any fluorescent dye or fluorescent protein (e.g., GFP, RFP, mCherry) and with any cell/tissue type for image depths ranging from 0 to 100 µm for bright, high-resolution Z-Stack, 3D, and 2D images.

With its high refractive index of 1.52 and superior antifade formulation, ProLong Glass Antifade Mountant delivers the following benefits:
• Up to 75% improvement in axial resolution for sharp images
• 3–4 times more imageable focal depth compared tomountants with refractive index of 1.47, with sharp images for monolayers of cells, tissue slices, or 3D cell cultures up to 150 μm thickness/focal depth
• Low background across the spectrum
• Preserved signals from inhibited photobleaching
• Weeks to months of stability for slide-mounted samples
• Ease of use
• Availability with or without DAPI

Learn more about ProLong Antifade mountants >

ProLong GlassAntifade Mountant comes already mixed and ready to use in a dropper bottle or 10-mL bottle with or without NucBlue (Hoechst 33342) stain, a cell-permeant nuclear counterstain that emits blue fluorescence when bound to DNA. After removing excess water, just apply a drop of mountant to the sample, add a coverslip, cure, and image. ProLong Glass mountant is a hard-setting, curing mountant that minimizes optical refraction and spherical aberration in the optical path, enables longer-term storage of your samples, and helps protect your samples from photobleaching. ProLong Glass mountant does not discolor or shrink when cured, making it possible to take high-quality fluorescent images weeks, or even months, after mounting the slides.

ProLong Glass Antifade Mountant with NucBlue stain contains an optimized concentration of Hoechst 33342 stain, which provides superior nuclear labeling of most cells and tissues. ProLong Glass mountant with NucBlue stain is not recommended for specimens thicker than 30 µm. Thicker specimens can require longer incubations with labeling reagents, so a pre-incubation with NucBlue Live ReadyProbes Reagent is recomended.

Brightest and sharpest images
Studies show that the axial resolution of a microscopy system can be improved by matching the refractive index of the mounting medium, which maximizes light collection of the objective lens (1–3). Fouquet et. al. saw dramatic improvement in axial resolution while using a confocal microscope with high refractive index media (3). When Prolong Glass Antifade Mountant, which has a refractive index of 1.52, is compared with a mounting medium with a refractive index of 1.47, the axial resolution is improved by 32% at a focal depth of 40 µm and 75% at a focal depth of 100 µm (see figure below). The signal intensity and sensitivity of fluorophores is also improved as the focal depth increases within the sample. The same or greater improvement in resolution and sensitivity is seen in the lateral direction. This makes Prolong Glass a superior mountant for acquisition of Z-Stack, 3D, and 2D images of cells and tissue at depths from 0 to 100 µm (see figure below).

Inhibit photobleaching across the visible and near-IR spectra
No matter which fluorescent dyes or fluorescent proteins you are using, ProLong Glass Antifade Mountant helps provide superior protection against photobleaching. It has been shown to have best-in-class performance with a wide array of fluorescent dyes, including Alexa Fluor, FITC, TRTC, Texas Red, Cy3, and Cy5 dyes (see figure below). ProLong Glass mountant does not quench fluorophores, and its ability to provide brighter signal against no background helps ensure that you get the best-quality high-resolution images for publishing.

Stable signals during imaging and for longer-term storage
ProLong Glass Antifade Mountant is the ideal option for archiving samples with fluorescent dyes or fluorescent proteins and for preserving signal strength during prolonged imaging sessions, such as repeated scanning under confocal laser scanning illumination or with super-resolution microscopy. And it's easy to use—simply remove excess water from the stained sample and mount. For immediate viewing of the sample, choose our non-curing mountant: SlowFade Diamond Antifade Mountant.

Achieve excellent refractive index matching for crisp images
For modern high-resolution imaging applications, refractive index matching is critical. ProLong Glass Antifade Mountant cures to a refractive index of 1.52. Additionally, this curing property eliminates the need to use messy acrylic resins (i.e., fingernail polish), which can damage or ruin modern microscope objectives.

References
1: Cremer C, Masters BR. Resolution enhancement techniques in microscopy. Eur Phys J. 2013 38(3):281-344.
2: Shimi T, Kittisopikul M, Tran J, Goldman AE, Adam SA, Zheng Y, Jaqaman K, Goldman RD. Structural organization of nuclear lamins A, C, B1, and B2 revealed by superresolution microscopy. Mol Biol Cell. 2015 26(22):4075-86.
3: Fouquet C, Gilles JF, Heck N, Dos Santos M, Schwartzmann R, Cannaya V, Morel MP, Davidson RS, Trembleau A, Bolte S. Improving axial resolution in confocal microscopy with new high refractive index mounting media. PLoS One. 2015 10(3):e0121096.

CytoVista™ 3D Cell Culture Clearing Reagent (Invitrogen™)

CytoVista 3D Cell Culture Clearing Reagent clears fluorescently labeled 3D cultured cells such as organoids and spheroids. This clearing process enables sharp and bright 3D fluorescent imaging for depths up to 1000 µm. The reagent can also be used as an imaging solution with stable storage of 3D cell cultures for weeks with minimal change in tissue morphology or brightness, or specificity of the fluorophores.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.

CytoVista™ Permeabilization Buffer (Invitrogen™)

CytoVista Permeabilization Buffer is used while performing immunohistochemistry (IHC) or immuno-labeling with tissue. This buffer is highly recommended for larger, more collagenous, and difficult-to-clear tissues (e.g., liver tissue, archived (FFPE) tissue). It helps reagents penetrate thick tissue, helping enable sharp and bright images at any tissue depth when used with other CytoVista products.

Learn more about CytoVista 3D Culture Clearing reagents ›

Features of the CytoVista family of tissue and 3D cell culture clearing reagents include:
• Rapid clearing of fluorescently labeled tissue for 3D imaging
• Minimal shrinkage, expansion, or other morphological changes to tissue
• Simple workflow does not require any special instruments or equipment and is easily adapted to IHC
• Compatible with most fluorophores, including dyes and fluorescent proteins

The CytoVista tissue and 3D cell culture clearing workflow clears tissue or 3D cultured cells with minimal changes to morphology or the sensitivity of detection with almost any fluorophore. With an easy-to-use protocol, whole mouse brain (8-mm section) can be cleared in 24 hours, or a 1-mm section in 2 hours, without the use of any special instruments or equipment.

The CytoVista clearing workflow is compatible with most fluorophores, including fluorescent proteins that are detected with common fluorescent imaging instruments, such as wide-field microscopes, high content analyzers, confocal microscopes, and lightsheet microscopes. For first time users, we recommend the CytoVista Tissue Clearing/Staining Kit (Cat No V11324) or CytoVista 3D Cell Culture Clearing/Staining Kit (Cat No. V11325). These kits contain all necessary reagents for both clearing and IHC staining.

CytoVista clearing technology sufficiently clears tissue for 3D fluorescent imaging, but unlike other clearing techniques does not significantly changes the tissue morphology. Minimal morphological changes, such as shrinkage and contraction, have been observed. For precious samples, the clearing can be reversed, and the tissue can be processed for histology studies, like H&E staining.

For tissues that are easier to clear, like brain, lung, intestine, skin, and tissues of less than 250-µm thickness, treatment with CytoVista Tissue Clearing Reagent is sufficient for 3D imaging. However, tissues of more than 250-µm thickness or that are hard to clear like kidney, liver, heart, and placenta will require an initial treatment with CytoVista Tissue Clearing Reagent followed by treatment with CytoVista Tissue Clearing Enhancer.

CytoVista 3D Cell Culture Clearing Reagent is recommended for spheroids or organoids up to 1 mm in thickness.

For hard tissues like bone, cartilage, and connective tissue, CytoVista Tissue Clearing Reagent is not recommended.