Shop All Other Protein Analysis Kits

Pro-Detect™ Rapid DYKDDDDK-His Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid DYKDDDDK-His Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of DYKDDDDK-His double-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the DYKDDDDK-His tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect DYKDDDDK-His-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

Pro-Detect™ Rapid His Competitive Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid His Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of poly-histidine (His)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to His tag to provide a qualitative detection within ten minutes. This assay can be used to detect His-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.

Pro-Detect™ Rapid Antibody Isotyping Assay Kit, mouse (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Mouse is a single-use, dipstick lateral flow kit for quick, easy determination of mouse monoclonal antibody class and subclass identity. This antibody isotyping assay uses membrane-based strips coated with dye-bound conjugated antibodies that provide a visual, color readout of the monoclonal antibody isotype within ten minutes. The kit determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, as well as light-chain identity (kappa vs. lambda light chains).

Features of the Pro-Detect Rapid Antibody Isotyping Assay Kit:
Long shelf life—stable for at least one year at 4°C
Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip
Fast—within 5–10 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to standard ELISA-based isotyping assays

Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.

Determining the class and subclass of a monoclonal antibody is useful in planning the best immunoglobulin purification method. For example, mouse IgA and IgM are best purified by size (i.e., gel exclusion) or using immunoaffinity separation columns. Mouse IgG2a and IgG2b are purified with immobilized Protein A at pH 7–8, while mouse IgG1 binds best to Protein A at pH 8–9. Immunoglobulin that contains kappa light chains can be purified using immobilized Protein L.

The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 µL ascites fluid, 2–22 µL of cell culture supernatant or 1.5 ng of purified antibody.

Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Glycoprotein Standards Set includes six purified standards (two negative and four positive controls) intended for use with any glycoprotein carbohydrate determination protocol.

Related Products
Pierce™ Glycoprotein Carbohydrate Estimation Kit

Pro-Detect™ Rapid Human Fc Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid Human Fc Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of human Fc-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the human Fc tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect human Fc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

Pro-Detect™ Rapid SUMO Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid SUMO Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of small ubiquitin-like modifer (SUMO)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the SUMO tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect SUMO-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

Pierce™ Peroxide Assay Reagent B (Thermo Scientific™)

Thermo Scientific Pierce Peroxide Assay Reagent B is a formulation consisting of 125µM xylenol orange in water with sorbitol. This formulation is one of the component of Quantitative Peroxide Assay Kit used for detecting and measuring hydrogen peroxide levels based on the oxidation of ferrous to ferric ion in the presence of xylenol orange, present in the biological and other liquid samples.

Features of Peroxide Assay Reagent B:

Convenient—no proprietary reagents involved and remove the variability, difficulty and added expense of formulating the reagents yourself
Aqueous-compatible formulations— the formulation includes sorbitol, which provides enhanced sensitivity with non-lipid samples

Applications of Pierce Quantitative Peroxide Assay Kits:
• General hydrogen peroxide (H2O2) measurement in protein samples
• Quantitation of peroxides in detergents
• Assessment of the effects of peroxides on cellular activity
• Determination of protein glycation; Measurement of peroxide accumulation in lipids

In these assay kits, hydroperoxides convert the Fe2+ to Fe3+ at acidic pH. With the aqueous-compatible formulation, peroxide first reacts with sorbitol, converting it to a peroxyl radical, which in turn initiates Fe2+ oxidation to Fe3+. In the lipid compatible formulation, the peroxide converts the Fe2+ to Fe3+ directly. In a sulfuric acid solution, the Fe3+ complexes with the xylenol orange (XO) dye to yield a purple product having an absorbance maximum at 560nm. Peroxide levels in test samples can be determined by calculation from the know extinction coefficient of the XO-Fe complex or by reference to a standard curve prepared with hydrogen peroxide solution.Most proteins do not interfere with these assays, although some metal chelators may require use of a blank (i.e., control) to mitigate these effects.

Related Products
Pierce™ Quantitative Peroxide Assay Kit (Aqueous)
Pierce™ Quantitative Peroxide Assay Kit (Lipid)
Pierce™ Peroxide Assay Reagent A
Pierce™ Peroxide Assay Reagent C

Pro-Detect™ Rapid DYKDDDDK Competitive Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid DYKDDDDK Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of DYKDDDDK (commonly known as "FLAG")-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to DYKDDDDK tag to provide a qualitative detection within ten minutes. This assay can be used to detect DYKDDDDK-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.

Pro-Detect™ Rapid Antibody Isotyping Assay Kit, rat (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid Antibody Isotyping Assay Kit - Rat is a single-use, dipstick lateral flow kit for quick, easy determination of rat monoclonal antibody class and subclass identity. This antibody isotyping assay uses membrane-based strips coated with dye-bound conjugated antibodies that provide a visual, color readout of the monoclonal antibody isotype within ten minutes. The kit determines rat IgG1, IgG2a, IgG2b , and IgG2c.

Features of the Pro-Detect Rapid Antibody Isotyping Assay Kit:
Long shelf life—stable for at least one year at 4°C
Simple workflow—dilute antibody sample using provided diluent and dip in the lateral flow strip
Fast—within 5–10 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples down to low ng/mL concentrations
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to standard ELISA-based isotyping assays

Each Pro-Detect Rapid Antibody Isotyping assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to a test tube or microplate well and dipping in a lateral flow strip. Gold conjugates embedded in the strip membrane form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated regions of the membrane. Results are displayed as a red band above the printed class or sub-class description, indicating the antibody isotype present.

Determining the subclass of a rat antibody is important when selecting the best purification method. IgG2a, IgG2b, and IgG2c can be purified with Protein A columns at a pH of 7–8. IgG1 isotypes bind best to Protein A at a slightly higher pH of 8–9.

The Pro-Detect Rapid Antibody Isotyping Assay Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex bead-based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5 µL ascites fluid, 2–22 µL of cell culture supernatant or 1.5 ng of purified antibody.

Pro-Detect™ Rapid AVI Competitive Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid AVI Competitive Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of AVI (sequence: GLNDIFEAQKIEWHE)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to AVI tag to provide a qualitative detection within ten minutes. This assay can be used to detect AVI-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid competitive assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for qualitative results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid competitive assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. In the presence of target protein tag, the dye-bound conjugated antibodies will bind to the tagged protein present in the sample instead of the protein tag bound at the test lines. Therefore, in a positive result, a competitive lateral flow assay will show decreasing intensity of the visible test lines until all test lines have disappeared. When there is no target protein tag present, the dye-bound conjugated antibodies specific to the target will bind at the test line(s), resulting in visual test lines in a negative result.

Pierce™ Rapid Antibody Isotyping Kit plus Kappa and Lambda - Mouse (Thermo Scientific™)

The Thermo Scientific Pierce Rapid Mouse Antibody Isotyping Kit Plus Kappa and Lambda is a single-use, cassette-based kit for 5-minute lateral-flow assay determination of mouse monoclonal antibody class and subclass identity.

This antibody isotyping kit uses cartridges that provide a color-readout of the monoclonal antibody isotype within five minutes after pipetting a small amount of diluted culture supernatant or ascites fluid sample into the sample well. The kit determines mouse IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, as well as light-chain identity (kappa vs. lambda light chains).

Features of the Pierce Rapid Mouse Antibody Isotyping Kit:

Long shelf life—stable for at least one year at room temperature
Single-step—add diluted antibody sample to the loading-well of cassette
Fast—within 5 minutes a band appears that indicates the antibody isotype
Sensitive—detect antibodies in tissue culture media or ascites fluid samples at any concentration greater than 10 ng/mL
Specific—assay does not cross-react with fetal bovine serum (FBS)
Reliable—results are similar to the standard ELISA-based isotyping assays

Each Pierce Rapid Isotyping Kit assay is performed by simply adding a properly diluted tissue culture supernatant or mouse ascites sample to the well of the small cassette. Gold conjugates embedded in the cassette form specific class- and subclass-soluble complexes with the antibodies in the sample. These complexes travel the length of the membrane and are resolved on the anti-isotype and class-specific antibody-impregnated membrane. Results are displayed as a red band indicating the antibody isotype or subclass.

Determining the class and subclass of a monoclonal antibody is useful in planning the best immunoglobulin purification method. For example, mouse IgA and IgM are best purified by size (i.e., gel exclusion) or using immunoaffinity separation columns. Mouse IgG2a and IgG2b are purified with immobilized Protein A at pH 7-8, while Mouse IgG1 binds best to Protein A at pH 8-9. Immunoglobulin that contains kappa light chains can be purified using immobilized Protein L.

The Pierce Rapid Isotyping Kit is compatible with both tissue culture supernatant and mouse ascites fluid, and is more sensitive than conventional latex-bead based dipstick assays and much faster than ELISA-based isotyping assays. The kit requires only 0.5µL ascites fluid, 5 to 55µL of cell culture supernatant or 1.5ng of purified antibody.

Related Products
Rapid ELISA Mouse mAb Isotyping Kit
Pierce™ Rapid Antibody Isotyping Kit - Mouse

Pierce™ Cell Surface Biotinylation and Isolation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Cell Surface Protein Biotinylation and Isolation Kit enables the selective biotinylation, solubilization, and enrichment of plasma membrane proteins.

