Shop All Instrument Calibration Kits, Standards & Reagents

Dye Primer/Matrix Standards [5FAM™, JOE™, TAMRA™, and ROX™ dyes] (Applied Biosystems™)

This Dye Primer Matrix Standards are used to generate the multicomponent matrix required when analyzing 5-FAM™, JOE™, TAMRA™ and ROX™-labeled DNA fragments. The standard can be used for Dye Primer cycle Sequencing and Dye Primer Sequenase Sequencing and on the Applied Biosystems® 310, 3100⁄3100-Avant™ and 3130⁄XL Genetic Analyzers. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary.

For Research Use Only. Not for use in diagnostics procedures.

Peptide Retention Standard (Thermo Scientific™)

Thermo Scientific Pierce Peptide Retention Standard is a mixture of five c-terminal amide decapeptides that provide reference elution profiles in reverse-phase HPLC.

Features of Peptide Retention Standard:

• Determine the relative order of peptide elution from a complex mixture
• Efficiently predict peptide elution profiles to simplify identification and save purification time
• Identify peptides of known amino acid composition on reverse phase HPLC columns
• Monitor column performance—efficiency, selectivity and resolution during column aging
• Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities
• Compare reverse-phase columns from different manufacturers

Peptide Retention Standard allows accurate prediction of elution time for peptides of known amino acid composition up to 20 residues in length. The reference standard helps to predict the relative order of peptide elution within a complex mixture, simplifying identification of specified peptides and saving time in peptide purification. By testing the standard frequently throughout the lifetime of a column, it is possible to monitor performance characteristics such as efficiency, selectivity, and resolution during column aging. The standard can also be used to evaluate different reverse phase supports of varying n-alkyl chain lengths and ligand density, as well as columns from different manufacturers.

The Peptide Retention Standard consists of five C-terminal amide decapeptides, denoted S1 to S5, having the generic formula Ac-Arg-Gly-X-X-Gly-Leu-Gly-Leu-Gly-Lys-Amide. Four of these peptides are Na-acetylated with the sequence variation as follows: Gly3-Gly4, Ala3-Gly4, Val3-Gly4 and Val3-Val4. The fifth peptide, Ala3-Gly4, contains a free Na-amino group. This mixture will provide 100-200 injections (approximately 0.1 mg of each peptide) at 0.1 AUFS at 210nm. The Peptide Retention Standard is supplied as a dry film in a sealed vial.

A simple, quantitative method for predicting peptide retention times was developed by Guo, et al. (1985, 1986). Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups.

Retention time is dependent upon the molecular weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule. The accuracy of predicting peptide retention time significantly decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density.

Ion Library TaqMan™ Quantitation Kit (Ion Torrent™)

The Ion Library TaqMan™ Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library TaqMan Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ System sequencing workflow.

With The Ion Library TaqMan Quantitation Kit You Get:

• Precision— The TaqMan® Assay provides greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods.
• Sensitivity— The protocol supports quantitation of femtomolar amounts of fragment libraries.
• Simplicity— The validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation.
• Versatility— The highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range.
• Efficiency— qPCR-based methods use less material for quantification.

Quantitate Library Input for Use on the PGM™ System
The Ion Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in an Ion fragment library for use during template preparation in the Ion Torrent sequencing workflow. Complete with TaqMan® qPCR mix custom-designed for Ion library adapters and an Ion library control, the kit is a complete solution for determining the optimal amount of starting material needed for high quality sequencing results.

This Kit Includes:

• A custom TaqMan® Assay designed for Ion library adaptors
• An optimized Ion Library Amplification mix
• An adapted E. Coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190bp in size, providing essential components for quality control while maintaining flexibility to accommodate your laboratory needs.

Compatible With a Wide Range of Applications and Instruments
With the Ion Library TaqMan Quantitation Kit, most real-time instruments using either fast or standard cycling programs can be utilized to accurately quantitate genomic and amplicon libraries. These instruments include the Applied Biosystems 7900HT, 7300, 7500, ViiA 7, StepOne™, and StepOnePlus™ Systems. The kit has been validated for quantification of Ion Fragment Libraries. The Ion-specific E. Coli DH10B Library control included in the kit is recommended for use only as a qPCR control and not a sequencing control.

