Shop All Magnetic Beads for Mouse Cell Isolation

MagniSort™ Mouse CD90.2 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD90.2 Positive Selection Kit is designed for the magnetic separation of CD90.2+ cells by positive selection. It has been optimized for the isolation of CD90.2+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.2 antibody and streptavidin-coated magnetic beads. CD90.2+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.2+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD90.2, clone 53-2.1.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

Dynabeads™ Untouched™ Mouse T Cells Kit (Invitrogen™)

Dynabeads® Untouched™ Mouse T Cells contains both magnetic beads and an Antibody Mix to deplete all leucocytes from mouse spleen or lymph node cells, leaving only the target mouse T cells. The negatively isolated, untouched mouse T-cells are pure, viable and ready to be used in any downstream application.
• Rapid isolation of untouched mouse T-cells – no columns required
• Pure and viable mouse T-cells ready for any downstream application

Excellent Yield, Purity, and Viability of Recovered Cells
The Mouse Depletion Dynabeads® suspension contains uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of cells from any single cell suspension. This reagent is therefore especially well-suited for enriching tissue digests such as mouse spleen and lymph node cells. The Mouse Depletion Dynabeads® are coated with a secondary polyclonal antibody that binds rat IgGs. The Antibody Mix contains a cocktail of rat IgGs that bind mouse B cells, NK cells, monocytes/macrophages, dendritic cells, erythrocytes and granulocytes. The Antibody Mix is first added to the sample in a tube to bind the unwanted non-T cells. The cells are washed to remove excess antibodies, and Mouse Depletion Dynabeads® are then added. After a short incubation, the bead-bound cells are separated within 1 – 2 minutes using a magnet. The purified target mouse T-cells in the supernatant can be transferred to a new tube for use in any downstream application. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the untouched T cells. The protocol can easily be scaled up according to your sample size.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.

Dynabeads™ Mouse CD8 (Lyt 2) (Invitrogen™)

Dynabeads® Mouse CD8 (Lyt 2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD8+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD8+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD8+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD8 (Lyt 2) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD8+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD8 beads are coated with a primary monoclonal antibody specific for the Lyt 2 membrane antigen mainly expressed on cytotoxic/suppressor subpopulation of mature T-cells of all common mouse strains. Dynabeads® Mouse CD8 binds to CD8+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD8+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD8 (Lyt 2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD8+ cells in about 30 minutes. Dynabeads® Mouse CD8 (Lyt 2) will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® FlowComp Mouse CD8 Cells (for bead-free cells).

Select what is right for you from our range of Mouse CD8 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD90.1 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD90.1 Positive Selection Kit is designed for the magnetic separation of CD90.1+ cells by positive selection. It has been optimized for the isolation of CD90.1+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.1 antibody and streptavidin-coated magnetic beads. CD90.1+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.1+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse/Rat CD90.1, clone HIS51.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD8 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Positive Selection Kit is designed for the magnetic separation of CD8+ cells by positive selection. It has been optimized for the isolation of CD8+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD8a antibody and streptavidin-coated magnetic beads. CD8+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD8+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD8a, clone 53-6.7.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4+CD25+ Treg Cells Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate highly pure and functional mouse Treg cells from mouse lymph nodes or spleen samples. Your cells are not exposed to the stress of being passed through a column. The isolated CD4+CD25+Foxp3+ Treg cells are bead-free and flow-compatible. The cells can be readily expanded and retain their functional suppressive capacity. You can expect a purity level of >90% Treg cells. Over 88% of the isolated CD25+ cells express Foxp3. You will save time and money, and get increased confidence in your results.

Methodology:
Negative isolation of CD4+ T cells, followed by positive isolation of CD25+ regulatory T cells. First, an antibody mix is added to the sample and the Ab will bind the non-CD4+ T cells. Mouse Depletion Dynabeads® are then added and will bind to the Ab-cell complex during a short incubation. Bead-bound cells are separated on a magnet and discarded. The remaining untouched mouse CD4+ T-cells are then labeled with FlowComp™ CD25 Antibody and positively isolated using FlowComp™ Dynabeads®. A short incubation with FlowComp™ Release Buffer then releases the isolated mouse regulatory T-cells from the Dynabeads®. The isolation method is easy to use and to scale up.

Advantages:

High purity of CD4+CD25+Foxp3+ Treg cells.

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery, and viability
• High CD25+Foxp3+ expression.
• Bead-free cells - compatible with flow cytometry analysis
• Treg cells are readily expanded and retain their functional suppressive capacity.

Starting sample:
spleen or lymp node cells

Dynabeads™ Mouse CD4 (Invitrogen™)

Dynabeads® Mouse CD4 are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD4+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD4+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD4+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD4 are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD4+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD4 beads are coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on mouse T helper cells. Dynabeads® Mouse CD4 binds to CD4+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD4+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD4 can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD4+ cells in about 30 minutes. Dynabeads® Mouse CD4 will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use DETACHaBEAD® Mouse CD4 in combination with this products (for bead- and antibody-free cells) or Dynabeads® FlowComp Mouse CD4 Cells (complete kit for bead-free cells).

Select what is right for you from our range of Mouse CD4 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.