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Medium 231 Gibco™

Medium 231 is a sterile, liquid culture medium intended for use as one component in a complete culture environment for the growth of normal human vascular smooth muscle cells. When supplemented with SMGS, Medium 231 will support the plating and proliferation of smooth muscle cells at densities between 2.5 x 103 cells/cm2 and 1 x 105 cells/cm2. Additional applications for use may include primary isolation of human smooth muscle cells.

Medium 231 is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. This medium is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air. To support the plating and proliferation of normal human vascular smooth muscle cells, Medium 231 must be supplemented with Smooth Muscle Growth Supplement (SMGS). To induce differentiation of normal human vascular smooth muscle cells, Medium 231 must be supplemented with Smooth Muscle Differentiation Supplement (SMDS).
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