Shop All Real-Time PCR Instrument Calibration Kits & Reagents

ViiA™ 7 Array Card Spectral Calibration Kit (Applied Biosystems™)

This kit is used to perform spectral calibration, region-of-interest (ROI) calibration, and normalization on your ViiA™ 7, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with array card block. It includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC® dye, ROX™ dye, ROI dye mix, background buffer, FAM™⁄ROX™ dye, VIC®⁄ROX™ dye. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TaqMan™ RNase P Instrument Verification Plate for 7900HT System, 384-well (Applied Biosystems™)

The Applied Biosystems® TaqMan® RNase P 384-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR System. The TaqMan® RNase P 384-Well Instrument Verification Plate is a 384-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template.
The TaqMan® RNase P Instrument 384-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7900 HT Real-Time PCR Systems installation. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

SYBR™ Green Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This SYBR® Green dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

7500 Fast Real-Time PCR Systems Spectral Calibration Kit I (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for FAM™⁄SYBR® Green I, VIC®⁄JOE™, and NED™⁄TAMRA™⁄ROX™ dyes on the 7500 Fast Real-Time PCR System.

The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains nine Optical 96-Well Fast Thermocycling Plates: one preloaded and sealed background plate, seven preloaded and sealed plates with seven separate dye standards, and one region-of-interest (ROI) plate.

• 96-well optical reaction plates preloaded with dyes (FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄TAMRA™⁄ROX™) allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously
• Includes the Passive Reference I dye standard required for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research — Not Instrument Calibration

This kit's easy-to-use, preloaded 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

Normalization Kit, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Normalization Kit is used to maintain your real-time PCR instrument with Fast 96-well block. Normalization plates are used to calibrate different instruments. The kit contains one FAM™/ROX™ normalization plate and one VIC®/ROX™ normalization plate. Please see your instrument user guide for full instructions on use.

Region of Interest (ROI) and Background Plates, 384-well (Applied Biosystems™)

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 384-well blocks. See the instrument maintenance guide for full instructions on use.

TaqMan™ RNase P Instrument Verification Plate, 96-well (Applied Biosystems™)

The TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7900HT 96-Well Real-Time PCR System and the ABI PRISM® 7000 and 7700 Sequence Detection Systems. The TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The TaqMan® RNase P Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7900HT 96-Well Real-Time PCR System, and the ABI PRISM® 7000 and 7700 Sequence Detection Systems installations. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

ABI PRISM™ 7000 Sequence Detection Systems Spectral Calibration Kit (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™ dyes on the ABI PRISM® 7000 Sequence Detection System. The ABI PRISM® 7000 Sequence Detection Systems Spectral Calibration Kit includes eight optical 96-well reaction plates: one preloaded and sealed background plate, and seven preloaded and sealed spectral calibration plates with seven separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™) are provided for quick and easy calibration of the ABI PRISM® 7000 Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the ABI PRISM® 7000 Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

TAMRA™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This TAMRA™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your 7500, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 3 (TAMRA™, NED™, Cy™5 dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for TAMRA™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

MUSTANG PURPLE™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® spectral calibration of your Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, or QuantStudio™ 12K Flex real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.

FAM™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.