Shop All TR-FRET Primary Antibodies

LanthaScreen™ Tb-anti-AKT [pSer473] Antibody

AKT is phosphorylated at Ser473 by the rapamycin-insensitive mTor complex (mTORC2).
For use in combination with the LanthaScreen® AKT HEK293E cell line (K1516) to evaluate inhibitors in medium to high throughput screening applications.

LanthaScreen™ Tb-PY20 Antibody Kit

A general reagent to monitor tyrosine kinase activity, the pY20 antibody binds to phosphorylated tyrosines within a peptide or physiological protein substrate.

For use in combination with Fluorescein-poly-GT (PV3610) or Fluorescein-poly-GAT (PV3611) to evaluate inhibitors in medium to high throughput screening applications.

LanthaScreen™ Tb-pSer (PKC) Antibody Kit

A general assay for the Protein Kinase C (PKC) family, the antibody detects the phosphorylation of the serine within the PKC substrate.

For use in combination with the Fluorescein-PKC substrate (PV3506) to evaluate inhibitors in medium to high throughput screening applications.

LanthaScreen™ Tb-anti-LRRK2 [pSer935] Antibody

The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody enables researchers to interrogate phosphorylation at serine 935 of the Leucine-Rich Repeat Kinase-2 (LRRK2) protein in a cellular, high-throughput screening (HTS)-compatible, and TR-FRET format.

This antibody has been specifically developed and optimized for use in our LanthaScreen® TR-FRET LRRK2 Cellular Activity Assay Kits (available for both wildtype and disease relevant mutant protein), which utilize the BacMam gene delivery system. The antibody, as well as other components of these assay kits, are also available separately. This assay format provides a convenient tool for the expression of LRRK2-GFP fusion protein in the cell background of your choice. The resulting cellular assay can be used to identify LRRK2 and relevant mutant inhibitor.

This product includes:

• LanthaScreen® Tb-anti-LRRK2 [pSer935] Antibody, 1 mg (enough to perform 275 384-well plate assays)

Additional products needed for cell-based assay:

BacMam LRRK2-GFP Wild Type Reagent, 15 mL or BacMam LRRK2-GFP G2019S Reagent, 15 mL
LanthaScreen® 6x Lysis Buffer, 50 mL
Instrument Control Terbium TR-FRET Kit

Benefits of this assay include:

More Physiologically Relevant: cell-based LRRK2 kinase activity and choice of cell background
More Convenient: compatible with High Throughput Screening (HTS); LanthaScreen® technology reduces interference and noise
Disease Relevant: screen compound libraries for Parkinson's disease-relevant molecules

More Physiologically Relevant
The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody allows the investigation of phosphorylation at serine 935 on LRRK2 protein kinase. When paired with LRRK2 BacMam reagents, you have the freedom to choose the cellular background for your assay, including primary cells. This enables screening for potential inhibitors of LRRK2 proteins in their natural complexes in a physiologically relevant cell type.

More Convenient
Assays are run in a fully homogenous, addition-only format without any of the washing, lysate transfer, or separation procedures required for traditional methods such as western blotting and ELISA, making this assay ideal for HTS applications. The LanthaScreen® technology provides all of the advantages of TR-FRET ratiometric detection, including reduced data noise, less interference from fluorescent compounds, and high sensitivity, which results in the use of fewer cells than traditional Western or ELISA methods.

Disease Relevant
The LanthaScreen® Tb-Anti-LRRK2 [pSer935] Antibody can be used by Parkinson's and other disease researchers to perform HTS, cell-based assays to generate inhibition curves (IC50) for their compound libraries. This will aid drug discovery efforts by reducing the time to results from 4 hrs to typically 1.5 hrs compared to alternative ELISAs.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Related Links:
LRRK2 tools for advancing Parkinson's disease research
Kinase protein portfolio
LanthaScreen® Eu Kinase Binding Assay
LanthaScreen® Activity Assay
Learn More About BacMam Technology

LanthaScreen™ Tb-anti-pSTAT1 [pTyr701] Antibody Kit

A part of the JAK/STAT Pathway, STAT1 (Signal Transducer and Activator of Transcription 1) is phosphorylated at Tyr701 by the Janus family of tyrosine kinases.

For use in combination with the LanthaScreen® STAT1 U206 cell line (K1469) and the GFP-STAT1 physiological protein substrate (PV5211) to evaluate inhibitors in medium to high throughput screening applications.

