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Sf-900™ II SFM (Gibco™)

Sf-900™ II SFM is a serum-free, protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Sf-900™ II SFM features:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, ready-to-use formulation
• Scalable in bioreactors

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ II SFM achieve maximum cell densities of 9 to 12 x 106 cells/mL, a significant improvement over competitors' formulations and Grace’s medium (see Product Manual). Increases in maximum cell densities of 20–100% are also observed with the Lymantria dispar (Gypsy moth) and Trichoplusia ni (Tn-368; Cabbage Looper) cell lines. Sf-900™ II SFM is capable of supporting the cultures to >20 passages.

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ II SFM is an improved serum-free, protein-free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect virus and rDNA proteins.

Serum-free, protein-free, ready-to-use formulation
Sf-900™ II SFM is a serum-free, protein-free medium that allows for much easier purification of your protein of interest. Sf-900™ II SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media can be subcultured directly into Sf-900™ II SFM, usually without any further adaptation. Cells usually require some adaptation from serum-containing formulations.

Scalable in bioreactors
The utility of Sf-900™ II SFM in larger-scale cell culture systems was demonstrated with a 5 L Celligen™ bioreactor. Successful infections with rAcNPV were carried out producing rβ-Gal and rEPO (see Product Manual).

Product use
Customers using Gibco® Sf-900 II SFM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from us to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Sf-900 II SFM at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements, are certified to ISO 13485, and are registered with the FDA as medical device manufacturers.

Wave Cellbag prefilled with Sf-900™ II Medium (custom product) (Gibco™)

Sf-900 II Serum Free Media, 10L, prefilled in a 20L Series-O Wave Cellbag. Sf-900 II SFM is a protein-free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for large-scale production of recombinant proteins expressed using the baculovirus expression vector system (BEVS). This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines.

Liver Perfusion Medium (1X) (Gibco™)

Liver Perfusion Medium is a buffered, balanced salt solution formulated to cleanse the liver of blood, prevent clotting, and initiate loosening of cell-to-cell contact.

Cryopreserved Hepatocyte Plating Medium (CHPM) (Gibco™)

Cryopreserved Hepatocyte Plating Medium (CHPM) used for plating Gibco® cryopreserved human hepatocytes for optimal attachment and monolayer formation.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Related Link

Learn more about our Hepatic Cell Culture reagents

CTS™ AIM V™ SFM (Gibco™)

GIBCO® AIM V® Medium CTS™ (Therapeutic Grade) is the first commercially available defined, serum-free formulation for proliferation and/or manipulation of T cells and dendritic cells and manufactured in compliance with cGMP. AIM V® Medium CTS™ is an FDA 510(k) cleared device which is intended for human ex-vivo tissue & cell culture processing applications. It contains L-glutamine, 50 µg/ml streptomycin sulfate, and 10 µg/ml gentamicin sulfate.

Keratinocyte Starter Kit (Gibco™)

The Keratinocyte Starter Kit includes Defined Keratinocyte-SFM Medium, growth supplement, and Primary Human Keratinocytes.

PSC Dopaminergic Neuron Differentiation Kit (Gibco™)

The Gibco™ PSC Dopaminergic Neuron Differentiation Kit is a culture media system that enables the differentiation of human pluripotent stem cells (hPSCs) into functional midbrain dopaminergic neurons. Unlike other methods of differentiating hPSCs into dopaminergic neurons, which can be lengthy, ill-defined, and produce a biologically irrelevant phenotype, the PSC Dopaminergic Neuron Differentiation Kit allows you to differentiate hPSCs into authentic midbrain dopaminergic neurons with increased speed and unprecedented scalability, all while retaining proper midbrain phenotype throughout.

The kit is a three-step system used over 35 days, with components that are sequentially added to specify PSCs to the midbrain floor plate, expand floor plate progenitor cells, and differentiate floor plate progenitor cells into functional midbrain dopaminergic neurons. Each component is performance tested to ensure generation of the correct phenotype, and the midbrain dopaminergic neurons have been functionally tested for the ability to produce spontaneous action potential and release of dopamine upon depolarization.

Step 1: Specification of PSCs into midbrain floor plate cells
During central nervous system development, midbrain dopaminergic neurons are derived from a distinct population of cells termed midbrain floor plate cells, which are formed during days 21–28 of gestation and located along the ventral midline of the developing neural tube. Recent reports have focused on the importance of differentiating hPSCs into cells from this region, rather than using a more generic neural stem cell population, in order to create authentic midbrain dopaminergic neurons. The first step in the creation of dopaminergic neurons using the PSC Dopaminergic Neuron Differentiation Kit is the specification of hPSCs into midbrain-specified floor plate progenitor (FP) cells in complete Floor Plate Specification Medium.

