Shop All Magnetic Beads for Mouse Cell Isolation

MagniSort™ Mouse CD90.1 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD90.1 Positive Selection Kit is designed for the magnetic separation of CD90.1+ cells by positive selection. It has been optimized for the isolation of CD90.1+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.1 antibody and streptavidin-coated magnetic beads. CD90.1+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.1+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse/Rat CD90.1, clone HIS51.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD8 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD8+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD8+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive mouse CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells

MagniSort™ Mouse CD49b Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD8 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD8 Positive Selection Kit is designed for the magnetic separation of CD8+ cells by positive selection. It has been optimized for the isolation of CD8+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD8a antibody and streptavidin-coated magnetic beads. CD8+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD8+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD8a, clone 53-6.7.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Mouse CD45 Depletion Kit is designed for the magnetic depletion of mouse CD45+ cells from mouse spleens, bone marrow or digested lung tissue. It utilizes a biotinylated Anti-Mouse CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of enriched CD45- cells can be verified by staining with Anti-Mouse CD45, clone 30-F11.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD11c Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD11c Positive Selection Kit is designed for the magnetic separation of CD11c+ cells by positive selection. It has been optimized for the isolation of CD11c+ cells from mouse spleens utilizing a biotinylated Anti-Mouse CD11c antibody and streptavidin-coated magnetic beads. CD11c+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD11c+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD11c, clone N418 and Anti-Mouse CD11b, clone M1/70.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD4 Positive Selection Kit (Invitrogen™)

The MagniSort™ Mouse CD4 Positive Selection Kit is designed for the magnetic separation of CD4+ cells by positive selection. It has been optimized for the isolation of CD4+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD4 antibody and streptavidin-coated magnetic beads. CD4+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD4+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD4, clones RM4-5 or GK1.5.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells