Shop All Instrument Calibration Kits, Standards & Reagents

TaqMan™ RNase P Instrument Verification Plate for 7900HT System, 384-well (Applied Biosystems™)

The Applied Biosystems® TaqMan® RNase P 384-Well Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR System. The TaqMan® RNase P 384-Well Instrument Verification Plate is a 384-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template.
The TaqMan® RNase P Instrument 384-Well Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7900 HT Real-Time PCR Systems installation. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

QuantStudio™ 12K Flex OpenArray™ Block RNase P Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Block Rnase P Kit is used for verification of the QuantStudio™ 12K Flex OpenArray® block. The kit contains 2 Rnase P arrays plus reagents Each kit also comes with sufficient accessories to perform two experiments: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™ System tips, and 384-well plates.

MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

DS-30 Matrix Standard Kit (Dye Set D) (Applied Biosystems™)

The DS-30 Matrix Standard kit contains 4 oligonucleotides respectively labeled with 6-FAM™, HEX™, NED™, and ROX™, pre-pooled in a single tube. This Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing fluorescent dye-labeled DNA fragments on the Applied Biosystems® 3100, 3130, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary.

For Research Use Only. Not for use in diagnostics procedures.

Instrument Control Terbium TR-FRET Kit

The Instrument Control Terbium TR-FRET Kit allows for the rapid assessment of instrument setup for terbium (Tb)-based TR-FRET assays. LanthaScreen® and Premo™ assays require specific instrument settings. Proper instrument setup is critical to experimental success. This kit contains one tube each of 1x high and low controls which may also be used as positive control samples when running Tb TR-FRET assays.

The Instrument Control Terbium TR-FRET Kit Allows You to:

• Set up instruments for Tb TR-FRET assays quickly
• Check instrument Tb TR-FRET settings
• Read a high and low Tb TR-FRET signal control on each assay plate

Determine if Your Instrument is Set Up to Read Tb TR-FRET Assays
Setting up your instrument to read Tb TR-FRET is the first step in running a successful Tb TR-FRET assay. This kit allows you to quickly determine if your instrument is set up properly to read Tb TR-FRET assays. Simply add a small volume into separate wells, place the plate into the instrument and read. If the ratio of high to low control signal is below 2 the instrument may not be setup properly. If the ratio of high to low control signal is between 2 and 4 you can proceed with a Tb TR-FRET.

Use the Kit as a Positive Control in Tb TR-FRET Assays
When running Tb TR-FRET assays, add a small volume of the high and low controls to separate wells, on your assay plate. Place the plate into the instrument and read, according to instructions. A high to low control signal ratio of between 2 and 4 offers you the confidence that the instrument is performing well.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Sequence Detection Systems Spectral Calibration Kit, 384-well (Applied Biosystems™)

This Applied Biosystems® easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™,ROX™, SYBR™ Green, TAMRA™, TET™, and VIC® dyes on the 7900HT Sequence Detection System. The Sequence Detection Systems Spectral 384-Well Calibration kit includes two optical 384-well reaction plates: one preloaded and sealed background plate, and one preloaded and sealed spectral calibration plate with eight separate dye standards.
• 384-well optical reaction plates preloaded with dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, TAMRA™, TET™, and VIC®) are provided for quick and easy calibration of the ABI PRISM® 7900HT Sequence Detection System
• Easy-to-use and provides accurate discrimination of the fluorescence signal of individual dyes in the reaction mixture
• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions Spend Your Time on Research—Not Instrument Calibration This kit's easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 384-well block of the 7900HT Sequence Detection System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

QuantStudio™ 3/5 Spectral Calibration Plate 2 (ABY™, JUN™, MUSTANG PURPLE™ dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for ABY™, JUN™, and MUSTANG PURPLE™ dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. In addition, the formulations of the dyes in this plate improve results with multiplexing by more accurately representing the fluorescent spectra of the respective probes in your real-time PCR experiments. Please refer to the instrument user guide for full instructions on usage.

MUSTANG PURPLE™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for MUSTANG PURPLE® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

DS-31 Matrix Standard Kit (Dye Set D) (Applied Biosystems™)

This kit contains 4 oligonucleotides respectively labeled with 6-FAM™, VIC®, NED™, and ROX™, pre-pooled in a single tube. Suitable for Dye Set D spectral calibration, on ABI PRISM® Analyzers: 3100-Avant™, 3100, and 3730. Supports 4-Dye fragment analysis applications (Linkage Mapping Set v2.5, Mouse Mapping Primers).

