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Pierce™ Intact Protein Standard Mix (Thermo Scientific™)

The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as a quality control standard for LC and LC/MS, LC/MS method development, and intact protein mass measurements.

Verified protein sequences—protein sequences confirmed by MS with the average and monoisotopic masses corresponding to each provided sequence
Recombinant—proteins expressed in E. coli and B. subtilis; no bovine sources
Compatible—protein mixture designed for QC over a wide range of molecular weights (10-70 kDa) and m/z distribution (600-1500 m/z)
Flexible—for use in LC, LC-MS, and direct infusion methods
Stable—provided in a stable lyophilized format

The Pierce Intact Protein Standard Mix contains a mixture of six highly pure recombinant proteins, including human IGF-1 LR3, human thioredoxin, Streptococcus dysgalactiae Protein G, Bovine Carbonic Anhydrase II, Streptococcus Protein AG (chimeric), and Escherichia coli Exo Klenow, expressed in bacteria. These proteins were selected based on their wide range of molecular weights, m/z distribution, and MS fragmentation patterns. After expression and purification, proteins are optimally mixed and lyophilized for stability and convenience. Each vial contains 76 µg of lyophilized protein mixture and does not contain salts or detergents.

Applications:
• Qualitative LC
• LC/MS standardization and method development
• LC/MS intact protein mass measurement and top-down method development
• Digestion protocol optimization

Protein sequences for Pierce Intact Protein Mix components (FASTA format) ›

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Pierce Peptide Retention Time Calibration Mixture
Pierce 6 Protein Digest, equimolar, LC-MS grade
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Pierce™ Peptide Retention Time Calibration Mixture (Thermo Scientific™)

The Thermo Scientific Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

Features of Peptide Retention Time Calibration Mixture:

• Assess chromatography and MS instrument performance
• Predict peptide retention across multiple instrument platforms
• Predict peptide retention time from sequence using calculated hydrophobicity factor
• Optimize scheduled MS acquisition windows for improved quantification and increased multiplexing
• Normalize collision energies between instruments and platforms
• Normalize results for variation in retention times and peak intensities between runs

The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify the total peptide elution window, and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture can predict peptide retention times based upon peptide sequence or relative retention time between instruments. The mixture can also be used with Pinpoint software to establish normalized collision energies between platforms. This allows normalization of methods and eases the transfer of quantitative methods between LC-MS platforms. Pinpoint software, sample files, tutorials, and videos can be downloaded from the Thermo Proteomics Portal.

The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HF) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time.

The prediction of peptide retention time assists in the development of multiplexed, high throughput mass spectrometric assays. The Pierce Peptide Retention Time Calibration Mixture and Thermo Scientific Pinpoint software streamline the transition from qualitative protein discovery results to the development of targeted MS assays on Thermo Scientific triple quadrupole, Orbitrap, Exactive, and ion trap mass spectrometers. The Pierce Peptide Retention Time Calibration Mixture permits optimization and regular assessment of chromatographic performance and for rapid development of multiplexed, scheduled targeted MS assays for the quantification of dozens to hundreds of peptide targets per run.

More Product Data
Optimize liquid chromatography with a peptide retention time calibration mixture

Peptide Retention Standard (Thermo Scientific™)

Thermo Scientific Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC.

Features of Peptide Retention Standard:

• Determine the relative order of peptide elution from a complex mixture
• Efficiently predict peptide elution profiles to simplify identification and save purification time
• Identify peptides of known amino acid composition on reverse phase HPLC columns
• Monitor column performance—efficiency, selectivity and resolution during column aging
• Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities
• Compare reverse-phase columns from different manufacturers

Peptide Retention Standard allows accurate prediction of elution time for peptides of known amino acid composition up to 20 residues in length. The reference standard helps to predict the relative order of peptide elution within a complex mixture, simplifying identification of specified peptides and saving time in peptide purification. By testing the standard frequently throughout the lifetime of a column, it is possible to monitor performance characteristics such as efficiency, selectivity, and resolution during column aging. The standard can also be used to evaluate different reverse phase supports of varying n-alkyl chain lengths and ligand density, as well as columns from different manufacturers.

