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Dynamis™ Medium (Gibco™)

Gibco® Dynamis™ liquid medium is specifically designed to offer the highest batch and fed-batch culture performance and yield with recombinant CHO cells in a chemically defined environment. The chemically defined, protein-free, animal origin component-free formulation provides the power to achieve high titers, start process development faster and streamline or simplify transfer to manufacturing scale.

Power to achieve higher titers
Dynamis™ AGT™ Medium is fortified to help maximize recombinant CHO cell growth and protein expression in suspension batch culture, offering 170% greater production than select competitive media with simple glucose supplementation by day 14. Cultures grown in Dynamis™ AGT™ Medium extend to even higher titers demonstrating good cell viability out to day 21. This medium can also provide up to 60% greater fed-batch growth and production over glucose-only fed-batch culture for selected cell lines and applications when used with EfficientFeed™ C+ AGT™ Supplement.

Power to start process development faster
As a fortified medium, Dynamis™ AGT™ provides the power to get to a high cell density over 10 million cells per mL faster than competitive products and sustains that cell density longer by just maintaining glucose levels. The viable cell growth achieved make it a good choice for scale-up if you have started with CD FortiCHO™ medium. When paired with EfficientFeed™ C+ AGT™ Supplement, it provides a fast and efficient way to develop a process.

The power to streamline transfer to manufacturing
Dynamis™ liquid medium is made by reconstitution of Dynamis™ AGT™ dry format into the liquid format. Therefore, you simplify scale-up and manufacturing in AGT™ dry format because you know the liquid will behave exactly like the AGT™ format. The AGT™ dry format scale-up provides easy to reconstitute (just add water) use including auto-pH and auto-osmolarity. This can help provide efficiencies in supply chain complexity, reconstitution complexity and therefore overall expense.

When to Use Dynamis™ Medium
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

When to Consider Another Gibco® Medium

Consider CD OptiCHO™ Medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD CHO Medium when:
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

Consider CD DG44 Medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

Gibco® is a registered trademark of ThermoFisher Scientific Inc. Dynamis™ and CD FortiCHO™ are trademarks of Thermo Fisher Scientific Inc., ActiCHO(TM) is a trademark of GE Healthcare Biosciences AB, BalanCD(R) is a registered trademark of Irvine Scientific Sales Co., Cellvento(R) is a registered trademark of Merck KGAA, EXCELL(R) is a registered trademark of SAFC, HyCell(R) is a registered trademark of GE Healthcare Biosciences AB, and PowerCHO(TM) is a registered trademark of Lonza.

VP-SFM AGT™ Medium (Gibco™)

VP-SFM AGT™ is a serum free, ultra low protein medium (5 µg/ml) in a new easy-to-use granular format. VP-SFM AGT™ is designed for the growth of VERO cells for virus production. It is also suitable for the growth of COS-7, MDCK, BHK-21 and Hep2 cells and for recombinant protein production.

FreeStyle™ 293 Expression Medium (Gibco™)

FreeStyle™ 293 Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transfection of 293-F cells in suspension without adaptation. The medium is complete and ready-to-use, with no supplementation required. Gibco® FreeStyle™ 293 Expression Medium features:

• Complete and ready-to-use format containing GlutaMAX™ supplement
• Ability to support suspension growth and transfection of 293-F cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use with the FreeStyle™ 293 Expression systems
• Scalability in spinner flasks and bioreactors

Complete and ready-to-use format containing GlutaMAX™ supplement
Gibco® FreeStyle™ 293 Expression Medium is ready to use. It does not require the addition of serum, glutamine, or surfactants. Gibco® FreeStyle™ 293 Expression Medium contains GlutaMAX™ supplement to minimize toxic ammonia build-up and improve cell viability and growth in an easy-to-use format.

Ability to support suspension growth and transfection of 293-F cells
Gibco® 293-F, 293-H, and FreeStyle™ 293-F cells in suspension can be cultured directly into Gibco® FreeStyle™ 293 Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ 293 Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ 293 Expression Medium can be transfected using lipid based transfection reagents, such as 293fectin™ reagent and Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ 293 Expression Medium is animal origin–free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ 293 Expression System and FreeStyle™ MAX Expression System for protein production
The FreeStyle™ 293 Expression System is a complete, suspension cell culture system for generating large amounts of mammalian recombinant protein (see figure). The FreeStyle™ 293 Expression System combines Gibco® FreeStyle™ 293 Expression Medium with 293fectin™ reagent, a cationic-lipid formulation designed to transfect suspension FreeStyle™ 293-F cells at high efficiency. The FreeStyle™ MAX Expression System is a breakthrough technology for rapid and high-yield mammalian protein production. The FreeStyle™ MAX Expression System combines Gibco® FreeStyle™ Media, FreeStyle™ MAX Reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ 293 Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ 293 Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

Universal bag packaging
Gibco® universal bags offer flexible packaging that allows you to integrate a single-use, disposable large volume media container with a wide variety of automated cell culture instruments, disposable bioreactor systems, and sterile connection processes, minimizing the need for costly customization.

Product use
Customers using Gibco® FreeStyle™ 293 Expression Medium in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco® FreeStyle™ 293 Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Essential 8™ Adaptation Kit (Gibco™)

The Essential 8™ Adaptation Kit includes Essential 8™ Medium and recombinant human Laminin 521 (rhLaminin-521) for optimal survival during transition from feeder-dependent to feeder-free culture. rhLaminin-521 has been proven to promote cellular survival under stressful conditions in the absence of small molecule inhibitors. In addition, rhLaminin-521 has been shown to support PSC growth for >20 passages without any signs of karyotypic abnormalities and to maintain the ability of PSCs to differentiate into all three germ line lineages. rhLaminin-521 can also be purchased separately.

The Essential 8 Adaptation Kit is:
• Robust–transition cells between feeder-dependent and feeder-free culture systems with minimal effort
• Physiologically relevant–expand cells with minimal differentiation
• Scalable–passage stem cell lines as single cells with no impact to genetic stability

Trouble-free transition
When used with Essential 8 Medium, rhLaminin-521 provides a robust culture matrix that supports pluripotent stem cell adherence and expansion during the transition from feeder-dependent culture systems.

Confident expansion
rhLaminin-521 is expressed in blastocysts and therefore provides an environment mimicking the human stem cell niche.

Easy scale-up
rhLaminin-521 promotes stable pluripotent stem cell maintenance as single cells in the absence of ROCK inhibitors.

FreeStyle™ CHO Expression Medium (Gibco™)

FreeStyle™ CHO Expression Medium is an animal origin–free, chemically defined, protein-free medium specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation.

This medium requires supplementation with 8 mM glutamine before use. Gibco® FreeStyle™ CHO Expression Medium features:

• Formulation without hypoxanthine and thymidine for use with or without dhfr systems
• Ability to support high-density suspension growth and transient transfection of CHO-S cells
• Animal origin–free, protein-free and chemically defined formulation
• Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
• Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems
Gibco® FreeStyle™ CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems. Non-dhfr systems require the addition of HT Supplement before use.

Ability to support high-density suspension growth and transient transfection of CHO-S cells
Gibco® FreeStyle™ CHO cells in suspension can be cultured directly into Gibco® FreeStyle™ CHO Expression Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ CHO Expression Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations. Cells cultured in Gibco® FreeStyle™ CHO Expression Medium can be transfected using lipid-based transfection reagents, such as Freestyle™ MAX reagent.

Animal origin–free, protein-free and chemically defined formulation
Gibco® FreeStyle™ CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Easy use as part of the FreeStyle™ MAX CHO Expression System for protein production
The FreeStyle™ MAX CHO Expression System is a breakthrough technology for rapid (see figure) and high-yield mammalian protein production in 30 mL and 1 L batch sizes (see figure). The FreeStyle™ MAX CHO Expression System combines GIBCO® FreeStyle™ media, FreeStyle™ MAX reagent, and either FreeStyle™ CHO-S cells or FreeStyle™ 293-F cells in one kit.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ CHO Expression Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ CHO Expression Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

cGMP manufacturing and quality system
Gibco® FreeStyle™ CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Keratinocyte-SFM (1X), without calcium chloride (Gibco™)

Contains L-glutamine but no calcium chloride. Keratinocyte-SFM is supplied with prequalified human recombinant Epidermal Growth Factor 1-53 (EGF 1-53) and Bovine Pituitary Extract (BPE) in separate packaging. Components are not sold separately.

Hybridoma-SFM, powder (Gibco™)

Gibco® Hybridoma SFM is a serum-free, very low-protein medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco® Hybridoma SFM features:
• Ability to support suspension and stationary hybridomas and myelomas
• Complete, ready-to-use formulation
• Serum-free, very low-protein formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco® Hybridoma SFM provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250x Cholesterol Lipid Concentrate.

Complete, ready-to-use formulation
Once reconstituted, Gibco® Hybridoma SFM, powder is a complete medium that is ready-to-use. No supplementation is required.

Serum-free, very low-protein formulation
Gibco® Hybridoma SFM is a serum-free, very low-protein formulation, allowing for easier purification of your protein of interest. Gibco® Hybridoma SFM contains < 20 µg/ml total protein.

Product Use
For Research Use Only or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® Hybridoma SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP Manufacturing and Quality System
Gibco® Hybridoma SFM is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

MarrowMAX™ Bone Marrow Medium (Gibco™)

Gibco® MarrowMAX™ Bone Marrow Medium is a fully-supplemented medium developed for the short term culture of bone marrow and other hematopoietic cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® MarrowMAX™ Bone Marrow Medium features:

• Superior performance
• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Superior performance
Gibco® MarrowMAX™ Bone Marrow Medium outperforms other commercially available media containing Giant Cell Tumor (GCT) conditioned media, providing consistent performance with a higher mitotic index and superior chromosomal morphology (learn more about the superior performance of MarrowMAX™ medium here).

Ready-to-use format
Gibco® MarrowMAX™ Bone Marrow Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to 60 days.

Quality and performance testing
Every lot of Gibco® MarrowMAX™ Bone Marrow Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Bone marrow cells from the aspirate of a normal adult donor are cultured for 24 hours in Gibco® MarrowMAX™ Bone Marrow Medium before measuring the mitotic index and chromosome banding resolution. In addition, each lot is tested for sterility, pH, osmolality, mycoplasma and endotoxin.

Unique, optimized formulation
Gibco® MarrowMAX™ Bone Marrow Medium is an optimized RPMI 1640 medium, supplemented with Fetal Bovine Serum (FBS), gentamicin, and L-glutamine. It also contains a novel human stromal cell conditioned medium that consists of a unique blend of hematopoietic growth factors. Gibco® MarrowMAX™ Bone Marrow Medium does not contain GCT conditioned medium.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® MarrowMAX™ Bone Marrow Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Chromosome Medium 1A (Gibco™)

Gibco® Chromosome Medium 1A is a complete medium for the culture of lymphocytes isolated from bone marrow for cytogenetic studies. Lymphocytes from peripheral blood can also be cultured in Gibco® Chromosome Medium 1A, with the addition of phytohemagglutinin (PHA). The complete formulation is available.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® Chromosome Medium 1A is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.

Neurobasal™-A Medium, minus phenol red (Gibco™)

Neurobasal Medium minus phenol red is a basal medium designed for long-term maintenance and maturation of pure post-natal and adult brain neurons without the need for an astrocyte feeder layer when used with Gibco B-27 supplements. Use this modified formulation of Neurobasal Medium when the presence of phenol red will interfere with your results.

Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.

For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

Sf-900™ II SFM (Gibco™)

Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

ExpiSf™ CD Medium (Gibco™)

ExpiSf CD Medium is a GMP-manufactured, chemically defined, yeastolate-free, serum-free, protein-free, animal origin-free medium specifically designed for high-density growth, transfection, and baculovirus infection of suspension-adapted Sf9 (Spodoptera frugiperda) insect cells.

ExpiSf CD Medium:
• Delivers high reproducibility and lot-to-lot reliability
• Facilitates high-level expression of baculovirus-infected cultures, achieving protein yields three times higher than traditional insect expression platforms
• Supports growth of suspension Sf9 cultures to densities of approximately 2 x 107 cells/mL, with >90% cell viability
• Supports high-density infection of suspension Sf9 cells with recombinant baculovirus
• Supports growth, transfection, and baculovirus infection of Sf9 cells in culture formats from 24-deep well plates to >3 L in shaker flasks
• Is a ready-to-use formulation, with no supplementation required

ExpiSf CD Medium is a core component of the ExpiSf Expression System. For highest level protein expression, the medium is used in combination with a matched protein expression enhancer, ExpiSf Enhancer, which is supplied as part of the ExpiSf Protein Production Kit. Sf21 and High Five cells adapted to other commercially-available serum-free media may be adapted for growth in ExpiSf CD Medium; although, sequential adaptation is required and high-density growth isn’t guaranteed. ExpiSf CD Medium is not recommended for adherent cell cultures. Cells are typically grown in Erlenmeyer-style flasks on a shaker platform in a non-humidified, non-CO2 cell culture incubator.

cGMP manufacturing
ExpiSf CD Medium is manufactured using an ISO 13485-certified process according to 21 CFR 820 medical device current good manufacturing practices (cGMP).

Dry media formulations
For added flexibility, ExpiSf CD Medium can be made in AGT dry format at large scale. For custom media requests, please contact Gibco Custom Media Services.

iPSC Lab Starter Kit (Gibco™)

The Gibco™ iPSC Lab Starter Kit provides researchers initiating pluripotent stem cell research with the required reagents at a significant discount over purchasing them separately. This package is appropriate for labs that are starting from cryopreserved pluripotent stem cells previously adapted to culture in Essential 8™ medium. When starting from cells that are adapted to other feeder-based or feeder-free culture systems, please add the Essential 8™ Adaptation Kit (sold separately).For labs that plan to perform reprogramming of donor-derived fibroblasts , please see the Gibco™ iPSC Lab Starter Kit with Reprogramming.

The iPSC Lab Starter Kit includes the following products for PSC growth and expansion:
• Essential 8™ Medium, Cat. No. A1517001, 6 x 500 mL
• Geltrex™ hESC-qualified Basement Membrane Extract, Cat. No. A1413302, 2 x 5 mL
• Vitronectin (VTN-N), Cat. No. A14700, 4 x 1 mL
• Versene Solution, Cat. No. 15040066, 2 x 100 mL
• Dulbecco’s Phosphate Buffered Saline without Ca2+/Mg2+, Cat. No 14190250, 30 x 500 mL

Medium 200 (Gibco™)

A sterile, liquid medium for the culture of human large vessel endothelial cells. This basal medium requires the addition of LSGS (S-003-10) or LSGS Kit (S-003-K) prior to use.

Protein Expression Medium (PEM) (Gibco™)

• Supports the large-scale, high-density culture of transfected PER.C6® cells in shake flasks, roller bottles, and bioreactors
• Contains no components directly derived from human or animal sources and one low-level, recombinant protein, minimizing the opportunity to introduce adventitious agents
• Formulated without L-glutamine for maximum stability
• Supports parental PER.C6®, HEK 293, and HeLa cell growth in suspension culture, as well as transfected PER.C6® cells for protein expression applications Storage: Store in the dark at 2-8°C. PER.C6® is a registered trademark of Crucell, Holland B.V.