Shop All Sequencing Buffers & Reagents

5500 W Buffer E (Applied Biosystems™)

5500 W Buffer E is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer E consists of 5500 W Buffer E Part 1 and 5500 W Buffer E Part 2, which need to be mixed prior to running on the instrument. 5500 W Buffer E contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.

SOLiD™ Buffer E, Double (Applied Biosystems™)

The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. SOLiD™ Buffer E, Double (PN 4463016) consists of SOLiD™ Buffer E Part 1 and SOLiD™ Buffer E Part 2, which need to be mixed prior to running on the instrument. SOLiD™ Buffer E, Double contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane.

These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840). Supplemental buffers can be used to replace components of the main buffer kit. Each double size has sufficient buffer for covering two FlowChips.

dGTP BigDye™ Terminator v1.0 & v3.0 5X Sequencing Buffer (Applied Biosystems™)

This sequencing buffer is optimized for use with BigDye® Terminator v1.0 or v3.0 cycle sequencing kits.

For Research Use Only. Not for use in diagnostics procedures.

R4A (25% Trifluoroacetic Acid in Water) (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, S4B, 200 mL, R1, 5% PITC in N-Heptane, 40 mL, R3, Trifluoroacetic Acid, 40 mL, S3, N-Butyl Chloride, 200 mL, Solvent A3, 3.5% THF⁄Water, 960 mL

For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ Buffer A, Double (Applied Biosystems™)

The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. SOLiD™ Buffer A, Double (PN 4463024) consists of SOLiD™ Buffer A Part 1 and SOLiD™ Buffer A Part 2, which need to be mixed prior to running on the instrument. SOLiD™ Buffer A, Double (PN 4463024) contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane.

These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840). Supplemental buffers can be used to replace components of the main buffer kit. Each double size has sufficient buffer for covering two FlowChips.

Solvent A3, 3.5% Tetrahydrofuran in Water, 450 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.

Solvent B2, Isopropanol & Acetonitrile (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, 5% PITC in N-Heptane, Trifluoroacetic Acid, 25% Trifluoroacetic Acid in Water, Ethyl Acetate, N-Butyl Chloride, 3.5% THF⁄Water

For Research Use Only. Not for use in diagnostics procedures.

R1 (5% PITC in N-Heptane) (Applied Biosystems™)

5% PITC (phenylisothiocynate) in heptane is used for N-terminal protein sequencing applications. PITC is a derivatization reagent used to tag amino acids in the protein sequencer and amino acid analyzer. An optimal PITC concentration is required to ensure efficient amino acid coupling with minimal background contribution from excess reagent. This reagent is used in conjunction with other protein sequencing reagents.

5500 W Buffer H (Applied Biosystems™)

5500 W Buffer H is used for paired-end sequencing on the 5500 W Series genetic analyzers. 5500 W Buffer H contains sufficient reagents to run up to 360 cycles, or 60 cycles per lane for 6 lanes.

Anode Buffer Container (ABC) 3500 Series (Applied Biosystems™)

The anode buffer container (ABC) contains 1x running buffer to support all electrophoresis applications on the Applied Biosystems 3500 series Genetic Analyzers. It is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat sealed with a plastic film, which should be removed prior to direct installation on to the instrument. Each package includes four individual containers.

For Research Use Only. Not for use in diagnostics procedures.

BigDye™ Terminator v1.1 & v3.1 5X Sequencing Buffer (Applied Biosystems™)

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits.

For Research Use Only. Not for use in diagnostics procedures.

Procise™ cLC Reagent Kit, for 500 Cycles (Applied Biosystems™)

This kit contains reagents for Procise® N-terminal protein sequencing (500 cycles).

For Research Use Only. Not for use in diagnostics procedures.

TFA HPLC Grade, 10 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.

SequenceRx Enhancer Solution A (Invitrogen™)

SequenceRx Enhancer Solution A is a novel reaction co-solvent that facilitate sequencing through problematic GC-rich regions,direct repeats, inverted repeats, hairpins, and/or short nucleotide repeats. The component is added to the reaction mixture, and the mixture is incubated in a thermocycler as usual. Early terminations and/or low signal strength aretypically improved within the troublesome region, permitting additional sequence data to be obtained. SequenceRx Enhancer Solution A is fully compatible with BigDye terminator, dRhodamine dye terminator, and rhodaminedye terminator chemistries and all sequencing platforms.

Procise™ Reagent Kit B, for 500 Cycles (Applied Biosystems™)

This kit contains reagents for Procise® N-terminal protein sequencing.

For Research Use Only. Not for use in diagnostics procedures.