Shop All Instrument Calibration Kits, Standards & Reagents

Pierce™ Intact Protein Standard Mix (Thermo Scientific™)

The Thermo Scientific Pierce Intact Protein Standard Mix is a high quality mixture of recombinant proteins for use as a quality control standard for LC and LC/MS, LC/MS method development, and intact protein mass measurements.

Verified protein sequences—protein sequences confirmed by MS with the average and monoisotopic masses corresponding to each provided sequence
Recombinant—proteins expressed in E. coli and B. subtilis; no bovine sources
Compatible—protein mixture designed for QC over a wide range of molecular weights (10-70 kDa) and m/z distribution (600-1500 m/z)
Flexible—for use in LC, LC-MS, and direct infusion methods
Stable—provided in a stable lyophilized format

The Pierce Intact Protein Standard Mix contains a mixture of six highly pure recombinant proteins, including human IGF-1 LR3, human thioredoxin, Streptococcus dysgalactiae Protein G, Bovine Carbonic Anhydrase II, Streptococcus Protein AG (chimeric), and Escherichia coli Exo Klenow, expressed in bacteria. These proteins were selected based on their wide range of molecular weights, m/z distribution, and MS fragmentation patterns. After expression and purification, proteins are optimally mixed and lyophilized for stability and convenience. Each vial contains 76 µg of lyophilized protein mixture and does not contain salts or detergents.

Applications:
• Qualitative LC
• LC/MS standardization and method development
• LC/MS intact protein mass measurement and top-down method development
• Digestion protocol optimization

Protein sequences for Pierce Intact Protein Mix components (FASTA format) ›

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3500/3500xL Sequencing Standards, BigDye™ Terminator v1.1 (Applied Biosystems™)

The BigDye® Terminator v1.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v1.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 2.5 µm (Invitrogen™)

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in the range of 645–680 nm.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

Notes:
The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Flow Cytometry Alignment Beads, 2.5 µm, for 633 excitation (A7312).

TaqMan™ RNase P Instrument Verification Plate for 7300/7500 Systems, 96-well (Applied Biosystems™)

The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is used to verify the performance of the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate is a 96-well PCR plate preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye–labeled probe) and template. The 7300 & 7500 Real-Time PCR Systems TaqMan® RNase P Instrument Verification Plate satisfies the same requirements as the Applied Biosystems® 7300 and 7500 Real-Time PCR Systems installations. To pass installation, the instruments must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, Applied Biosystems® recommends that the performance of your instrument be verified every six months.

Alignflow™ Flow Cytometry Alignment Beads for UV Lasers, 2.5 µm (Invitrogen™)

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for UV Lasers are fluorescent-dye infused microspheres that are optimally excitable between 350-370 nm with emission in the range of 400-470 nm, but that can also be excited by a violet laser.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

Notes:
The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Flow Cytometry Alignment Beads, 2.5 µm, for UV excitation (A7304).

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, 384-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 384-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

DS-32 Matrix Standard Set [5FAM™, JOE™, NED™ and ROX™ dyes] for the 310 Genetic Analyzer (Applied Biosystems™)

The Matrix Standard Set is used to generate the "multicomponent matrix" required when analyzing DNA fragments on the 310 Genetic Anlayzer. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the five different colored fluorescent dye-labeled samples in a single capillary.

For Research Use Only. Not for use in diagnostics procedures.

Flow Cytometry Size Calibration Kit (nonfluorescent microspheres) (Invitrogen™)

Our Flow Cytometry Size Calibration Kit has nonfluorescent particle-size calibration standards that provide a simple, accurate way to determine cell sizes by flow cytometry. The kit contains six suspensions of highly uniform polystyrene microspheres with the following diameters: 1.0 µm, 2.0 µm, 4.0 µm, 6.0 µm, 10.0 µm and 15.0 µm. All of the microsphere suspensions are provided in convenient dropper vials.

View additional information for flow cytometry calibration and size reference beads.

QuantStudio™ 3/5 Spectral Calibration Plate 3 (TAMRA™, NED™, Cy™5 dyes), 96-well, 0.2 mL (Applied Biosystems™)

This plate establishes the pure dye spectra and multicomponent values for TAMRA™, NED™, and Cy®5 dyes on Applied Biosystems™ QuantStudio™ 3 and 5 96-well 0.2-mL real-time PCR systems. It enables simultaneous multiple dye calibrations in one plate, significantly reducing effort and time required for dye calibration. Please refer to the instrument user guide for full instructions on usage.

