Shop All PCR Enzyme Buffers

PCRX Enhancer System Invitrogen™

PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates (1). 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of DNA polymerases such as Taq DNA polymerase and Platinum® Pfx DNA Polymerase.

GeneAmp™ 10X PCR Buffer II & MgCl2 Applied Biosystems™

Applied Biosystems® GeneAmp® 10X PCR Buffer II is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification. The provided MgCl2 solution offers versatility for optimizing PCR.

• Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance
• Available in several convenient sizes
• Separate MgCl2 solution offers versatility

GeneAmp® 10X PCR Buffer II, when diluted 1:10, provides the optimal pH and ionic strength for PCR amplification. GeneAmp® 10X PCR Buffer II is magnesium ion free. A separate MgCl2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

Note: See user's manual or package insert for limited label license and trademark information.
For Research Use Only. Not for use in diagnostics procedures.

SuperFi™ Buffer Invitrogen™

Invitrogen™ SuperFi™ Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction.

For direct loading of PCR products on gels, use SuperFi™ Green Buffer which includes a density reagent and two electrophoresis tracking dyes. The colored buffer does not interfere with the enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion.

More Platinum SuperFi DNA Polymerase products ›

Phusion Green HF Buffer Pack Thermo Scientific™

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

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Phusion HF Buffer Pack
Phusion HF Buffer Pack, detergent-free
Phusion GC Buffer Pack
Phusion GC Buffer Pack, detergent-free
Phusion Green GC Buffer Pack

Phusion GC Buffer Pack Thermo Scientific™

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion GC Buffer Pack, detergent-free
Phusion HF Buffer Pack, detergent-free
Phusion HF Buffer Pack
Phusion Green HF Buffer Pack
Phusion Green GC Buffer Pack

GeneAmp™ 10X PCR Gold Buffer & MgCl2 Applied Biosystems™

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.

The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

GeneAmp 10X PCR Gold Buffer is both magnesium ion-free and gelatin-free. A separate MgCl2 solution is provided. When used together, GeneAmp 10X PCR Gold Buffer and the MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

Taq DNA Polymerase PCR Buffer (10X) Invitrogen™

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl2. It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.

Taq Buffer (10X), with KCl and 15 mM MgCl2 Thermo Scientific™

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer with KCl and 15 mM MgCl2 (B16) includes:
• 100 mM Tris-HCl (pH 8.8 at 25°C)
• 500 mM KCl
• 0.8% (v/v) Nonidet P40
• 15 mM MgCl2

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Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer (10X), with KCl
Taq Buffer (10X), without detergent
Taq Buffer with (NH4)2SO4 (10X)

Phusion Green GC Buffer Pack Thermo Scientific™

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion Green HF Buffer Pack
Phusion HF Buffer Pack
Phusion HF Buffer Pack, detergent-free
Phusion GC Buffer Pack
Phusion GC Buffer Pack, detergent-free

AmpliTaq™ 360 Buffer, 25 mM MgCl2 and 360 GC Enhancer Applied Biosystems™

The Applied Biosystems® AmpliTaq® 360 Buffer Kit is used in conjection with the AmpliTaq 360 DNA polymerase for those few experiments when the reagents supplied with the enzyme are not sufficient for the researcher's needs. When used together with or without the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq® 360 DNA Polymerase delivers 360° coverage for a full range of targets.

* Optimized for the broadest range of targets—from everyday to challenging
* Robust amplification of GC-rich sequences with market-leading 360 GC Enhancer
* Achieves the highest quality sequencing data

Optimized for Easy and Challenging Targets
Challenging targets include AT-rich, GC-rich, primer-dimer forming amplicons, homopolymer repeats, and amplicons that pose sequencing challenges. Amplicons that previously required specialized enzymes and reaction conditions can now be reproducibly amplified with a single reagent under standardized conditions (Figure 1). Competitive benchmarking across more than 40 amplicons distinguishes AmpliTaq Gold® 360 as the best-performing enzyme, ensuring the highest probability of success for the amplification of both everyday and challenging targets (Table 1).

As shown in Figure 1, GC-rich regions are especially poorly amplified with competitor DNA polymerases and the original AmpliTaq Gold, while AmpliTaq Gold 360 provides successful, robust amplification.

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Phire Green Reaction Buffer Thermo Scientific™

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

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Phire Reaction Buffer
Phire Reaction Buffer, detergent-free

GeneAmp™ 10X PCR Buffer Applied Biosystems™

Applied Biosystems® GeneAmp® 10X PCR Buffer I is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification.

• Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance
• Available in several convenient sizes

GeneAmp® 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq® DNA Polymerase.

Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Taq Buffer (10X), without detergent Thermo Scientific™

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer without Detergent (B55) includes:

• 100 mM Tris-HCl (pH 8.8 at 25°C)
• 500 mM KCl

Related Products
Taq Buffer (10X), with KCl
Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer with (NH4)2SO4 (10X)
Taq Buffer (10X), with KCl and 15 mM MgCl2

GeneAmp™ 10X PCR Buffer I, (2 x 75 mL) Applied Biosystems™

Applied Biosystems® GeneAmp® 10X PCR Buffer I is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification.

Optimized for use with AmpliTaq® DNA Polymerase offering maximum PCR performance

• Available in several convenient sizes

GeneAmp® 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq® DNA Polymerase.

Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

AmpFLSTR™ PCR Enhancer Applied Biosystems™

AmpFLSTR™ PCR Enhancer is a reagent that enhances PCR amplification when used with buccal samples collected using the Bode Buccal DNA Collector™ and processed using the GlobalFiler™ Express PCR Amplification Kit. By optimizing primer binding efficiency, PCR Enhancer helps simplify data analysis and reduce time to result.

• Optimized for Bode Buccal DNA Collector samples treated with Prep-n-Go™ Buffer and processed using the GlobalFiler Express PCR Amplification Kit
• Simply add PCR Enhancer to the PCR reaction reagents during amplification setup without pretreatment or incubation steps
• Helps improve first pass success when using the GeneMapper™ ID-X automated genotyping expert software system for data analysis

AmpFLSTR PCR Enhancer incorporates easily into the existing direct amplification workflows of forensic laboratories processing single-source samples.
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