The DS-31 Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing labeled DNA fragments on the Applied Biosystems® 3130 and 3100 Series Systems, 3700 DNA Analyzer, and 3730 Series Systems. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary.

For Research Use Only. Not for use in diagnostics procedures.

CountBright™ Absolute Counting Beads, for flow cytometry (Invitrogen™)

CountBright™ absolute counting beads are a calibrated suspension of microspheres that are brightly fluorescent across a wide range of excitation and emission wavelengths (UV to 635 nm excitation and 385-800 nm emission). CountBright™ absolute counting beads are mixed with the cell sample and assayed via flow cytometry. By comparing the ratio of bead events to cell events, absolute numbers of cells in the sample can be calculated. Because CountBright™ beads are mixed in the test sample, absolute cell counts using this single-platform method are more accurate and less complicated than cell concentration determined using multiple-platform testing. CountBright™ absolute counting beads can be used with any sample type, including no-wash/lysed whole blood.

View information about all flow cytometry cell counting beads.

TaqMan™ Low Density Array Spectral Calibration Kit (Applied Biosystems™)

The TaqMan® Low Density Array Spectral Calibration Kit is used to validate the performance of the Applied Biosystems® 7900HT Fast Real-Time PCR Systems with the TaqMan® Low Density Array Block Upgrade. The TaqMan® Low Density Array Spectral Calibration Kit contains four empty TaqMan® Low Density Array, one background tube, and three separate pure dye standards (FAM™, ROX™,VIC®). Insert the loaded calibration arrays during system installation, and the system collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis. To ensure accurate results, we recommend that the performance of your instrument be verified every six months.

QuantStudio™ 3/5 Spectral Calibration Plate 3 (TAMRA™, NED™, Cy™5 dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for TAMRA™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

Pierce™ Intact Protein Standard Mix (Thermo Scientific™)

The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as a quality control standard for LC and LC/MS, LC/MS method development, and intact protein mass measurements.

Verified protein sequences—protein sequences confirmed by MS with the average and monoisotopic masses corresponding to each provided sequence
Recombinant—proteins expressed in E. coli and B. subtilis; no bovine sources
Compatible—protein mixture designed for QC over a wide range of molecular weights (10-70 kDa) and m/z distribution (600-1500 m/z)
Flexible—for use in LC, LC-MS, and direct infusion methods
Stable—provided in a stable lyophilized format

The Pierce Intact Protein Standard Mix contains a mixture of six highly pure recombinant proteins, including human IGF-1 LR3, human thioredoxin, Streptococcus dysgalactiae Protein G, Bovine Carbonic Anhydrase II, Streptococcus Protein AG (chimeric), and Escherichia coli Exo Klenow, expressed in bacteria. These proteins were selected based on their wide range of molecular weights, m/z distribution, and MS fragmentation patterns. After expression and purification, proteins are optimally mixed and lyophilized for stability and convenience. Each vial contains 76 µg of lyophilized protein mixture and does not contain salts or detergents.

Applications:
• Qualitative LC
• LC/MS standardization and method development
• LC/MS intact protein mass measurement and top-down method development
• Digestion protocol optimization

Protein sequences for Pierce Intact Protein Mix components (FASTA format) ›

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7500 Real Time PCR Systems Spectral Calibration Kit II (Applied Biosystems™)

This easy-to-use kit enables you to establish the pure dye spectra and multicomponent values for Cy3®, Cy5®, and Texas Red® dyes on the 7500 Real-Time PCR System. The Applied Biosystems® 7500 Real-Time PCR Systems Spectral Calibration Kit II contains three optical 96-well reaction plates preloaded and sealed with Cy3®, Cy5®, and Texas Red® fluorescent dye standards.

• 96-well optical reaction plates preloaded with Cy3®, Cy5®, and Texas Red® dyes allow quick and easy calibration of the 7500 Real-Time PCR System
• Easy-to-use, preloaded spectral calibration kit allows accurate discrimination of the individual dyes in a reaction mixture when all the dyes are excited simultaneously

Spend Your Time on Research, Not Instrument Calibration
This kit is easy-to-use, preloaded, 96-well optical reaction plates enable users to calibrate the 7500 Real-Time PCR System quickly and easily. Insert the reaction plates during system installation, and the system automatically collects the dye standard's spectral information. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

TetraSpeck™ Microspheres, 0.5 µm, fluorescent blue/green/orange/dark red (Invitrogen™)

The 0.5 µm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.