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Shop All Serum-Free & Specialty Media

CD BHK-21 Production Medium (Gibco™)

Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

CD BHK-21 Production Medium is manufactured as follows:
WithWithout
• L-glutamine• Phenol Red
• Dry powder formatProtein hydrolysates

Gibco® CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. The dry powder format is easy to reconstitute, since it has no hydrolysates in the formulation. Adapting cells from serum-containing media is a rapid and simple procedure. BHK-21 cells can achieve high cell densities and maintain high cell viability in this medium.

Gibco® CD BHK-21 Production Medium is suitable for large-scale suspension culture. Because it does not require supplementation with serum or other undefined components, Gibco® CD BHK-21 Production Medium eliminates the risks associated with serum, while allowing you to achieve consistent virus titers and simplifying downstream purification.

cGMP Manufacturing
Gibco® CD BHK-21 Production Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

FreeStyle™ CHO Expression Medium (Gibco™)

FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation.

This medium requires supplementation with 8 mM glutamine before use. Gibco® FreeStyle™ CHO Expression Medium features:

• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco® FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.

Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco® FreeStyle™ CHO cells in suspension can be cultured directly into Gibco® FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO® FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ CHO Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Keratinocyte-SFM (1X) (Gibco™)

Contains L-glutamine. Keratinocyte-SFM is supplied with prequalified human recombinant Epidermal Growth Factor 1-53 (EGF 1-53) and Bovine Pituitary Extract (BPE) in separate packaging. Components are not sold separately.

Note:
A Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

StemPro™ Chondrogenesis Differentiation Kit (Gibco™)

The StemPro® Chondrogenesis Differentiation Kit has been developed for the chondrogenic differentiation of mesenchymal stem cells (MSCs) in tissue-culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the chondrogenesis pathway and generate chondrocytes. Using StemPro® Chondrogenesis Differentiation Kit in combination with StemPro® MSC SFM or MesenPRO RS™ Medium provides a standardized culture workflow solution for MSC isolation, expansion, and differentiation into collagen matrix-producing chondrocytes.

OptiPRO™ SFM (Gibco™)

OptiPRO™ SFM is a serum-free, animal origin-free culture medium designed for growth of several kidney-derived cell lines including MDBK, MDCK, PK-15 and VERO for virus or recombinant protein production. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7. Gibco® OptiPRO™ SFM features:
• Serum-free, animal origin-free, low protein formulation
• Direct adaptation for many popular cell lines

Serum-free, animal origin-free, low protein formulation
Gibco® OptiPRO™ SFM is formulated with a very low protein concentration (< 10 µg/ml), allowing for easier purification of your recombinant protein.

Direct adaptation for many popular cell lines
Gibco® OptiPRO™ SFM has been designed for growth of kidney-derived cell lines such as MDCK, MDBK, VERO and PK-15. In addition, OptiPRO™ SFM has successfully grown other cell lines, such as Hela, BHK-21 and COS-7, allowing you to choose a single medium for multiple cell lines.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
For supply chain continuity, we manufacture Gibco® OptiPRO™ SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

CD BHK-21 Production Medium (Gibco™)

Gibco® CD BHK-21 Production Medium is a chemically defined, protein-free cell culture medium. It is designed to support the growth of BHK-21 cells in serum-free suspension culture. This medium is suitable for large-scale manufacturing applications.

CD BHK-21 Production Medium is manufactured as follows:
WithWithout
• L-glutamine• Phenol Red
• Dry powder formatProtein hydrolysates

Gibco® CD BHK-21 Production Medium is the first cell culture medium supporting complete serum-free and protein-free processes for BHK-21 cells. The dry powder format is easy to reconstitute, since it has no hydrolysates in the formulation. Adapting cells from serum-containing media is a rapid and simple procedure. BHK-21 cells can achieve high cell densities and maintain high cell viability in this medium.

Gibco® CD BHK-21 Production Medium is suitable for large-scale suspension culture. Because it does not require supplementation with serum or other undefined components, Gibco® CD BHK-21 Production Medium eliminates the risks associated with serum, while allowing you to achieve consistent virus titers and simplifying downstream purification.

cGMP Manufacturing
Gibco® CD BHK-21 Production Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

LHC Basal Medium (1X) (Gibco™)

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation.

LHC Basal Medium (1X), liquid

Applications:
Formulated for the growth of bronchial epithelial cells with further supplements.

Recommended storage conditions:
2° to 8°C.

Shelf life:
12 months

Intended use(s)
Research use only



LHC-8 Medium and LHC-9 Medium


Applications:


Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.


