Shop All Restriction Enzyme Buffers

Ecl136II, PacI, SacI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Ecl136II, PacI, SacI is the optimal buffer recommended for use with the Ecl136II, PacI, and SacI restriction enzymes and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

FastDigest Buffer (10X) (Thermo Scientific™)

The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix

Benefits:
• Double and multiple digestion in a single buffer with any combination of enzymes.
• No sequential digestions or buffer changes needed.

EcoRI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer EcoRI is the optimal buffer recommended for use with EcoRI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

FastDigest Green Buffer (10X) (Thermo Scientific™)

The 10X FastDigest Green Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Green Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix

Benefits:
• Double and multiple digestion in a single buffer with any combination of enzymes.
• No sequential digestions or buffer changes needed.
• Allows direct loading of reaction mixture on gels.
• Saves pipetting time and eliminates the need for DNA loading dyes.

BamHI-Lsp1109I Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer BamHI, Lsp1109I is the optimal buffer recommended for use with BamHI and Lsp1109I restriction enzymes and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI is the optimal buffer recommended for use with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Eco52I Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Eco52I is the optimal buffer recommended for use with Eco52I restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

BseXI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer BseXI is the optimal buffer recommended for use with BseXI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Buffer B (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer B ensures the optimum reaction conditions for specific restriction enzymes and is premixed with BSA for enhanced stability. It is part of Thermo Scientific Five Buffer System which ensures the optimum reaction conditions for each restriction enzyme. The system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme. AarI and BveI require an oligonucleotide (also supplied with the enzyme), and Esp3I requires DTT.

Reaction Buffer for phi29 DNA Polymerase (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase.

Cfr10I Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Cfr10I is the optimal buffer recommended for use with Cfr10I restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Tango Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Y ensures the optimum reaction conditions for restriction enzymes and is premixed with BSA for enhanced stability. Our Five Buffer System ensures the optimum reaction conditions for each restriction enzyme. This system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

Tango buffer has been designed for double digestions of DNA with conventional restriction enzymes. Visit the Thermo Scientific DoubleDigest tool for more information.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme). Esp3I requires DTT.

Buffer R (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer R ensures the optimum reaction conditions for restriction enzymes and is premixed with BSA for enhanced stability. Our Five Buffer System ensures the optimum reaction conditions for each restriction enzyme. This system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme). Esp3I requires DTT.

BfuI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer BfuI is the optimal buffer recommended for use with BfuI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Anza™ Buffer Set (Invitrogen™)

The Invitrogen™ Anza™ Buffer Set includes both the Anza™ Buffer (10X) and the Anza™ Red Buffer (10X). The Anza buffers are proprietary buffer forumulations for use with the Anza™ Restriction Enzyme Cloning System. All Anza restriction enzymes digest DNA to completion in 15 minutes in the Anza buffers, and all Anza DNA modifying enzymes are fully functional in the Anza buffers.

Benefits:
• One buffer for all restriction enzymes—no buffer look-up
• Multiple digestions simplified with a single buffer
• Direct loading from digestion reaction onto gel with Anza Red Buffer
• Streamlined procedures with Anza DNA modifying enzymes

The Anza 10X Buffer Set is part of the Anza Restriction Enzyme Cloning System. For more information on the system, visit www.thermofisher.com/anza.