Shop All Molecular Biology Enzyme Buffers

Yellow Sample Buffer (40X) (Thermo Scientific™)

Thermo Scientific 40X Yellow Sample Buffer is used with Luminaris Color qPCR Master Mixes to color and track the samples. When using the blue Luminaris Color qPCR Master Mix the qPCR reaction mix is blue before sample addition. After adding the sample the reaction mix turns green, making the overall dispensing of qPCR reaction components quick, easy and error-free. This is particularly important for users of opaque white reaction plates and tubes.

The Yellow Sample Buffer is an aqueous solution of the inert yellow dye (pH 8.0). The buffer is provided as a 40X concentrate and used in 1X concentration in the final reaction (for detailed recommendations refer to the manual of Luminaris Color qPCR Master Mix). The yellow dye does not affect the specificity or sensitivity of qPCR assays. The absorption maximum of the yellow dye is at 413 nm.

Highlights

• Colored sample buffer for easy tracking during pipetting.

GeneAmp™ 10X PCR Buffer (Applied Biosystems™)

Applied Biosystems® GeneAmp® 10X PCR Buffer I is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification.

• Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance
• Available in several convenient sizes

GeneAmp® 10X PCR Buffer I contains 15 mM MgCl2. When diluted 1:10, it provides the optimal pH and ionic strength for PCR amplification with AmpliTaq® DNA Polymerase.

Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

GeneAmp™ 10X PCR Buffer II & MgCl2 (Applied Biosystems™)

Applied Biosystems® GeneAmp® 10X PCR Buffer II is optimized for use with AmpliTaq® DNA Polymerase, resulting in robust PCR amplification. The provided MgCl2 solution offers versatility for optimizing PCR.

• Optimized for use with AmpliTaq® DNA Polymerase, offering maximum PCR performance
• Available in several convenient sizes
• Separate MgCl2 solution offers versatility

GeneAmp® 10X PCR Buffer II, when diluted 1:10, provides the optimal pH and ionic strength for PCR amplification. GeneAmp® 10X PCR Buffer II is magnesium ion free. A separate MgCl2 solution is provided. When used together, GeneAmp® 10X PCR Buffer II and MgCl2 solution offer more versatility in optimizing the magnesium ion concentration to achieve optimal PCR amplification with any specific set of primers and DNA template. Note: See user's manual or package insert for limited label license and trademark information. For Research Use Only. Not for use in diagnostics procedures.

Eam1105I Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Eam1105I is the optimal buffer recommended for use with Eam1105I restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

AmpFLSTR™ PCR Enhancer (Applied Biosystems™)

AmpFLSTR™ PCR Enhancer is a reagent that enhances PCR amplification when used with buccal samples collected using the Bode Buccal DNA Collector™ and processed using the GlobalFiler™ Express PCR Amplification Kit. By optimizing primer binding efficiency, PCR Enhancer helps simplify data analysis and reduce time to result.

• Optimized for Bode Buccal DNA Collector samples treated with Prep-n-Go™ Buffer and processed using the GlobalFiler Express PCR Amplification Kit
• Simply add PCR Enhancer to the PCR reaction reagents during amplification setup without pretreatment or incubation steps
• Helps improve first pass success when using the GeneMapper™ ID-X automated genotyping expert software system for data analysis

AmpFLSTR PCR Enhancer incorporates easily into the existing direct amplification workflows of forensic laboratories processing single-source samples.

Cfr10I Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Cfr10I is the optimal buffer recommended for use with Cfr10I restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

FastDigest Buffer (10X) (Thermo Scientific™)

The 10X FastDigest Buffer is a proprietary digestion buffer which supports 100% activity of all FastDigest restriction enzymes. DNA/RNA modifying enzymes, such as ligases, phosphatases, kinases and mesophilic DNA polymerases also have 100% activity in FastDigest Buffer. Therefore, enzymes used in downstream applications can be directly added to the FastDigest reaction mix

Benefits:
• Double and multiple digestion in a single buffer with any combination of enzymes.
• No sequential digestions or buffer changes needed.

TaqI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer TaqI is the optimal buffer recommended for use with TaqI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

DreamTaq™ Buffer (10X) (Thermo Scientific™)

Thermo Scientific™ 10X DreamTaq™ Buffer is specifically optimized for PCR using DreamTaq™ DNA Polymerase and DreamTaq™ Hot Start DNA Polymerase. The buffer contains both KCl and (NH4)2SO4 to provide high specificity of primer annealing at a wide range of MgCl2 concentrations. 10X DreamTaq buffer includes 20 mM MgCl2.

BseXI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer BseXI is the optimal buffer recommended for use with BseXI restriction enzyme and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Ecl136II, PacI, SacI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer Ecl136II, PacI, SacI is the optimal buffer recommended for use with the Ecl136II, PacI, and SacI restriction enzymes and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

AmpliTaq Gold™ 360 Buffer Kit (Applied Biosystems™)

The AmpliTaq Gold® 360 Buffer Kit is used in conjunction with AmpliTaq Gold® 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient. The kit includes 1.5 mL (each) of 10X AmpliTaq Gold 360 Buffer, 25 mM MgCl2, and 3360 GC Enhancer. Typically, 1.5 mL is sufficient for use with 250 U of enzyme.

KpnI Buffer (10X) (Thermo Scientific™)

Thermo Scientific KpnI restriction enzyme recognizes GGTAC^C sites and cuts best at 37°C in its own unique buffer. Isoschizomers: Asp718I. Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Highlights

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity refer to product specifications.

AarI-AjiI-Bpu10I-ScaI-PasI Buffer (10X) (Thermo Scientific™)

Thermo Scientific 10X Buffer AarI, AjiI, Bpu10I, ScaI, PasI is the optimal buffer recommended for use with AarI, AjiI, Bpu10I, ScaI, and PasI restriction enzymes and is premixed with BSA for enhanced stability.

To ensure consistent enzyme performance, Thermo Scientific restriction enzyme buffers contain BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations. Multiple freeze-thaw cycles of the buffers will not cause BSA precipitation.

Thermo Scientific restriction enzymes exhibit 100% of their certified activity in the recommended buffer.

Taq Buffer with (NH4)2SO4 (10X) (Thermo Scientific™)

Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA Polymerases (both recombinant and native). Available in different compositions and also without detergents.

10X Taq Buffer with (NH4)2SO4 (B33) includes:
• 750 mM Tris-HCl (pH 8.8 at 25°C)
• 200 mM (NH4)2SO4
• 0.1% (v/v) Tween 20

Related Products
Taq Buffer (10X), with KCl and 15 mM MgCl2
Taq Buffer (10X), with KCl
Taq Buffer (10X), with (NH4)2SO4 and 20 mM MgCl2
Taq Buffer (10X), without detergent