Step 2: Create a bankable population of floor plate progenitor cells
Standard methods of creating midbrain dopaminergic neurons involve lengthy protocols that are continuous and not scalable. Using the PSC Dopaminergic Neuron Differentiation Kit, a workable bank of floor plate progenitor cells that maintain midbrain identity is created.

The ability to bank progenitor cells in this step enables:
• A significant increase in resultant DA neuron purity
• The ability to scale experiments beyond current limits
• The ability to distribute cells among peers or thaw for later use

Step 3: Differentiation of floor plate precursor cells to midbrain dopaminergic neurons
The final step of the PSC Dopaminergic Neuron Differentiation Kit protocol involves differentiation of floor plate progenitor cells into midbrain dopaminergic neurons. Dopaminergic neurons generated during this step have been shown to maintain midbrain identity, exhibit a gene expression profile equivalent to that of neurons generated using published protocols, and exhibit functionality as shown by spontaneous action potential and release of dopamine upon depolarization.

Sf-900™ III SFM (Gibco™)

Sf-900™ III SFM is a low-hydrolysate, serum-free, protein-free, animal origin–free insect cell culture medium optimized for the growth and maintenance of Spodoptera frugiperda (Sf9 and Sf21) cells and for recombinant gene expression using the baculovirus and stable insect expression systems. This medium is suitable for suspension and monolayer culture methods and supports growth of other lepidopteran cell lines. Features of Gibco® Sf-900™ III SFM:

• Superior long-term, high-density growth
• Optimized for recombinant protein production
• Serum-free, protein-free, animal origin–free, ready-to-use formulation
• Improved lot-to-lot consistency

Superior long-term, high-density growth
Spodoptera frugiperda (Sf9) cells grown in Sf-900™ III SFM achieve maximum cell densities of 10 to 14 × 106 cells/mL, a significant improvement over Sf-900™ II SFM and other commercially available serum-free media (see figure).

Optimized for recombinant protein production
Traditionally, Grace's medium supplemented with 10% FBS has been used for recombinant protein expression. Sf-900™ III SFM is an improved serum-free, protein-free, animal origin–free medium designed for growth of Sf9 and other lepidopteran cell lines and production of insect viruses and recombinant proteins.

Serum-free, protein-free, animal origin–free, ready-to-use formulation
Gibco® Sf-900™ III SFM is a serum-free, protein-free, animal origin–free medium that allows for much easier purification of your protein of interest. Sf-900™ III SFM is ready to use; it does not require addition of serum, glutamine, or surfactants. Cells adapted to other commercially available serum-free media should be sequentially adapted into Sf-900™ III SFM (see product manual for details).

Improved lot-to-lot consistency
Gibco® Sf-900™ III SFM contains reduced hydrolysate concentrations, improving lot-to-lot consistency over Sf-900™ II SFM (see figure).

cGMP manufacturing and quality system
Gibco® Sf-900 II SFM is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

CD 293 AGT™ Medium (Gibco™)

CD 293 AGT™ is a protein-free, chemically-defined medium in an easy-to-use granular format. CD 293 AGT™ is optimized for the growth of suspension cultures of 293 cells.
Advanced Granulation Technology = AGT

Expi293™ Expression Medium (Gibco™)

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10L in disposable bioreactors
• Does not contain phenol red

Expi293™ Expression Medium is formulated with GlutaMAX™-I reagent. It is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. Expi293™ Expression Medium contains no human or animal-origin components. The medium is not recommended for adherent cell cultures.

CD CHO AGT™ Medium (Gibco™)

CD CHO AGT™ (Advanced Granulation Technology) medium is a protein-free, chemically-defined medium optimized for the growth of Chinese hamster ovary (CHO) cells and expression of recombinant proteins in suspension culture. The AGT™ dry media format is designed to provide increased consistency and productivity across all stages of production from development to commercial manufacturing. CD CHO AGT™ medium contains no proteins or peptide components of animal, plant, or synthetic origin, as well as no undefined lysates or hydrolysates.

