Shop All Laser Capture Microdissection (LCM) Kits

Arcturus™ Paradise™ PLUS Whole Transcript Reverse Transcription Kit (WT-RT) (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Whole Transcript RT (WT-RT) Reagent System provides an efficient and reliable solution for purifying RNA from archived Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples followed by reverse transcription of whole transcript for use in real-time PCR.

FFPE samples introduce unique challenges for gene expression profiling and gene expression quantitation, including chemical modification and fragmentation of RNA molecules. Storage of FFPE samples adds to these challenges through increased RNA degradation over time.

The Paradise® PLUS WT-RT Reagent System was specifically developed and specially optimized to provide high-quality RNA and robust reverse transcription (RT) from highly fragmented RNA obtained from archived FFPE samples over 20 years old (Figure 2).

Key product features:
• Simple workflow—minimal sample handling reduces chance of contamination (Figure 3)
• Efficient RNA extraction and isolation—saves time and minimizes sample loss
• Optimized RT reactions—generate cDNA representing the entire length of the transcript
• Accurate transcript analysis—results represent high, medium, and low-abundance expressing genes

Optimized RNA Extraction and Isolation from Archived FFPE Tissues
Figure 1 shows a comparison between total RNA yields using the Paradise® PLUS WT-RT Reagent System and a similar commercially available protocol. The average yield of the three replicate isolations illustrates the abundance of total RNA made available using the Paradise® PLUS WT-RT Reagent System. The Paradise® PLUS WT-RT Reagent System produces abundant total RNA for reverse transcription and subsequent qPCR analysis, up to three times more total RNA than obtained when a similar commercially available product was used for the same sample.

Superior Amplification Possible Over Wide Range of Targets
The sensitivity of the Paradise® PLUS WT-RT Reagent System was compared to similar reagent products available on the market that generate cDNA for use in real-time PCR reactions. As Figure 4 illustrates, improved Ct values were seen for all high-, medium- and low-expressing genes when the Paradise® PLUS product was used. Exon-spanning primers at varying distances from the 3’-end of the transcript (500 bp to 5 kb) were used to show increased sensitivity and improved transcription success compared to other commercially available products. The Paradise® PLUS WT-RT Reagent System successfully amplified all genes, suggesting the reagents provided in the kit are optimized to work together, saving you time and effort while producing high-quality results across the entire transcript.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of Arcturus' complete Systems for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS® Systems for Microgenomics permit accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Histogene™ LCM Frozen Section Staining Kit, includes dehydration chemicals, stain, and slides (Applied Biosystems™)

The Arcturus® HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for laser capture microdissection (LCM). Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

Key product features:
• Optimized process—provide superior staining while preserving RNA
• Comprehensive kit—simplify tissue staining and dehydration
• High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity
• Versatile application—obtain high-quality RNA from many tissue types
• Modular design—use with other Arcturus products to produce superior microarray data

Provide Superior Staining while Preserving Nucleic Acids
HistoGene® Staining Solution has been developed by Arcturus® to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene® stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.

Simplify Tissue Staining and Dehydration
The HistoGene® LCM Frozen Section Staining Kit comes complete with all the reagents and supplies needed for preparing frozen tissue sections for LCM.

Retain Low-abundance mRNA
RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene® LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene® samples appears free of degradation.

Maintain RNA Integrity
Tissues prepared for LCM using HistoGene® LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.

Obtain High-quality RNA from Many Tissue Types
Arcturus® has validated the HistoGene® Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene® Kit have yielded quality RNA. Validated tissue types include (Table 1):

Part of the Arcturus® System for Microgenomics
The HistoGene® LCM Frozen Section Staining Kit is part of the Arcturus® Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure® RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. Arcturus® instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, biotin labeling (Applied Biosystems™)

The ARTURUS® Paradise® PLUS Reagent System with Microarray Labeling permits unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Microarray Labeling, Gene Expression Profiling
• Flexibility in Assay Format
• Integrated Systems for Microgenomics

Microarray Labeling, Gene Expression Profiling
The Paradise® PLUS RNA Amplification Kit may be configured to include Turbo Labeling™ for use in microarray experiments. Turbo Labeling Kits offer a proprietary, non-enzymatic technology optimized for the labeling of unmodified, amplified RNA (aRNA) for gene expression profiling. The unmodified aRNA is labeled post-amplification, thereby avoiding the need to incorporate modified nucleotides during RNA amplification. The use of unmodified nucleotides in the amplification process results in unmodified aRNA with higher yields and longer aRNA fragments, thus providing better representation of the mRNA transcript for downstream analysis.

