Shop All DNA⁄RNA Storage & Nuclease Control Reagents

RNAlater™-ICE Frozen Tissue Transition Solution (Invitrogen™)

A novel reagent for transitioning frozen tissue to a state enabling easy extraction of high-quality RNA; frozen tissues are simply submerged in Ambion® RNAlater®-ICE and allowed to thaw overnight at –20°C. It is provided in one bottle containing 25 mL. Once thawed, the tissues can be processed like fresh tissues using standard RNA isolation procedures. No more laborious grinding of frozen tissue to safeguard the RNA.

• Process previously frozen tissues like freshly harvested samples
• Thawing tissues in RNAlater®-ICE protects RNA from degradation
• No more tissue pulverizing with mortar and pestle and awkward transfer of powder to tube
• Easily apportion frozen tissue samples for multiple experiments

Quick Freezing Tissues Preserves RNA
Often, tissues that need to be stored prior to RNA isolation are "snap" or "flash" frozen on dry ice or in liquid nitrogen to preserve RNA integrity. RNA in tissue is stable while frozen at –80°C, but thawing the tissue for RNA purification can result in RNA degradation. This is true even if the tissue thaws while in the denaturation solution.

Processing Frozen Tissues is Problematic
Frozen tissues are typically ground with a chilled mortar and pestle in order to maintain RNA quality. Liquid nitrogen must be added to the mortar to keep the sample frozen while it is ground. Powdered tissue can also thaw during transfer to a homogenization vessel. This often results in formation of clumps that do not readily disperse in the lysis solution, resulting in RNA degradation and loss.

Process Frozen Tissue Without Jeopardizing RNA Integrity
RNAlater®-ICE solves all of these problems. Simply submerge frozen tissue samples in 10 volumes of the solution and store overnight at –20°C (the solution will remain as liquid at these temperatures). As the tissue thaws, RNA integrity is protected. Once treated, tissue can be safely stored at 4°C or even at room temperature (for a limited period of time) and can be further dissected or processed prior to homogenization in a standard RNA isolation lysis buffer. Thus the same frozen tissue sample can be used multiple times for different experiments without compromising RNA integrity.

RNaseAlert™ QC System (Invitrogen™)

Ambion® RNaseAlert® QC System is a patent-pending technology detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. Contain sufficient reagents for 5 × 96 high throughput assays.

• Detects as little as 3.5 x 10-7 units (~0.5 pg) of RNase A with a fluorometer
• Simple, straightforward 30 min assay
• Monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product:
RNaseAlert® is also fully compatible with the Ambion® DNaseAlert™ QC System (SKU# AM1970). Since the DNaseAlert™ Substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert®, the two kits can be used simultaneously with a fluorometer for quantitative real-time analyses of both RNases and DNases within a single sample.

RNase AWAY™ Surface Decontaminant (Thermo Scientific™)

Eliminate RNase and DNA from laboratory surfaces. RNase AWAY reduces dependency on carcinogenic DEPC treatments and saves time spent baking glassware.

THE RNA Storage Solution (Invitrogen™)

THE Ambion® RNA Storage Solution is a buffer that provides greater RNA stability than standard 0.1 mM EDTA or TE Buffer. This 1 mM sodium citrate, pH 6.5 +⁄- 0.1 buffer is provided in 10 tubes containing 1.0 mL each.

The last step in every RNA isolation protocol is to resuspend the purified RNA pellet. After painstakingly isolating the RNA, it is crucial that the pellet be suspended and stored in a safe, RNase-free solution. THE RNA Storage Solution has two features that minimize base hydrolysis of RNA: a low pH, and sodium citrate, which is an efficient chelating agent. THE RNA Storage Solution is compatible with all common RNA applications such as reverse transcription, in vitro transcription, northern analysis, and nuclease protection assays. THE RNA Storage Solution is prepared from the purest reagents available, under scrupulously monitored conditions in a state-of-the-art manufacturing facility. It is then subjected to rigorous quality control procedures to guarantee their purity and performance.

DNaseAlert™ QC System (Invitrogen™)

Ambion® DNaseAlert™ QC System is a patent pending technology provides the sensitive detection of DNases in a simple, easy-to-use fluorimetric assay that delivers results in real time. Contains sufficient reagents for 5 × 96 high throughput assays.

• Detect a range of different DNases in minutes
• Certify plastics, enzymes, solutions, and other biomaterials as "DNase-free" prior to DNase-sensitive applications such as PCR
• Combine with RNaseAlert® to quantitatively resolve both RNase and DNase activity simultaneously

The DNaseAlert™ QC System contains a unique DNA substrate tagged with both a fluorescent reporter and a dark quencher. The uncleaved substrate exhibits minimal fluorescence due to the proximity of the fluor and the quencher. When DNases are present, the linkage between the fluor and quencher is severed, which leads to the emission of a bright orange signal upon excitation at the proper wavelength of light. The extent of cleavage of the substrate is proportional to the level of DNase contamination and can be monitored as an increase in fluorescence on a fluorometer.

