Shop All PCR Enzyme Buffers

Taq Buffer (10X), without detergent (Thermo Scientific™)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer without Detergent (B55) includes:

• 100 mM Tris-HCl (pH 8.8 at 25°C)
• 500 mM KCl

Related Products
Taq Buffer (10X), with KCl
Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer with (NH4)2SO4 (10X)
Taq Buffer (10X), with KCl and 15 mM MgCl2

Phusion Green GC Buffer Pack (Thermo Scientific™)

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion Green HF Buffer Pack
Phusion HF Buffer Pack
Phusion HF Buffer Pack, detergent-free
Phusion GC Buffer Pack
Phusion GC Buffer Pack, detergent-free

Yellow Sample Buffer (40X) (Thermo Scientific™)

Thermo Scientific 40X Yellow Sample Buffer is used with Luminaris Color qPCR Master Mixes to color and track the samples. When using the blue Luminaris Color qPCR Master Mix the qPCR reaction mix is blue before sample addition. After adding the sample the reaction mix turns green, making the overall dispensing of qPCR reaction components quick, easy and error-free. This is particularly important for users of opaque white reaction plates and tubes.

The Yellow Sample Buffer is an aqueous solution of the inert yellow dye (pH 8.0). The buffer is provided as a 40X concentrate and used in 1X concentration in the final reaction (for detailed recommendations refer to the manual of Luminaris Color qPCR Master Mix). The yellow dye does not affect the specificity or sensitivity of qPCR assays. The absorption maximum of the yellow dye is at 413 nm.

Highlights

• Colored sample buffer for easy tracking during pipetting.

GeneAmp™ 10X PCR Gold Buffer & MgCl2 (Applied Biosystems™)

Applied Biosystems GeneAmp 10X PCR Gold Buffer is formulated to provide flexible, efficient activation of AmpliTaq Gold DNA Polymerase, resulting in highly specific, robust PCR amplification.

The ionic strength and pH of GeneAmp 10X PCR Gold Buffer have been optimized to provide a wider activation temperature and time range when used in conjunction with AmpliTaq Gold DNA Polymerase.

GeneAmp 10X PCR Gold Buffer is both magnesium ion-free and gelatin-free. A separate MgCl2 solution is provided. When used together, GeneAmp 10X PCR Gold Buffer and the MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template.

PCRX Enhancer System (Invitrogen™)

PCRx Enhancer System is used in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates (1). 10X PCRx Enhancer Solution with 10X PCRx Amplification Buffer offers higher primer specificity, broader magnesium concentration, broader annealing temperature, and improved thermostability of DNA polymerases such as Taq DNA polymerase and Platinum® Pfx DNA Polymerase.

Taq DNA Polymerase PCR Buffer (10X) (Invitrogen™)

Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 ml of 50 mM MgCl2. It is included with Platinum® Taq, Taq, and the SuperScript® First-Strand Synthesis System for RT-PCR.

Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2 (Thermo Scientific™)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer with (NH4)2SO4 and 20 mM MgCl2 (B34) includes:
• 750 mM Tris-HCl (pH 8.8 at 25°C)
• 200 mM (NH4)2SO4
• 0.1% (v/v) Tween 20
• 20 mM MgCl2

Related Products
Taq Buffer (10X), without detergent
Taq Buffer (10X), with KCl and 15 mM MgCl2
Taq Buffer (10X), with KCl
Taq Buffer with (NH4)2SO4 (10X)

DreamTaq™ Green Buffer (10X) (Thermo Scientific™)

Thermo Scientific™ 10X DreamTaq™ Green Buffer is a proprietary PCR buffer that contains KCl and (NH4)2SO4 at a ratio optimized for robust performance of DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase in PCR and includes MgCl2 at a concentration of 20 mM. In addition, the buffer includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress.

The dyes have excitation peaks at 424 nm and 615 nm. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq™ Buffer (10X) or purifying the PCR product using the GeneJET PCR Purification Kit prior to analysis.

GeneAmp™ 10X PCR Buffer I, (2 x 75 mL) (Applied Biosystems™)

Applied Biosystems® GeneAmp® 10X PCR Buffer I is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification.

Optimized for use with AmpliTaq® DNA Polymerase offering maximum PCR performance

• Available in several convenient sizes

GeneAmp® 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq® DNA Polymerase.

Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

SuperFi™ Green Buffer (Invitrogen™)

Invitrogen™ SuperFi™ Green Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction. SuperFi Green Buffer also includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with the enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation, and restriction digestion. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless SuperFi™ Buffer .

More Platinum SuperFi DNA Polymerase products ›

Phusion HF Buffer Pack (Thermo Scientific™)

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion HF Buffer Pack, detergent-free
Phusion GC Buffer Pack
Phusion GC Buffer Pack, detergent-free
Phusion Green GC Buffer Pack
Phusion Green HF Buffer Pack

Phusion Green HF Buffer Pack (Thermo Scientific™)

Two optimized buffers are available for Thermo Scientific Phusion High-Fidelity DNA Polymerases:

• 5X Phusion HF Buffer (F-518L, F-538L (Phusion Green), F-520L (Detergent-free))
• 5X Phusion GC Buffer (F-519L, F-539L (Phusion Green), F-521L (Detergent-free))

The error rate of Phusion DNA Polymerase in HF Buffer (4.4 × 10-7) is lower than that in GC Buffer (9.5 × 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures. For applications such as microarray or DHPLC, where the DNA templates need to be free of detergents, we recommend using detergent-free reaction buffers.

5X Phusion Green Buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffers do not interfere with the enzyme performance and are compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Related Products
Phusion HF Buffer Pack
Phusion HF Buffer Pack, detergent-free
Phusion GC Buffer Pack
Phusion GC Buffer Pack, detergent-free
Phusion Green GC Buffer Pack

GeneAmp™ 10X PCR Buffer II & MgCl2 (Applied Biosystems™)

Applied Biosystems® GeneAmp® 10X PCR Buffer II is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification. The provided MgCl2 solution offers versatility for optimizing PCR.

• Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance
• Available in several convenient sizes
• Separate MgCl2 solution offers versatility

GeneAmp® 10X PCR Buffer II, when diluted 1:10, provides the optimal pH and ionic strength for PCR amplification. GeneAmp® 10X PCR Buffer II is magnesium ion free. A separate MgCl2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template. Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

AmpliTaq Gold™ 360 Buffer Kit (Applied Biosystems™)

The AmpliTaq Gold® 360 Buffer Kit is used in conjunction with AmpliTaq Gold® 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient. The kit includes 1.5 mL (each) of 10X AmpliTaq Gold 360 Buffer, 25 mM MgCl2, and 3360 GC Enhancer. Typically, 1.5 mL is sufficient for use with 250 U of enzyme.

Taq Buffer with (NH4)2SO4 (10X) (Thermo Scientific™)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer with (NH4)2SO4 (B33) includes:
• 750 mM Tris-HCl (pH 8.8 at 25°C)
• 200 mM (NH4)2SO4
• 0.1% (v/v) Tween 20

Related Products
Taq Buffer (10X), with KCl and 15 mM MgCl2
Taq Buffer (10X), with KCl
Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer (10X), without detergent