Shop All Imaging & Plate Reader Calibration Reagents

FocalCheck™ Microspheres, 6 µm, fluorescent green ring stain/blue throughout (Invitrogen™)

The sharp ring stains exhibited by the 6.0 µm FocalCheck™ fluorescent green ring stained/blue throughout microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy. Correct image registration is indicated when the ring image and disk image of the combination beads are perfectly coincident in all dimensions.

TetraSpeck™ Microspheres, 0.2 µm, fluorescent blue/green/orange/dark red (Invitrogen™)

The 0.2 µm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

FocalCheck™ Thin-Ring Fluorescent Microspheres Kit, 1.0 µm, three suspensions (Invitrogen™)

The FocalCheck Thin-Ring Fluorescent Microspheres Kit is specifically designed to help researchers examine the alignment, sensitivity and stability of confocal laser scanning microscopes. We have treated the microspheres in this kit with dyes to stain the surface of each bead, resulting in a well-defined dye layer that creates a ring in optical sections. These microspheres produce a striking visual representation of instrument misalignment or other optical aberrations, making them ideal tools for optimizing the performance of confocal microscopes. Each suspension in the kit contains beads with a different color configuration. One suspension contains beads with two ring stains (green and red). The second suspension contains beads that have a green ring stain and a dark red stain throughout. The beads in the third suspension have a red ring stain and are stained blue throughout.

Instrument Control Terbium TR-FRET Kit

The Instrument Control Terbium TR-FRET Kit allows for the rapid assessment of instrument setup for terbium (Tb)-based TR-FRET assays. LanthaScreen® and Premo™ assays require specific instrument settings. Proper instrument setup is critical to experimental success. This kit contains one tube each of 1x high and low controls which may also be used as positive control samples when running Tb TR-FRET assays.

The Instrument Control Terbium TR-FRET Kit Allows You to:

• Set up instruments for Tb TR-FRET assays quickly
• Check instrument Tb TR-FRET settings
• Read a high and low Tb TR-FRET signal control on each assay plate

Determine if Your Instrument is Set Up to Read Tb TR-FRET Assays
Setting up your instrument to read Tb TR-FRET is the first step in running a successful Tb TR-FRET assay. This kit allows you to quickly determine if your instrument is set up properly to read Tb TR-FRET assays. Simply add a small volume into separate wells, place the plate into the instrument and read. If the ratio of high to low control signal is below 2 the instrument may not be setup properly. If the ratio of high to low control signal is between 2 and 4 you can proceed with a Tb TR-FRET.

Use the Kit as a Positive Control in Tb TR-FRET Assays
When running Tb TR-FRET assays, add a small volume of the high and low controls to separate wells, on your assay plate. Place the plate into the instrument and read, according to instructions. A high to low control signal ratio of between 2 and 4 offers you the confidence that the instrument is performing well.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

TetraSpeck™ Fluorescent Microspheres Size Kit (mounted on slide) (Invitrogen™)

This TetraSpeck Fluorescent Microspheres Size Kit contains one microscope slide, with six viewing regions. Each of five viewing regions contains microspheres of one size – 0.1, 0.2, 0.5, 1.0 or 4.0 µm. The remaining region contains a mixture of all five bead sizes. Every microsphere is stained with four different fluorescent dyes [365⁄430 nm (blue), 505⁄515 nm (green), 560⁄580 nm (orange), and 660⁄680 nm (dark red)] that have well-separated excitation and emission peaks. Invitrogen’s TetraSpeck™ fluorescent microspheres aid in the calibration of wide field, TIRF and confocal fluorescence microscopes,especially for multicolor applications like co-localization analysis and the derivation of point spread functions critical for deconvolution.

TetraSpeck™ Microspheres, 0.5 µm, fluorescent blue/green/orange/dark red (Invitrogen™)

The 0.5 µm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

FocalCheck™ Microspheres, 15 µm, fluorescent green/orange/dark-red ring stains (Invitrogen™)

The sharp ring stains exhibited by the 15 µm FocalCheck™ fluorescent green/orange/dark-red ring stained microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy. Correct image registration is indicated when the multiple ring images are perfectly coincident in all dimensions.

