Shop All Magnetic Beads for Human Cell Isolation

MagniSort™ Human NK cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human NK cell Enrichment Kit is designed for the magnetic separation of NK cells by negative selection. It has been optimized for the isolation of human NK cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including NKT cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave NK cells untouched and free in solution.

The MagniSort™ Human NK cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD3 Biotin
Anti-Human CD4 Biotin
Anti-Human CD14 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD66b Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human HLA-DR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human CD4 T Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required

Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

Dynabeads™ CD8 (Invitrogen™)

Dynabeads® CD8 are superparamagnetic beads coupled with an anti-human CD8 antibody that enable easy isolation or depletion of human CD8+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD8:

• Fast and efficient isolation of CD8+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD8+ T cells for molecular downstream assays
• Fast and efficient depletion of CD8+ T cells from any sample

About Dynabeads® CD8
Dynabeads® CD8 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD8 membrane antigen mainly expressed on human cytotoxic T cells. Because of the bead size, Dynabeads® CD8 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD8 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD8 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD8 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applicationsa
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Dynabeads™ CD2 (Invitrogen™)

Dynabeads® CD2 are magnetic beads that provide a robust and versatile tool to isolate or deplete human CD2+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests.
• Rapid isolation of CD2+ cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of CD2+ cells.

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® CD2 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of human CD2+ cells directly from any sample. Dynabeads® CD2 beads are coated with a primary monoclonal antibody specific for the CD2 membrane antigen mainly expressed on human T-cells and natural killer (NK) cells. Dynabeads® CD2 bind to CD2+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD2+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® CD2 can be used in viscous samples such as whole blood and bone marrow, thereby providing a convenient method for the efficient depletion of CD2+ cells in about 30 minutes. Dynabeads® CD2 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate T-cells for cell-based applications, or you need to check your samples with flow cytometry, use Dynabeads® FlowComp™ Human CD3 (for bead-free cells) or Dynabeads® Untouched™ Human T Cells to obtain untouched human T cells.

Select what is right for you from our range of Human T Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Dynabeads™ FlowComp™ Human CD14 Kit (Invitrogen™)

Dynabeads® FlowComp™ Human CD14 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD14+ monocytes from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD14+ monocytes from any sample – no columns required
• Pure and bead-free CD14+ monocytes for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD14 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD14+ monocytes directly from any sample via a three-step process. First, the FlowComp™ Human CD14 Antibody is mixed with the sample in a tube where it binds to the CD14+ monocytes during a short incubation. Then, the CD14+ monocytes that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from the unbound cells using a magnet. Finally, the CD14+ monocytes are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD14+ monocytes.

Select what is right for you from our range of Human Monocyte Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagniSort™ Human B Cell Enrichment Kit (2nd Generation) (Invitrogen™)

The MagniSort™ Human B cell Enrichment Kit (2nd Generation) is designed for the magnetic separation of B cells by negative selection with improved purity. It has been optimized for the isolation of human B cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.

The MagniSort™ Human B cell Enrichment Antibody Cocktail (2nd Generation) contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD235a Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD8 Memory T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Memory T cell Enrichment Kit is designed for the magnetic separation of CD8 memory T cells by negative selection. It has been optimized for the isolation of human CD8 memory T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 memory T cells untouched and free in solution.

The MagniSort™ Human CD8 Memory T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RA Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human CD117 (c-Kit) Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD117 (c-Kit) Positive Selection Kit is designed for the magnetic separation of CD117+ cells by positive selection. It has been optimized for the isolation of CD117+ cells from normal human peripheral blood cells. CD117+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD117+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD117, clone YB5.B8, and Anti-Human CD34, clone 4H11.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Human CD3 Kit (Invitrogen™)

Dynabeads® FlowComp™ Human CD3 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD3+ T-cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp™ Release Buffer.
• Rapid isolation of CD3+ T-cells from any sample – no columns required
• Pure and bead-free CD3+ T-cells for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® FlowComp™ Human CD3 contains uniform, superparamagnetic beads (2.8 µm diameter) that enable easy isolation of human CD3+ cells directly from any sample via a three-step process. First, the FlowComp™ Human CD3 Antibody is mixed with the sample in a tube where it binds to the target cells during a short incubation. Then, the CD3+ T-cells that have bound the specific antibodies are captured by the FlowComp™ Dynabeads® and separated from unbound cells using a magnet. Finally, the target cells are released from the beads by adding the FlowComp™ Release buffer. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD3+ cells. The cells can be used in any downstream application.

