Shop All Instrument Calibration Kits, Standards & Reagents

ROX™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This ROX™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

SYBR™ Green Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This SYBR® Green dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

TAMRA™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This TAMRA™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

SA MALDI Matrix, Single-Use (Thermo Scientific™)

The Thermo Scientific SA MALDI Matrices are highly purified, recrystallized reagents packaged in convenient single-use tubes sufficient for analyzing up to 96 samples by matrix assisted laser desorption ionization.

Features of SA MALDI Matrices:

• Single-use packaging saves time and reduces waste
• Provides accurate quantity of matrix every time
• Applications: large peptides, whole proteins, lipids

Matrix assisted laser desorption ionization (MALDI) matrices produce the cleanest mass spectrometric (MS) spectra when they are recrystallized and prepared daily, which is inconvenient and typically results in significant waste. The unique packaging of purified, recrystallized sinapinic acid (SA) MALDI matrices makes it easy to prepare high-quality MALDI reagents in minutes.

MALDI and electrospray ionization (ESI) are the most common methods to ionize peptides for MS analysis. MALDI is widely used because it is significantly faster than ESI and the ionized molecules are almost exclusively singly charged, making interpretation easier. Also, MALDI is readily automated and highly sensitive. This powerful method for MS peptide analysis is now easier to perform with our single-use, highly pure MALDI matrices that are exceptionally convenient to use.

Related Products
MALDI Matrix Sample Pack, Single-Use
CHCA MALDI Matrix, Single-Use
DHB MALDI Matrix, Single-Use

Calibration Install Kit, 384-well (Applied Biosystems™)

This kit is used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with 384-well block. It includes calibration plates to perform a spectral calibration with FAM™, VIC®, ROX™, SYBR® Green, TAMRA™, and NED™ dyes, as well as plates to perform region-of-interest (ROI) calibration, normalization calibration, and RNase P verification. Calibration and verification should be done at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

NED™ Dye Spectral Calibration Plate, Fast 96-well (Applied Biosystems™)

This NED™ dye spectral calibration plate can be used to maintain your ViiA™ 7, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, or QuantStudio™ 12K Flex system with Fast 96-well block. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Ion S5™ Controls Kit Plus (Ion Torrent™)

The Ion S5™ Controls Kit Plus provides library preparation and templating controls for use with the Ion S5™ and Ion S5™ XL Sequencing Systems. The kit offers greater experimental control and empowers you to assess sample quality, help save time and reagents, and maximize productivity. Each kit contains a CEPH genomic DNA control for up to 3 library preparations, a 200 bp human CEPH control library, and a 400 bp E.Coli control library, for up to 10 template preparations each. The Ion S5 Controls Kit Plus replaces the previous Ion S5™ Controls Kit (Cat. No. A27760).

Note:
This kit is only compatible with the Ion S5 and Ion S5 XL Sequencing Systems.

Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers, 2.5 µm (Invitrogen™)

Molecular Probes® AlignFlow™ flow cytometry alignment beads are reliable references for aligning, focusing, and calibrating flow cytometers.The 2.5 µm Alignflow™ Flow Cytometry Alignment Beads for Blue Lasers are fluorescent-dye infused microspheres that are excitable at 488 nm with emission in the range of 515–660 nm.

View information for all flow cytometry calibration and size reference beads.

AlignFlow™ beads provide:

• Uniformity—very low FSC CV and intensity CV
• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

The AlignFlow™ beads are highly uniform with respect to size and fluorescence intensity, resulting in low FSC CV and intensity CV. The beads approximate the size, emission wavelength, and intensity of many biological samples and permit the calibration of the flow cytometer’s laser source, optics, and stream flow without wasting valuable and sensitive experimental material.

Bead Labeling
As with all of our flow cytometry standard microspheres, the AlignFlow™ beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of three colors (UV, blue, red) and two sizes (2.5 µm, 6.0 µm) to match your laser.

Notes:
The AlignFlow™ flow cytometry reference beads are provided in updated packaging that includes a Quick Reference Label that puts key information for product use at your fingertips.

These beads replace previous AlignFlow™ flow cytometry reference bead products. This particular product replaces AlignFlow™ Flow Cytometry Alignment Beads, 2.5 µm, for 488 excitation (A7302).

NED™ Matrix Standard (Applied Biosystems™)

The NED™ Matrix Standard can be used to analyze 6-FAM™, VIC®, NED™ and ROX™-labeled DNA fragments in fragment analysis applications. The standard can be used on the Applied Biosystems® 310 Genetic Analyzers. The Data Collection Software for these instruments uses the multicomponent matrix to automatically analyze the four different colored fluorescent dye-labeled samples in a single capillary.

For Research Use Only. Not for use in diagnostics procedures.

Cell Sorting Set-up Beads (for red lasers) (Invitrogen™)

Molecular Probes® Cell Sorting Set-up Beads are reliable standards for flow cytometry sorter instrument setup and calibration. The Cell Sorting Set-up Beads approximate the size, emission wavelength, and intensity of many biological samples, and so can be used to calibrate a flow cytometer’s cell sorting system, including laser source, optics, and stream flow. The beads are optimized for checking cell sorter settings such as drop delay and efficiency (cell loss during sorting).

View information for all flow cytometry calibration and size reference beads.

Cell Sorting Set-up Beads are available for use with UV, blue, green/yellow, and red lasers.These 6.0 µm beads for red lasers are fluorescent-dye infused microspheres that are excitable at 630–640 nm with emission in the range of 645–680 nm.

Cell Sorting Set-Up Beads provide:

• Consistency in production, creating ideal reference standards
• Confidence through reliable, optimal instrument performance
• Compatibility—use with any instrument

Bead Labeling
As with all of our flow cytometry standard microspheres, the Cell Sorting Set-up Beads are stained internally rather than on the surface. The dyes are therefore insulated from environmental interactions that could cause variable fluorescence output, resulting in excellent signal stability. These 6.0 µm fluorescent polystyrene microspheres are supplied as suspensions packaged in dropper vials for convenient dispensation and are available in a choice of four colors (UV, blue, green/yellow, red) to match your laser.

The dyes used in the manufacture of the Cell Sorting Set-up Beads have been carefully selected to provide emission peaks coincident with cells labeled with commonly used fluorescent dyes. The emission profiles for these standards are intentionally narrow in comparison to cells labeled with a corresponding fluorescent dye. Due to their narrow emission profiles, beads of two different fluorescent colors exhibit minimal spectral overlap, and little or no color compensation is needed when setting up for multicolor experiments.

Notes:
These beads are an alternative to PeakFlow™ flow cytometry reference beads.

For optimal verification of the laser alignment of a cell sorter cytometer, please see our selection of Alignflow™ Flow Cytometry Alignment Beads.

Region of Interest (ROI) and Background Plates, Fast 96-well (Applied Biosystems™)

These Region-of-Interest (ROI) and Background Plates are used to maintain the ViiA™ 7, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex real-time PCR systems with Fast 96-well (0.1 mL) blocks. See the instrument maintenance guide for full instructions on use.

AbC™ Anti-Rat/Hamster Bead Kit (Invitrogen™)

The AbC™ Anti-Rat/Hamster Antibody Compensation Bead Kit provides a consistent, accurate, and simple-to-use technique for the setting of flow cytometry compensation when using fluorochrome-conjugated to rat or hamster antibodies with any laser in flow cytometry.

Species reactivity—for use with all isotypes of rat and hamster antibody conjugates
Maximum laser compatibility—for use with all lasers

Accurate compensation is crucial for effective multicolor analysis in flow cytometry. Often, however, cellular-based compensation controls are not completely effective, as many antigens are not highly expressed, and dimly stained cells can lead to inaccurate compensation settings. The AbC™ Anti-Rat/Hamster Bead Kit was developed to address this issue.

View a selection guide for all flow cytometry compensation beads.

How it works
Each kit provides polystyrene microspheres that have fluorescent antibody conjugate capture capacity (positive compensation beads) or are inert (negative compensation beads). After mixing with a fluorophore-conjugated rat or hamster antibody, the two components provide a distinct high-signal positive control with an appropriate negative population that can then be used to set compensation properly regardless of the intensity of the cells in the actual experiment.

Species reactivity
The AbC™ Anti-Rat/Hamster Bead Kit is ideal for compensation with all isotypes of rat (IgG1, IgG2a, IgG2b, IgG2c, IgM) and hamster (Armenian IgG and Syrian IgG) immunoglobulins.

Maximum laser compatibility
All AbC™ Compensation Bead Kits are ideal for use with any excitation source, including the 405 nm laser which has historically provided higher background signals due to auto-fluorescence with other kits on the market.

MeltDoctor™ HRM Calibration Plate, 384-well (Applied Biosystems™)

Applied Biosystem® ready-to-use MeltDoctor™ HRM Calibration Plates contain all the components required for pure dye and HRM calibration, reducing the complexity of getting started with HRM experimentation. These plates provide thermal and optical calibration required for accurate and reproducible discrimination between melt curves.

Calibration Install Kit, Fast 96-well (Applied Biosystems™)

This kit is used to maintain your ViiA 7, QuantStudio 6 Flex, QuantStudio 7 Flex, and QuantStudio 12k Flex real-time PCR systems with Fast 96-well block. It includes calibration plates to perform a spectral calibration with FAM, VIC, ROX, SYBR Green, TAMRA, NED dyes, plates to perform region-of-interest (ROI) calibration, normalization calibration, and to run an RNase P verification. Calibration and verification should be run at least every six months. Please see your instrument maintenance guide for full instructions on use.

The dye formulations for your real-time PCR system will improve results with multiplexing by more accurately representing fluorescent spectra found in your real-time PCR experiments.

Ion Library TaqMan™ Quantitation Kit (Ion Torrent™)

The Ion Library TaqMan™ Quantitation Kit is an easy-to-use, flexible solution for the accurate quantitation of library input for the Ion Torrent semiconductor sequencing workflow. This comprehensive kit provides a TaqMan® qPCR mix and library standard to detect and quantify femtomolar amounts of Ion Fragment Libraries. With universal cycling conditions that allow both fast and standard cycling, the Ion Library TaqMan Quantitation kit supports most real-time platforms. This universal kit provides greater accuracy and specificity compared to non-probe-based methods, delivering simplicity and speed to the PGM™ System sequencing workflow.

With The Ion Library TaqMan Quantitation Kit You Get:

• Precision— The TaqMan® Assay provides greater specificity and accuracy in detecting properly adapted, amplifiable templates over non-probe-based quantitation methods.
• Sensitivity— The protocol supports quantitation of femtomolar amounts of fragment libraries.
• Simplicity— The validated, ready-to-use qPCR standard requires only a simple serial dilution to determine the optimal concentration of library to use for templated bead preparation.
• Versatility— The highly robust qPCR mix accommodates a wide range of cycling conditions and reaction volumes, and combines highly sensitive detection with a broad quantification range.
• Efficiency— qPCR-based methods use less material for quantification.

Quantitate Library Input for Use on the PGM™ System
The Ion Library TaqMan Quantitation Kit facilitates the quantitation of amplifiable template in an Ion fragment library for use during template preparation in the Ion Torrent sequencing workflow. Complete with TaqMan® qPCR mix custom-designed for Ion library adapters and an Ion library control, the kit is a complete solution for determining the optimal amount of starting material needed for high quality sequencing results.

This Kit Includes:

• A custom TaqMan® Assay designed for Ion library adaptors
• An optimized Ion Library Amplification mix
• An adapted E. Coli DH10B library control of a defined size constructed using the Ion Fragment Library Kit to yield an adapted library ~190bp in size, providing essential components for quality control while maintaining flexibility to accommodate your laboratory needs.

Compatible With a Wide Range of Applications and Instruments
With the Ion Library TaqMan Quantitation Kit, most real-time instruments using either fast or standard cycling programs can be utilized to accurately quantitate genomic and amplicon libraries. These instruments include the Applied Biosystems 7900HT, 7300, 7500, ViiA 7, StepOne™, and StepOnePlus™ Systems. The kit has been validated for quantification of Ion Fragment Libraries. The Ion-specific E. Coli DH10B Library control included in the kit is recommended for use only as a qPCR control and not a sequencing control.