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C57BL/6 Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), especially mouse ESCs/iPSCs. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos. GSC-6002G and GSC-6202G, these MEF cells are isolated from inbred C57BL/6 mice, mitotically arrested by irradiation, and tested to help ensure performance and minimize the risk of contamination. For our most widely used feeder cells for culturing pluripotent stem cells, see CF1 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs

Help save time with ready-to-use MEFs
Irradiated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of irradiated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.

Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.

C57BL/6 Mouse Embryonic Fibroblasts, MitC-treated (Gibco™)

Mitomycin C (MitC)-treated Gibco C57BL/6 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that support the culture of healthy undifferentiated embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs), especially mouse ESCs/iPSCs. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6002M, these MEF cells are isolated from inbred C57BL/6 mice, mitotically arrested by MitC treatment, and tested to help ensure performance and minimize the risk of contamination. For our most widely used feeder cells for culturing pluripotent stem cells, see CF1 Mouse Embryonic Fibroblasts, Irradiated or CF1 Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Provide optimum support for mouse ESCs and iPSCs

Help save time with ready-to-use MEFs
MitC-treated C57BL/6 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of MitC-treated C57BL/6 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. Culture your ESCs and iPSCs with confidence, knowing that you can rely on these high quality feeder cells.

Provide optimum support for mouse ESCs and iPSCs
While C57BL/6 MEFs can also support human ESCs and iPSCs, these feeder cells work particularly well for culturing mouse ESCs and iPSCs, showing better results compared to CF1 and ICR strain MEFs.

CF6-Neo Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6005G, these MEF cells are isolated from CF1 x C57BL/6 hybrid mice, mitotically arrested by irradiation and tested to help ensure performance and minimize the risk of contamination. For neomycin-resistant MEFs that are inactivated by mitomycin C treatment, see CF6-Neo Mouse Embryonic Fibroblasts, MitC-treated. You may also choose from our broad selection of other MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform research involving drug selection using neomycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated CF6-Neo MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF6-Neo MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to help ensure robust and consistent performance in your research. These MEFs have successfully been used with ESC/iPSC lines from other species but should be tested for compatibility if used for such an application.

Perform research involving drug selection using neomycin-resistant cells
CF6-Neo MEFs express neoR from a pPGKneobPa transgene, making them highly resistant to neomycin and geneticin (G418). Each lot is tested for tolerance to various G418 concentrations, and the threshold range for survival is reported in the CoA to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing G418 selection.

CF6-Neo Mouse Embryonic Fibroblasts, MitC-treated (Gibco™)

Mitomycin C (MitC)-treated Gibco CF6-Neo Mouse Embryonic Fibroblasts (MEFs) are neomycin-resistant feeder cells that support healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6005M, these MEF cells are isolated from CF1 x C57BL/6 hybrid mice, mitotically arrested by MitC-treatment, and tested to help ensure performance and minimize the risk of contamination. For neomycin-resistant MEFs that are inactivated by irradiation, see CF6-Neo Mouse Embryonic Fibroblasts, irradiated. You may also choose from our broad selection of other MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform research involving drug selection using neomycin-resistant cells

Help save time with ready-to-use MEFs
MitC-treated CF6-Neo MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF6-Neo MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to help ensure robust and consistent performance in your research. These MEFs have successfully been used with ESC/iPSC lines from other species but should be tested for compatibility if used for such an application.

Perform research involving drug selection using neomycin-resistant cells
CF6-Neo MEFs express neoR from a pPGKneobPa transgene, making them highly resistant to neomycin and geneticin (G418). Each lot is tested for tolerance to various G418 concentrations, and the threshold range for survival is reported in the CoA to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing G418 selection.

CF1 Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco CF1 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that are ideal for supporting healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos.GSC-6001G and GSC-6201G, these widely-used MEF cells are isolated from outbred CF1 mice, mitotically arrested by irradiation, and tested to ensure safety and performance. CF1 MEFs are traditional feeder cells for culturing pluripotent stem cells; for MEFs derived from ICR mice, see Mouse (ICR) Inactivated Embryonic Fibroblasts.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Rely on a widely-used product

Help save time with ready-to-use MEFs
Irradiated CF1 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF1 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have been used successfully with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Rely on a widely-used product
These CF1 MEFs are used in many labs worldwide and have been cited in various publications. They have been successfully used to support undifferentiated pluripotent stem cells at densities ranging from 2 x 104–5.3 x 104 cells/cm2.

