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Shop All Serum-Free & Specialty Media

Medium 106 (Gibco™)

A sterile, liquid medium for the culture of human dermal fibroblasts. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.

AIM V™ Medium (research grade), AlbuMAX™ Supplement (Gibco™)

Contains AlbuMAX® supplement (bovine serum albumin) substituted for human serum albumin. Contains L-glutamine, streptomycin sulfate at 50 µg/ml, and gentamicin sulfate at 10 µg/ml.

MarrowMAX™ Bone Marrow Medium (Gibco™)

Gibco® MarrowMAX™ Bone Marrow Medium is a fully-supplemented medium developed for the short term culture of bone marrow and other hematopoietic cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® MarrowMAX™ Bone Marrow Medium features:

• Superior performance
• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Superior performance
Gibco® MarrowMAX™ Bone Marrow Medium outperforms other commercially available media containing Giant Cell Tumor (GCT) conditioned media, providing consistent performance with a higher mitotic index and superior chromosomal morphology (learn more about the superior performance of MarrowMAX™ medium here).

Ready-to-use format
Gibco® MarrowMAX™ Bone Marrow Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 60 days.

Quality and performance testing
Every lot of Gibco® MarrowMAX™ Bone Marrow Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Bone marrow cells from the aspirate of a normal adult donor are cultured for 24 hours in Gibco® MarrowMAX™ Bone Marrow Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, mycoplasma and endotoxin.

Unique, optimized formulation
Gibco® MarrowMAX™ Bone Marrow Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), gentamicin, and L-glutamine. It also contains a novel human stromal cell conditioned medium that consists of a unique blend of hematopoietic growth factors. Gibco® MarrowMAX™ Bone Marrow Medium does not contain GCT conditioned medium.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® MarrowMAX™ Bone Marrow Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

IPL-41 Insect Medium (1X) (Gibco™)

Gibco® IPL-41 Insect Medium was originally designed for the growth of Spodeptera frugiperda (Sf9) cells and the expression of recombinant Autographa Californica Nuclear Polyhedrosis Virus (rAcNPV) using the Baculovirus Expression Vector System (BEVS). We offer a variety of Gibco® Media options for a range of insect cell culture applications.

This IPL-41 Insect Medium is modified as follows:

WithWithout
• L-glutamine • Tryptose phosphate broth
• Sodium bicarbonate • Yeastolate

The complete formulation is available. IPL-41 Insect Medium contains unique components required for insect cell culture, including malic acid, fumaric acid, and alpha-ketoglutaric acid.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.

Dual Site cGMP Manufacturing and Quality System
Gibco® IPL-41 Insect Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer a comparable Gibco® IPL-41 Insect Medium product made in our Scotland facility (11405-057). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Gibco® IPL-41 Insect Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS) before use. IPL-41 Insect Medium requires ambient CO2in order to maintain physiological pH.

LHC-8 Medium (1X) (Gibco™)

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation.

LHC Basal Medium (1X), liquid

Applications:
Formulated for the growth of bronchial epithelial cells with further supplements.

Recommended storage conditions:
2° to 8°C.

Shelf life:
12 months

Intended use(s)
Research use only



LHC-8 Medium and LHC-9 Medium


Applications:


Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.


Performance and quality testing:

Performance tested in a growth assay using BEAS-2B cells.

Recommended storage conditions:
-5°C to -20°C.

Intended use(s)
Research use only

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.

Sf-900™ II SFM (Gibco™)

Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

CTS™ AIM-V™ Medium, without phenol red, without antibiotics (Gibco™)

CTS AIM-V Medium is the first commercially available serum-free formulation for proliferation and/or manipulation of T cells, dendritic cells, and other primary or immortalized cells. CTS AIM-V Medium is an FDA-cleared class-II medical device that is intended for human ex-vivo tissue and cell culture processing applications and is manufactured in compliance with cGMP. It contains L-glutamine and is formulated without phenol red and without antibiotics. CTS AIM-V Medium can be supplemented with human AB serum or CTS Immune Cell SR and various cytokines or growth factors depending on the application.

• cGMP manufactured in compliance with 21 CFR part 820
• Supports expansion of human peripheral blood T-cells including CAR T cells
• Supports expansion of activated human dendritic cells and other immune cells
• Cleared by FDA for human ex-vivo tissue and cell culture processing applications
• Proven clinical use as an ancillary reagent in Provenge™ (sipuleucel-T), the first therapeutic cancer vaccine approved by the FDA [1]
• Adherence to supplier-related responsibilities of USP <1043>*

Security of supply
CTS AIM-V Medium is a defined serum-free formulation that contains no bovine-derived components at the primary component level. The product can be scaled to meet future clinical and commercial demand. Alternate manufacturing sites are available as further risk mitigation.

CTS quality
Gibco CTS products are manufactured at a site that uses methods and controls that conform to cGMP for medical devices, 21 CFR Part 820. Our FDA-registered manufacturing sites are ISO 13485- and ISO 9001–certified. Gibco CTS products follow USP <1043> 'ancillary materials for cell, gene, and tissue-engineered products' within the responsibilities applicable to a supplier.*

Accompanied by documentation to support your regulatory filing
Specific intended-use statements, full documentation traceability, and convenient access to our Drug Master File (DMF) are available.

Immune cell applications
CTS AIM-V Medium is cited in many peer-reviewed journals and used in multiple immune cell clinical applications such as activated dendritic cells. CTS AIM-V Medium supports expansion of T cells, T cell subsets, and virus-specific T cells. CTS AIM-V Medium can also be used to support natural killer cells combined with feeders. Each lot of CTS AIM-V Medium is pre-qualified using Jurkat cells, an immortalized human T lymphocyte line, to help ensure consistent performance.

What you get
CTS AIM-V Medium is available with L-glutamine, but phenol red, gentamicin sulfate, and streptomycin sulfate have been removed to meet global regulatory requirements for ancillary materials used in cell therapy manufacturing. For convenience, CTS AIM-V Medium is available in a 1000-mL bottle for small volume users as well as a 10-L bag for larger volume users interested in closed systems.

*Other aspects of USP <1043> will be the responsibility of the end-user to assess. Thermo Fisher Scientific cannot fulfill USP <1043> in regards to application and therapy-specific aspects (e.g., residual assessment and removal of the AM).

1. Madan RA, Gulley JL. Sipuleucel-T: harbinger of a new age of therapeutics for prostate cancer. Expert Rev Vaccines. 2011 10(2):141-50.

Medium 171 (Gibco™)

A sterile, liquid medium for the culture of human mammary epithelial cells. This basal medium requires the addition of MEGS (S-015-5) prior to use.

CD OptiCHO™ AGT™ Medium (Gibco™)

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

LHC-8 Medium (1X), without gentamicin (Gibco™)

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. This is a supplemented medium. For maximum flexibility and convenience, we also offer LHC basal medium.

Applications:
Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.

Performance and quality testing:
Performance tested in a growth assay using BEAS-2B cells.

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.

AmnioMAX™-II Complete Medium (Gibco™)

Gibco® AmnioMAX™ - II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ - II Complete Medium features:

• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Ready-to-use format
Gibco® AmnioMAX™ - II Complete Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to ten days.

Quality and performance testing
Every lot of Gibco® AmnioMAX™ - II Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ - II Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation
Gibco® AmnioMAX™ - II Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ - II Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD OptiCHO™ AGT™ Medium (Gibco™)

CD OptiCHO™ medium is specifically designed to offer high performance and yield with recombinant CHO cells in a chemically defined fed-batch environment. The protein-free, animal origin free formulation is designed to provide consistency and reduces the need to screen for adventitious agents. CD OptiCHO™ medium builds on Gibco® CD CHO’s legacy of consistent cell growth and titers, while offering up to 40% better titer and cell growth over select competitors. Thanks to its low osmolality and advanced CHO CD EfficientFeed™ Liquid Nutrient Supplement Kit feeding options, CD OptiCHO™ medium is capable of gaining 2-5 fold increases in fed-batch productivity over batch culture.

When to Use CD OptiCHO™ Medium
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD CHO medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Cryopreserved Hepatocyte Plating Medium (CHPM) (Gibco™)

Cryopreserved Hepatocyte Plating Medium (CHPM) used for plating Gibco® cryopreserved human hepatocytes for optimal attachment and monolayer formation.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our Hepatic Cell Culture reagents

Hybridoma-SFM, powder (Gibco™)

Gibco® Hybridoma SFM is a serum-free, very low-protein medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® Hybridoma SFM features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, very low-protein formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® Hybridoma SFM provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Once reconstituted, Gibco® Hybridoma SFM, powder is a complete medium that is ready-to-use. No supplementation is required.

Serum-free, very low-protein formulation
Gibco® Hybridoma SFM is a serum-free, very low-protein formulation, allowing for easier purification of your protein of interest. Gibco® Hybridoma SFM contains < 20 µg/ml total protein.

Product Use
For Research Use Only or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® Hybridoma SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® Hybridoma SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD Hybridoma Medium (Gibco™)

CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® CD Hybridoma Medium features:

• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin–free, protein-free, and chemically defined formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco® CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin–free, protein-free, and chemically defined formulation
Gibco® CD Hybridoma Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Product use
Customers using Gibco® CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® CD Hybridoma Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.