Shop All Real-Time PCR Instrument Calibration Kits & Reagents

TAMRA™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for TAMRA™ spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ROX™ Dye Calibration Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Dye Calibration Plate (ROX™) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

NED™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for NED™ spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Calibration Install Kit, 384-well (Applied Biosystems™)

This kit is used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 384-well block. It includes calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, and NED™ dyes, as well as plates to perform region-of-interest (ROI) calibration, normalization calibration, and RNase P verification. Calibration and verification should be done at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

FAM™/ROX™ and VIC™/ROX™ Dye Normalization Plates, 384-well (Applied Biosystems™)

This kit is used for normalization calibration of your ViiA™ 7 System with a 384 Well Block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your ViiA™ 7 System with 384-Well Block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7 System will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not for use in diagnostics procedures.

FAM™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

SYBR™ Green Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This SYBR® Green dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

7500 Fast Real-Time PCR Systems Spectral Calibration Kit I (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for FAM™⁄SYBR® Green I, VIC®⁄JOE™, and NED™⁄TAMRA™⁄ROX™ dyes on the 7500 Fast Real-Time PCR System.

The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains nine Optical 96-Well Fast Thermocycling Plates: one preloaded and sealed background plate, seven preloaded and sealed plates with seven separate dye standards, and one region-of-interest (ROI) plate.

• 96-well optical reaction plates preloaded with dyes (FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄TAMRA™⁄ROX™) allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously
• Includes the Passive Reference I dye standard required for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research — Not Instrument Calibration

This kit's easy-to-use, preloaded 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

QuantStudio™ 5 10-Dye Spectral Calibration Kit, 384-well (Applied Biosystems™)

This easy-to-use dye calibration kit establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, TAMRA™, SYBR™ , ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy®5 dyes on the Applied Biosystems™ QuantStudio™ 5 384-well real-time PCR system.

The QuantStudio™ 5 10-Dye Spectral Calibration Kit contains the following:

• QuantStudio™ 5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, TAMRA™, and SYBR™ dyes), 384-well (Cat. No. A26334)
• QuantStudio™ 5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™, NED™, and Cy®5 dyes), 384-well (Cat. No. A26335)

This multiple-dye plate calibration kit enables simultaneous dye calibrations in single plates, significantly reducing the effort and time required for dye calibration. In addition, the formulations of the dyes in Plate 2 improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes found in your real-time PCR experiments.

MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ViiA™ 7 Array Card RNaseP Verification Kit (Applied Biosystems™)

The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® ViiA™ 7 Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade.

The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit contains an empty TaqMan® Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

The ViiA™ 7 System TaqMan® Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TaqMan™ RNase P Instrument Verification Plate for ViiA™ 7 System, 96-well (for 0.2 mL block) (Applied Biosystems™)

The ViiA™ 7 System 96-Well Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® ViiA™ 7 Real-Time PCR Systems with the 96-Well Block.

The ViiA™ 7 System 96-Well Instrument Verification RNase P kit contains a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

The ViiA™ 7 System 96-Well Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

Region of Interest (ROI) and Background Plates, Fast 96-well (Applied Biosystems™)

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with Fast 96-well (0.1 mL) blocks. See the instrument maintenance guide for full instructions on use.

FAM™ Dye Calibration Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Dye Calibration Plate (FAM™) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

ViiA™ 7 Array Card Spectral Calibration Kit (Applied Biosystems™)

This kit is used to perform spectral calibration, region-of-interest (ROI) calibration, and normalization on your ViiA™ 7, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with array card block. It includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC® dye, ROX™ dye, ROI dye mix, background buffer, FAM™⁄ROX™ dye, VIC®⁄ROX™ dye. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

VIC™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This VIC® dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Region of Interest (ROI) and Background Plates, 96-well (Applied Biosystems™)

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 96-well (0.2 mL) blocks. See the instrument maintenance guide for full instructions on use.

Calibration Kit, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Calibration Kit is used to maintain your real-time PCR instrument with Fast 96-well block. The kit includes region of interest (ROI), background, and dye calibrations, as well as RNaseP verification. Please see your instrument user guide for full instructions on use.

TAMRA™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This TAMRA™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 1 (FAM™, VIC™, ROX™, SYBR™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, and SYBR™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.1 mL (Applied Biosystems™)

This easy-to-use dye calibration kit establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, SYBR™ , ABY™, JUN™, MUSTANG PURPLE™, TAMRA™, NED™, and Cy®5 dyes on the Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems.

The QuantStudio™ 3/5 10-Dye Spectral Calibration Kit contains the following:
• 96-well 0.1-mL Spectral Calibration Plate 1 (containing FAM, VIC, ROX, and SYBR dyes)
• 96-well 0.1-mL Spectral Calibration Plate 2 (containing ABY, JUN, and MUSTANG PURPLE dyes)
• 96-well 0.1-mL Spectral Calibration Plate 3 (containing TAMRA, NED, and Cy5, dyes)

This multiple-dye plate calibration kit enables simultaneous dye calibrations in single plates, significantly reducing the effort and time required for dye calibration. In addition, the formulations of the dyes in Plate 2 improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes found in your real-time PCR experiments.

7500 Real Time PCR Systems Spectral Calibration Kit II (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three optical 96-well reaction plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

TAMRA™ Dye Calibration Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Dye Calibration Plate (TAMRA™) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

7500 Real Time PCR Systems Spectral Calibration Kit I (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains one background plate, one region of interest (ROI) calibration plate, and seven spectral calibration plates with seven separate dye standards.

• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

7900HT System Spectral Calibration Kit, Fast 96-well (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, TAMRA™, TET™, and VIC® dyes on the 7900HT Fast Real-Time PCR System. The Applied Biosystems® 7900 HT System Fast 96-Well Spectral Calibration Kit contains three Optical 96-Well Fast Thermocycling Plates: one preloaded and sealed background plate and two preloaded and sealed with eight separate dye standards.

• 96-well optical reaction plates preloaded with FAM™, JOE™, NED™, ROX™, SYBR® Green, TAMRA™, TET™, and VIC® dyes allow quick and easy calibration of the Applied Biosystems® 7900HT Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously
• Includes the Passive Reference I dye standard required for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7900HT Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

FAM™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for FAM™ spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-Well Block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TaqMan™ RNase P Instrument Verification Plate for ViiA™ 7 System, 384-well (Applied Biosystems™)

Used to perform verification of your ViiA™7 System with 384 Well Block. Please see your instrument maintenance guide for full instructions on use.

QuantStudio™ 12K Flex OpenArray™ Block RNase P Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Block Rnase P Kit is used for verification of the QuantStudio™ 12K Flex OpenArray® block. The kit contains 2 Rnase P arrays plus reagents Each kit also comes with sufficient accessories to perform two experiments: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TaqMan™ RNase P Instrument Verification Plate for 7300/7500 Systems, 96-well (Applied Biosystems™)

The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems installations. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

TaqMan™ RNase P Instrument Verification Plate for 7900HT System, 384-well (Applied Biosystems™)

The Applied Biosystems® TaqMan® RNase P 384-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR System. The TaqMan® RNase P 384-Well Instrument Verification Plate is a 384-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template.
The TaqMan® RNase P Instrument 384-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7900 HT Real-Time PCR Systems installation. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

QuantStudio™ 3/5 Spectral Calibration Plate 3 (TAMRA™, NED™, Cy™5 dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for TAMRA™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

TaqMan™ Low Density Array Spectral Calibration Kit (Applied Biosystems™)

The TaqMan® Low Density Array Spectral Calibration Kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The TaqMan® Low Density Array Spectral Calibration Kit contains four empty TaqMan® Low Density Array, one background tube, and three separate pure dye standards (FAM™, ROX™,VIC®). Insert the loaded calibration arrays during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis. To ensure accurate results, we recommend that the performance of your instrument be verified every six months.

FAM™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This FAM™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

MeltDoctor™ HRM Calibration Plate, 384-well (Applied Biosystems™)

Applied Biosystem® ready-to-use MeltDoctor™ HRM Calibration Plates contain all the components required for pure dye and HRM calibration, reducing the complexity of getting started with HRM experimentation. These plates provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves.

FAM™/ROX™ and VIC™/ROX™ Dye Normalization Plates, Fast 96-well (Applied Biosystems™)

These normalization plates can be used to maintain your ViiA&trade 7 System with Fast 96-Well Block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7 System will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

SYBR™ Green Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for SYBR® spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

VIC™ Dye Calibration Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Dye Calibration Plate (VIC®) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

Region of Interest (ROI) and Background Plates, 384-well (Applied Biosystems™)

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with 384-well blocks. See the instrument maintenance guide for full instructions on use.