Fast and Simple Protocol
The DNaseAlert™ QC System protocol is fast and simple. The substrate is reconstituted with TE buffer and then aliquoted into a 96-well plate containing the reaction buffer. Test samples are then added and fluorescence is monitored to determine the results of the assay. Enough substrate is provided for 500 reactions. The DNaseAlert™ QC System also includes both negative and positive controls for accurately identifying contaminated samples. DNaseAlert™ is a kinetically reliable assay. Data can be collected in real time, and the initial velocities are dose-dependent on the amount of input DNase I.

Accessory Product:
DNaseAlert™ was designed to interface seamlessly with the RNaseAlert® QC System (SKU# AM1966) and permit two-color detection of both DNases and RNases in the same sample. The fluorescent reporter linked to the DNaseAlert™ substrate is spectrally distinct from the fluorescent tag on the RNaseAlert® substrate.

RNase AWAY™ Decontamination Reagent (Invitrogen™)

RNase AWAY® Reagent is a ready-to-use solution for eliminating RNase contamination from labware. Apply it evenly over the surface of glassware or plasticware to be treated and then rinse it away with distilled water. Unwanted RNase contamination are eliminated. RNase AWAY® Reagent is nonabrasive, noncarcinogenic, and nonbiologically corrosive.

Performance and quality testing
RNase AWAY® Reagent is functionally tested for the elimination of RNase activity. No detectable RNase activity is observed.

RNAsecure™ RNase Inactivation Reagent (Invitrogen™)

Ambion® RNAsecure (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. 1 mL supplied.
• No post-treatment autoclaving
• Safe, easy-to-use reagent inactivates RNases in solutions
• Inactivation process can be repeated to protect against newly introduced contaminants
• Compatible with downstream procedures, such as RT-PCR and in vitro transcription
• Inactivate RNases in Tris and other solutions that cannot be treated with DEPC RNase contamination in reagents used for RNA isolation and analysis can contribute to experimental inconsistency and, at worst, can cause experimental failure. Traditionally, DEPC has been used to treat solutions that come into contact with RNA. DEPC treatment is time-consuming, possibly hazardous, and only eliminates RNases present at the time of DEPC treatment. In addition, some solutions (e.g., primary amine containing compounds such as Tris) cannot be treated with DEPC at all. Finally, DEPC has to be inactivated by autoclaving post-treatment to prevent it from interfering with downstream enzymatic reactions. RNAsecure Reagent eliminates these problems. Unlike DEPC, RNAsecure can be used on virtually any solution and does not require post-treatment autoclaving. A unique feature of RNAsecure is that reheating after the initial treatment will reactivate the RNase-destroying agent to eliminate any new contaminants. RNAsecure is supplied as a 25X concentrated stock. It is added to solutions as part of reactions (in vitro transcription, RT-PCR, etc.), prior to the addition of enzymes, and heated to 60°C for 10 min to inactivate RNase.

RNaseAlert™ QC System v2 (Invitrogen™)

The RNaseAlert® QC System v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This version contains a substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 5 x 96 (480) high-throughput assays.

Features of RnaseAlert® QC System v2:

• Detects as little as ~0.3 pg of RNase A with a fluorometer
• Simple, straight-forward, 30-minute assay
• Designed to monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ QC System to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product
The RNaseAlert® QC System v2 is fully compatible with the DNaseAlert™ QC System. Since the DNaseAlert™ substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert® substrate, the two kits can be used simultaneously for quantitative real-time analyses of both RNases and DNases within a single sample.

RNaseZap™ RNase Decontamination Wipes (Invitrogen™)

Ambion® RNaseZap® Wipes are RNaseZap® in a convenient towel format. You simply wipe RNases off of surfaces and rinse with RNase-free water. Supplied as 100 pop-up sheets in a single container.
• Convenient towel format
• Completely removes RNase contamination from glass and plastic surfaces Ideal for cleaning work surfaces, pipettors, and equipment that must be RNase-free
• Works immediately on contact; no baking or autoclaving necessary The popular Ambion® RNaseZap® is now provided in a convenient wipe format. RNaseZap® Wipes are designed to eliminate RNase contamination on work surfaces, pipettors and equipment that must be RNase-free. RNaseZap® is a formulation of three ingredients known to be active against RNase. It effectively removes even high levels of RNase contamination that similar products cannot. With thorough rinsing, RNaseZap® leaves no residues that are inhibitory to enzymatic reactions. Note: The components are packaged separately. The wipes and RNaseZap® Solution are loaded into the pop-up dispenser prior to first use.

DNAZap™ PCR DNA Degradation Solutions (Invitrogen™)

DNAZap™ Solutions are two solutions that are innocuous when used alone, but become a potent nucleic acid degrading reagent when mixed. This mixture is able to instantaneously degrade high levels of contaminating DNA and RNA from surfaces. Features of DNAZap™ Solutions:

• Completely degrades contaminating DNA and RNA at the level of PCR sensitivity
• Works on contact
• Degrades nucleic acid, unlike other products on the market that only act as detergents
• Ideal for cleaning PCR tubes, PCR machine surfaces, pipettors, lab benches, lab equipment, and microfuge tubes

Using DNAZap™ Solutions
PCR and related amplification techniques produce large amounts of a target nucleic acid sequence. This exquisite sensitivity creates its own drawbacks, primarily the potential amplification of undesirable, contaminating nucleic acids along with the desired sequence—and sometimes at the expense of the desired sequence. DNAZap Solutions provide a convenient method to decontaminate a variety of surfaces in a matter of minutes. It even effectively eliminates contaminating DNA in PCR tubes without inhibiting subsequent enzymatic reactions. All contaminating nucleic acid is degraded to nucleotides, preventing any chance of false positive amplification.

RNAlater™ Stabilization Solution (Invitrogen™)

RNAlater® RNA Stabilization Solution stabilizes and protects cellular RNA in intact, unfrozen tissue samples, eliminating the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing. Tissue pieces can be harvested and submerged in RNAlater® RNA Stabilization Solution for storage without jeopardizing the quality or quantity of RNA obtained after subsequent RNA isolation. Advantages of using RNAlater® RNA Stabilization Solution:

Effectiveness—stabilize RNA for 1 day at 37°C, 1 week at 25°C, 1 month at 4°C, or indefinitely at -20°C
Simplicity—a single reagent that immediately inactivates RNases and stabilizes RNA within tissues or cells
Convenience—no need to freeze samples in liquid nitrogen or rush samples back to the lab freezer
Mobility—perfect for tissue collection "in the field"
Versatility—compatible with many RNA isolation procedures, including most RNA isolation kits

Applications
RNAlater® RNA Stabilization Solution has been tested on a variety of mammalian tissues, plants, E. coli, Xenopus, fish, and Drosophila. It is ideal for:

• Protecting RNA integrity in tissues rich in RNases
• Collecting samples from different time points without having to process the samples from each time point immediately
• Archiving tissues for future microdissection
• Submerging animal cavities or organs to stabilize RNA during lengthy dissection procedures
• Collecting samples at locations (e.g., hospitals, field sites, the space shuttle) where immediate RNA isolation is not possible
• Shipping samples on wet ice or even at room temperature if shipped overnight

RNAlater® RNA Stabilization Solution procedure
The dissected tissue (less than 0.5 cm in any one dimension) is simply submerged in approximately 5 volumes of RNAlater® solution at room temperature. The solution permeates the cells, stabilizing the RNA. The sample can then be stored indefinitely at -20°C (the tissue does not freeze), at 4°C for up to a month, or at 25°C for up to a week. For RNA isolation, the tissue is simply removed from RNAlater® solution and treated as though it had just been harvested. Most tissues can be transferred directly to a lysis buffer and homogenized. Samples treated with RNAlater® solution and then frozen can be ground with mortar and pestle or thawed and processed like fresh tissue without concern for cell rupture and release of RNases since the RNases have already been inactivated. Cells can be spun out and then added to lysis buffer, or in some cases, RNAlater® solution can be added along with the cells directly to the lysis buffer.

Compatible with a variety of procedures
RNAlater® RNA Stabilization Solution is compatible with one-step RNA isolation methods, such as TRIzol® Reagent; with glass binding methods such as Qiagen's RNeasy™ or the Ambion® RNAqueous® kit; with acid phenol extraction methods such as the Ambion® ToTALLY RNA™ kit; and with methods that use oligo(dT) selection of mRNA, such as the Ambion® Poly(A)Purist™ kit. In-house research, as well recently published independent research, indicates that the use of RNAlater® RNA Stabilization Solution for tissue storage does not affect the outcome of subsequent RNA expression analysis experiments compared to other processing methods.

RNaseAlert™ Lab Test Kit (Invitrogen™)

Ambion® RNaseAlert® Lab Test kit is a Patent-pending technology detects RNase activity in a convenient and sensitive assay that delivers results in real time. Suitable for testing small sample numbers and can be used to ensure that solutions, tubes, tips, etc. are RNase-free; the kit contains sufficient reagents for 25 reactions.