The Peptide Retention Standard consists of five C-terminal amide decapeptides, denoted S1 to S5, having the generic formula Ac-Arg-Gly-X-X-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Four of these peptides are Na-acetylated with the sequence variation as follows: Gly3-Gly4, Ala3-Gly4, Val3-Gly4 and Val3-Val4. The fifth peptide, Ala3-Gly4, contains a free Na-amino group. This mixture will provide 100-200 injections (approximately 0.1 mg of each peptide) at 0.1 AUFS at 210nm. The Peptide Retention Standard is supplied as a dry film in a sealed vial.

A simple, quantitative method for predicting peptide retention times was developed by Guo, et al. (1985, 1986). Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups.

Retention time is dependent upon the molecular weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule. The accuracy of predicting peptide retention time significantly decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density.

Pierce™ Peptide Retention Time Calibration Mixture (Thermo Scientific™)

The Thermo Scientific Pierce Peptide Retention Time Calibration Mixture enables reversed phase liquid chromatography (LC) and mass spectrometry (LC-MS) users to optimize and confirm correct operation of their equipment.

Features of Peptide Retention Time Calibration Mixture:

• Assess chromatography and MS instrument performance
• Predict peptide retention across multiple instrument platforms
• Predict peptide retention time from sequence using calculated hydrophobicity factor
• Optimize scheduled MS acquisition windows for improved quantification and increased multiplexing
• Normalize collision energies between instruments and platforms
• Normalize results for variation in retention times and peak intensities between runs

The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify the total peptide elution window, and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture can predict peptide retention times based upon peptide sequence or relative retention time between instruments. The mixture can also be used with Pinpoint software to establish normalized collision energies between platforms. This allows normalization of methods and eases the transfer of quantitative methods between LC-MS platforms. Pinpoint software, sample files, tutorials, and videos can be downloaded from the Thermo Proteomics Portal.

The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HF) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time.

The prediction of peptide retention time assists in the development of multiplexed, high throughput mass spectrometric assays. The Pierce Peptide Retention Time Calibration Mixture and Thermo Scientific Pinpoint software streamline the transition from qualitative protein discovery results to the development of targeted MS assays on Thermo Scientific triple quadrupole, Orbitrap, Exactive, and ion trap mass spectrometers. The Pierce Peptide Retention Time Calibration Mixture permits optimization and regular assessment of chromatographic performance and for rapid development of multiplexed, scheduled targeted MS assays for the quantification of dozens to hundreds of peptide targets per run.

More Product Data
Optimize liquid chromatography with a peptide retention time calibration mixture

CHCA MALDI Matrix, Single-Use (Thermo Scientific™)

The Thermo Scientific CHCA MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

Features of CHCA MALDI Matrices:

• Single-use packaging saves time and reduces waste
• Provides accurate quantity of matrix every time
• Applications: peptides, lipids, nucleotides

Matrix assisted laser desorption ionization (MALDI) matrices produce the cleanest mass spectrometric (MS) spectra when they are recrystallized and prepared daily, which is inconvenient and typically results in significant waste. The unique packaging of purified, recrystallized alpha-cyano-4-hydroxy-cinnamic acid (CHCA) MALDI matrices makes it easy to prepare high-quality MALDI reagents in minutes.

MALDI and electrospray ionization (ESI) are the most common methods to ionize peptides for MS analysis. MALDI is widely used because it is significantly faster than ESI and the ionized molecules are almost exclusively singly charged, making interpretation easier. Also, MALDI is readily automated and highly sensitive. This powerful method for MS peptide analysis is now easier to perform with our single-use, highly pure MALDI matrices that are exceptionally convenient to use.

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MALDI Matrix Sample Pack, Single-Use
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Pierce™ LTQ ESI Positive Ion Calibration Solution (Thermo Scientific™)

The Thermo Scientific Pierce LTQ ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules specifically designed for positive mode calibration of Thermo Scientific ion trap and orbitrap instruments.

Features of LTQ ESI Positive Ion Calibration Solution:

Strong peaks—mixture of caffeine, MRFA and Ultramark 1621 in a solution of acetonitrile, methanol, and acetic acid
Ready-to-use—load the mass reference standard into a syringe and inject into the instrument
High-purity—mass spectrometry grade reagents in a non-leachable container

The Pierce LTQ ESI Positive Ion Calibration Solution is a ready-to-use liquid formulation ideal for quickly performing the routine calibration required to maintain the robust performance of Thermo Scientific Mass Spectrometers. The LTQ ESI Positive Ion Calibration Solution is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solution is provided in a leak-proof, high-purity PTFE bottle.