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 6.0 µm (Invitrogen™)

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the range of 515–660 nm.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

Notes:
The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Plus Flow Cytometry Alignment Beads, 6.0 µm, for 488 excitation (A7303).

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, 96-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your 7500, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Ion Library TaqMan™ Quantitation Kit (Ion Torrent™)

The Ion Library TaqMan™ Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library TaqMan Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ System sequencing workflow.

With The Ion Library TaqMan Quantitation Kit You Get:

• Precision— The TaqMan® Assay provides greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods.
• Sensitivity— The protocol supports quantitation of femtomolar amounts of fragment libraries.
• Simplicity— The validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation.
• Versatility— The highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range.
• Efficiency— qPCR-based methods use less material for quantification.

Quantitate Library Input for Use on the PGM™ System
The Ion Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in an Ion fragment library for use during template preparation in the Ion Torrent sequencing workflow. Complete with TaqMan® qPCR mix custom-designed for Ion library adapters and an Ion library control, the kit is a complete solution for determining the optimal amount of starting material needed for high quality sequencing results.

This Kit Includes:

• A custom TaqMan® Assay designed for Ion library adaptors
• An optimized Ion Library Amplification mix
• An adapted E. Coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190bp in size, providing essential components for quality control while maintaining flexibility to accommodate your laboratory needs.

Compatible With a Wide Range of Applications and Instruments
With the Ion Library TaqMan Quantitation Kit, most real-time instruments using either fast or standard cycling programs can be utilized to accurately quantitate genomic and amplicon libraries. These instruments include the Applied Biosystems 7900HT, 7300, 7500, ViiA 7, StepOne™, and StepOnePlus™ Systems. The kit has been validated for quantification of Ion Fragment Libraries. The Ion-specific E. Coli DH10B Library control included in the kit is recommended for use only as a qPCR control and not a sequencing control.

Ion PGM™ Calibration Standard (Ion Torrent™)

The Ion PGM Calibration Standard is a small panel consisting of known sequence content with comprehensive and uniform representation of long homopolymers up to 10-mers. The Ion PGM Calibration Standard can be used with the Ion 314, Ion 316, and Ion 318 workflows for de novo research sequencing applications to allow for greater accuracy of base-calling.

RNaseP Verification Plate, Fast 96-well (Applied Biosystems™)

The Fast 96-Well RNaseP Verification Plate is used to verify your real-time PCR instrument with Fast 96-Well Block. Confirm the performance of your instrument by running the RNaseP assay. Please see your instrument user guide for full instructions on use.

The Fast 96-Well RNaseP Verification Plate is preloaded with the necessary reagents to detect and quantitate genomic copies of the human RNase P gene, a single-copy gene encoding the RNA moiety of the RNase P enzyme. Each well contains preloaded reaction mix (1X TaqMan® Universal PCR Master Mix, RNase P primers, and FAM™ dye-labeled probe) and template.

The verification plate must demonstrate the ability to distinguish between 5,000 and 10,000 genomic equivalents with a 99.7% confidence level for a subsequent sample run in a single well. To ensure accurate results, it is recommended that the performance of your instrument be verified every six months.

Pierce™ Triple Quadrupole Calibration Solution (Thermo Scientific™)

Thermo Scientific Pierce Triple Quadrupole Calibration Solution is a mixture of high purity, ionizable components specifically designed for positive mode calibration of Thermo Scientific Triple Stage Quadrupole instruments.

Features of Triple Quadrupole Calibration Solution:

Strong peaks—mixture of three tyrosine polymers in methanol/formic acid solution
Ready-to-use—load the reference standard into a syringe and inject into the instrument
High-purity—mass spectrometry grade reagents in a non-leachable container
Stable—store at 2 to 8°C for more than one year

Pierce Triple Quadrupole Calibration Solution is a ready-to-use liquid formulation ideal for quickly performing the required routine calibration to maintain the robust performance of Thermo Scientific Mass Spectrometers. The Triple Quadrupole Calibration Solution is manufactured at an ISO 9001 facility and each lot is quality controlled with strict specifications. The stable solution is provided in a leak-proof, high-purity PTFE bottle.

Pierce Triple Quadrupole Calibration Solution can be used to calibrate the TSQ Quantum series, TSQ Discovery series, TSQ Quantum Access Series and TSQ Vantage Series Mass Spectrometer instruments.

More Product Data
Calibration solutions for Thermo Scientific Mass Spectrometers