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Sf-900™ II SFM Gibco™

Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Gibco™ Prostate Cancer Cell Culture Starter Kit, North America Gibco™

Gibco Cancer Cell Culture Starter kits provide researchers with a convenient package of reagents for the culture and transfection of cancer cell types frequently used in cancer research. Cancer Cell Culture Starter kits are currently available for breast, lung, prostate, and liver cancer cells.

Each starter kit includes the following products for cancer cell culture and transfection:
• Basal media
• Fetal bovine serum
• Penicillin-streptomycin
• TrypLE Express Enzyme
• Lipofectamine 3000 Transfection Reagent
• Opti-MEM I
• Nunc Cell Culture Treated EasYFlasks

The Cancer Cell Culture Starter kits contain the most commonly used reagents to help you start your cancer cell culture quickly and achieve successful transfections, based on a review of hundreds of cancer research publications in which the following cell lines were used:
• Prostate cancer: DU145, PC-3, LNCaP
• Lung cancer: A549, NCI-H975, PC-14, NCI-H1299
• Breast cancer: MCF-7, MDA-MB-231, T-47D, BT-474
• Liver cancer: Hep G2, Hep 3B

The Cancer Culture Starter kits also include a suggested protocol for the culture, dissociation (cell passage), and transfection of the most-published cancer cell lines. Achieve peace of mind and quickly initiate your experiments with these starter kits, the components of which may also be purchased separately.

Medium 200 Gibco™

Medium 200 and phenol red-free Medium 200 (Medium 200PRF) are sterile, liquid tissue culture media intended for use as one component in a complete culture environment for normal human large vessel endothelial cells. When supplemented with LSGS or LSGS Kit, these media will support the plating and proliferation of endothelial cells at culture densities between 2.5 x 103 cells/cm2 and 1 x 105 cells/cm2. Additional applications may include primary isolation of endothelial cells.

Both Medium 200 and Medium 200PRF are basal culture media containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. These media do not contain antibiotics, antimycotics, hormones, growth factors, or proteins. These media are HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and proliferation of human large vessel endothelial cells, these media must be supplemented with Low Serum Growth Supplement (LSGS) or Low Serum Growth Supplement Kit.

CD 293 Medium (1X) Gibco™

CD 293 Medium is a chemically-defined, protein-free medium optimized for the growth of suspension cultures of 293 cells. CD 293 Medium contains no proteins or peptides of animal, plant, or synthetic origin. There are also no undefined lysates or hydrolysates in the formulation.

Formulated without L-glutamine for increased stability; add 20 ml of 200 mM L-glutamine or 20 ml of GlutaMAX™-I Supplement per 1,000 ml of medium prior to use.

A 293 Medium Master file has been submitted to the FDA. If you wish to cross-reference a Master file for CD 293 Medium, please contact Technical Services or your local Sales Representative.

Essential 6™ Medium Gibco™

Essential 6 Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and the spontaneous or directed differentiation of human pluripotent stem cells (PSCs). In addition, Essential 6 Medium can be used as a base for custom media for the culture of PSCs. The formulation is based on a medium originally developed by Guokai Chen et. al. (1) in the laboratory of James Thomson and published as 'E6.' With only six essential components, Essential 6 Medium helps minimize variability. To complete your workflow with a matching reduced-variability PSC culture medium developed by the same lab, use Essential 8 Medium.

Essential 6 Medium enables:
Differentiation—does not contain bFGF, which inhibits differentiation
Reprogramming—does not contain TGFβ, which has a negative effect on reprogramming efficiency
Flexibility—provides a flexible format where the levels of TGFβ and bFGF can be adjusted and additional components can be added to match a given application

Differentiation
Unlike other media used in PSC culture, Essential 6 Medium does not contain bFGF or TGFβ. As such, Essential 6 Medium can support the formation of embryoid bodies. It has also been used as a base for the directed differentiation of various cell types in the endodermal, mesodermal, and ectodermal lineages (2), including motor neurons (3).

Reprogramming
Essential 6 Medium allows for defined and feeder-free reprogramming when used with bFGF. The formulation supports somatic cell reprogramming using a variety of methods, including episomal vectors (4) and CytoTune (Sendai virus), and is optimized to help ensure maximum cell health and pluripotency with minimal variability.

Flexibility
Essential 6 Medium is xeno-free and contains only the essential components needed for stem cell culture, minus bFGF and TGFβ. This provides a basal medium that will maximize cell health and pluripotency while allowing levels of TGFβ and bFGF to be adjusted and additional components to be added to match a given application.

Commercialized in partnership with Cellular Dynamics International.