The LTQ ESI Positive Ion Calibration Solution can be used to calibrate the LTQ series, the LTQ Orbitrap series, the LXQ, LCQ FLEET and the Exactive Mass Spectrometer instruments.

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Calibration solutions for Thermo Scientific Mass Spectrometers

Pierce™ Negative Ion Calibration Solution (Thermo Scientific™)

The Thermo Scientific Pierce ESI Negative Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for negative mode calibration of Thermo Scientific LTQ series mass spectrometer instruments.

Features of the Negative Ion Calibration Solution:

Strong peaks—mixture of SDS, sodium taurocholate and Ultramark 1621 in a acetonitrile/methanol/acetic solution.
Ready-to-use—load the mass reference standard into a syringe and inject into the instrument
High-purity—mass spectrometry grade reagents in a non-leachable container
Stable—store at room temperature for more than one year

The Pierce ESI Negative Ion Calibration Solution is a ready-to-use liquid formulation ideal for quickly performing the routine calibration required to maintain the robust performance of Thermo Scientific Mass Spectrometers. The ESI Negative Ion Calibration Solution is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solution is provided in a leak-proof, high-purity PTFE bottle.

The ESI Negative Ion Calibration Solution can be used to calibrate the LTQ series, LTQ Velos series, the LTQ Orbitrap series and Exactive Mass Spectrometer instruments.

More Product Data
Calibration solutions for Thermo Scientific Mass Spectrometers

Pierce™ BSA Protein Digest, MS grade (Thermo Scientific™)

The Thermo Scientific™ Pierce™ BSA Protein Digest Standard, LC-MS Grade, is a validated protein digest optimized for use as a quality control sample for liquid chromatography (LC) and mass spectrometry (MS) analysis of proteomic samples.

Positive control sample—BSA digest optimized and validated as a quality control standard for mass spectrometry applications
Excellent sequence coverage—greater than 85% sequence coverage
Validated peptide quality—digestion procedure optimized for minimal missed cleavages and over-alkylation
Rigorously tested—high-quality, consistent BSA protein digest documented via lot-specific certificates of analysis
Stable—provided in a stable lyophilized format

The Pierce BSA Protein Digest Standard is produced using high quality bovine serum albumin (BSA) and mass spectrometry-grade trypsin. The BSA is fully reduced, alkylated (iodoacetamide), and desalted (C18) after trypsin digestion to provide a quality LC/MS-grade standard that is free of intact protein. The peptide digest standard provides excellent sequence coverage with minimal over-alkylation and missed cleavages. This digest standard has been robustly tested for peptide quality, digestion efficiency, and lot-to-lot uniformity and is suitable for LC and LC-MS applications.

SA MALDI Matrix, Single-Use (Thermo Scientific™)

The Thermo Scientific SA MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

Features of SA MALDI Matrices:

• Single-use packaging saves time and reduces waste
• Provides accurate quantity of matrix every time
• Applications: large peptides, whole proteins, lipids

Matrix assisted laser desorption ionization (MALDI) matrices produce the cleanest mass spectrometric (MS) spectra when they are recrystallized and prepared daily, which is inconvenient and typically results in significant waste. The unique packaging of purified, recrystallized sinapinic acid (SA) MALDI matrices makes it easy to prepare high-quality MALDI reagents in minutes.

MALDI and electrospray ionization (ESI) are the most common methods to ionize peptides for MS analysis. MALDI is widely used because it is significantly faster than ESI and the ionized molecules are almost exclusively singly charged, making interpretation easier. Also, MALDI is readily automated and highly sensitive. This powerful method for MS peptide analysis is now easier to perform with our single-use, highly pure MALDI matrices that are exceptionally convenient to use.

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MALDI Matrix Sample Pack, Single-Use
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Pierce™ FlexMix™ Calibration Solution (Thermo Scientific™)

The Thermo Scientific Pierce FlexMix Calibration Solution is a mixture of 16 highly pure, ionizable components (mass ranges: 50 to 3000 m/z) designed for both positive and negative ionization calibration using Orbitrap Tribrid Series 3.1 Instrument Control Software (or later) for Orbitrap Fusion, Orbitrap Fusion Lumos, and Orbitrap ID-X MS instruments.