Flexibility in Assay Format
Turbo Labeling Kits include reagents to support labeling of 12 samples using either Cy™3⁄Cy™5 dyes or Biotin for hybridization to cDNA or oligonucleotide arrays.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Histogene™ LCM Immunofluorescence Staining Kit, includes staining components only (Applied Biosystems™)

The ARCTURUS® HistoGene® LCM Immunofluorescence Staining Kit is designed to retrieve high-quality nucleic acids from immunofluorescently-stained, frozen tissue using a quick 15-minute process. The kit provides reagents and slides for convenient and reliable immunoflourescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality.

Key product features are:
• Simplified staining—retrieve high-quality RNA from simplified fluorescent target cell labeling
• Efficient preservation—quick 15-minute process preserves mRNA
• Cold sample protection—chill samples for intact RNA
• Exceptional specificity—benefit from brilliant high-contrast label intensity
• Maximized compatibility—use with many tissue types

Simplified Fluorescent Target Cell Labeling
Identify target cells for Laser Capture Microdissection (LCM) and gene expression analysis using antigen markers to highlight specific surface or intracellular proteins. The HistoGene® LCM Immunofluorescence Staining Kit is specifically designed to retrieve high-quality RNA from immunofluorescently stained frozen tissue. The staining kit provides reagents and slides for convenient and reliable immunoflourescent staining as well as protocols that are streamlined and optimized to maintain nucleic acid quality (Figure 1).

Quick 15-minute Process Preserves RNA
The HistoGene® LCM Immunofluorescence Kit works in less than 15 minutes (only 5 minutes in aqueous environment). The proprietary staining buffer from ARCTURUS® minimizes nucleic acid degradation. Standard immunofluorescence labeling kits typically take over 90 minutes to process samples, compromising the nucleic acid integrity with conditions sub-optimal for single-stranded nucleic acid stability. Electrophoresis and RT-PCR of RNA isolated after using the HistoGene® LCM Immunofluorescence Kits confirm that high-quality RNA is retrieved from microdissected samples (Figures 2 & 3).

Chill Samples for Intact RNA
To increase RNA yield and help ensure its quality, the HistoGene® Cold Block keeps up to four tissue section slides and several tubes containing buffer and antibody solutions chilled while using the HistoGene® LCM Immunofluorescence Kit. The HistoGene® Cold Block is designed for use with the CoolSafe triple density polysterene cooler (Cat. # CSF-Box) and -10°C CoolBrick (Cat. # BRIK-1520) from Diversified Biotech (Figure 4).

Obtain Intact RNA from Many Tissue Types
Our scientists have examined RNA integrity after using the HistoGene® Immunofluorescence Kits on many tissue types with several antibodies. All tissue tested using the HistoGene® Immunofluorescence Kits have yielded high-quality RNA. Tissue⁄antibody combinations validated include:

Get Brilliant High-contrast Label Intensity
The HistoGene® LCM Immunofluorescence Kit employs a biotin-avidin system with Cy3 which leads to exceptionally good staining intensity and specificity. The kit is used with a primary biotinylated monoclonal antibody to an antigen of choice provided by the user. Cy3-conjugated streptavidin is included in the kit (Figure 5).

Part of the ARCTURUS® Systems for Microgenomics
The HistoGene® LCM Immunofluorescence Kit is validated as part of the Arcturus® Complete System for Microgenomics, an integrated solution for utilizing small quantities of RNA for gene expression analysis. Use the HistoGene® LCM Immunofluorescence Kit to immunofluorescently stain your samples. Use the ArcturusXT™ Laser Capture Microdissection instrument to capture pure cell populations of your target cells. The PicoPure® RNA Isolation Kit lets you maximize recovery of RNA from even small numbers of cells. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. ARCTURUS® instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

This kit provides reagents for 2-round in-vitro transcription.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is a validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, Cy3 labeling, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System with Microarray Labeling permits unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Microarray Labeling, Gene Expression Profiling
• Flexibility in Assay Format
• Integrated Systems for Microgenomics

Microarray Labeling, Gene Expression Profiling
The Paradise® PLUS RNA Amplification Kit may be configured to include Turbo Labeling™ for use in microarray experiments. Turbo Labeling™ Kits offer a proprietary, non-enzymatic technology optimized for the labeling of unmodified, amplified RNA (aRNA) for gene expression profiling. The unmodified aRNA is labeled post-amplification, thereby avoiding the need to incorporate modified nucleotides during RNA amplification. The use of unmodified nucleotides in the amplification process results in unmodified aRNA with higher yields and longer aRNA fragments, thus providing better representation of the mRNA transcript for downstream analysis.

Flexibility in Assay Format
Turbo Labeling™ Kits include reagents to support labeling of 12 samples using either Cy™3⁄Cy™5 dyes or Biotin for hybridization to cDNA or oligonucleotide arrays.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, amino allyl, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

This kit provides reagents for 2-round in-vitro transcription, and aminoallyl aRNA labeling.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise PLUS Reagent System is a validated as part of the Arcturus® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. Arcturus Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 1.5 Round Kit, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is a validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 1.5 Round Kit, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise PLUS Reagent System is a validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS qRT-PCR Kit (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System enables unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent system enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries. Achieve unprecedented results in just six steps (Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major gene expression platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories.

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples.

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful gene expression results, the system includes reagents optimized for exceptional recovery of RNA and superior reverse transcription and RNA amplification (Figure 3).

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of Arcturus' complete Systems for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecules otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Histogene™ Refill Kit, includes dehydration chemicals & stain only (Applied Biosystems™)

This kit contains only the dehydration chemicals and stain used with the ARCTURUS® HistoGene® LCM Frozen Section Staining Kit. Designed as a fast-penetrating stain, this kit provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs.

Key product features:
• Optimized process—provide superior staining while preserving RNA
• Comprehensive kit—simplify tissue staining and dehydration
• High-quality RNA yield—retain low-abundance mRNA and maintain RNA integrity
• Versatile application—obtain high-quality RNA from many tissue types
• Modular design—use with other ARCTURUS® products to produce superior microarray data

Provide Superior Staining while Preserving Nucleic Acids
HistoGene® Staining Solution has been developed by ARCTURUS® to stain tissues for LCM subsequently used as sources of nucleic acids. It is a fast-penetrating stain that provides good contrast by differential staining of nuclei (purple) and cytoplasm (light pink). By minimizing the exposure of tissues to water where nucleases may be activated, HistoGene® stain helps to preserve nucleic integrity that may be otherwise compromised when using longer staining protocols.

Retain Low-abundance mRNA
RT-PCR analysis of specific genes from cells captured from tissues processed using the HistoGene® LCM Frozen Section Staining Kit shows retention of both low-abundance mRNA and higher-abundance species. The mRNA profile of HistoGene® samples appears free of degradation.

Maintain RNA Integrity
Tissues prepared for LCM using HistoGene® LCM Frozen Section Staining Kit reagents, supplies and instructions yield high quality RNA.

Obtain High-quality RNA from Many Tissue Types
ARCTURUS® has validated the HistoGene® Kit by examining RNA integrity on many tissue types. All tissues tested to date using the HistoGene® Kit have yielded quality RNA. Validated tissue types include (Table 1):

Part of the Arcturus® System for Microgenomics
The HistoGene® LCM Frozen Section Staining Kit is part of the ARCTURUS® Complete System of Microgenomics and is designed to seamlessly work together to produce high quality expression microarrays. With the PicoPure® RNA Isolation Kit, you can recover a high yield of RNA from as few as 10 cells in a minimal volume. The RiboAmp® RNA Amplification Kit can amplify picograms of your RNA sample into micrograms of amplified RNA (aRNA) that is ready for labeling and hybridization to microarrays. ARCTURUS® instruments and kits increase the reliability and reproducibility of gene expression studies.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, amino allyl, no solvents (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

This kit provides reagents for 2-round in-vitro transcription, and aminoallyl aRNA labeling.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is a validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. Arcturus Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, Cy3 labeling (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System with Microarray Labeling permits unprecedented gene expression analysis of millions of previously inaccessible samples. The Paradise® PLUS Reagent System is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

