Shop All Sequencing Buffers & Reagents

5500 W Buffer H (Applied Biosystems™)

5500 W Buffer H is used for paired-end sequencing on the 5500 W Series genetic analyzers. 5500 W Buffer H contains sufficient reagents to run up to 360 cycles, or 60 cycles per lane for 6 lanes.

5500 W Buffer G (Applied Biosystems™)

5500 W Buffer G is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer G contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.

5500 W Buffer E (Applied Biosystems™)

5500 W Buffer E is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer E consists of 5500 W Buffer E Part 1 and 5500 W Buffer E Part 2, which need to be mixed prior to running on the instrument. 5500 W Buffer E contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.

Procise™ cLC Reagent Kit, for 500 Cycles (Applied Biosystems™)

This kit contains reagents for Procise® N-terminal protein sequencing (500 cycles).

For Research Use Only. Not for use in diagnostics procedures.

Procise™ HT Reagent Kit, for 1000 Cycles (Applied Biosystems™)

This kit contains reagents for Procise® N-terminal protein sequencing (1000 cycles).

For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ Buffer C, Double (Applied Biosystems™)

SOLiD™ Buffer C, Double contains sufficient reagents to run 950 to 1632 cycles, up to 136 cycles per lane. The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840).

SOLiD™ FWD Buffer, Double (Applied Biosystems™)

SOLiD™ FWD Buffer, Double is used for fragment, mate-paired, and paired-end runs, for ligation sequencing in the forward direction. This buffer must be used with the SOLiD™ FWD SR Kit. This buffer contains sufficient material to run 900 to 1212 cycles, or up to 101 cycles per lane.

The SOLiD™ Buffers are used for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. The “Main" kits are the primary kits necessary for typical sequencing runs.

Double-sized buffers have sufficient reagents for covering two FlowChips.

For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ Run Cycle Buffer Kit, Double (Applied Biosystems™)

SOLiD™ Buffers are used for ligation sequencing on the 5500⁄5500xl Genetic Analyzers. The SOLiD™ Run Cycle Buffer Kit (Double) contains a set of six buffers required during all types of sequencing runs for ligation sequencing in the forward and reverse directions. The kit consists of SOLiD™ Buffers A, B, C, E, F, and G.

SOLiD™ Buffers A, B, and E are provided in two parts, and components must be mixed before use. SOLiD™ Buffer F is provided as a 10X solution and requires dilution to 1X before pouring into its reagent tank. SOLiD™ Buffer G is poured into its reagent tank before use.

The kit contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane.

For Research Use Only. Not for use in diagnostics procedures.

S2B, Ethyl Acetate, 450 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, Procise® Sequencing System Model 491 and Model 494, 5% PITC in N-Heptane, Trifluoroacetic Acid, N-Butyl Chloride, 3.5% THF⁄Water, Isopropanol and Acetonitrile

For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ FlowChip Deposition Buffer 1 (Applied Biosystems™)

The SOLiD™ FlowChip Deposition Buffer 1 is required for depositing templated beads onto a SOLiD™ FlowChip for sequencing on the 5500⁄5500xl Genetic Analyzer.

Store the SOLiD™ FlowChip Deposition Buffer 1 at 2°C to 8°C until ready for use.

The SOLiD™ FlowChip Deposition Buffer 1 is also included in the SOLiD™ FlowChip Deposition Kit.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.

SequenceRx Enhancer Solution A (Invitrogen™)

The SequenceRx Enhancer System is designed to improve the length and quality of sequencing data from difficult sequencing templates. It is a panel of seven co-solvents which are added individually to automated fluorescent DNA sequencing reactions. This system is not designed for use on long stretches of poly A.

The SequenceRx Enhancer System:

• Is useful on templates containing regions with secondary structure such as high GC-content, small nucleotide repeats, hairpins, direct repeats, and inverted repeats
• Requires no change in protocols-the SequenceRx Solution replaces part of the water added to the reaction mixture
• Is compatible with all sequencing chemistries and platforms
• Uses one reaction to determine the sequence for all four nucleotides (100 reactions equal 100 templates)

310 and 31xx Running Buffer, 10X (Applied Biosystems™)

The concentrated Running Buffer is used for electrophoresis on Applied Biosystems® 310, 3100, 3100-Avant™, 3130, and 3130xl Genetic Analzyers.

Note: For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ Buffer G, Double (Applied Biosystems™)

SOLiD™ Buffer G, Double contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane. The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840). Supplemental buffers can be used to replace components of the main buffer kit. Each double size has sufficient buffer for covering two FlowChips.

Anode Buffer Container (ABC) 3500 Series (Applied Biosystems™)

The anode buffer container (ABC) contains 1x running buffer to support all electrophoresis applications on the Applied Biosystems 3500 series Genetic Analyzers. It is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat sealed with a plastic film, which should be removed prior to direct installation on to the instrument. Each package includes four individual containers.

For Research Use Only. Not for use in diagnostics procedures.

R4A (25% Trifluoroacetic Acid in Water) (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, S4B, 200 mL, R1, 5% PITC in N-Heptane, 40 mL, R3, Trifluoroacetic Acid, 40 mL, S3, N-Butyl Chloride, 200 mL, Solvent A3, 3.5% THF⁄Water, 960 mL

For Research Use Only. Not for use in diagnostics procedures.