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Human Suspension Hepatocytes, Metabolism Qualified, Female, (4-8 x 106 cells) (Gibco™)

These cryopreserved human female hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4–8 million

The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes were analyzed for metabolic activity in each lot. Metabolite formation was measured by standard biochemical assays using LC/MS/MS or HPLC.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

HEP50 Pooled Human Cryopreserved Hepatocytes (Gibco™)

Get the convenience, affordability, and power of a pooled population of hepatocytes in a single vial. HEP50 Pooled Human Cryopreserved Hepatocytes are a representative population of 50 different donor hepatocytes that have been isolated using our novel proprietary CellStream Isolation Technology, helping to deliver consistent, predictable results in your drug metabolism studies and other biological assays.

• Pure, highly viable hepatocytes isolated using our CellStream Isolation Technology
• 50 donor hepatocytes, 25 male and 25 female, in each vial
• Verified enzymatic and pathway activity

CellStream Isolation Technology
HEP50 Pooled Human Hepatocytes are produced using our novel proprietary CellStream Isolation Technology. This process eliminates the disadvantages of density gradient fractionation prior to cryopreservation of the pool. Instead, we gently isolate and purify the pre-screened, high-viability, and metabolically-active hepatocytes from other cells and debris. The result is a remarkably pure pooled hepatocyte preparation with high viability for use in drug metabolism studies and other biological assays.

50 donors in one vial
Each vial of HEP50 Pooled Human Hepatocytes contains hepatocytes from 50 individual donors: 25 female and 25 male.

Verified activity
Each pool is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4/5, and FMO activity, as well as NTCP, OATP1B3, and OATP transporter pathways.

Ready for your drug metabolism experiments
HEP50 Pooled Human Hepatocytes are useful in a wide variety of applications, including intrinsic clearance, transport, toxicology, biochemistry, and cell biology studies.

Note: These products require shipment in liquid nitrogen vapor dewars, so additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Suspension Female

These cryopreserved female rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Monkey (cynomolgus) Cryopreserved Hepatocytes, Plateable Male (Gibco™)

These plateable cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Suspension Hepatocytes, Polymorphic Donors, (9-12 x 106 cells) (Gibco™)

Each lot of cryopreserved human hepatocytes is suitable for use in suspension metabolism applications and has been pharmacogenetically characterized as an homozygous null allele (e.g. CYP2D6*4⁄*4) via genotyping analysis (TaqMan®). The commonly tested Phase I (P450) and Phase II (UGT, SULT) drug metabolizing enzymes are analyzed for metabolic activity in each lot. Metabolite formation is measured by standard biochemical assays using LC-MS/MS or HPLC.

Cells per vial: 9-12 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

•Phase I and II enzyme activities
•Proper morphology
•Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Plateable Hepatocytes, Induction Qualified (Gibco™)

These plateable cryopreserved human hepatocytes are inducible and suitable for use in experiments monitoring in vitro enzyme induction.

Cells per vial: 4-8 million

The potentials of known clinically relevant enzyme inducers represented by 3-methylcholanthrene (3-MC, a prototypical CYP1A enzyme inducer, 3 µM), phenobarbital (PB, a prototypical CYP2B inducer, 1000 µM) and rifampicin (RIF, a prototypical CYP3A enzyme inducer, 10 µM) have been assessed to verify that each lot is inducible.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro/in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes

Rat (Sprague-Dawley) Cryopreserved Hepatocytes, Plateable Female (Gibco™)

These plateable cryopreserved female rat (Sprague-Dawley) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Mouse (CD-1) Cryopreserved Hepatocytes, Plateable Male (Gibco™)

These plateable cryopreserved male mouse (CD-1) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Monkey (cynomolgus) Cryopreserved Hepatocytes, Male (Gibco™)

These cryopreserved male Cynomolgus Monkey hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

HEP10, Pooled Human Cryopreserved Hepatocytes (Gibco™)

Get the convenience, affordability, and power of a pooled population of hepatocytes in a single vial. HEP10™ Pooled Human Hepatocytes are a representative population of 10 different donor hepatocytes that have been isolated using our novel patent-pending CellStream Isolation Technology™, helping to deliver consistent, predictable results in your drug metabolism studies and other biological assays.

• Pure, highly viable hepatocytes isolated using our CellStream Isolation Technology™
• 10 donor hepatocytes, 5 male and 5 female in each vial
• Verified enzymatic and pathway activity

CellStream™ Isolation Technology
HEP10™ Pooled Human Hepatocytes are made using our novel patent-pending CellStream Isolation Technology™. This process eliminates the disadvantages of density gradient fractionation prior to cryopreservation of the pool. Instead, we gently isolate and purify the pre-screened, high-viability, and metabolically-active hepatocytes from other cells and debris. The result is a remarkably pure pooled hepatocyte preparation with high viability for use in drug metabolism studies and other biological assays.

10 Donors in One Vial
Each vial of HEP10™ Pooled Human Hepatocytes contains hepatocytes from ten individual donors, 5 female and 5 male donors. Custom HEP10 lots can also be prepared from our broad array of high-quality, single-donor cells. Large lots are also available for extensive studies.

Verified Activity
Each pool is characterized for CYP1A2, CYP2B6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, CYP3A4, CYP3A4⁄5, FMO activity, and NTCP, OATP1B3, and OATP transporter pathways.

Ready for Your Drug Metabolism Experiments
HEP10™ Pooled Human Hepatocytes are useful in a wide variety of applications, including intrinsic clearance, transport, toxicology, biochemistry and cell biology studies.,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Dog (Beagle) Cryopreserved Hepatocytes, Male (Gibco™)

These cryopreserved male dog (Beagle) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4-8 million

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Rat (Wistar) Cryopreserved Hepatocytes, Plateable Female (Gibco™)

These plateable cryopreserved female rat (Wistar) hepatocytes have been characterized for general Phase I and Phase II drug metabolizing enzyme activities.

Cells per vial: 4–8 million

Get the Results You Need with GIBCO® Hepatocytes
High quality hepatocytes with high viabilities, in vivo-like enzymee xpression levels, and proper cell morphology increase the ability to draw in vitroin vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure that you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:

• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes.

Learn about our full line of hepatocytes.

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) (Gibco™)

Gibco Activated Human Myofibroblastic Hepatic Stellate Cells (MF-HSCs) are produced from isolated pure quiescent human stellate cells (q- HSCs). The cells are obtained from a single donor and provided in a cryopreserved format identical to single-donor plateable human hepatocytes.

Each vial contains a minimum of one million viable cells at passage 5, sufficient for at least four 96-well plates in 2D culture.

Simplified protocol
Activated MF-HSCs are suitable for building hepatic co-culture with primary hepatocytes to study in vitro disease models such as hepatic fibrosis. Cryopreserved cells can be thawed and plated at your convenience in as little as 30 minutes. Try it on your own, or request a demo with an experienced scientist.

Stringent quality control
Each lot is tested for:
• Viability >75%
• Attachment efficiency
• Proper morphology
• Purity based on α-smooth muscle actin (α-SMA)
• Activation in response to Transforming Growth Factor-β1 (TGF-β1)

Human Plateable Hepatocytes, Transporter Qualified (Gibco™)

Plateable cryopreserved human hepatocytes assessed for transporter function using uptake transporter assays at both 4°C and 37°C.

Cells per vial: 4-8 million

Hepatocyte cultures were incubated with radiolabeled taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OCT1 and MDR1, and OATPs and MRP2, respectively. Accumulation rates and biliary excretion index (BEI%) were calculated.

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%
• Attachment efficiency
• Monolayer formation and integrity

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.

Human Suspension Hepatocytes, Transporter Qualified, (4-8 x 106 cells) (Gibco™)

These cryopreserved human hepatocytes are suitable for use in suspension metabolism applications.

Cells per vial: 4-8 million

Taurocholate, digoxin, and estradiol 17β glucuronide (E2-17G), substrates of the uptake and efflux transporters NTCP and BSEP, OATP1B3 and MDR1, OATPs and MRP2, respectively, are included in analyses

Get the Results You Need with GIBCO Hepatocytes
High quality hepatocytes, with high viabilities, in vivo-like enzyme expression levels, and proper cell morphology increase the ability to draw in vitro⁄in vivo correlations and make sound decisions regarding a compound's fate. Experienced technicians and proprietary isolation techniques combined with stringent release specifications ensure you receive the highest quality cryopreserved hepatocytes.

Each lot is tested for:
• Phase I and II enzyme activities
• Proper morphology
• Viabilities generally >75%

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Links:

Learn more about our cryopreserved hepatocytes

Learn about our full line of hepatocytes ,

Note:
These products require shipment in LN2 vapor dewars therefore additional fees may apply.