Shop All Hepatocytes

Human Plateable Hepatocytes, Transporter Qualified (Gibco™)

Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.

Cells per vial: 4-8 million

Hepatocyte cultures were incubated with radiolabeled taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OCT1 and MDR1, and OATPs and MRP2, respectively. Accumulation rates and biliary excretion index (BEI%) were calculated.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Female (Gibco™)

These plateable cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, Uptake Qualified (Gibco™)

Cryopreserved human hepatocytes for use in transporter-mediated uptake applications.

Cells per vial: 4-8 million

Prototypical hepatic uptake transporter substrates taurocholate and estradiol-17β-glucuronide have been assessed to qualify the transport function of hepatic NTCP (SLC10A1) and OATP1B14/3 (SLCO1B1/3), respectively. The transport of each substrate (radiolabeled) was monitored by scintillation analysis and active transport values (37°C - 4°C) determined by linear regression.

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes

Mouse (CD-1) Cryopreserved Hepatocytes, Male (Gibco™)

These cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, Induction Qualified (Gibco™)

These plateable cryopreserved human hepatocytes are inducible and suitable for use in experiments monitoring in vitro enzyme induction.

Cells per vial: 4-8 million

The potentials of known clinically relevant enzyme inducers represented by 3-methylcholanthrene (3-MC, a prototypical CYP1A enzyme inducer, 3 µM), phenobarbital (PB, a prototypical CYP2B inducer, 1000 µM) and rifampicin (RIF, a prototypical CYP3A enzyme inducer, 10 µM) have been assessed to verify that each lot is inducible.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes

HEP10, Pooled Human Cryopreserved Hepatocytes (Gibco™)

Get the convenience, affordability, and power of a pooled population of hepatocytes in a single vial. HEP10™ Pooled Human Hepatocytes are a representative population of 10 different donor hepatocytes that have been isolated using our novel patent-pending CellStream Isolation Technology™, helping to deliver consistent, predictable results in your drug metabolism studies and other biological assays.

• Pure, highly viable hepatocytes isolated using our CellStream Isolation Technology™
• 10 donor hepatocytes, 5 male and 5 female in each vial
• Verified enzymatic and pathway activity

CellStream™ Isolation Technology
HEP10™ Pooled Human Hepatocytes are made using our novel patent-pending CellStream Isolation Technology™. This process eliminates the disadvantages of density gradient fractionation prior to cryopreservation of the pool. Instead, we gently isolate and purify the pre-screened, high-viability, and metabolically-active hepatocytes from other cells and debris. The result is a remarkably pure pooled hepatocyte preparation with high viability for use in drug metabolism studies and other biological assays.

10 Donors in One Vial
Each vial of HEP10™ Pooled Human Hepatocytes contains hepatocytes from ten individual donors, 5 female and 5 male donors. Custom HEP10 lots can also be prepared from our broad array of high-quality, single-donor cells. Large lots are also available for extensive studies.

Verified Activity
Each pool is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4⁄5, FMO activity, and NTCP, OATP1B3, and OATP transporter pathways.

Ready for Your Drug Metabolism Experiments
HEP10™ Pooled Human Hepatocytes are useful in a wide variety of applications, including intrinsic clearance, transport, toxicology, biochemistry and cell biology studies.,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Mouse (CD-1) Cryopreserved Hepatocytes, Female (Gibco™)

These cryopreserved female mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Plateable Female (Gibco™)

These plateable cryopreserved female rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzymee xpression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, Metabolism Qualified (Gibco™)

These cryopreserved human hepatocytes are suitable for use in plated metabolism applications.

Cells per vial: 4-8 million

Prototypical cytochrome P450 substrates midazolam, tolbutamide, and dextromethorphan were used to assess the enzymatic function of CYP3A4⁄5, CYP2C9 and CYP2D6 respectively. The disappearance of parent was monitored by LC-MS⁄MS analysis and intrinsic clearance (CLint) values determined by linear regression.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Suspension Hepatocytes, Metabolism Qualified, Male, (4-8 x 106 cells) (Gibco™)

These cryopreserved human male hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4-8 million

The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes were analyzed for metabolic activity in each lot. Metabolite formation was measured by standard biochemical assays using LC⁄MS⁄MS or HPLC.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Male (Gibco™)

These cryopreserved male rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, 5-Donor (Gibco™)

These "5-Donor" Human Plateable Hepatocytes are a pooled population of primary hepatocytes produced by combining cells from 5 individual donors in a single vial. Pooling donors helps to minimize lot-to-lot variation and improves predictability of drug metabolism studies and other biological assays.

• Pure, highly viable, plateable hepatocytes
• Lot-specific characterization data
• Thawing and plating process compatible with existing protocols

Consistent cell attachment
These high-quality plateable primary hepatocytes have in vivo-like enzyme expression levels and proper cell morphology, with such in vitro vs. in vivo correlations helping enable more sound decisions regarding the fate of compounds being tested.

5 donors in a single vial
The cells are provided in a cryopreserved format similar to single donor plateable hepatocytes and have been shown to have approximately equal representation of each donor when plated.

Verified activity
Every lot of pooled plateable hepatocytes is tested for Phase I enzyme activity, cell viability, plating efficiency, and morphology.

Human Suspension Hepatocytes, Metabolism Qualified, Female, (4-8 x 106 cells) (Gibco™)

These cryopreserved human female hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4–8 million

The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes were analyzed for metabolic activity in each lot. Metabolite formation was measured by standard biochemical assays using LC/MS/MS or HPLC.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Monkey (cynomolgus) Cryopreserved Hepatocytes, Plateable Male (Gibco™)

These plateable cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Female

These cryopreserved female rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.