Shop All MicroRNA Arrays

TaqMan™ Array Human MicroRNA A Cards v2.0 (Applied Biosystems™)

The TaqMan® Array Human MicroRNA A Card v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool A v2.1 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool A v2.1 for situations where sensitivity is of the utmost importance and⁄or sample is limiting.

TaqMan™ OpenArray™ Human MicroRNA Panel, QuantStudio™ 12K Flex (Applied Biosystems™)

The TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex is a fixed-content panel containing validated human TaqMan® MicroRNA Assays derived from Sanger miRBase release v.14. The TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex greatly simplifies workflow, enabling the generation of a comprehensive miRNA profile for up to 48 samples and 43,000 data points in a single working day using minimal hands-on time and no robotics.

• Take the guess-work out of assay performance with functionally validated TaqMan® MicroRNA Assays
• Avoid 2-day workflow interruptions with a streamlined 1-day procedure
• Make quicker decisions and get answers faster with 48 samples/day throughput
• Spend less time validating with microRNA panels that are highly concordant to single-tube assays
• Help save precious samples and reagent costs with nanoliter fluidics

Functionally Validated TaqMan® MicroRNA Assays
Each TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex contains 754 well-characterized human miRNA sequences from the Sanger miRBase v14. All 754 assays have been functionally validated with miRNA artificial templates.

Streamlined Workflow
Only about 45 minutes hands-on time and 2 hrs instrument run time to run 4 panels–the equivalent of 32 × 384-well plates–with no additional robotics required. The workflow is completed in five steps (Fig 1):

1. Split total RNA sample and process in parallel using Megaplex™ Primer Pools A and B and the supporting TaqMan® MicroRNA Reverse Transcription Kit.
2. Resulting cDNA is preamplified prior to real-time PCR analysis using Megaplex™ PreAmp Pools and TaqMan® PreAmp Master Mix.
3. Preamplified cDNA is diluted, mixed 1:1 with TaqMan® OpenArray® Real-Time PCR Master Mix in the 384-well OpenArray® Sample Loading Plate.
4. TaqMan® OpenArray® MicroRNA Panels are automatically loaded using the AccuFill™ System.
5. Up to 4 panels are cycled simultaneously on a QuantStudio™ 12K Flex Real-Time PCR System

High-throughput miRNA Profiling
For high-throughput profiling, the workflow enables up to 4 runs with 4 panels in each run over 8 hours, for a total of 48 samples and over 43,000 data points in a single workday. The TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex provides the advantages of TaqMan® MicroRNA Assays in a high-throughput plate format, and is ideal for medium to large miRNA profiling studies focused on high-throughput biomarker discovery research, MicroRNA regulation of mRNA gene expression, and MicroRNA functional analysis using well-characterized and well-studied miRNAs. Human MicroRNA Panel have also demonstrated a dynamic range of 4 logs or more (Fig 2)

High Concordance to Single-tube Assays
The TaqMan® OpenArray® Human MicroRNA Panel has demonstrated high concordance with an R2 value of 0.95 to the mostly commonly used tool for validation: individual TaqMan MicroRNA Assays (Fig 3). The same panel is used in the TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex, enabling accurate answers to help minimize validation.

Lower Samples Volume and Reduced Reagent Costs
The TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex utilizes nanoliter fluidics, so each reaction uses only 33 nanoliters of sample and reagents, resulting in significant sample volume and reagent cost savings.

Additional Required Reagents
These products are also required to run the TaqMan® OpenArray® Human MicroRNA Panel, QuantStudio™ 12K Flex:

• Megaplex™ Primer Pools (PN 4444750 or 4444766)
• TaqMan® MicroRNA Reverse Transcription Kit (PN 4366596 or 4366597)
• TaqMan® PreAmp Master Mix (PN 4391128)
• TaqMan® OpenArray® Real-Time PCR Master Mix (PN 4462159 or 4462164)
• QuantStudio™ 12K Flex OpenArray® Accessories Kit (PN 4469576)
• OpenArray® 384-well Sample Plates (PN 4406947)
• OpenArray® AccuFill™ System Tips (PN 4457246 or 4458107)

Additionaly, Megaplex™ Primer Pools are required for miRNA reverse transcription and preamplification prior to real-time PCR.

Download OpenArray® Plate Files
OpenArray® Plate Files (TPF) are needed to process your OpenArray® MicroRNA Panel, QuantStudio™ 12K Flex panels on the QuantStudio™ 12K Flex Real-Time PCR System.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Rodent MicroRNA A Array v2.0 (Applied Biosystems™)

The Applied Biosystems® TaqMan® Rodent MicroRNA A Array v2.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 335 and 226 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan® MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details of the included assays is provided in the Array Map file. Purchase of the Megaplex™ RT Primers, Rodent Pool A is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool A for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan® MicroRNA Arrays
provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the the TaqMan® MicroRNA Array v2.0. When sensitivity is of the utmost importance, and/or sample is limiting Megaplex™ PreAmp Primers provide an optional preamplification step. TaqMan® MicroRNA Arrays v2.0 and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with Sanger miRBase v10 to be created in as little as 5 hours, providing the ideal miRNA profiling solution.

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex RT Primers and optional preamplification using Megaplex PreAmp Primers, TaqMan® Universal PCR Master Mix is simply combined with each reaction and pipetted into each of the eight sample loading ports in the TaqMan® Array, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase v10
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® MicroRNA Arrays v2.0. The content is derived from miRBase v10, and provides close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays. Each array (either human or rodent) contains a selection of carefully chosen endogenous control assays, providing you with a variety of options for data normalization. Of particular note is the inclusion of a new mammalian U6 assay, repeated four times on each card, and an assay unrelated to mammalian species, ath-miR159a, providing a process control.

Ideal for miRNA Profiling
The highly streamlined workflow—coupled with the high specificity, sensitivity, and dynamic range of TaqMan® Assays—make this product solution ideal for miRNA profiling, especially when compared to microarrays.

TaqMan™ Array Rodent MicroRNA B Cards v3.0 (Applied Biosystems™)

The TaqMan® Array Rodent MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 306 and 135 unique microRNAs for mouse and rat respectively in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls for each species are included to aid in data normalization and one TaqMan MicroRNA Assay not related to rodent is included as a negative control. Detail on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Rodent Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool B v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan® Array MicroRNA Cards provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and are required to run the TaqMan® Array MicroRNA Card. For greater sensitivity, or when working with limited sample, Megaplex™ PreAmp Primers provide an optional preamplification step prior to real-time analysis. TaqMan® Array MicroRNA Cards and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with miRBase v21 to be created in as little as 5 hours, providing the ideal miRNA profiling solution. Key product features:

Highly specific—quantitate only the biologically active mature miRNAs

Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample
Simplify sample handling and increase sample throughput
Coverage for human, mouse and rat species
An ideal miRNA profiling solution

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex™ RT Primers and optional preamplification using Megaplex™ PreAmp Primers, TaqMan® Universal PCR Master Mix II is simply combined with each reaction and pipetted into each sample loading port in the TaqMan® Array Card, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® Array Cards. The content is derived from miRBase v14 and v15 for human and rodent species respectively, providing close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays.

TaqMan™ OpenArray™ Rodent MicroRNA Panel, QuantStudio™ 12K Flex (Applied Biosystems™)

The TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex is a fixed-content panel containing validated rodent (mouse and rat) TaqMan® MicroRNA Assays derived from Sanger miRBase release v.15. The TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex greatly simplifies workflow, enabling the generation of a comprehensive miRNA profile for up to 48 samples and 43,000 data points in a single working day using minimal hands-on time and no robotics.
• Take the guess-work out of assay performance with functionally validated TaqMan® MicroRNA Assays
• Avoid 2-day workflow interruptions with a streamlined 1-day procedure
• Make quicker decisions and get answers faster with 48 samples/day throughput
• Spend less time validating with microRNA panels that are highly concordant to single-tube assays
• Help save precious samples and reagent costs with nanoliter fluidics

Functionally Validated TaqMan® MicroRNA Assays
Each TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex contains 750 well-characterized Rodent miRNA sequences from the Sanger miRBase v15. All 750 assays have been functionally validated with miRNA artificial templates.

Streamlined Workflow
Only about 45 minutes hands-on time and 2 hrs instrument run time to run 4 panels - the equivalent of 32 × 384-well plates - with no additional robotics required. The workflow is completed in five steps (Fig 1):

1. Split total RNA sample and process in parallel using Megaplex™ Primer Pools A and B and the supporting TaqMan® MicroRNA Reverse Transcription Kit.
2. Resulting cDNA is preamplified prior to real-time PCR analysis using Megaplex™ PreAmp Pools and TaqMan® PreAmp Master Mix.
3. Preamplified cDNA is diluted, mixed 1:1 with TaqMan® OpenArray® Real-Time PCR Master Mix in the 384-well OpenArray® Sample Loading Plate.
4. TaqMan® OpenArray® MicroRNA Panels are automatically loaded using the AccuFill™ System.
5. Up to 4 panels are cycled simultaneously on a QuantStudio™ 12K Flex Real-Time PCR System

High-throughput miRNA Profiling
For high-throughput profiling, the workflow enables up to 4 runs with 4 panels in each run over 8 hours, for a total of 48 samples and over 43,000 data points in a single workday. The TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex provides the advantages of TaqMan® MicroRNA Assays in a high-throughput plate format, and is ideal for medium to large miRNA profiling studies focused on high-throughput biomarker discovery research, MicroRNA regulation of mRNA gene expression, and MicroRNA functional analysis using well-characterized and well-studied miRNAs.