Features of the Cell Surface Protein Biotinylation and Isolation Kit include:
Improved performance—kit and protocol have been re-designed to improve enrichment and extraction of biotinylated plasma membrane proteins while minimizing intracellular protein contamination
Optimized—protocol has been refreshed to reduce processing time and the number of steps, and reagent formulations optimized for improved performance
Compatible—reagents have been verified with suspension and adherent cell lines, with additional instructions provided for MS sample preparation
Validated applications—reagents and procedures are compatible with western blotting and MS applications

This easy-to-use kit provides all of the necessary components and procedure for optimal labeling and subsequent isolation of cell surface proteins. Buffers are supplied pre-formulated to produce consistent results. Mammalian cells are first labeled with EZ-Link Sulfo-NHS-SS-Biotin, a thiol-cleavable amine-reactive biotinylation reagent. Cells are subsequently lysed and the labeled proteins are captured with NeutrAvidin Agarose. Dithiothreitol (DTT) is used in the elution to reduce the disulfide bonds in the biotin label, resulting in the release of the bound proteins without the biotin label. This kit contains sufficient reagents for eight experiments, consisting of two 85-95% confluent 15-cm dishes or four 75-cm2 flasks.

Cell surface proteins represent a key subset of cellular proteins and play major roles in signal transduction, cell adhesion, and ion transport. These proteins are challenging to efficiently extract and isolate due to their multiple membrane-spanning domains. Often, the harsh detergents and conditions necessary for efficient extraction result in denaturation and contamination of plasma membrane proteins after isolation. As an alternative, this kit utilizes a membrane-impermeable, amine-reactive, sulfo-NHS-SS-biotin to efficiently label cell surface proteins with accessible lysines. The lysis, capture, wash, and elution conditions have been optimized for efficient enrichment and isolation while minimizing contamination of intracellular proteins.

The protocol has been optimized for use with diverse mammalian cell lines, including adherent and suspension cells, and is useful for differential expression analysis between treated and non-treated cells or between one or more cell lines. Selectivity and efficiency of the cell surface protein isolation has been verified by western blotting and LC-MS analysis.

Pierce™ Glycoprotein Carbohydrate Estimation Kit (Thermo Scientific™)

The Thermo Scientific Pierce Glycoprotein Carbohydrate Estimation Kit enables the amount of protein glycosylation to be measured as the percent of total purified protein mass.

Features of the Glycoprotein Carbohydrate Estimation Kit:

Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
Semi-quantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
Simple procedure—completed in less than 75 minutes; instructions include microplate and test tube protocols
Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

The Glycoprotein Carbohydrate Estimation Kit includes six purified glycoprotein standards and the required assay reagents to effectively estimate the amount of oxidizable glycosylation (percent carbohydrate by weight) of purified protein samples. Sugar groups in the glycoprotein sample are first oxidized with sodium meta-periodate to produce detectable aldehyde groups. Then the sample is reacted with the colorimetric Glycoprotein Detection Reagent. Absorbance at 550 nm of the resulting purple reaction product is measured with a spectrophotometer or plate reader. Finally, the carbohydrate content in the glycoprotein is calculated by comparison to results from the five glycoprotein standards that are included in the kit.

Applications
• Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
• Characterize and classify purified protein fractions from an affinity procedure
• Assess sugar and carbohydrate contamination in non-glycosylated protein samples

Related Products
Glycoprotein Standards Set for Pierce™ Glycoprotein Carbohydrate Estimation Kit

Pro-Detect™ Rapid Rabbit Fc Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid Rabbit Fc Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of rabbit Fc-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the rabbit Fc tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect rabbit Fc-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.

Pro-Detect™ Rapid GST Assay Kit (Thermo Scientific™)

The Thermo Scientific Pro-Detect Rapid GST Assay Kit is a single-use, dipstick lateral flow kit for quick, easy detection of glutathione S transferase (GST)-tagged proteins in protein expression and purification systems. This lateral flow assay uses membrane-based strips coated with dye-bound conjugated antibodies specific to the GST tag to provide a visual, color readout for detection within ten minutes. This assay can be used to detect GST-tagged proteins directly from cell culture media (supernatant) or cell lysates without any special instrumentation or handling.

Features of the Pro-Detect Rapid assay kits:
Easy to adopt—lateral flow strips can be used early in and throughout expression to monitor and confirm expression of desired protein-tagged target
Simple, fast method—simply dilute sample using provided diluent and dip in the lateral flow strip for visual results within 5-10 minutes
Sensitive—detect protein tags in supernatant or cell lysate samples down to low µg/mL concentrations
Specific—assay does not cross-react with common lysis and protein preparation reagents
Reliable—results are similar to other antibody immunoassays, including western blot and ELISA
Long shelf life—stable for at least one year at 4°C

Each Pro-Detect Rapid assay is performed by simply adding a properly diluted tissue culture supernatant, lysate, or purified protein sample to a test tube or microplate well and dipping in a lateral flow strip. Gold-conjugated detection antibodies embedded in the strip form complexes with the tagged protein in the sample. These complexes travel the length of the strip membrane and bind at the test and control lines. Results are displayed as red bands.