When to Use CD CHO Medium
• Your GS CHO cells are recommended to be grown in CD CHO medium
• Your cells have been successfully cultured in CD CHO medium in the past and your process is nearing a regulatory filing (DMF available)

When to Consider Another Gibco® Medium

Consider CD FortiCHO™ medium when:
• The cell line you are using is a transfected CHO K1, GS CHO or CHO-S™ cell line
• Maximum batch culture cell densities and protein titers are needed
• You have time to adapt cells into a new medium in an effort to maximize titers
• Cell health at harvest is a priority for downstream processing

Consider CD OptiCHO™ medium when:
• You are using a transfected CHO cell line other than CHO K1, GS CHO or CHO-S™ cell lines
• Optimization of feeding strategies will be part of your base medium selection testing in an effort to maximize productivity
• Your CHO cell line is “finicky," i.e. hard to grow or adapt

Consider CD DG44 medium when:
• You use parental DG44 or DXB11 CHO cells (dhfr-)

Liquid Media Formulations
Gibco® AGT™ media are also available in a liquid format that offers the same performance and formulation as the AGT™ media format. Gibco® liquid media are manufactured animal origin-free with the same complete, serum-free, protein-free, chemically-defined formulation and performance as their AGT™ media counterparts.

Design Your Own Media Specific to Your Needs
Whether it’s a bench-level experiment or large scale biomanufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco® cGMP Media Custom Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you.

CTS™ OpTmizer™ T Cell Expansion SFM, bottle format (Gibco™)

GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium
• Consistent performance in supporting T cell expansion
• Supports high density T cell culture (e.g., >3x106 CD3+ T cells/mL) in static culture

With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.

Workflow benefits:
• Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs
• More rapid T cell expansion, thus reducing cycle time and improving productivity
• Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload

AmnioMAX™-II Complete Medium (Gibco™)

Gibco® AmnioMAX™ - II Complete Medium is a fully-supplemented medium developed for the short term culture of human amniotic fluid cells for cytogenetic studies and in vitro diagnostic procedures.

Gibco® AmnioMAX™ - II Complete Medium features:

• Ready-to-use format
• Quality and performance testing
• Unique, optimized formulation

Ready-to-use format
Gibco® AmnioMAX™ - II Complete Medium is a frozen, 1X medium, ready to use upon thawing. No supplementation is required. Thawed medium can be stored at 2–8°C for up to ten days.

Quality and performance testing
Every lot of Gibco® AmnioMAX™ - II Complete Medium is performance tested by a certified US reference cytogenetics laboratory to ensure consistently superior performance. Pooled primary human amniotic fluid samples are cultured for six days in Gibco® AmnioMAX™ - II Complete Medium before measuring the total number of colonies and total number of mitotic colonies. In addition, each lot is tested for sterility, pH and osmolality.

Unique, optimized formulation
Gibco® AmnioMAX™ - II Complete Medium contains Fetal Bovine Serum (FBS), gentamicin, and L-glutamine to maximize cell attachment and growth. This optimized medium also has an enhanced buffering system that provides greater pH stability during culture manipulations.

Product Use
For in vitro diagnostic use.

cGMP Manufacturing and Quality System
Gibco® AmnioMAX™ - II Complete Medium is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Keratinocyte-SFM (1X) (Gibco™)

Contains L-glutamine. Keratinocyte-SFM is supplied with prequalified human recombinant Epidermal Growth Factor 1-53 (EGF 1-53) and Bovine Pituitary Extract (BPE) in separate packaging. Components are not sold separately.

Note:
A Keratinocyte-SFM Master File has been submitted to the FDA. Permission to cross-reference the Master File may be obtained by contacting Technical Services or your local Sales Representative.

LHC-8 Medium (1X) (Gibco™)

LHC serum-free media are the most widely used products for culture of bronchial epithelial cells. For maximum flexibility and convenience, we offer these products with or without supplementation.

LHC Basal Medium (1X), liquid

Applications:
Formulated for the growth of bronchial epithelial cells with further supplements.

Recommended storage conditions:
2° to 8°C.

Shelf life:
12 months

Intended use(s)
Research use only



LHC-8 Medium and LHC-9 Medium


Applications:


Engineered specifically for the growth of bronchial epithelial cells such as IB3-1, S9, and C38, BEAS-2B, BZR, BBM, Het-1A, NHBE (normal human bronchial epithelial cells), and BBE (bovine bronchial epithelial cells) for asthma, allergy, lung cancer, cystic fibrosis, pulmonary, and esophageal cancer research without further supplements.


Performance and quality testing:

Performance tested in a growth assay using BEAS-2B cells.

Recommended storage conditions:
-5°C to -20°C.

Intended use(s)
Research use only

Reference(s):
Pawliczak R., et. al. (2001) J Biol Chem 276, 44613. Zhao Y.L., et. al. (2000) Carcinogenesis 21, 2005. Liu X., et. al. (1998) Am J Physiol 274, L58. Hei T.K., et. al. (1997) Environ Health Perspect 105, 1085. Lechner J.F., and LaVeck, M.A. (1985) J Tissue Cult Methods 9, 43.