Shop All Magnetic Beads for Mouse Cell Isolation

MagniSort™ Mouse Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.

The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD4 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 T cell Enrichment Kit is designed for the magnetic separation of mouse CD4 T cells by negative selection. It has been optimized for the isolation of mouse CD4 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 T cells untouched and free in solution.

The MagniSort™ Mouse CD4 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD8 T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 T cell Enrichment Kit is designed for the magnetic separation of mouse CD8 T cells by negative selection. It has been optimized for the isolation of mouse CD8 T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 T cells untouched and free in solution.

The MagniSort™ Mouse CD8 T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD4 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD4 naïve T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD4 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD4 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4+CD25+ Treg Cells Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate highly pure and functional mouse Treg cells from mouse lymph nodes or spleen samples. Your cells are not exposed to the stress of being passed through a column. The isolated CD4+CD25+Foxp3+ Treg cells are bead-free and flow-compatible. The cells can be readily expanded and retain their functional suppressive capacity. You can expect a purity level of >90% Treg cells. Over 88% of the isolated CD25+ cells express Foxp3. You will save time and money, and get increased confidence in your results.

Methodology:
Negative isolation of CD4+ T cells, followed by positive isolation of CD25+ regulatory T cells. First, an antibody mix is added to the sample and the Ab will bind the non-CD4+ T cells. Mouse Depletion Dynabeads® are then added and will bind to the Ab-cell complex during a short incubation. Bead-bound cells are separated on a magnet and discarded. The remaining untouched mouse CD4+ T-cells are then labeled with FlowComp™ CD25 Antibody and positively isolated using FlowComp™ Dynabeads®. A short incubation with FlowComp™ Release Buffer then releases the isolated mouse regulatory T-cells from the Dynabeads®. The isolation method is easy to use and to scale up.

Advantages:

High purity of CD4+CD25+Foxp3+ Treg cells.

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery, and viability
• High CD25+Foxp3+ expression.
• Bead-free cells - compatible with flow cytometry analysis
• Treg cells are readily expanded and retain their functional suppressive capacity.

Starting sample:
spleen or lymp node cells

MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD8 naïve T cells from spleens or lymphnodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD8 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD8+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive mouse CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells

MagniSort™ Mouse CD11c Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD11c Positive Selection Kit is designed for the magnetic separation of CD11c+ cells by positive selection. It has been optimized for the isolation of CD11c+ cells from mouse spleens utilizing a biotinylated Anti-Mouse CD11c antibody and streptavidin-coated magnetic beads. CD11c+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD11c+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD11c, clone N418 and Anti-Mouse CD11b, clone M1/70.

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Mouse T Cells Kit (Invitrogen™)

Dynabeads® Untouched™ Mouse T Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse T cells. The negatively isolated, untouched mouse T-cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse T-cells – no columns required
• Pure and viable mouse T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that bind mouse B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-T cells. The cells are washed to remove excess antibodies, and Mouse Depletion Dynabeads® are then added. After a short incubation, the bead-bound cells are separated within 1 – 2 minutes using a magnet. The purified target mouse T-cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched T cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

MagniSort™ Mouse CD3 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD3 Positive Selection Kit is designed for the magnetic separation of CD3+ cells by positive selection. It has been optimized for the isolation of CD3+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD3 antibody and streptavidin-coated magnetic beads. CD3+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD3+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD3e, clone 145-2C11.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse Pan B (B220) (Invitrogen™)

Dynabeads® Mouse pan B (B220) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse B cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse pan B (B220) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse B cells from spleen or lymph nodes, although any single cell suspension may be used. Dynabeads® Mouse pan B beads are coated with a primary monoclonal antibody specific for the B220 membrane antigen. B220 (also known as CD45R) is expressed on all B cells throughout development, from the early pro-B stage of B cell differentiation. Dynabeads® Mouse pan B binds to B cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated B cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse pan B (B220) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable the efficient depletion of mouse B cells in about 30 minutes. Dynabeads® Mouse pan B will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that viable cells will not be released from these beads, so if you want to isolate mouse B cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® Mouse CD43 (Untouched™ B cells).

Select what is right for you from our range of Mouse B cell Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD90.2 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD90.2 Positive Selection Kit is designed for the magnetic separation of CD90.2+ cells by positive selection. It has been optimized for the isolation of CD90.2+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.2 antibody and streptavidin-coated magnetic beads. CD90.2+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.2+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD90.2, clone 53-2.1.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse B cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse B cell Enrichment Kit is designed for the magnetic separation of mouse B cells by negative selection. It has been optimized for the isolation of mouse B cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.

The MagniSort™ Mouse B cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD3 Biotin
Anti-Mouse CD4 Biotin
Anti-Mouse CD8 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD49b Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation