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MAXIscript™ SP6 Kit with Manual (Invitrogen™)

The Ambion® MAXIscript® SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.

MAXIscript™ SP6 Transcription Kit (Invitrogen™)

The Ambion® MAXIscript® SP6 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 100 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.

TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific™)

Thermo Scientific TranscriptAid T7 High Yield Transcription Kit contains reagents for 50 reactions of 20 µL. Each reaction yields up to 200 µg RNA from 1 µg of template in 2 hours. The reaction can be scaled up to produce milligram amounts of full-length RNA. The kit can be used to produce both long and short transcripts for applications that require large yields of RNA. All necessary reagents for transcription are included, as well as the RiboRuler High Range RNA Ladder for sizing and quantification.

Highlights

Exceptionally high yields—up to 200 µg in 2 hours
Versatile—suitable for both short and long RNA transcripts
Milligram amounts of RNA in a single, scaled-up reaction
Flexible—generates unlabeled, labeled or capped RNA
RiboRuler RNA Ladder supplied with kit for sizing and quantification

Applications

In vitro transcription
In vitro translation
• Generation of hybridization probes for:
• microarrays
in situ hybridization
• blotting

• RNase protection assays
• RNA binding protein assays
• Antisense RNA and RNAi
• RNA amplification
• Microinjection studies

Includes

• TranscriptAid Enzyme Mix
• 5X TranscriptAid Reaction Buffer
• DNase I, RNase-free
ATP Solution
CTP Solution
GTP Solution
UTP Solution
• Control template
• 3 M Sodium Acetate Solution, pH 5.2
• DEPC-treated Water
• 2X RNA Loading Dye
RiboRuler High Range RNA Ladder, ready-to-use
• 0.5 M EDTA, pH 8.0
• Detailed Protocol

* The improved version of TranscriptAid T7 High Yield Transcription Kit contains Tris-buffered NTPs instead of previously used NTPs titrated with KOH. Usage of Tris-buffered NTPs leads to higher yields of RNA transcripts.

MEGAscript™ T7 Transcription Kit (Invitrogen™)

The MEGAscript® T7 Kit is an ultra-high yield in vitro transcription kit. The high yields are achieved by modifying typical transcription reaction conditions so that very high nucleotide concentrations can be effectively used. The SP6, T7, or T3 Enzyme Mix and the 10X Reaction Buffer are specifically calibrated for each lot and RNA polymerase.

Using MEGAscript® Kits
The MEGAscript® Kits contain in vitro transcription reaction components for twenty-five or forty 20 µL reactions and a control template. Each kit will yield a total of 3–5 mg of RNA (approximately 100 µg of RNA or more per reaction) from the control template supplied with the kit. This corresponds to 400–650 moles of RNA for each mole of template. Smaller templates typically yield a lower mass and a higher molar yield of product. Novel transcription reaction conditions and a patented, high-yield technology, allow the synthesis of 10–50 times the amount of RNA produced by conventional transcription reactions.

Applications
MEGAscript® Kits are intended for the synthesis of large amounts of unlabeled or low specific activity RNA for a variety of uses, including in vitro translation, antisense/microinjection studies, and isolation of RNA binding proteins. In large-scale transcription reactions, the concentration of all 4 nucleotides is high, well above the Km for the enzyme. MEGAscript® Kits typically yield over 10 times more RNA than conventional in vitro transcription reactions. MEGAscript® Kits are not recommended for synthesis of high specific activity probes.

Accessory products
MessageAmp™ aRNA Amplification Kits are powered by MEGAscript® technology and have been developed especially for the amplification of cRNA prior to array analysis. To purify in vitro transcription products from free nucleotides, buffer components, and enzymes, we recommend using the MEGAclear™ Kit (Cat. No. AM1908) and the MEGAclear™-96 Kit (Cat. No. AM1909).

MAXIscript™ T7 Transcription Kit (Invitrogen™)

The Ambion® MAXIscript® T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.

MEGAshortscript™ T7 Transcription Kit (Invitrogen™)

The MEGAshortscript™ T7 Kit is designed for high yields of in vitro- transcribed RNA products, in the 20–500 nucleotide range. Using conventional methods, transcription of small templates often results in low mass yields of RNA. Short transcripts require many more transcription initiation events to synthesize a given mass of product. Since the initiation step is the rate-limiting step in transcription reactions, the yield of product from short templates is often low. The MEGAshortscript™ T7 Kit has been designed to overcome the drawbacks involved in conventional in vitro transcription to maximize the yield of short RNA transcripts.

Advantages of the MEGAshortscript™ Kit:

• Efficiently transcribes small templates (<300 bases) with consistently high yields
• Designed for use with plasmid, synthetic oligonucleotide, or PCR product templates

Note:The MEGAshortscript™ T7 Kit is optimized for use with the T7 polymerase included in the kit. Using a different polymerase may result in low yields.

mMESSAGE mMACHINE™ T3 Transcription Kit (Invitrogen™)

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions.
• High yields of capped RNA—up to 39 µg of RNA per reaction
• Extra convenience—cap analog-nucleotide mix provided
• Simplified reaction format
• Synthesized transcripts ideal for microinjection, in vitro translation, or transfection In a 20 µl reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield up to 35 µg of capped RNA. This is 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). The mMESSAGE mMACHINE® kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® Kits are only optimized for use with the polymerases included in the kit. Using a different polymerase than what is supplied in the kit may result in low yields. Each mMESSAGE mMACHINE® Kit contains 57 U of cap analog, an outstanding value for the cost.

mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (Invitrogen™)

The mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog —Anti-Reverse Cap Analog (ARCA)—with a patented high-yield transcription technology, to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. The kit includes sufficient reagents for 50 reactions. Advantages of the mMESSAGE mMACHINE® T7 Ultra Kit:

• Synthesize more protein from in vitro-transcribed RNA
• Express RNA transcripts more efficiently both in vitro and in vivo
• Stabilize transcripts in vivo with included reagents for poly(A) tailing

ARCA-capped RNA is translationally more active
A base modification in ARCA results in its incorporation in the functional, translatable orientation only; traditional cap analog can be incorporated in both functional and nonfunctional orientations. As a result, incorporating ARCA into transcription reactions yields capped RNAs that are 100% translatable. This is further enhanced by the inclusion of poly(A) tails in mMESSAGE mMACHINE® T7 Ultra transcripts. Experiments comparing ARCA and ARCA/poly(A)–tailed transcripts to cap analog and cap analog/poly(A)–tailed transcripts indicate higher levels of protein synthesis with ARCA capped RNA .

What Is ARCA?
Anti-Reverse Cap Analog (ARCA) is a modified cap analog in which the 3' OH group (closer to m7G) is replaced with OCH3 (see schematic). Because of this substitution, the RNA polymerase can only initiate transcription with the remaining hydroxyl group, thus forcing ARCA incorporation in the forward orientation. As a result, 100% of the transcripts synthesized with ARCA at the 5' end are translatable, leading to a strong stimulatory effect on translation.

Proper capping of in vitro transcribed RNA
Proper capping of RNA promotes correct initiation of protein synthesis, as well as stability and processing of mRNA in vivo. Uncapped RNA is rapidly degraded by cellular RNases after microinjection or transfection into cells. Capped RNA is also typically translated more efficiently in in vitro translation systems, generating RNAs with cap analog incorporated only in the functional orientation. Therefore, substitution of traditional cap analog with ARCA results in the synthesis of capped RNAs that are 100% translatable.

MAXIscript™ SP6/T7 Transcription Kit (Invitrogen™)

The Ambion® MAXIscript® SP6/T7 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 50 reactions of each enzyme.

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.

Precision gRNA Synthesis Kit (Invitrogen™)

The Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with TrueCut™ Cas9 Protein v2 for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP). This Cas9 RNP format, with our TrueCut Cas9 Protein v2, has been tested in a variety of suspension and adherent cell lines with >70% cleavage efficiencies and no indications of toxicity. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short ‘one-pot’ PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect Invitrogen™ CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the Precision gRNA Synthesis Kit include:
• Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
• High yield (>10 ug) and concentration (>200 ng/uL) of gRNA

How to obtain a gRNA sequence
Genome editing with CRISPR technology requires a noncoding guide RNA (gRNA) in order to cleave genomic DNA at a target sequence of interest. The gRNA has two molecular components: a target-specific CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) that have been combined into one transcript. The target sequence (20 bases) must be immediately upstream of a PAM motif (NGG) which allows the Cas9 to initiate binding. The PAM is only on the target DNA and not part of the target specific CRISPR sequence. The gRNA and the PAM motif guide the Cas9 nuclease to the target genomic sequence to form a complex and create a double-stranded blunt DNA break (DSB) three nucleotides upstream from the PAM site.

Use our CRISPR Search and Design Tool to search our database of >600,000 gRNA sequences specific to every gene in the human and mouse genomes. Invitrogen predesigned gRNAs are optimized for gene knockout and typically target the first three transcribed exons per gene. Search results include recommendations based on minimizing potential off-target effects, potential binding sites, and exon maps with gRNA locations. This tool can also be used to analyze any sequence of interest to design unique CRISPR sequences.

How to make gRNA
Once gRNA sequences have been selected, choose from three options for making gRNA:
1. TrueGuide synthetic guide RNA—choose from our catalog of predesigned gRNAs or upload your sequence to our TrueGuide gRNA Ordering Tool
2. Precision gRNA Synthesis Kit (this page)—for transfection-ready gRNA in as little as four hours including template assembly
3. Genome Engineering Services—save time and effort and have our custom services team design, synthesize, and purify in vitro transcribed (IVT) gRNA sequences for you. To obtain a services quotation, or to order, please contact our Custom Services department at 1-800-955-6288 x45682 or gemservices@thermofisher.com.

Poly(A) Tailing Kit (Invitrogen™)

For the polyadenylation of in vitro transcribed RNA to enhance translation initiation efficiency. Sufficient reagents are included for 25 reactions.

• Adds a poly(A) tail of at least 150 nucleotides in length to the 3' termini of RNA
• Enhances translational efficiency in vivo
• Reaction can be adjusted to control for tail length
• Optimized for use with RNA transcripts synthesized using the mMESSAGE mMACHINE™ Kit

The Poly(A) Tailing Kit uses E. coli Poly(A) Polymerase I (E-PAP) to polyadenylate the 3'-termini of in vitro transcribed RNA. Polyadenylation plays an important role in the stabilization of RNA in eukaryotes and enhances the efficiency of translation initiation. In the Poly(A) Tailing Kit, the E-PAP reaction has been optimized so that mRNAs are efficiently tailed with at least 150 adenines. The additional adenine residues confer stability to the mRNA and may increase translational efficiency of in vitro synthesized capped RNA in microinjection and transfection experiments (see Figure).

Accessory Products:
The Poly(A) Tailing Kit is optimized for use with Ambion's mMESSAGE mMACHINE™ High Yield Capped RNA Transcription Kit. mMESSAGE mMACHINE™ Kits are available with T7 (SKU #AM1334), T3 (SKU #AM1338), or SP6 (SKU #AM1330) polymerases.

mMESSAGE mMACHINE™ T7 ULTRA Transcription Kit (Invitrogen™)

The mMESSAGE mMACHINE® T7 Ultra Kit combines a new cap analog, anti-reverse cap analog (ARCA), with a patented high-yield transcription technology to generate RNA transcripts that produce higher protein yields compared to other transcripts upon translation. Advantages of the mMESSAGE mMACHINE® T7 Ultra Kit:

• Synthesize more protein from in vitro-transcribed RNA
• Express RNA transcripts more efficiently both in vitro and in vivo
• Stabilize transcripts in vivo with included reagents for poly(A) tailing

ARCA-capped RNA is translationally more active
A base modification in ARCA results in its incorporation in the functional, translatable orientation only; traditional cap analog can be incorporated in both functional and nonfunctional orientations. As a result, incorporating ARCA into transcription reactions yields capped RNAs that are 100% translatable. This is further enhanced by the inclusion of poly(A) tails in mMESSAGE mMACHINE® T7 Ultra transcripts. Experiments comparing ARCA and ARCA/poly(A)-tailed transcripts to cap analog and cap analog/poly(A)-tailed transcripts indicate higher levels of protein synthesis with ARCA capped RNA .

What Is ARCA?
ARCA is a modified cap analog in which the 3' OH group (closer to m7G) is replaced with OCH3 (see schematic). Because of this substitution, the RNA polymerase can only initiate transcription with the remaining hydroxyl group, thus forcing ARCA incorporation in the forward orientation. As a result, 100% of the transcripts synthesized with ARCA at the 5' end are translatable, leading to a strong stimulatory effect on translation.

Proper capping of in vitro transcribed RNA
Proper capping of RNA promotes correct initiation of protein synthesis, as well as stability and processing of mRNA in vivo. Uncapped RNA is rapidly degraded by cellular RNases after microinjection or transfection into cells. Capped RNA is also typically translated more efficiently in in vitro translation systems, generating RNAs with cap analog incorporated only in the functional orientation. Therefore, substitution of traditional cap analog with ARCA results in the synthesis of capped RNAs that are 100% translatable.

Poly(A) Tail-Length Assay Kit (Thermo Scientific™)

Newly reconfigured kit for increased efficiency: 76455 1 KT Poly(A) Tail-Length Assay Kit is a direct replacement for 76450 1 KT

• Measure the poly(A) tail of multiple RNAs in under 3 hrs using common lab equipment
• Accurate measurement of poly(A) tail length compared to other assays (e.g. northern blotting)
• One kit includes optimized reagents for 5 G/I Tailing, 20 RT and 80 PCR reactions

Description:
Poly adenylation of eukaryotic mRNAs plays a critical role in RNA metabolism including stability, enhanced translation, nuclear export and miRNA mediated gene regulation. The Poly(A) Tail-Length Assay Kit allows you to quickly measure the poly(A) tails of multiple mRNAs in only three hours. This kit utilizes a novel G/I extension technique which preserves the 3' end of RNAs and ensures accurate measurement of poly(A) tail-length. And, all reaction components have been optimized so you get quality data quickly.

Description:
The Poly(A) Tail-Length Assay Kit uses four key steps to enable poly(A) tail-length determination (Fig. 1).

In Step 1, poly(A) polymerase adds a limited number of guanosine and inosine residues to the 3’-ends of poly(A)-containing RNAs.

In Step 2, the tailed-RNAs are converted to DNA through reverse transcription using the newly added G/I tails as the priming sites.

In Step 3, PCR amplification products are generated using two primer sets. A gene-specific forward and reverse primer set designed upstream of the polyadenylation site (e.g. the 3’-UTR) is produced as a control for the gene-of-interest. The second set of primers uses the gene-specific forward primer and the universal reverse primer provided with the kit to generate a product that includes the poly(A) tails of the gene-of-interest.

Finally, in Step 4, the PCR products are separated on an agarose or polyacrylamide gel (Fig. 2).

Kit components:
Kit contains all necessary reagents for 5 G/I Tailing, 20 reverse transcription, and 80 PCR reactions.

Selected References:
Parker, R. and Song, H. (2004) Nat Struct Mol Biol. 11 121-127.
Brodersen, D.E. et al.  (2008) Biochim Biophys Acta. 1779, 532-537.
Wu, L., Fan J. and Belasco, J.G. (2006) Proc Natl Acad Sci U S A. 103, 4034-4039.
Izaurralde, E. et al. (2009) RNA 15, 21-32.
Martin, G. and Keller, W. (1998) RNA 4, 226-230.
Gauss-Müller, V. et al. (2001) Nucleic Acids Res. 29 E57-7

mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen™)

mMESSAGE mMACHINE® kits are designed for the in vitro synthesis of large amounts of capped RNA. Capped RNA mimics most eukaryotic mRNAs found in vivo, because it has a 7-methyl guanosine cap structure at the 5' end. mMESSAGE mMACHINE® kit reactions include cap analog [m7G(5')ppp(5')G] in an ultra high-yield transcription reaction. The cap analog is incorporated only as the first or 5' terminal G of the transcript because its structure precludes its incorporation at any other position in the RNA molecule.

How mMESSAGE mMACHINE® kits work
mMESSAGE mMACHINE® kits have a simplified reaction format in which all four ribonucleotides and cap analog are mixed in a single solution. The cap analog:GTP ratio of this solution is 4:1, which is optimal for maximizing both RNA yield and the proportion of capped transcripts. mMESSAGE mMACHINE® kits are ideal for the routine synthesis of capped RNAs for oocyte microinjection, in vitro translation, transfection, and other applications. The high yields are achieved by optimizing reaction conditions for RNA synthesis in the presence of high nucleotide concentrations. In addition, the RNA synthesized is protected from degradation by any contaminating ribonucleases that may be present with RNase inhibitor—a component of the Enzyme Mix.

Using mMESSAGE mMACHINE® Kits
In a 20 µL reaction during a 2 hour incubation, mMESSAGE mMACHINE® High Yield Capped RNA Transcription will yield large mass amounts of capped RNA. Up to 10–50 times the yield obtained with conventional in vitro transcription reactions. The ratio of cap analog to GTP has been optimized to allow the best compromise between yield (15–35 µg) and proportion of transcripts that are capped (80%). mMESSAGE mMACHINE® Kits also contain a LiCl precipitation solution that is efficient for separating proteins and unincorporated nucleotides (including free cap analog) from the capped RNA, allowing an increased efficiency of translation. mMESSAGE mMACHINE® kits are only optimized for use with the polymerases included in the kit. Using a different polymerase may result in low yields. The mMESSAGE mMACHINE® kits contain all the buffers and reagents necessary for 25 transcription reactions. Using the control template supplied with the kits (Xenopus elongation factor 1α, pTRI Xef), each mMESSAGE mMACHINE® reaction will yield approximately 20–30 µg of RNA using T3 or T7 RNA polymerase, or about 15–25 µg RNA using SP6 RNA polymerase.

MAXIscript™ T3 Transcription Kit (Invitrogen™)

The Ambion® MAXIscript® T3 In Vitro Transcription Kit synthesizes RNA probes in just 10 minutes for use in ribonuclease protection assays, in situ hybridization, and blot hybridizations. Each kit contains sufficient reagents for 30 reactions.

Features:

• High yields of high specific activity, full-length RNA probes in just 10 minutes
• Ideal probe synthesis kit for nuclease protection assays, northern and Southern blots, and in situ hybridization
• Includes TURBO DNase™
• All kits contain quality-tested DNase/RNase-free reagents

The MAXIscript® In Vitro Transcription Kit synthesizes RNA probes with specific activities reaching 1 × 109 cpm/µg in just 10 minutes. MAXIscript® Kits are very efficient at synthesizing full-length probes, even at limiting nucleotide concentrations, making them the perfect companion to ribonuclease protection assays. MAXIscript® Kits are also an excellent choice for the synthesis of nonisotopically labeled probes. Labeling can be accomplished post-transcriptionally by using Ambion's BrightStar® Psoralen-Biotin Labeling Kit, or during transcription by incorporating modified nucleotides into the reaction. MAXIscript® Kits can also be used for the synthesis of modest amounts of unlabeled RNA (2–6 µg/20 µl reaction) of up to 2 kb.