Key product features:
• Microarray Labeling, Gene Expression Profiling
• Flexibility in Assay Format
• Integrated Systems for Microgenomics

Microarray Labeling, Gene Expression Profiling
The Paradise® PLUS RNA Amplification Kit may be configured to include Turbo Labeling™ for use in microarray experiments. Turbo Labeling™ Kits offer a proprietary, non-enzymatic technology optimized for the labeling of unmodified, amplified RNA (aRNA) for gene expression profiling. The unmodified aRNA is labeled post-amplification, thereby avoiding the need to incorporate modified nucleotides during RNA amplification. The use of unmodified nucleotides in the amplification process results in unmodified aRNA with higher yields and longer aRNA fragments, thus providing better representation of the mRNA transcript for downstream analysis.

Flexibility in Assay Format
Turbo Labeling™ Kits include reagents to support labeling of 12 samples using either Cy™3⁄Cy™5 dyes or Biotin for hybridization to cDNA or oligonucleotide arrays.

Integrated Systems for Microgenomics
The Paradise® PLUS Reagent System is validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis, and features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ARCTURUS®' Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.

Histogene™ Staining Solution (Applied Biosystems™)

This staining solution is for use with the ARCTURURS® HistoGene® LCM Frozen Section Staining Kit.

Designed as a fast-penetrating stain, it provides excellent contrast while preserving nucleic acid integrity and retaining low-abundance mRNAs in frozen tissue sections intended for laser capture microdissection (LCM).

For Research Use Only. Not for use in diagnostics procedures.

Arcturus™ Paradise™ PLUS 2 Round Kit, amino allyl (Applied Biosystems™)

The ARCTURUS® Paradise® PLUS Reagent System permits unprecedented gene expression analysis of millions of previously inaccessible samples. It is an integrated solution for gene expression studies from formalin-fixed, paraffin-embedded (FFPE) tissues, including reagents for tissue staining, RNA isolation and RNA amplification. Amplify RNA from as little as 5 ng of fixed RNA using methods that have been specially optimized to overcome the challenges of cross-linked templates.

This kit provides reagents for 2-round in-vitro transcription, and aminoallyl aRNA labeling.

Key product features:
• Accelerated discovery process – unlock the tissue archives to access previously inaccessible samples
• Simplified procedure – achieve unprecedented results in six steps
• Streamlined application – integrate seamlessly into current workflow
• Efficient workflow – secure downstream success with the Paradise® QC Kit
• Comprehensive system – benefit from the only complete system for formalin-fixed samples

Unlock the Tissue Archives
Currently, tens of millions of tissue samples with known outcomes are available in tissue archives. The Paradise® PLUS Reagent System enables microarray and qRT-PCR analysis of these previously inaccessible samples, allowing retrospective studies to accelerate discoveries.

Achieve Unprecedented Results in Six Steps
Only six steps are required to take you from sample to results (See Figure 1).

Integrate Seamlessly into Current Workflow
The Paradise® PLUS Reagent System is compatible with all major microarray platforms. Robust and reproducible results are achieved using tools and techniques commonly employed in today’s research laboratories (See Figures 2, 3, 4, and 5).

Secure Downstream Success with the Paradise® QC Kit
Not all FFPE samples contain high quality RNA. Identify the best samples for your studies using a simple, real-time PCR research technique, and then proceed with confidence using these pre-qualified samples (See Figure 6).

Benefit from the Only Complete System for Formalin-fixed Samples
The Paradise® PLUS Reagent System is the only available solution that provides all the components necessary to prepare RNA from formalin-fixed, paraffin-embedded tissue samples for gene expression analysis. To ensure successful microarray results, the system includes reagents optimized for exceptional recovery of RNA and superior RNA amplification.

Integrated Systems for Microgenomics
The Paradise PLUS Reagent System is a validated as part of the ARCTURUS® Complete System for Microgenomics®, an integrated solution for utilizing small quantities of RNA for gene expression analysis. This system features the ARCTURUSXT™ Laser Capture Microdissection (LCM) Instrument to procure pure cell populations. ACTURUS® Systems for Microgenomics enable accurate and sensitive microarray assays that reveal molecular signatures otherwise obscured in whole tissue samples or cell mixtures.

For Research Use Only. Not for use in diagnostics procedures.