Shop All Molecular Biology Enzyme Buffers

Reaction Buffer for phi29 DNA Polymerase (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer for phi29 DNA Polymerase is the optimal buffer recommended for use with highly processive phi 29 DNA polymerase.

Phire Reaction Buffer (Thermo Scientific™)

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

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Phire Green Reaction Buffer
Phire Reaction Buffer, detergent-free

Buffer R (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer R ensures the optimum reaction conditions for restriction enzymes and is premixed with BSA for enhanced stability. Our Five Buffer System ensures the optimum reaction conditions for each restriction enzyme. This system consists of 10X B (blue), G (green), O (orange), R (red), and Tango (yellow) buffers. All restriction enzymes are supplied in color-coded tubes to indicate the recommended reaction buffer. The recommended buffer and/or the universal Tango buffer are supplied with each enzyme.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer. However, some enzymes require additives to achieve 100% activity. For example, AjuI, AlfI, BdaI, BplI, BseMII, FaqI, Eco57I, Eco57MI, Hin4I, and TsoI require S-adenosylmethionine, which is supplied with the enzyme, while AarI and BveI require an oligonucleotide (also supplied with the enzyme). Esp3I requires DTT.

Phire Reaction Buffer, detergent-free (Thermo Scientific™)

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

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Phire Green Reaction Buffer
Phire Reaction Buffer

TaqI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer TaqI is the optimal buffer recommended for use with TaqI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Taq Buffer (10X), with KCl (Thermo Scientific™)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer with KCl (B38) includes:
• 100 mM Tris-HCl (pH 8.8 at 25°C)
• 500 mM KCl
• 0.8% (v/v) Nonidet P40

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Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer (10X), without detergent
Taq Buffer (10X), with KCl and 15 mM MgCl2
Taq Buffer with (NH4)2SO4 (10X)

FastDigest Buffer (10X) (Thermo Scientific™)

The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix

Benefits:
• Double and multiple digestion in a single buffer with any combination of enzymes.
• No sequential digestions or buffer changes needed.

AmpFLSTR™ PCR Enhancer (Applied Biosystems™)

AmpFLSTR™ PCR Enhancer is a reagent that enhances PCR amplification when used with buccal samples collected using the Bode Buccal DNA Collector™ and processed using the GlobalFiler™ Express PCR Amplification Kit. By optimizing primer binding efficiency, PCR Enhancer helps simplify data analysis and reduce time to result.

• Optimized for Bode Buccal DNA Collector samples treated with Prep-n-Go™ Buffer and processed using the GlobalFiler Express PCR Amplification Kit
• Simply add PCR Enhancer to the PCR reaction reagents during amplification setup without pretreatment or incubation steps
• Helps improve first pass success when using the GeneMapper™ ID-X automated genotyping expert software system for data analysis

AmpFLSTR PCR Enhancer incorporates easily into the existing direct amplification workflows of forensic laboratories processing single-source samples.

Phire Green Reaction Buffer (Thermo Scientific™)

The reaction buffer for Thermo Scientific Phire Hot Start II DNA Polymerase is available in three formats: non-colored version with and without detergents (F-524L and F-525L, respectively) or Green version (F-527L) containing a density reagent and two tracking dyes for direct loading of PCR products on gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

The concentration of Mg2+ in the reaction buffers is optimized to work well for most amplicons. The buffer contains 1.5 mM MgCl2 at final reaction concentration. Separate tubes of MgCl2 and DMSO are provided for further optimization.

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Phire Reaction Buffer
Phire Reaction Buffer, detergent-free

Reaction Buffer with MgCl2 for DNase I (10X) (Thermo Scientific™)

Thermo Scientific 10X Reaction Buffer with MgCl2 is used with RNase-free DNase I, an endonuclease that digests single- and double-stranded DNA.

EcoRI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer EcoRI is the optimal buffer recommended for use with EcoRI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

KpnI Buffer (10X) (Thermo Scientific™)

Thermo Scientific KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer. Isoschizomers: Asp718I. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity refer to product specifications.

Yellow Sample Buffer (40X) (Thermo Scientific™)

Thermo Scientific 40X Yellow Sample Buffer is used with Luminaris Color qPCR Master Mixes to color and track the samples. When using the blue Luminaris Color qPCR Master Mix the qPCR reaction mix is blue before sample addition. After adding the sample the reaction mix turns green, making the overall dispensing of qPCR reaction components quick, easy and error-free. This is particularly important for users of opaque white reaction plates and tubes.

The Yellow Sample Buffer is an aqueous solution of the inert yellow dye (pH 8.0). The buffer is provided as a 40X concentrate and used in 1X concentration in the final reaction (for detailed recommendations refer to the manual of Luminaris Color qPCR Master Mix). The yellow dye does not affect the specificity or sensitivity of qPCR assays. The absorption maximum of the yellow dye is at 413 nm.

Highlights

• Colored sample buffer for easy tracking during pipetting.

DreamTaq™ Buffer (10X) (Thermo Scientific™)

Thermo Scientific™ 10X DreamTaq™ Buffer is specifically optimized for PCR using DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase. The buffer contains both KCl and (NH4)2SO4 to provide high specificity of primer annealing at a wide range of MgCl2 concentrations. 10X DreamTaq buffer includes 20 mM MgCl2.

DreamTaq™ Green Buffer (10X) (Thermo Scientific™)

Thermo Scientific™ 10X DreamTaq™ Green Buffer is a proprietary PCR buffer that contains KCl and (NH4)2SO4 at a ratio optimized for robust performance of DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase in PCR and includes MgCl2 at a concentration of 20 mM. In addition, the buffer includes a density reagent and two tracking dyes. The density reagent allows direct loading of PCR products on a gel. The blue dye (migrates with 3 to 5 kb DNA fragments in 1% agarose gel) and the yellow dye (migrates faster than 10 bp DNA fragments in 1% agarose gel) are included for monitoring electrophoresis progress.

The dyes have excitation peaks at 424 nm and 615 nm. For applications that require PCR product analysis by absorbance or fluorescence excitation, we recommend using the colorless DreamTaq™ Buffer (10X) or purifying the PCR product using the GeneJET PCR Purification Kit prior to analysis.