Shop All Real-Time PCR Instrument Calibration Kits & Reagents

TAMRA™ Dye Calibration Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well Dye Calibration Plate (TAMRA™) is used to maintain your real-time PCR instrument with Fast 96-well block. Dye calibration should be run after lamp change. Please see your instrument user guide for full instructions on use.

ROX™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This ROX™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

7500 Fast Real-Time PCR Systems Spectral Calibration Kit I (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for FAM™⁄SYBR® Green I, VIC®⁄JOE™, and NED™⁄TAMRA™⁄ROX™ dyes on the 7500 Fast Real-Time PCR System.

The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit I contains nine Optical 96-Well Fast Thermocycling Plates: one preloaded and sealed background plate, seven preloaded and sealed plates with seven separate dye standards, and one region-of-interest (ROI) plate.

• 96-well optical reaction plates preloaded with dyes (FAM™⁄SYBR® Green I, VIC®⁄JOE™, NED™⁄TAMRA™⁄ROX™) allow quick and easy calibration of the 7500 Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously
• Includes the Passive Reference I dye standard required for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research — Not Instrument Calibration

This kit's easy-to-use, preloaded 96-well optical reaction plates enable quick and easy calibration of the 7500 Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

TaqMan™ RNase P Instrument Verification Plate for ViiA™ 7 System, 384-well (Applied Biosystems™)

Used to perform verification of your ViiA™7 System with 384 Well Block. Please see your instrument maintenance guide for full instructions on use.

MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

SDS Spectral Calibration Kit (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™ dyes on the ABI PRISM 7700® Sequence Detection System.

The SDS Spectral Calibration kit includes 8 tubes of pure dye standards for the Applied Biosystems® ABI PRISM 7700® Sequence Detection System.

• Individual tubes containing eight dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™) are provided for quick and easy calibration of the ABI PRISM 7700® Sequence Detection System
• Individual components provide flexibility in dye standards setup



• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration


This kit enables users to calibrate the ABI PRISM 7700® Sequence Detection System quickly and easily. The system automatically collects the dye standard's spectral information as part of the instrument installation procedure. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.1 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.1-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

QuantStudio™ 12K Flex OpenArray™ Calibration Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Calibration Kit is offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray® block type. The calibration kit contains FAM™ dye, as well as a calibration starter kit. The calibration starter kit contains the calibration plaque for background and uniformity, as well as four empty cases for dye loading. Calibration is recommended on a yearly basis. Please see your instrument maintenance guide for full instructions on use.

StepOnePlus™ Real-Time PCR System Spectral Calibration Kit (96 well) (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™/SYBR® Green I, VIC®/JOE™, and NED™/TAMRA™/ROX™ dyes on the StepOnePlus™ Real-Time PCR System.

The Applied Biosystems® StepOnePlus™ Real-Time PCR System Spectral Calibration Kit contains one background plate and two preloaded, 96-well fast optical reaction plates containing seven dye standards.
• 96-well optical reaction plates preloaded with dyes (FAM™/SYBR® Green I, VIC®/JOE™, NED™/TAMRA™/ROX™) are provided for quick and easy calibration of the StepOnePlus™ Real-Time PCR System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration
This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the StepOnePlus™ Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

7500 Real Time PCR Systems Spectral Calibration Kit II (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three optical 96-well reaction plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

ViiA™ 7 Array Card Spectral Calibration Kit (Applied Biosystems™)

This kit is used to perform spectral calibration, region-of-interest (ROI) calibration, and normalization on your ViiA™ 7, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with array card block. It includes empty array cards and seven tubes of calibration dyes including: FAM™ dye, VIC® dye, ROX™ dye, ROI dye mix, background buffer, FAM™⁄ROX™ dye, VIC®⁄ROX™ dye. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your ViiA™ 7, QuantStudio™ 7, or QuantStudio™ 12K Flex system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

MeltDoctor™ HRM Calibration Standard (20X) (Applied Biosystems™)

Applied Biosystems® MeltDoctor™ HRM Calibration Standard contains a DNA template and primers which, when mixed with PCR reagents and dye, can be used to prepare a plate for thermal and optical High Resolution Melt (HRM) calibration.

7900HT System Spectral Calibration Kit, Fast 96-well (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR® Green, TAMRA™, TET™, and VIC® dyes on the 7900HT Fast Real-Time PCR System. The Applied Biosystems® 7900 HT System Fast 96-Well Spectral Calibration Kit contains three Optical 96-Well Fast Thermocycling Plates: one preloaded and sealed background plate and two preloaded and sealed with eight separate dye standards.

• 96-well optical reaction plates preloaded with FAM™, JOE™, NED™, ROX™, SYBR® Green, TAMRA™, TET™, and VIC® dyes allow quick and easy calibration of the Applied Biosystems® 7900HT Fast Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously
• Includes the Passive Reference I dye standard required for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable quick and easy calibration of the 7900HT Fast Real-Time PCR System. Insert the reaction plates during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ABY™ Dye Spectral Calibration Plate, 96-well (Applied Biosystems™)

This plate is used for ABY® spectral calibration of your Applied Biosystems® 7500 or ViiA™ 7 real-time PCR system with a 96-well block. Please see your instrument maintenance guide for full instructions on use. The dye formulations will help improve multiplexing results by more accurately representing fluorescent spectra that are part of your real-time PCR experiments.