Pierce FlexMix Calibration Solution features include:
Improved mass accuracy—extended mass range improves mass accuracy
Positive and negative mode calibration—single formulation with extended mass ranges for both ionization calibration modes
Highly validated—high-quality, rigorously-tested formulation with lot-specific certificates of analysis
Ready-to-use—reference standard may be directly loaded into a syringe and injected into the instrument
Stable—store at room temperature for up to 18 months

The Pierce FlexMix Calibration Solution is a ready-to-use formulation that has been optimized for both positive and negative ionization calibration of instruments. The extended range masses (50 to 3000 m/z) improve sensitivity and mass accuracy of Orbitrap mass spectrometers. Additionally, the FlexMix Calibration Solution is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solution is provided in a leak-proof, high-purity PTFE bottle.

For the calibration procedure, use the Orbitrap Tribrid Series Getting Started Guide.

Related products:
Pierce HeLa Protein Digest Standard
Pierce TMT11plex Yeast Digest Standard

Pierce™ Reserpine Standard for LC-MS (Thermo Scientific™)

Thermo Scientific Pierce Reserpine Standard for LC-MS is a precise concentration of reserpine specifically designed for performance evaluation of mass spectrometers, including the Thermo Scientific ion trap and TSQ series of instruments.

Features of Reserpine Standard for LC-MS:

Convenient—provided pre-diluted and requires minimal additional dilutions to reach target concentration for injection
Safe handling—unlike glass ampules, cap can be easily removed and replaced when withdrawing solution
High-purity—mass spectrometry grade reagent in a non-leachable screw-cap vials
Stable—store at 4°C for up to one year

Pierce Reserpine Standard for LC-MS is a pre-diluted liquid formulation ideal to quickly perform installation specification tests of Thermo Scientific and other manufacturers' mass spectrometers. The standard is provided in a concentration of 100pg/µL in 50% isopropyl alcohol and requires minimal additional dilutions. The product is provided in a pack of 5 x 1 mL glass amber vials with PTFE-lined screw caps. Pierce Reserpine Standard for LC-MS is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications.

Pierce Reserpine Standard for LC-MS can be used to assess performance of mass spectrometers, including the Thermo Scientific ion trap series and the TSQ series.

Pierce™ TMT11plex Yeast Digest Standard (Thermo Scientific™)

The Thermo Scientific Pierce TMT11plex Yeast Digest Standard is a lyophilized yeast peptide mixture of four congenic strains labeled with TMT11plex reagents to monitor LC-MS/MS system performance for Tandem Mass Tag (TMT) quantitation.

Pierce TMT11plex Yeast Digest Standard features include:
Convenient—pre-labeled with TMT11plex reagents in a ready-to-use lyophilized format
Highly validated—yeast strains verified for genotype, equimolar mixing of the TMT-labeled strains, and overall protein quantitation using TMT
Diagnostic—excellent QC assay tool for quality assessment of LC and MS instrument status
Quantitative—designed to measure the quantitative precision of the TMT reporter ions for each of the 11 channels

Quantitative proteomic strategies using TMT reagents enable sample multiplexing and precise measurement of protein abundance. However, successful execution of this workflow includes multiple steps that may require optimization, including chromatography, mass spectrometry (MS), and data analysis. A critical parameter for success in this workflow is the ability to consistently detect the TMT fragment ions across the 11 channels of multiplexing. This standard has been specifically designed as a tool to monitor LC-MS/MS system performance for TMT quantitation and MS method optimization and validation.

The Pierce TMT11plex Yeast Digest Standard consists of four congenic strains of Saccharomyces cerevisiae (a parental line, BY4741, and three lines lacking the non-essential proteins Met6, His4, or Ura2) labeled in triplicate (knock-out strains) or duplicate (parental strain) with TMT11-plex label reagents (see figure). The knock-out strains are used in combination with the parental strain to enable comparison of peptide and protein abundance in the deleted and parental strains to monitor system performance for quantitation.

In addition to routine quality control assessment, the standard can also be used as a method development tool to measure the accuracy, precision, and dynamic range of different MS approaches, including optimization of offline fractionation, chromatography, MS acquisition, or data analysis settings.