Select what is right for you from our range of Human T Cell Isolation Products.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagniSort™ Human pan-Monocyte Enrichment Kit (Invitrogen™)

The MagniSort™ Human Pan-Monocyte Enrichment Kit is designed for the magnetic separation of conventional CD14+ and unconventional CD16+CD14- monocytes by negative selection. It has been optimized for the isolation of human pan-monocytes from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including CD16+ NK cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leaving monocytes untouched and free in solution.

The MagniSort™ Human Pan-Monocyte Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD7 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD56 Biotin
Anti-Human CD57 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Human Fc Receptor Binding Inhibitor Purified


Reported Application
Magnetic Cell Separation

Dynabeads™ CD19 Pan B (Invitrogen™)

Dynabeads® CD19 Pan B are superparamagnetic beads covalently coupled with an anti-human CD19 antibody that enable easy isolation or depletion of human CD19+ B cells directly from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD19 Pan B:

• Fast and efficient isolation of CD19+ B cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD19+ cells for molecular downstream assays or efficient depletion of CD19+ B cells
• Fast and efficient depletion of CD19+ T cells from any sample

About Dynabeads® CD19 Pan B
Dynabeads® CD19 pan B are uniform (4.5 µm diameter) superparamagnetic beads coated with a primary monoclonal antibody specific for the CD19 membrane antigen mainly expressed on human B cells. Because of the bead size, Dynabeads® CD19 pan B can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD19 pan B will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD19+ cells for cell-based applications, or you need to check your samples with flow cytometry, you can combine with DETACHaBEAD® CD19 (purchased separately to get bead- and antibody-free cells) or use Dynabeads® Untouched™ Human B Cells to obtain untouched human B cells.

Magnetic bead-based separation offers easy handling
Dynabeads® CD19 Pan B is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.

MagniSort™ Human CD56 Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD56 Positive Selection Kit is designed for the magnetic separation of CD56+ cells by positive selection. It has been optimized for the isolation of CD56+ cells from human normal peripheral blood mononuclear cells utilizing a biotinylated Anti-human CD56 antibody and streptavidin-coated magnetic beads. CD56+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD56+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD56 (clone CMSSB).

Reported Application
Magnetic Cell Separation

Dynabeads™ CD25 (Invitrogen™)

Isolate CD25+ T-cells directly from whole blood or PBMC. Dynabeads® CD25 are ideal for depleting CD25+ T-cells and for direct positive isolation for downstream molecular analysis.

• Depletion of CD25+ T-cells
• Isolation of pure and viable CD25+ T-cells for molecular applications
• No columns required

Note: For isolation of human CD4+CD25+Foxp3+ regulatory T-cells (Treg cells), please use Dynabeads® Regulatory CD4+CD25+ T Cells (Cat. no. 113-63D).

Starting samples:
Whole blood, PBMC, buffy coat.

CELLection™ Epithelial Enrich Dynabeads™ (Invitrogen™)

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells

Isolate directly from whole blood, bone marrow or PBMC
Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads® to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads® contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads® Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.

Dynabeads™ CD14 (Invitrogen™)

Dynabeads® CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD14:

• Fast and efficient isolation of CD14+ cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
• Fast and efficient depletion of CD14+ cells from any sample

About Dynabeads® CD14
Dynabeads® CD14 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads® CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD14+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human monocyte product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

Learn more about Dynabeads® products
• Find Dynabeads® products for a whole range of applications.
• Find magnets for Dynabeads® separations.