References:
(1) Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011 8(5):424-9.
(2) Lippmann ES, Estevez-Silva MC, Ashton RS. Defined human pluripotent stem cell culture enables highly efficient neuroepithelium derivation without small molecule inhibitors. Stem Cells. 2014 32(4):1032-42.
(3) Lippmann ES, Williams CE, Ruhl DA, Estevez-Silva MC, Chapman ER, Coon JJ, Ashton RS. Deterministic HOX patterning in human pluripotent stem cell-derived neuroectoderm. Stem Cell Reports. 2015 4(4):632-44.
(4) Yu J, Hu K, Smuga-Otto K, Tian S, Stewart R, Slukvin II, Thomson JA. Human induced pluripotent stem cells free of vector and transgene sequences. Science. 2009 324(5928):797-801.

Grace's Insect Medium (2X), supplemented Gibco™

Grace's Supplemented Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). This 2x formulation is intended for use in the agarose overlay when performing the plaque assay. We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

With
• Lactalbumin hydrolysate
• Yeastolate
• L-glutamine
• Sodium bicarbonate

The complete formulation is available. Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Supplemented Insect Medium, 2x is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Supplemented Insect Medium, 2x contains lactalbumin hydrolysate and yeastolate, and is commonly diluted with sterile water, Fetal bovine Serum and agarose before use in the agarose overlay, as described in the product manual.

Grace's Insect Medium, unsupplemented Gibco™

Grace's Insect Medium was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. One supplemented, Grace's Unsupplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21. Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). Gibco® Grace's Unsupplemented Insect Medium can be used during transfection of insect cells, or for protein labeling studies (with methionine). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This Grace's Insect Medium is modified as follows:

WithWithout
• L-glutamine • Lactalbumin hydrolysate
• Sodium bicarbonate • Yeastolate
• Methionine

The complete formulation is available. Grace's Unsupplemented Insect Medium does not contain lactalbumin hydrolysate or yeastolate, commonly found in the supplemented version. Gibco® Grace's Unsupplemented Insect Medium is also made without methionine for protein labeling studies.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

cGMP Manufacturing and Quality System
Gibco® Grace's Unsupplemented Insect Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Grace's Unsupplemented Insect Medium requires supplementation with 10% Fetal Bovine Serum (FBS), 50 mg/L methionine, 3.33 g/L lactalbumin hydrolysate and 3.33 g/L yeastolate before use as a growth medium. Grace's Unsupplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.

Grace's Insect Medium, supplemented Gibco™

Grace's Insect Medium, Supplemented, was originally designed for the growth of cells from the Australian Emperor Gum Moth, Antherea eucalypti. Grace's Supplemented Insect Medium is now widely used for the growth of Spodeptera frugiperda cells, Sf9 and Sf21.

Sf9 and Sf21 cells are routinely used to express recombinant protein using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® media options for a range of insect cell culture applications.

Formulation
This Grace's Insect Medium contains lactalbumin hydrolysate, yeastolate, L-glutamine, and sodium bicarbonate. The complete formulation is available. Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate not commonly found in the unsupplemented version.

Dual site cGMP manufacturing and quality system
Gibco® Grace's Supplemented Insect Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical product made in our Scotland facility (11605-045). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Grace's Supplemented Insect Medium contains lactalbumin hydrolysate and yeastolate, and is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. Grace's Supplemented Insect Medium requires ambient CO2 in order to maintain physiological pH.

Medium 200PRF Gibco™

A sterile, liquid medium for the culture of human large vessel endothelial cells. Medium 200PRF is Medium 200 prepared without phenol red. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.

FreeStyle™ F17 Expression Medium Gibco™

FreeStyle™ F17 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4–8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco® FreeStyle™ F17 Expression Medium features:

• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin–free, protein-free, and chemically defined formulation
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F, and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6–7 × 106 viable cells per mL, and supports CHO-S cells at a peak density of 8–9 × 106 viable cells per mL. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75–150 µg/mL IgG in 293-F cells.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD CHO AGT™ Medium Gibco™

CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.

When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Essential 8™ Medium Gibco™

Essential 8™ Medium is a xeno-free and feeder-free medium specially formulated for the growth and expansion of human pluripotent stem cells (PSCs). Originally developed by Guokai Chen et al. in the laboratory of James Thomson (published as 'E8') and validated by Cellular Dynamics International, Essential 8™ Medium has been extensively tested and proved to maintain pluripotency in multiple iPSC lines. In addition, Essential 8™ Medium has been used to scale up production of iPSCs and shown to support iPSC growth for >50 passages without any signs of karyotypic abnormalities, along with maintaining the ability of iPSCs to differentiate into all three germ line lineages.