ROX™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This ROX™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 3/5 Spectral Calibration Plate 3 (TAMRA™, NED™, Cy™5 dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for TAMRA™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Array Card Calibration Install Kit (Applied Biosystems™)

This kit is used to maintain your ViiA™ 7 System with TaqMan® Array Card Block. It includes reagents to perform a spectral calibration with FAM™, VIC®, and ROX™ dyes, region-of-interest (ROI) and normalization calibration, and to run an RNase P verification card. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7 System will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

7500 Fast Real-Time PCR Systems Spectral Calibration Kit II (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Fast Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three Optical 96-Well Fast Thermocycling Plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

Normalization Plates with FAM™/ROX™ and VIC™/ROX™ dyes, 96-well (Applied Biosystems™)

These normalization plates can be used to maintain your ViiA&trade 7 System with 96-Well Block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7 System will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

NED™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This NED™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

NED™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This NED™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Calibration Install Kit, Fast 96-well (Applied Biosystems™)

This kit is used to maintain your ViiA 7, QuantStudio 6 Flex, QuantStudio 7 Flex, and QuantStudio 12k Flex real-time PCR systems with Fast 96-well block. It includes calibration plates to perform a spectral calibration with FAM, VIC, ROX, SYBR Green, TAMRA, NED dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

RNaseP Verification Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well RNaseP Verification Plate is used to verify your real-time PCR instrument with Fast 96-Well Block. Confirm the performance of your instrument by running the RNaseP assay. Please see your instrument user guide for full instructions on use.

The Fast 96-Well RNaseP Verification Plate is preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

The verification plate must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, it is recommended that the performance of your instrument be verified every six months.

7900HT TaqMan™ Low Density Array Instrument Verification RNase P Kit (Applied Biosystems™)

The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit contains an empty TaqMan® Low Density Array and 8 vials of RNase P detection reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each vial contains premixed reagents (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7900HT TaqMan® Low Density Array Instrument Verification RNase P kit must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

TaqMan™ RNase P Instrument Verification Plate, Fast 48-well (Applied Biosystems™)

Applied Biosystems® TaqMan® RNase P Fast 48-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® StepOne™ Real-Time PCR System. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate is a 48-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The TaqMan® RNase P Fast 48-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® StepOne™ Real-Time PCR Systems installation. To pass installation, the instrument must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

Sequence Detection Systems Spectral Calibration Kit, 96-well (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR® Green, TAMRA™, TET™, and VIC™ dyes on the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 96-Well Calibration kit includes three optical 96-well reaction plates: one preloaded and sealed background plate, and two preloaded and sealed spectral calibration plates with eight separate dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™,ROX™, SYBR® Green , TAMRA™, TET™, and VIC™) are provided for quick and easy calibration of the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the standard 96-well block of the ABI PRISM® 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

QuantStudio™ 3/5 10-Dye Spectral Calibration Kit, 96-well, 0.2 mL (Applied Biosystems™)

This easy-to-use dye calibration kit establishes the pure dye spectra and multicomponent values for FAM™, VIC™, ROX™, SYBR™ , ABY™, JUN™, MUSTANG PURPLE™, TAMRA™, NED™, and Cy®5 dyes on the Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems.

The QuantStudio™ 3/5 10-Dye Spectral Calibration Kit contains the following:
• 96-well 0.2-mL Spectral Calibration Plate 1 (containing FAM, VIC, ROX, and SYBR dyes)
• 96-well 0.2-mL Spectral Calibration Plate 2 (containing ABY, JUN, and MUSTANG PURPLE dyes)
• 96-well 0.2-mL Spectral Calibration Plate 3 (containing TAMRA, NED, and Cy5, dyes)

This multiple-dye plate calibration kit enables simultaneous dye calibrations in single plates, significantly reducing the effort and time required for dye calibration. In addition, the formulations of the dyes in Plate 2 improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes found in your real-time PCR experiments.

ROX™ Dye Spectral Calibration Plate, 384-well (Applied Biosystems™)

This kit is used for ROX™ spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with a 384-well block. Please see your instrument maintenance guide for full instructions on use.

These calibration kits are used to maintain your real-time PCR system with 384-well block. They include calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, NED™ dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

QuantStudio™ 12K Flex OpenArray™ Calibration Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Calibration Kit is offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray® block type. The calibration kit contains FAM™ dye, as well as a calibration starter kit. The calibration starter kit contains the calibration plaque for background and uniformity, as well as four empty cases for dye loading. Calibration is recommended on a yearly basis. Please see your instrument maintenance guide for full instructions on use.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.