Performance and quality testing:

Performance tested in a growth assay using BEAS-2B cells.

Recommended storage conditions:
-5°C to -20°C.

Intended use(s)
Research use only

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.

Sf-900™ II SFM, no methionine, no cystine (Gibco™)

Sf-900™ II SFM (without methionine and cystine) is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Gibco® Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 × 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Gibco® Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Gibco® Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco® Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

EpiLife™ Medium, with 60 µM calcium (Gibco™)

A sterile, liquid medium prepared with 60 µM calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of an appropriate growth supplement prior to use. EpiLife® greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife® medium is HEPES and bicarbonate buffered, and is designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.

Neurobasal™-A Medium (Gibco™)

Neurobasal®-A Medium is a basal medium formulated to meet the special cell culture requirements of post-natal and adult brain neuronal cells when used with GIBCO® B-27® Supplement. Neurobasal®-A Medium can be used to grow neuronal cells from hippocampus, cortex and other regions of the brain. Neurobasal®-A Medium allows for both long and short term maintenance of homogeneous populations of neuronal cells without the need of an astrocyte feeder layer.

Add Your Own Supplements
All Neurobasal® media contain no L-glutamine, L-glutamic acid, or aspartic acid. Neurobasal® media must be supplemented with 0.5 mM L-glutamine or GlutaMAX™-I Supplement and either a serum-free supplement (such as B-27® Supplement or N-2 Supplement), or with serum. For initial plating, you should add 25 µM glutamic acid.

Quality Control Testing
All Neurobasal® media are tested for pH, osmolality, endotoxin, and the absence of bacterial and fungal contamination. The osmolality of Neurobasal®-A was demonstrated to be optimal for postnatal and adult CNS neurons.

Neurobasal®-A Medium contains Phenol Red. If Phenol Red will interfere with your applications, we also provide Neurobasal®-A Medium Minus Phenol Red.

Neurobasal®-A Medium is optimized for post-natal and adult brain neurons. For growing pre-natal and embryonic neurons, we provide Neurobasal® Medium.

See the entire Neurobasal® product line.

Learn more about our neural cell culture products.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Keratinocyte AOF Growth Kit (Gibco™)

Growth kit for the animal origin-free culture of primary human keratinocytes. Contains 1 each of EpiLife Basal Medium (M-EPI-500-CA), Supplement S7 (S-017-5), and Coating Matrix Kit (R-011-K).

StemPro™ Osteogenesis Differentiation Kit (Gibco™)

For Complete Differentiation of Human Mesenchymal Stem Cells into Bone Cells

StemPro® Osteogenesis Differentiation Kit has been developed for the osteogenic differentiation of mesenchymal stem cells (MSCs) in tissue culture vessels. The kit contains all reagents required for inducing MSCs to be committed to the osteogenesis pathway and generate
immature and mature osteocytes. Using StemPro® Osteogenesis Differentiation Kit in combination with MesenPRO RS™ Medium or StemPro® MSC SFM provides a standardized culture workflow solution for MSC isolation, expansion and differentiation into mineralized matrix-producing osteocytes.

Floor Plate Specification Supplement (Gibco™)

Floor Plate Cell Specification Supplement is a component of the Gibco™ PSC Dopaminergic Neuron Differentiation Kit, available here for separate purchase. It enables the specification of PSCs to floor plate cells.

CD Hybridoma AGT™ Medium (Gibco™)

Gibco® CD Hybridoma Medium is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:
• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin-free, protein-free and chemically defined formulation
• Convenient, easy-to-use Advanced Granulation Technology (AGT™) format

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin-free, protein-free and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Convenient, easy-to-use Advanced Granulation Technology (AGT™) format
Our Advanced Granulation Technology™ (AGT™) format is an easy-to-use dry powder format. The granules dissolve instantly and pH-adjusts for fast media preparation. Complete reconstitution instructions are available in the product manual.

Product Use
For research use/further cell culture manufacturing. Not for use in diagnostic procedures. Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® CD Hybridoma Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

EpiLife™ CF Kit (Gibco™)

A sterile, liquid medium prepared without calcium chloride for the long-term, serum-free culture of human epidermal keratinocytes and human corneal epithelial cells. This basal medium requires the addition of calcium plus an appropriate supplement prior to use. Calcium chloride is provided as a separate component with each bottle of medium. EpiLife greatly extends the in vitro lifespan of human epidermal keratinocytes compared to other serum-free media systems. This basal media is animal origin free and contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. EpiLife® medium is HEPES and bicarbonate buffered, and is designed for use in an incubator with an atmosphere of 5% CO2 and 95% air.