Shop All Magnetic Beads for Human Cell Isolation

MagniSort™ Human CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 T cell Enrichment Kit is designed for the magnetic separation of CD8 T cells by negative selection. It has been optimized for the isolation of human CD8 T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Human CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ CD15 (Invitrogen™)

Dynabeads® CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads® CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads® CD15:

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

About Dynabeads® CD15
Dynabeads® CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling
Dynabeads® CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

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MagniSort™ Human CD56 Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD56 Positive Selection Kit is designed for the magnetic separation of CD56+ cells by positive selection. It has been optimized for the isolation of CD56+ cells from human normal peripheral blood mononuclear cells utilizing a biotinylated Anti-human CD56 antibody and streptavidin-coated magnetic beads. CD56+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD56+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD56 (clone CMSSB).

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Human Monocytes Kit (Invitrogen™)

The Dynabeads® Untouched™ Human Monocytes Kit is used to isolate pure and viable monocytes from PBMC by negative isolation. The kit depletes T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, while the negatively isolated human monocytes are left in the sample. The isolated monocytes cells are in perfect shape for any functional assay and application. Advantages of the Dynabeads® Untouched™ Human Monocytes Kit:

• High purity, recovery, and viability of human monocytes
• Easy to use and to scale up
• No columns required
Magnetic bead-based separation offers easy handling
An antibody mix towards the non-monocytes is added to the sample and allowed to bind to the cells. After a quick wash, the Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated human monocytes can be directly analyzed in a flow cytometer and used in any application.

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MagniSort™ Human CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of human CD4 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Human CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD8 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ CD31 Endothelial Cell (Invitrogen™)

Isolate endothelial cells directly from tissue digests. Dynabeads® CD31 are ideal for positively isolating endothelial cells for downstream molecular analysis.

• Positively isolate endothelial cells directly from tissue digests for molecular applications
• Establish cultures for endothelial cells
• No columns required

Starting sample:
Tissue digests.

Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen™)

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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Dynabeads™ CD4 Positive Isolation Kit (Invitrogen™)

The Dynabeads® CD4 Positive Isolation Kit supplies superparamagnetic beads that enable gentle isolation of high-purity CD4+ T cells from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells are released from the beads using the DETACHaBEAD® reagent to obtain bead- and antibody-free cells. Advantages of the Dynabeads® CD4 Positive Isolation Kit:

• Gentle isolation of CD4+ T cells from any sample—no columns required
• Bead- and antibody-free positive isolation of CD4+ cells for any downstream assay

About Dynabeads® CD4 Positive Isolation Kit
The Dynabeads® CD4 Positive Isolation Kit contains uniform, superparamagnetic beads (4.5 µm diameter) covalently coated with a monoclonal antibody specific for the CD4 membrane antigen predominantly expressed on the helper/ inducer subset of human T cells. The kit also includes a DETACHaBEAD® reagent that releases the Dynabeads® from the purified CD4+ cells. This gently magnetic isolation process gives highly viable bead- and antibody-free cells to be used in any downstream assay.

Magnetic bead-based separation offers easy handling
Dynabeads® are mixed with the sample in a tube where they bind to the target cells during a short incubation, after which the bead-bound cells are separated from unbound cells using a magnet. The positively isolated cells are then washed, and DETACHaBEAD® reagent is added to gently release the cells from the beads. This magnetic bead-based positive isolation method gives both bead- and antibody-free cells. The isolated CD4+ cells are phenotypically unaltered and suitable for any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity and viability of the isolated CD4+ cells. For depletion or isolation of untouched CD4+ cells, select from our range of Human CD4+ T Cell Isolation Products.

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CELLection™ Epithelial Enrich Dynabeads™ (Invitrogen™)

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells

Isolate directly from whole blood, bone marrow or PBMC
Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads® to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads® contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads® Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.

MagniSort™ Human CD117 (c-Kit) Positive Selection Kit (Invitrogen™)

The MagniSort™ Human CD117 (c-Kit) Positive Selection Kit is designed for the magnetic separation of CD117+ cells by positive selection. It has been optimized for the isolation of CD117+ cells from normal human peripheral blood cells. CD117+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD117+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Human CD117, clone YB5.B8, and Anti-Human CD34, clone 4H11.

Reported Application
Magnetic Cell Separation

Dynabeads™ CD14 (Invitrogen™)

Dynabeads® CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD14:

• Fast and efficient isolation of CD14+ cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
• Fast and efficient depletion of CD14+ cells from any sample

About Dynabeads® CD14
Dynabeads® CD14 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD14 membrane antigen mainly expressed mainly on human monocytes. Because of the bead size, Dynabeads® CD14 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD14 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD14+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human monocyte product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD14 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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Dynabeads™ CD19 Pan B (Invitrogen™)

Dynabeads® CD19 Pan B are superparamagnetic beads covalently coupled with an anti-human CD19 antibody that enable easy isolation or depletion of human CD19+ B cells directly from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD19 Pan B:

• Fast and efficient isolation of CD19+ B cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD19+ cells for molecular downstream assays or efficient depletion of CD19+ B cells
• Fast and efficient depletion of CD19+ T cells from any sample

About Dynabeads® CD19 Pan B
Dynabeads® CD19 pan B are uniform (4.5 µm diameter) superparamagnetic beads coated with a primary monoclonal antibody specific for the CD19 membrane antigen mainly expressed on human B cells. Because of the bead size, Dynabeads® CD19 pan B can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD19 pan B will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD19+ cells for cell-based applications, or you need to check your samples with flow cytometry, you can combine with DETACHaBEAD® CD19 (purchased separately to get bead- and antibody-free cells) or use Dynabeads® Untouched™ Human B Cells to obtain untouched human B cells.

Magnetic bead-based separation offers easy handling
Dynabeads® CD19 Pan B is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit (Invitrogen™)

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells

Treg cells can be expanded with Dynabeads® CD3/CD28 Treg Expander and remain
Foxp3+.

Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology

The isolation procedure includes the following steps:

• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD®.

Starting sample: PBMC

Dynabeads™ Untouched™ Human B Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

MagniSort™ Human CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Human CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of human CD8 naïve T cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Human CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD4 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD36 Biotin
Anti-Human CD45RO Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Anti-Human γδ TCR Biotin

Reported Application
Magnetic Cell Separation