Shop All Magnetic Beads for Mouse Cell Isolation

Dynabeads™ Mouse CD4 (Invitrogen™)

Dynabeads® Mouse CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD4+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD4+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD4+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD4+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD4 beads are coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on mouse T helper cells. Dynabeads® Mouse CD4 binds to CD4+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD4+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD4 can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD4+ cells in about 30 minutes. Dynabeads® Mouse CD4 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use DETACHaBEAD® Mouse CD4 in combination with this products (for bead- and antibody-free cells) or Dynabeads® FlowComp Mouse CD4 Cells (complete kit for bead-free cells).

Select what is right for you from our range of Mouse CD4 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

Dynabeads™ FlowComp™ Mouse CD4 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells

MagniSort™ Mouse T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse T cell Enrichment Kit is designed for the magnetic separation of mouse T cells by negative selection. It has been optimized for the isolation of mouse T cells from spleens or lymph nodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave T cells untouched and free in solution.

The MagniSort™ Mouse T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45R (B220) Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD45R (B220) Positive Selection Kit is designed for the magnetic separation of CD45R+ cells by positive selection. It has been optimized for the isolation of CD45R+ cells from mouse spleens, bone marrow, or lymph nodes utilizing a biotinylated Anti-Mouse CD45R antibody and streptavidin-coated magnetic beads. CD45R+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD45R+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD19, clone eBio1D3 (1D3).

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Kit is designed for the magnetic separation of CD8 naïve T cells by negative selection. It has been optimized for the isolation of mouse CD8 naïve T cells from spleens or lymphnodes utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave CD8 naïve T cells untouched and free in solution.

The MagniSort™ Mouse CD8 Naïve T cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD4 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD24 Biotin
Anti-Mouse CD44 Biotin
Anti-Mouse CD45R (B220) Biotin
Anti-Mouse CD49b (Integrin alpha 2) Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse γδ TCR Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Mouse Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of mouse hematopoietic cells with committed lineages from mouse bone marrow. It utilizes a cocktail of antibody specific for hematopoietic lineages and streptavidin-coated magnetic beads. Lineage committed cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the lineage negative cells can be separated from lineage committed cells by decanting.

The MagniSort™ Mouse Hematopoietic Lineage Depletion Antibody Cocktail contains the following antibodies:
Anti-Mouse CD2 Biotin
Anti-Mouse CD3 Biotin
Anti-Mouse CD5 Biotin
Anti-Mouse CD11b Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse CD45R Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

After depletion, the enrichment of multi-potent progenitor cells can be verified by staining with Anti-Mouse CD34 (clone RAM34), and the enrichment of CD34- hematopoietic stem cells can be verified by additional staining with Anti-Mouse CD150 (clone mShad150) and Anti-Mouse CD48 (clone HM48-1).

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse Pan T (Thy1.2) (Invitrogen™)

Dynabeads® Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse pan T (Thy 1.2) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse T-cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse pan T beads are coated with a primary monoclonal antibody specific for the Thy 1.2 membrane antigen expressed on peripheral T cells, thymocytes and intraepithelial T lymphocytes of all common mouse strains. Dynabeads® Mouse pan T binds to T-cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated T cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse pan T (Thy 1.2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse T-cells in about 30 minutes. Dynabeads® Mouse pan T will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note intact cells will not be released from these beads, so if you want to isolate mouse T-cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® FlowComp Mouse Pan T (CD90.2) (for bead-free cells).

Select what is right for you from our range of Mouse T-Cell Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation