Shop All Real-Time PCR Instrument Calibration Kits & Reagents

FAM™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ABY™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for ABY® spectral calibration of your Applied Biosystems® 7500 or ViiA™ 7 real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.

VIC™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This VIC® dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

JUN™ Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for JUN® spectral calibration of your Applied Biosystems® 7500 or ViiA™ 7 real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.

ROX™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This ROX™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Sequence Detection Systems Spectral Calibration Kit, 96-well (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR® Green, TAMRA™, TET™, and VIC™ dyes on the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 96-Well Calibration kit includes three optical 96-well reaction plates: one preloaded and sealed background plate, and two preloaded and sealed spectral calibration plates with eight separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™,ROX™, SYBR® Green , TAMRA™, TET™, and VIC™) are provided for quick and easy calibration of the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

MUSTANG PURPLE™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® spectral calibration of your Applied Biosystems® 7500, ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, or QuantStudio™ 12K Flex real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.

SYBR™ Green Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This SYBR® Green dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your 7500, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

SDS Spectral Calibration Kit (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™ dyes on the ABI PRISM 7700® Sequence Detection System.

The SDS Spectral Calibration kit includes 8 tubes of pure dye standards for the Applied Biosystems® ABI PRISM 7700® Sequence Detection System.

• Individual tubes containing eight dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™) are provided for quick and easy calibration of the ABI PRISM 7700® Sequence Detection System
• Individual components provide flexibility in dye standards setup



• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration


This kit enables users to calibrate the ABI PRISM 7700® Sequence Detection System quickly and easily. The system automatically collects the dye standard's spectral information as part of the instrument installation procedure. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well) (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ Real-Time PCR System.

The Applied Biosystems® StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the StepOnePlus™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the StepOnePlus™ Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

QuantStudio™ 5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy™5 dyes), 384-well (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 5 384-well real-time PCR system. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

MeltDoctor™ HRM Calibration Plate, 384-well (Applied Biosystems™)

Applied Biosystem® ready-to-use MeltDoctor™ HRM Calibration Plates contain all the components required for pure dye and HRM calibration, reducing the complexity of getting started with HRM experimentation. These plates provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves.

TAMRA™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This TAMRA™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

ABI PRISM™ 7000 Sequence Detection Systems Spectral Calibration Kit (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™ dyes on the ABI PRISM® 7000 Sequence Detection System. The ABI PRISM® 7000 Sequence Detection Systems Spectral Calibration Kit includes eight optical 96-well reaction plates: one preloaded and sealed background plate, and seven preloaded and sealed spectral calibration plates with seven separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR® Green, VIC™, and TAMRA™) are provided for quick and easy calibration of the ABI PRISM® 7000 Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the ABI PRISM® 7000 Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

Array Card Calibration Install Kit (Applied Biosystems™)

This kit is used to maintain your ViiA™ 7 System with TaqMan® Array Card Block. It includes reagents to perform a spectral calibration with FAM™, VIC®, and ROX™ dyes, region-of-interest (ROI) and normalization calibration, and to run an RNase P verification card. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7 System will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

VIC™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for VIC® spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TaqMan™ RNase P Instrument Verification Plate, Fast 48-well (Applied Biosystems™)

Applied Biosystems® TaqMan® RNase P Fast 48-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® StepOne™ Real-Time PCR System. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate is a 48-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® StepOne™ Real-Time PCR Systems installation. To pass installation, the instrument must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

TAMRA™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for TAMRA™ spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

MeltDoctor™ HRM Calibration Standard (20X) (Applied Biosystems™)

Applied Biosystems® MeltDoctor™ HRM Calibration Standard contains a DNA template and primers which, when mixed with PCR reagents and dye, can be used to prepare a plate for thermal and optical High Resolution Melt (HRM) calibration.

TaqMan™ RNase P Instrument Verification Plate for 7300/7500 Systems, 96-well (Applied Biosystems™)

The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems installations. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

NED™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This NED™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL (Applied Biosystems™)

This easy-to-use dye calibration kit establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, SYBR™ , ABY™, JUN™, MUSTANG PURPLE™, TAMRA™, NED™, and Cy®5 dyes on the Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems.

The QuantStudio™ 3/5 10-Dye Spectral Calibration Kit contains the following:
• 96-well 0.2-mL Spectral Calibration Plate 1 (containing FAM, VIC, ROX, and SYBR dyes)
• 96-well 0.2-mL Spectral Calibration Plate 2 (containing ABY, JUN, and MUSTANG PURPLE dyes)
• 96-well 0.2-mL Spectral Calibration Plate 3 (containing TAMRA, NED, and Cy5, dyes)

This multiple-dye plate calibration kit enables simultaneous dye calibrations in single plates, significantly reducing the effort and time required for dye calibration. In addition, the formulations of the dyes in Plate 2 improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes found in your real-time PCR experiments.

Sequence Detection Systems Spectral Calibration Kit, 384-well (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR™ Green, TAMRA™, TET™, and VIC® dyes on the 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 384-Well Calibration kit includes two optical 384-well reaction plates: one preloaded and sealed background plate, and one preloaded and sealed spectral calibration plate with eight separate dye standards.
• 384-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™, and VIC®) are provided for quick and easy calibration of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 384-well block of the 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

7500 Fast Real-Time PCR Systems Spectral Calibration Kit II (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Fast Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three Optical 96-Well Fast Thermocycling Plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

ROI & Background Calibration Kit, Fast 96-well (Applied Biosystems™)

The Fast 96-Well ROI & Background Calibration Kit is used to maintain your real-time PCR instrument with Fast 96-well block. Region of interest (ROI) and background calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

FAM™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.