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TaqMan™ GTXpress™ Master Mix (Applied Biosystems™)

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing genotyping master mix for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan GTXpress Master Mix and added additional capabilities for your genotyping experiments.

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TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 10 mL pack includes reagents sufficient to perform 4,000 PCR reactions (5 µL/reaction).
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling can be used for poorly amplified or sample-limited reactions The TaqMan® GTXpress™ Master Mix is also sold as part of TaqMan® Sample-to-SNP™ Kit, which provides real-time PCR-ready DNA in 5 min from a wide variety of samples, ranging from blood to buccal swabs to plant tissues. For more information on the TaqMan® Sample-to-SNP™ Kit, refer to the Applied Biosystems® TaqMan® Sample-to-SNP™ Kit Protocol (PN. 4402136).

TaqMan™ OpenArray™ Genotyping Master Mix (Applied Biosystems™)

This 2X concentrated mix is optimized for SNP genotyping using TaqMan® OpenArray® SNP Genotyping Plates.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Genotyping Master Mix (Applied Biosystems™)

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation experiments.

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TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis
The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000

4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 µL Reactions: 800
• 20 µL Reactions: 2,000
• 10 µL Reactions: 4,000
• 5 µL Reactions: 8,000

4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL)
• 50 µL Reactions: 2000
• 20 µL Reactions: 5,000
• 10 µL Reactions: 10,000
• 5 µL Reactions: 20,000

4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL)
• 50 µL Reactions: 4000
• 20 µL Reactions: 10,000
• 10 µL Reactions: 20,000
• 5 µL Reactions: 40,000

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

SNaPshot™ Multiplex Kit, with protocol (Applied Biosystems™)

The SNaPshot® Multiplex System is a primer extension-based method that enables multiplexing up to 10 SNPs (single nucleotide polymorphisms). Use this system to screen and confirm SNPs, assess DNA methylation, fingerprint BACs, and screen samples for susceptibility to scrapie.

• Perform multiplexed, single-base extension of up to 10 SNPs.
• Interrogate multiple SNPs regardless of chromosome position.
• Separate SNP loci that differ by a single base pair.
• Perform robust interrogation of multiple SNPs with only a small amount of amplified template.
• Decrease expenses with this moderately priced system.
• Reduce the complexity of your experiments with this well-tested, easy-to-understand single-base extension technique.

Screen and Confirm SNPs
The SNaPshot® Multiplex System is the perfect tool for SNP screening and validation. The kit offers a one-tube single-base extension⁄termination reagent to label DNA fragments. First, run DNA fragments on any Applied Biosystems capillary electrophoresis instrument with the GeneScan™-120 LIZ® Size Standard to determine the size of labeled fragments. Then use GeneMapper® Software to analyze the data and generate allele calls. The SNaPshot® Multiplex Kit allows multiplexing during single-base extension of up to 10 primer-template combinations in a single tube-single capillary format.

Assess DNA Methylation
The study of methylation⁄epigenetics is emerging as an important component of cancer research. In a typical assay to detect methylation, bisulfite treatment of DNA deaminates non-methylated cytosine and converts it to uracil while methylated cytosine remains unreactive. The subsequent step of PCR amplification coverts uracil bases to thymine. Use SNaPshot® to quantitatively detect the base differences in treated and untreated samples to learn the methylation status of your samples.

Fingerprint BACs
New BAC libraries require a rapid, efficient method for characterization (also called fingerprinting) and assembly of clones into contigs (contiguous consensus sequences), which are then arrayed into physical maps of the chromosome. Use the SNaPshot® Multiplex Kit to fingerprint clones by labeling BAC fragments after digestion with restriction endonucleases. The labeled fragments can then be separated and detected on any Applied Biosystems capillary electrophoresis instrument. Sizing information from GeneMapper® Software v3.5 or higher is imported into subsequent editing and contig assembly programs. The high-quality results you get from the SNaPshot® Multiplex Kit provide an easy-to-use and cost-effective solution for high-throughput BAC fingerprinting.

Screen Samples for Susceptibility to Scrapie
Scrapie, like other prion diseases are caused by abnormally-folded isoforms of host-encoded proteins. The single base-pair sensitivity of the SNaPshot® system enables you to screen samples accurately for codon differences in prion genes. Use the SNaPshot® Multiplex System to screen for SNPs in the genes that code for these proteins. For instance, polymorphisms at codons 136, 154 and 171 of the PrP gene in sheep and goats can lead to abnormally folded isoforms of the protein product to result in scrapie.

For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 12K Flex OpenArray™ Practice Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Practice Kit is designed to introduce newcomers to the OpenArray® block workflow, as well as to provide instrument verification. The kit contains 6 practice loading arrays plus reagents. The practice kit also includes these accessories: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™tips, and 384-well plates.

TaqMan™ Sample-to-SNP™ Kit (200 mL sample prep, 10 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hr. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 4,000 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

OncoScan™ CNV Plus Reagent Kit (Applied Biosystems™)

The OncoScan CNV Plus Reagent Kit is part of the OncoScan CNV Plus Assay and so is used in conjunction with OncoScan CNV Plus arrays. The OncoScan CNV Plus Reagent Kit contains somatic mutation probe mix, copy number probe mix and controls, gap fill and 1st stage PCR reagents, 2nd stage PCR and post PCR processing reagents, stain reagents, and wash buffers.

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 µL/ reaction and 400 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 20,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

QuantStudio™ 12K Flex OpenArray™ Genotyping Starter Kit (Applied Biosystems™)

A variety of starter kits are available to introduce you to the various applications of the QuantStudio™12K Flex OpenArray® sample block. Each starter kit contains 2 arrays, plus sufficient reagents and accessories to perform two experiments. The Genotyping starter kit contains 2 genotyping training arrays. Note that the QuantStudio™12K Flex Software (included with the QuantStudio™ 12K Flex System) offers example experiment files for each application.

The accessories provided in the kit include: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

OncoScan™ CNV Reagent Kit (Applied Biosystems™)

The OncoScan CNV Reagent Kit is part of the OncoScan CNV Assay and so is used in conjunction with OncoScan CNV arrays. The OncoScan CNV Reagent Kit contains the copy number probe mix and controls, gap fill and 1st stage PCR reagents, 2nd stage PCR and post PCR processing reagents, stain reagents, and wash buffers.