Features of Essential 8™ Medium include:

• Consistent—reduced variability compared to existing feeder-free culture media
• Robust—reliable and robust cultures with a xeno-free, cGMP, 8-component medium
• Cost effective—economical and scalable PSC culture compared to other feeder-free media

Reduced Variability
Essential 8™ Medium is xeno-free and contains only the 8 essential components needed for stem cell culture. Unlike other media that contain over 20 highly variable ingredients, Essential 8™ Medium is produced under cGMP and has an optimized formulation and growth factor levels to help ensure maximum cell health, pluripotency, and growth, with minimal variability.

Reliable and Robust Cultures
Essential 8™ Medium has been show to support pluripotent stem cell growth and provide cultures with superior morphology and growth kinetics compared to other feeder systems.

Cost Effective
Essential 8™ Medium is provided in a convenient two-component kit (500 ml basal medium & 10 ml supplement), and when used with vitronectin (VTN-N), provides a cost effective, defined system for feeder-free culture of human pluripotent stem cells (PSCs).

Essential 8™ Medium is commercialized in partnership with Cellular Dynamics International.

PSC Definitive Endoderm Induction Kit Gibco™

Gibco™ PSC Definitive Endoderm Induction Kit consists of two xeno-free media that enable efficient induction of human pluripotent stem cells (PSCs) to definitive endoderm. Unlike other methods that require multiple components and take five or more days, the PSC Definitive Endoderm Induction Kit can generate ≥90% CXCR4+/PDGFRα- definitive endoderm cells with only two components in just two days. Each medium is supplied as a 1X complete medium, requiring no mixing in of additional components, and the resultant definitive endoderm is capable of differentiating into midgut/hindgut, pancreatic endoderm, or liver bud progenitors that express relevant physiological markers.

Generation of high quality definitive endoderm
Definitive endoderm generated using the PSC Definitive Endoderm Induction Kit is capable of differentiating into downstream lineages such as midgut/hindgut (expressing CDX2), pancreatic endoderm (expressing PDX2), and liver bud progenitors (expressing AFP).

Rapid production of definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing definitive endoderm in only two days.

Generation of highly pure definitive endoderm
The PSC Definitive Endoderm Induction Kit is capable of producing highly pure populations of definitive endoderm from pluripotent stem cells, showing ≥90% CXCR4+/PDGFRα- across multiple lines and high expression of key markers SOX17 and FOXA2.

No mixing in of components required
Each medium is supplied at a 1X concentration, requiring no mixing in of reagents. Simply thaw the media and add to PSC culture.

FreeStyle™ 293 Expression Medium Gibco™

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features:

• Complete and ready-to-use format containing GlutaMAX™ supplement
• Ability to support suspension growth and transfection of 293-F cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use with the FreeStyle™ 293 Expression systems
• Scalability in spinner flasks and bioreactors

Complete and ready-to-use format containing GlutaMAX™ supplement
Gibco® FreeStyle™ 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. Gibco® FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.

Ability to support suspension growth and transfection of 293-F cells
Gibco® 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco® FreeStyle™ 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent and Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein production
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). The FreeStyle™ 293 Expression System combines Gibco® FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. The FreeStyle™ MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. The FreeStyle™ MAX Expression System combines Gibco® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ 293 Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

Universal bag packaging
Gibco® universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

Product use
Customers using Gibco® FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

B-27™ Plus Neuronal Culture System Gibco™

The B-27 Plus Neuronal Culture System is a next-generation media system that provides the highest rate of in vitro survival of primary rodent and human stem cell-derived neurons. Comprised of B-27 Plus Supplement (50X) and Neurobasal Plus Medium, this system is an evolution of the most cited neuronal cell culture products, B-27 Supplement and Neurobasal Medium. It features an optimized formulation, upgraded manufacturing process, and more stringent quality control of raw materials and final product. These improvements enable the highest neuronal cell survival, improved electrophysiological activity, and enhanced functional maturity versus other neuronal cell culture media systems.

The B-27 Plus Neuronal Culture System offers:
• At least 50% higher survival rate of primary rat neurons compared to classic B-27 Supplement and Neurobasal Medium
Highest survival rate of primary rat, mouse, and human stem cell-derived neurons compared to other commercially available serum-free neuronal media systems
Improved electrophysiological activity and maturation—more consistent, stable, and synchronized spontaneous activity in primary rodent neurons after 10 days compared to BrainPhys™ Neuronal Medium
Accelerates neurite outgrowth and increases the number of synaptic-positive puncta

Available as a 50X, 10 mL supplement with 500 mL basal medium, the B-27 Plus Neuronal Culture System can seamlessly replace other neuronal cell culture systems, such as classic B-27 Supplement and Neurobasal Medium. The individual components of the system are also available for purchase separately.

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