The multifunctional applications for this standard will allow more reproducible TMT quantitation from day-to-day and experiment-to-experiment.

References:
1. Paulo JA, O'Connell JD, Gygi SP. A Triple Knockout (TKO) Proteomics Standard for Diagnosing Ion Interference in Isobaric Labeling Experiments. J Am Soc Mass Spectrom. 2016 10:1620-5.
2. Yang F, Shen Y, Camp DG 2nd, Smith RD. High-pH reversed-phase chromatography with fraction concatenation for 2D proteomic analysis. Expert Rev Proteomics. 2012 9(2):129-34.
3. Dayon L, Pasquarello C, Hoogland C, Sanchez JC, Scherl A. Combining low- and high-energy tandem mass spectra for optimized peptide quantification with isobaric tags. J Proteomics. 2010 73(4):769-77.
4. Diedrich JK, Pinto AF, Yates JR 3rd. Energy dependence of HCD on peptide fragmentation: stepped collisional energy finds the sweet spot. J Am Soc Mass Spectrom. 2013 24(11):1690-9.
5. Chiva C, Sabidó E. HCD-only fragmentation method balances peptide identification and quantitation of TMT-labeled samples in hybrid linear ion trap/orbitrap mass spectrometers. J Proteomics. 2014 96:263-70.

Related products:
Pierce HeLa Protein Digest Standard
Pierce Peptide Retention Time Calibration Mixture
TMT10plex isobaric label reagent set plus TMT11-131C label reagent

DHB MALDI Matrix, Single-Use (Thermo Scientific™)

The Thermo Scientific DHB MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

Features of DHB MALDI Matrices:

• Single-use packaging saves time and reduces waste
• Provides accurate quantity of matrix every time
• Applications: small molecules, small peptides, nucleotides, oligonucleotides, oligosaccharides

Matrix assisted laser desorption ionization (MALDI) matrices produce the cleanest mass spectrometric (MS) spectra when they are recrystallized and prepared daily, which is inconvenient and typically results in significant waste. The unique packaging of purified, recrystallized 2,5-dihydroxybenzoic acid (DHB) MALDI matrices makes it easy to prepare high-quality MALDI reagents in minutes.

MALDI and electrospray ionization (ESI) are the most common methods to ionize peptides for MS analysis. MALDI is widely used because it is significantly faster than ESI and the ionized molecules are almost exclusively singly charged, making interpretation easier. Also, MALDI is readily automated and highly sensitive. This powerful method for MS peptide analysis is now easier to perform with our single-use, highly pure MALDI matrices that are exceptionally convenient to use.

Related Products
MALDI Matrix Sample Pack, Single-Use
CHCA MALDI Matrix, Single-Use
SA MALDI Matrix, Single-Use

Pierce™ LTQ Velos ESI Positive Ion Calibration Solution (Thermo Scientific™)

The Thermo Scientific Pierce LTQ Velos ESI Positive Ion Calibration Solution is a mixture of highly purified ionizable molecules designed for positive mode calibration of Thermo Scientific LTQ Velos series mass spectrometer instruments.

Features of LTQ Velos ESI Positive Ion Calibration Solution:

Strong peaks—mixture of caffeine, MRFA, Ultramark 1621 and n-butylamine in a acetonitrile/methanol/acetic solution.
Ready-to-use—load the mass reference standard into a syringe and inject into the instrument
High-purity—mass spectrometry grade reagents in a non-leachable container
Stable—store at room temperature for more than one year

The Pierce LTQ Velos ESI Positive Ion Calibration Solution is a ready-to-use liquid formulation ideal for quickly performing the routine calibration required to maintain the robust performance of Thermo Scientific Mass Spectrometers. The LTQ Velos ESI Positive Ion Calibration Solution is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solution is provided in a leak-proof, high-purity PTFE bottle.

The LTQ Velos ESI Positive Ion Calibration Solution can be used to calibrate the LTQ Velos/Velos Pro, the LTQ Orbitrap Velos/Orbitrap Velos Pro, the Orbitrap Elite, and the QExactive mass spectrometer instruments.

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Calibration solutions for Thermo Scientific Mass Spectrometers

β-Lactoglobulin (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.