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Medium 200 Gibco™

Medium 200 and phenol red-free Medium 200 (Medium 200PRF) are sterile, liquid tissue culture media intended for use as one component in a complete culture environment for normal human large vessel endothelial cells. When supplemented with LSGS or LSGS Kit, these media will support the plating and proliferation of endothelial cells at culture densities between 2.5 x 103 cells/cm2 and 1 x 105 cells/cm2. Additional applications may include primary isolation of endothelial cells.

Both Medium 200 and Medium 200PRF are basal culture media containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. These media do not contain antibiotics, antimycotics, hormones, growth factors, or proteins. These media are HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and proliferation of human large vessel endothelial cells, these media must be supplemented with Low Serum Growth Supplement (LSGS) or Low Serum Growth Supplement Kit.

Medium 200PRF Gibco™

A sterile, liquid medium for the culture of human large vessel endothelial cells. Medium 200PRF is Medium 200 prepared without phenol red. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.