B6-Puro Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco B6-Puro Mouse Embryonic Fibroblasts (MEFs) are puromycin-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6220G, these MEF cells are isolated from C57BL/6J-Tg(pPwL88puro)2EMS/J mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or DR4 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with puromycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated B6-Puro MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of B6-Puro MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with puromycin-resistant cells
B6-Puro MEFs express puromycin N-acetyltransferase (pac) from a pPWL88puro transgene, making them highly resistant to puromycin. Each lot is tested for tolerance to various puromycin concentrations, and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing puromycin selection.

Human Dermal Fibroblasts, neonatal (HDFn), Proliferating Culture, Prepared in Medium 106 and LSGS (Gibco™)

Secondary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.

Mouse (ICR) Inactivated Embryonic Fibroblasts (Gibco™)

Mouse embryonic fibroblasts (MEFs) are used as a feeder layer when culturing mouse and human embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). They are isolated from mouse (strain ICR) embryos. The cells are expanded and inactivated by γ irradiation and cryopreserved. Prior to cryopreservation, the cells are tested for viability (>80% post-thaw viability), verified for growth arrest by plating inactivated cells at low density and monitoring for foci of cell growth after 14 days in culture, and also tested for their ability to support growth of mouse embryonic stem cells (mESCs), human embryonic stem cells (hESCs), and marker expression (Oct 4).

Human Dermal Fibroblasts, adult (HDFa) (Gibco™)

Primary human dermal fibroblasts isolated from adult skin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 12 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

CF1 Mouse Embryonic Fibroblasts, MitC-treated (Gibco™)

Mitomycin C (MitC)-treated Gibco CF1 Mouse Embryonic Fibroblasts (MEFs) are feeder cells that are ideal for supporting healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs) in culture. Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. Nos. GSC-6001M and GSC-6201M, these widely-used MEF cells are isolated from outbred CF1 mice, mitotically arrested by MitC-treatment, and tested to ensure safety and performance. For MEFs that are mitotically arrested by irradiation, see CF1 Mouse Embryonic Fibroblasts, Irradiated.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Rely on a widely-used product

Help save time with ready-to-use MEFs
Irradiated CF1 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, and expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of CF1 MEFs is tested for bacteria, fungi, mycoplasma, and mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Rely on a widely-used product
These CF1 MEFs are used in many labs worldwide and have been cited in various publications. They have been successfully used to support undifferentiated pluripotent stem cells at densities ranging from 2 x 104–5.3 x 104 cells/cm2.

DR4 Mouse Embryonic Fibroblasts, irradiated (Gibco™)

Irradiated Gibco DR4 Mouse Embryonic Fibroblasts (MEFs) are multidrug-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6204G, these MEF cells are isolated from DR4 x CF1 hybrid mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or CF6-Neo Mouse Embryonic Fibroblasts, MitC-Treated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with MEFs that are resistant to neomycin/geneticin, puromycin, hygromycin, and 6-thioguanine

Help save time with ready-to-use MEFs
Irradiated DR4 MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of DR4 MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ESCs to help ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with multidrug-resistant cells
DR4 MEFs express transgenes that make them resistant to four different drugs: geneticin (G418), puromycin, hygromycin, and 6-thioguanine. Each lot is tested for tolerance to various concentrations of these drugs and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing drug selection.

Human Dermal Fibroblasts, neonatal (HDFn) (Gibco™)

Primary human dermal fibroblasts isolated from neonatal foreskin, cryopreserved at the end of the primary culture.
• Viability at least 70%
• ≥500,000 viable cells/vial
• Capable of at least 16 population doublings
• Mycoplasma: Not Detected
• Hepatitis B: Not Detected
• Hepatitis C: Not Detected
• HIV-1: Not Detected
• Bacteria, yeast, and other fungi: Not Detected

Human Dermal Fibroblasts, adult (HDFa), Proliferating Culture, Prepared in Medium 106 and LSGS (Gibco™)

Secondary cultures established from cryopreserved cells, grown to approximately 50% confluence in T-25 flasks.