High Concordance to Single-tube Assays
The TaqMan® OpenArray® Rodent MicroRNA Panel has demonstrated high concordance with an R2 value of 0.97 to the mostly commonly used tool for validation: individual TaqMan MicroRNA Assays (Fig 2). The same panel is used in the TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex, enabling accurate answers to help minimize validation.

Lower Samples Volume and Reduced Reagent Costs
The TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex utilizes nanoliter fluidics, so each reaction uses only 33 nanoliters of sample and reagents, resulting in significant sample volume and reagent cost savings.

Additional Required Reagents
These products are also required to run the TaqMan® OpenArray® Rodent MicroRNA Panel, QuantStudio™ 12K Flex:
• Megaplex™ Primer Pools (PN 4444750 or 4444766)
• TaqMan® MicroRNA Reverse Transcription Kit (PN 4366596 or 4366597)
• TaqMan® PreAmp Master Mix (PN 4391128)
• TaqMan® OpenArray® Real-Time PCR Master Mix (PN 4462159 or 4462164)
• QuantStudio™ 12K Flex OpenArray® Accessories Kit (PN 4469576)
• OpenArray® 384-well Sample Plates (PN 4406947)
• OpenArray® AccuFill™ System Tips (PN 4457246 or 4458107)

Additionaly, Megaplex™Primer Pools are required for miRNA reverse transcription and preamplification prior to real-time PCR.

Download OpenArray® Plate Files
OpenArray® Plate Files (TPF) are needed to process your OpenArray® MicroRNA QuantStudio™ 12K Flex Panels on the QuantStudio™ 12K Flex Real-Time PCR System.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 (Applied Biosystems™)

The TaqMan® Array Human MicroRNA Card Set v3.0 is a two card set containing a total of 384 TaqMan® MicroRNA Assays per card. The set enables accurate quantitation of 754 human microRNAs in as little as 5 hours. Included on each array is three TaqMan MicroRNA Assay endogenous controls to aid in data normalization and one TaqMan® MicroRNA Assay not related to human as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool Set v3.0 is required to run this array set. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool Set v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan™ Array Human MicroRNA B Cards v3.0 (Applied Biosystems™)

The TaqMan® Array Human MicroRNA B Card v3.0 contains 384 TaqMan® MicroRNA Assays enabling accurate quantitation of 377 human microRNAs in as little as 5 hours. Three TaqMan MicroRNA Assay endogenous controls are included to aid in data normalization and one TaqMan® MicroRNA Assay not related to human is included as a negative control. Details on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Human Pool B v3.0 is required to run this array. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Human Pool B v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0 (Applied Biosystems™)

The TaqMan® Array Rodent MicroRNA A+B Cards Set v3.0 is a two card set containing a total of 384 TaqMan® MicroRNA Assays per card. The set enables accurate quantitation of 641 and 373 unique microRNAs for mouse and rat respectively in as little as 5 hours. Included on each array is three TaqMan® MicroRNA Assay endogenous controls for each species to aid in data normalization and one TaqMan® MicroRNA Assay not related to rodent as a negative control. Detail on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Rodent Pool Set v3.0 is required to run this array set. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.

TaqMan® Array MicroRNA Cards provide all the advantages of TaqMan® MicroRNA Assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan® MicroRNA Assays in parallel.

Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and are required to run the TaqMan® Array MicroRNA Card. For greater sensitivity, or when working with limited sample, Megaplex™ PreAmp Primers provide an optional preamplification step prior to real-time analysis. TaqMan® Array MicroRNA Cards and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with miRBase v21 to be created in as little as 5 hours, providing the ideal miRNA profiling solution. Key product features:

Highly specific—quantitate only the biologically active mature miRNAs

Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample
Simplify sample handling and increase sample throughput
Coverage for human, mouse and rat species
An ideal miRNA profiling solution

Simplify Sample Handling and Increase Sample Throughput
Following reverse transcription of miRNA targets using Megaplex™ RT Primers and optional preamplification using Megaplex™ PreAmp Primers, TaqMan® Universal PCR Master Mix II is simply combined with each reaction and pipetted into each sample loading port in the TaqMan® Array Card, simplifying sample handling and increasing sample throughput.

Provides Comprehensive Coverage of Sanger miRBase
Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan® Array Cards. The content is derived from miRBase v14 and v15 for human and rodent species respectively, providing close to full coverage of Sanger with the most up-to-date TaqMan® MicroRNA Assays.