LanthaScreen™ Elite Tb-anti-HIS Antibody

LanthaScreen Elite Terbium-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Tb chelates negatively impacts protein activity. The Elite Tb-anti-HIS-tag antibody is a specialized antibody with a broader HIS-tag binding performance than our traditional Tb-anti-HIS-tag antibody.

LanthaScreen™ Eu-anti-GST (Rabbit) Antibody

LanthaScreen® europium-labeled anti-GST rabbit antibodies are used to indirectly label proteins tagged with GST. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Eu chelates negatively impacts protein activity. These antibodies can be used with our Kinase Tracers to conduct LanthaScreen® Eu Kinase Binding Assays.

LanthaScreen™ Tb-pMAP2K1 (pSer217/221) Antibody

A member of the MAP Kinase family, MAP2K1 (mitogen-activated protein kinase kinase 1) is phosphorylated at Ser217⁄221 by a range of MAPK family members including Raf and B-Raf.
For use in combination with the Fluorescein-MAP2K1 physiological protein substrate (PV4812) to evaluate inhibitiors in medium to high throughput screening applications.

LanthaScreen™ Tb-anti-ubiquitin-FK2 Antibody Kit

The Tb-anti-ubiquitin-FK2 antibody preferentially binds to mono- or poly-ubiquitinated proteins over free ubiquitin. This antibody can be used in combination with purified GFP-substrates, fluorescein-ubiquitin, or LanthaScreen® cell lines to develop robust high-throughput biochemical or cell based assays for ubiquitination or deubiquitination.

LanthaScreen™ Eu-anti-DYKDDDDK Antibody

LanthaScreen Europium-labeled anti-DYKDDDDK antibodies are used to indirectly label proteins tagged with DYKDDDDK (Flag). This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Eu chelates negatively impacts protein activity.

LanthaScreen™ Tb-anti-PDCD4 [pSer457] Antibody Kit

A part of the PI3K/AKT Pathway, PDCD4 (programmed cell death protein 4) is phosphorylated at Ser457 indirectly via mTORC1 (through the ribosomal protein p70 S6 kinase).

For use in combination with the LanthaScreen® PDCD4 HEK293E cell line (K1593) to evaluate inhibitors in medium to high throughput screening applications..

LanthaScreen™ Eu-anti-GFP Antibody

These Europium-labeled anti-GFP antibodies bind specifically to GFP-tagged proteins allowing precise interrogation of protein samples. These antibodies can be used with our Kinase Tracers to conduct LanthaScreen® Eu Kinase Binding Assays.

LanthaScreen® Eu-anti-GFP Antibody Lets You:

• Indirectly label proteins tagged with GFP
• Measure the right kinase
• Interrogate active and non-activated kinases

Eu-anti-GFP Gives You the Benefits of Indirect Labeling
LanthaScreen® Europium-labeled anti-GFP antibodies can be used for indirect labeling of proteins tagged with GFP. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein negatively impacts protein activity.

Use Eu-anti-GFP Antibody in the Highly Specific LanthaScreen® Eu Kinase Binding Assay
When LanthaScreen® Eu-anti-GFP Antibody is used in the LanthaScreen® Eu Kinase Binding Assay, any signal is the result of a specific interaction of the Eu-anti-GFP antibody with the kinase of interest. Other kinases or phosphatases present without tag or with a different tag produce no signal. Unlike activity assays, the kinase binding assay can be performed with either active or non-active kinase preparations, making it ideal for detection of Type II inhibitors.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

LanthaScreen™ Tb-anti-GST Antibody

LanthaScreen® terbium-labeled anti-GST Goat antibodies are used to indirectly label proteins tagged with GST. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Tb chelates negatively impacts protein activity.

LanthaScreen™ Eu-anti-His Tag Antibody Kit

LanthaScreen® europium-labeled anti-His-tag antibodies are used to indirectly label proteins tagged with 6XHis. This can be advantageous when working with partially purified protein, or in cases in which direct labeling of the protein using amine-reactive Eu chelates negatively impacts protein activity. These antibodies can be used with our Kinase Tracers to conduct LanthaScreen® Eu Kinase Binding Assays.

LanthaScreen™ Eu-PY20 Antibody Kit

A general reagent to monitor tyrosine kinase activity, the PY20 antibody binds to phosphorylated tyrosines within a peptide or physiological protein substrate. For use in combination with AlexaFluor 647-poly-GT (PV5693) to evaluate inhibitors in medium to high throughput screening applications.
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