• Detects as little as 3.5 x 10-7 units (~0.5 pg) of RNase A, with a fluorometer or by eye
• Simple, straightforward 30 min assay
• Monitor RNase activity in column fractions during protein purification
• Use with DNaseAlert™ to quantitatively detect both RNases and DNases in the same sample

The RNaseAlert® QC System uses a novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength. Fluorescence can be readily detected by eye upon illumination on a UV box, or with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® Substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® Substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution at 37°C for 30–60 min. The results are then read on a transilluminator or fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

Accessory Product:
The RNaseAlert® QC System is also available for high-throughput assays in a 96-well format (SKU# AM1966). RNaseAlert® is also fully compatible with the Ambion® DNaseAlert™ QC System (SKU# AM1970). Since the DNaseAlert™ Substrate contains a fluorescent tag that is spectrally distinct from RNaseAlert®, the two kits can be used simultaneously with a fluorometer for quantitative real-time analyses of both RNases and DNases within a single sample.

RNaseAlert™ Lab Test Kit v2 (Invitrogen™)

RNaseAlert® Lab Test Kit v2 detects RNase activity in a convenient and sensitive fluorimetric assay that delivers results in real time. This kit is suitable for testing small numbers of sample and ensuring that solutions, tubes, tips, etc. in your lab are RNase-free. This new version contains a more sensitive substrate that is ~60% more sensitive than the previous version. The kit contains sufficient reagents for 25 reactions.

• Detects as little as ~0.3 pg of RNase A with a fluorometer
• Simple, straight-forward 30-minute assay

The RNaseAlert® Lab Test Kit v2 uses a new, more sensitive, novel RNA substrate tagged with a fluorescent reporter molecule (fluor) on one end and a quencher on the other. In the absence of RNases, the physical proximity of the quencher dampens fluorescence from the fluor to extremely low levels. When RNases are present, however, the RNA substrate is cleaved, and the fluor and quencher are spatially separated in solution. This causes the fluor to emit a bright green signal when excited by light of the appropriate wavelength.

Fluorescence can be readily detected with a filter-based or monochromator-based fluorometer. Since the fluorescence of the RNaseAlert® substrate increases over time when RNase activity is present, results monitored with a fluorometer can be evaluated kinetically. The sequence of the RNaseAlert® substrate has been carefully optimized to detect several RNases, including RNase A, RNase T1, RNase I, micrococcal nuclease, S1 nuclease, mung bean nuclease, and Benzonase®.

Rapid and Convenient Protocol
The RNaseAlert® procedure requires just minutes to set up. The lyophilized, fluorescent substrate is reconstituted in the supplied tube and incubated with the test solution for a few minutes. The results are then read on a fluorometer. Samples that noticeably fluoresce compared to the negative control are contaminated and should not be used with RNA. Nuclease-free water and RNase A are provided for use as controls.

ElectroZap™ Electrode Decontamination Solution (Invitrogen™)

Ambion® ElectroZap™ is specially formulated to instantaneously eliminate RNase contamination from electrodes. Supplied in one bottle containing 250 mL.

• Easily rinsed away leaving no residue
• Stable, safe, and non-corrosive
• No need for strong acid or alkali
• Also removes DNases and other proteins

Researchers performing RNA analysis often require the use of a pH meter to prepare their RNase-free solutions. The use of the same pH meter by several laboratory personnel, however, can often lead to problems with RNase contamination. This is especially a problem when preparing solutions that cannot be DEPC-treated or autoclaved. Special precautions must be taken when removing RNase contamination from a pH meter so that the accuracy will be unaffected and there is no residue left by the cleaning solution. Ambion® ElectroZap™ is a non-corrosive, stable cleaning solution that has been specially formulated to clean electrode surfaces in a simple, quick, and efficient manner. It is effective even against high levels of RNase or DNase contamination. ElectroZap® is simply sprayed directly onto the electrode and then rinsed off with RNase-free water. It does not leave behind any residue that may interfere with subsequent enzymatic reactions.

RNAsecure™ Resuspension Solution (1 ml Tube) (Invitrogen™)

Ambion® RNAsecure Resuspension Solution (patents pending) is a unique non-enzymatic reagent that will irreversibly inactivate RNases in solution. It is supplied at working concentration for direct resuspension of RNA pellets. Ten tubes containing 1 mL each are provided.

• No post-treatment autoclaving
• Safe, easy-to-use reagent inactivates RNases in solution
• Inactivation process can be repeated to protect against newly introduced contaminants
• Compatible with downstream procedures, such as RT-PCR and in vitro transcription

RNAsecure™ Resuspension Solution is specifically meant for resuspending RNA pellets that may contain trace amounts of ribonuclease (e.g., after total RNA isolation). Note: it cannot reverse RNA degradation that has already occurred, but can prevent further RNA degradation. To use, the RNA pellet is resuspended in the RNAsecure Resuspension Solution and heated to 60°C for 10 min. It can be reheated to eliminate any RNase contamination introduced at a later time.