ABY™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for ABY® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits) (Invitrogen™)

ArC™ Amine Reactive Compensation Bead Kit is a bead-based compensation tool specifically optimized for use with LIVE/DEAD™ Fixable dead cell stain kits.

• Ready-to-use control
• Eliminates the hassle of heat-treating cells as a control, saving precious sample
• Enables accurate and consistent results

View a selection guide for all fixable viability dyes for flow cytometry.

Ready-to-use control
Unlike other methods that require sample preparation to make compensation controls using cellular material, the ArC Amine Reactive Compensation Bead Kit is ready to use out of the dropper bottle. The kit includes two types of specially modified polystyrene microspheres that have been optimized to react with the amine-reactive dye in the LIVE/DEAD Fixable dead cell stain kits. The ArC reactive beads (Component A) bind any of the amine-reactive dyes and provide a positive signal; the ArC negative beads (Component B) have no reactivity and provide a negative compensation control. This ready-to-use product preserves the original sample material for experiments; precious sample is not used for the creation of compensation controls.

Accurate and consistent results
Unlike biological controls, which can have variable expression levels depending upon disease or development state, the Arc beads provide a bright, consistent signal (see figure below). Consistency in the staining pattern of compensation controls is important for obtaining the right compensation value. If the signal varies with the control material used, the compensation value can be affected, leading to erroneous results.

How it works
One drop of beads is added to a test tube containing LIVE/DEAD Fixable dead cell stain. After incubation with amine-reactive dye, the beads are washed in staining buffer, a drop of negative control beads is added (if required), and the beads are resuspended and analyzed by flow cytometry. The two components provide a distinct positive bead that is at least 50-fold brighter than the negative beads, which can be used to set compensation.

Other compensation products
Besides the ArC Amine Reactive Compensation Beads, AbC™ Anti-Mouse Bead Kit is also available for capture of mouse anti-human antibodies, as well as the AbC™ Anti-Rat/Hamster Bead Kit for capture of rat anti-mouse or hamster anti-mouse antibodies.

Ion S5™ Controls Kit Plus (Ion Torrent™)

The Ion S5™ Controls Kit Plus provides library preparation and templating controls for use with the Ion S5™ and Ion S5™ XL Sequencing Systems. The kit offers greater experimental control and empowers you to assess sample quality, help save time and reagents, and maximize productivity. Each kit contains a CEPH genomic DNA control for up to 3 library preparations, a 200 bp human CEPH control library, and a 400 bp E.Coli control library, for up to 10 template preparations each. The Ion S5 Controls Kit Plus replaces the previous Ion S5™ Controls Kit (Cat. No. A27760).

Note:
This kit is only compatible with the Ion S5 and Ion S5 XL Sequencing Systems.

QuantStudio™ 12K Flex OpenArray™ Calibration Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Calibration Kit is offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray® block type. The calibration kit contains FAM™ dye, as well as a calibration starter kit. The calibration starter kit contains the calibration plaque for background and uniformity, as well as four empty cases for dye loading. Calibration is recommended on a yearly basis. Please see your instrument maintenance guide for full instructions on use.

Alignflow™ Flow Cytometry Alignment Beads for Red Lasers, 6.0 µm (Invitrogen™)

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 6.0 µm Alignflow™ Flow Cytometry Alignment Beads for Red Lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in the range of 645–680 nm.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

Notes:
The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Plus Flow Cytometry Alignment Beads, 6.0 µm, for 633 excitation (A7313).

TaqMan™ RNase P Instrument Verification Plate for ViiA™ 7 System, 384-well (Applied Biosystems™)

Used to perform verification of your ViiA™7 System with 384 Well Block. Please see your instrument maintenance guide for full instructions on use.

JUN™ Dye Spectral Calibration Plate for Multiplex qPCR, Fast 96-well (Applied Biosystems™)

This plate is used for JUN® dye spectral calibration of your 7500 Fast, ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, and QuantStudio™ 12K Flex real-time PCR systems with a Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use. The dye formulations will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

3500/3500xL Sequencing Standards, BigDye™ Terminator v3.1 (Applied Biosystems™)

The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Applied Biosystems® 3500 Series Genetic Analyzer. The DNA contained in the preparation has been lyophilized to maximize stability.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

MeltDoctor™ HRM Calibration Plate, 384-well (Applied Biosystems™)

Applied Biosystem® ready-to-use MeltDoctor™ HRM Calibration Plates contain all the components required for pure dye and HRM calibration, reducing the complexity of getting started with HRM experimentation. These plates provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves.

xMAP™ Classification Calibrator Microspheres (CAL1) (Invitrogen™)

xMAP® Classification (CAL1) Calibrator microspheres, are polystyrene microspheres that are internally labeled with fluorescent dyes. They serve as system Calibrators for Luminex xMAP technology based detectors (such as Luminex 200™) and are intended to normalize the kittings for both classification channels (CL1, CL2), the doublet discriminator channel (DD).
The Luminex® 200™ System is sold separately.

SDS Spectral Calibration Kit (Applied Biosystems™)

This easy-to-use kit establishes the pure dye spectra and multicomponent values for FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™ dyes on the ABI PRISM 7700® Sequence Detection System.

The SDS Spectral Calibration kit includes 8 tubes of pure dye standards for the Applied Biosystems® ABI PRISM 7700® Sequence Detection System.

• Individual tubes containing eight dyes (FAM™, JOE™, NED™, ROX™, SYBR™ Green, VIC™, TAMRA™, and TET™) are provided for quick and easy calibration of the ABI PRISM 7700® Sequence Detection System
• Individual components provide flexibility in dye standards setup



• Includes the Passive Reference I dye standard for signal normalization in all real-time and endpoint reactions

Spend Your Time on Research—Not Instrument Calibration


This kit enables users to calibrate the ABI PRISM 7700® Sequence Detection System quickly and easily. The system automatically collects the dye standard's spectral information as part of the instrument installation procedure. The system's application algorithm then uses the calibration information to ensure accurate data analysis.

Pierce™ HeLa Protein Digest Standard (Thermo Scientific™)

The Thermo Scientific Pierce HeLa Protein Digest Standard is a highly validated mammalian protein digest that may be used as a quality control sample for mass spectrometry (MS) analysis of complex proteomic samples.

Features of HeLa Protein Digest Standard:

Positive control sample—complex mammalian proteome sample protein digest (>15,000 proteins) from the HeLa S3 cell line
Thorough tryptic digestion—prepared using both LysC and trypsin to ensure less than 10% tryptic missed cleavages
Validated peptide quality—less than 10% methionine oxidation and less than 10% lysine carbamylation
Rigorously tested—high-quality, consistent protein digest guaranteed and documented via lot-specific certificates of analysis
Stable—provided in a stable lyophilized format

The protein digest standard is derived from a well-established adenocarcinoma reference HeLa S3 cell line. HeLa S3 cells express over 15,000 proteins with relevant post-translational modifications, making this cell line an ideal standard for complex proteome mass spectrometry applications. The cell lysate has been digested with both LysC and trypsin to minimize tryptic missed cleavages and improve protein sequence coverage. Moreover, unlike other commercially available protein digests for MS, the Pierce HeLa Protein Digest Standard meets stringent quality testing specifications, including peptide quality, digestion efficiency, and lot-to-lot digest uniformity.

Applications:
• Qualitative LC assessment
• LC/MS standardization
• LC/MS method development
• TMT™ Reagent method development

The Pierce HeLa Protein Digest Standard is a lyophilized tryptic peptide mixture that can be used as a quality control standard for liquid chromatography (LC) separation, MS method development and MS performance benchmarking. The digest is specifically formulated for LC/MS experiments and does not contain salts or detergents. Using the digest standard routinely before analysis of complex samples makes it possible to monitor and normalize LC/MS performance between samples and over time.