FocalCheck™ DoubleGreen Fluorescent Microspheres Kit, 6 µm, mounted on slides (Invitrogen™)

The 6.0 µm FocalCheck™ DoubleGreen Fluorescent Microspheres are designed to test spectral separation on the Zeiss META system and other spectral imaging systems. These microspheres are stained with two different fluorescent dyes that appear similar in color by eye but are sufficiently different to be resolved by linear-unmixing techniques. When linear-unmixing data-processing algorithms are applied, the dyes are shown to be spectrally distinct and spatially separated - one appears only with in the outer ring and the other appears throughout the microsphere. The dual-stained microspheres are provided mounted on a microscope slide. The kits also contain two additional slides containing microspheres stained uniformly with each of the individual dyes.

FocalCheck™ Microspheres, 6 µm, fluorescent green/orange/dark-red ring stains (Invitrogen™)

The sharp ring stains exhibited by the 6.0 µm FocalCheck™ fluorescent green/orange/dark-red ring stained microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy. Correct image registration is indicated when the multiple ring images are perfectly coincident in all dimensions.

FP One-Step Reference Kit

Many Fluorescence Polarization (FP) reading instruments require the use of a reference standard for internal calibration of the reader. The FP One Step Reference Kit contains reagents to use as green or red FP references in a single tube or a microplate high throughput screening (HTS) instrument. The green reagents can be used for readings using the following filters: 488 nm ± 5 nm for excitation and 535 nm ± 5 nm for emission (or equivalent). The red reagents can be used for readings using the following filters: 525 nm ± 5 nm for excitation and 590 nm ± 5 nm for emission (or equivalent).

FocalCheck™ Fluorescent Microspheres Kit, 6 µm, mounted on slides (Invitrogen™)

Our popular FocalCheck microspheres have been available in suspension for several years. Now, in response to customer requests, we offer FocalCheck beads pre-mounted on microscope slides. The 6 µm FocalCheck Fluorescent Microsphere Kit features mounted samples of our three best-selling color configurations: fluorescent green/orange/dark red ring stains, fluorescent green ring stain/blue throughout and fluorescent dark red ring stain/green throughout.

TetraSpeck™ Microspheres, 1.0 µm, fluorescent blue/green/orange/dark red (Invitrogen™)

The 1.0 µm TetraSpeck™ microspheres are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

TetraSpeck™ Fluorescent Microspheres Sampler Kit (Invitrogen™)

The TetraSpeck™ Fluorescent Microspheres Sampler Kit contains samples of the 0.1 µm, 0.5 µm, and 4.0 µm TetraSpeck™ beads. TetraSpeck™ beads are stained throughout with four different fluorescent dyes, yielding beads that each display four well-separated excitation/emission peaks - 360/430 nm (blue), 505/515 nm (green), 560/580 nm (orange) and 660/680 nm (dark red). These microspheres can greatly facilitate the calibration of conventional fluorescence microscopes, confocal laser scanning microscopes, and associated image-processing equipment for both scientific and commercial imaging, especially for multicolor applications.

FocalCheck™ Microspheres, 15 µm, fluorescent green ring stain/dark red throughout (Invitrogen™)

The sharp ring stains exhibited by the 15 µm FocalCheck™ fluorescent green ring stained/dark red throughout microspheres produce a striking visual representation of instrument misalignment or other aberrations making them ideal as reference standards for confocal laser-scanning microscopy. Correct image registration is indicated when the ring image and disk image of the combination beads are perfectly coincident in all dimensions.

FocalCheck™ Fluorescence Microscope Test Slide #1, for alignment, intensity, & calibration (Invitrogen™)

The FocalCheck™ fluorescence microscope test slide #1 is specifically designed for calibrating fluorescence microscope systems and evaluating system and filter performance. The slide contains 10 sample areas coated with FocalCheck™ ring stained microspheres ranging in size from 0.5-15 µm that provide a visual representation of instrument misalignment or other aberrations in row A; row B contains a series of microspheres with fluorescence intensities ranging from 0.667-100%.