Shop All Forensic Testing Kits

AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Applied Biosystems™)

Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

Features of the AmpFLSTR Identifiler Plus PCR Amplification Kit include:

• Amplifies the same loci as the Identifiler kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler, Identifiler Direct, Profiler Plus, COfiler and SGM Plus kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts

Other Improvements and Benefits
The improvements in the Identifiler Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler kit. In addition, the Identifiler Plus Master Mix includes AmpliTaq Gold DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.

None of the formulation enhancements included in the Identifiler Plus kit have required modifications to the Identifiler Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler kit has been designed to work in conjunction with the Identifiler Plus kit, utilizing miniSTR technology to help maximize recovery of information.

The Identifiler Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler, Identifiler Direct, Profiler Plus, COfiler or SGM Plus kits, and facilitate comparison with existing DNA databases.

The Confidence to Do More
The Identifiler Plus kit is part of a new generation of AmpFLSTR kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.

Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ Express PCR Amplification Kit (Applied Biosystems™)

The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express Kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.

Key Features:

• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect and Identifiler primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

NGM Detect™ PCR Amplification Kit (Applied Biosystems™)

The Applied Biosystems NGM Detect PCR Amplification Kit is the first 6-dye STR kit designed specifically for those laboratories using the European Standard Set (ESS) of loci. The kit offers excellent sensitivity and provides an alternate marker configuration to the well-established NGM SElect PCR Amplification Kit format to maximize information recovery, even from degraded casework samples.

As with all Applied Biosystems STR kits, the NGM Detect kit is ISO18385 Forensic DNA Grade, giving you piece of mind when choosing this kit for your casework samples.

The key features of NGM Detect kit are:
Increased database searches by virtue of prioritization of markers with the highest representation in historic databases
Optimized positioning of SE33 in the sub-350 bp range to support mixture analysis
Dual amplification compatibility when partnered with the NGM SElect kit
High sensitivity through a new master mix formulation coupled with the ability to add up to 15 μL of sample to the reaction
Fast PCR cycle time—<60 min
Additional Y marker enabling gender confirmation
Integrated Quality Control System to verify reaction set up and assess the presence of inhibition in the sample

Increased database searches
The European Standard Set (ESS) of loci was established to enable cross-border information sharing and was composed of seven SGM/SEFiler Plus markers. The ESS was expanded to 12 loci in 2009 to include more markers to enable wider compatibility between neighboring countries.

Because of this, a large number of historic crime-scene profiles contained in databases consist of SGM/SEFiler Plus marker data. When dealing with challenging casework, such as cases involving degraded samples, the information recovery is limited. In this situation, recovering the SGM/SEFiler Plus markers is key for producing a partial DNA profile that has maximum overlap with older data contained in databases, giving a higher probability of a match and providing valuable investigative leads.

The NGM Detect kit takes full advantage of the space afforded by 6 dyes and features optimized marker positioning, placing the SGM/SEFiler Plus markers in the low molecular weight (LMW) range to help ensure the highest compatibility with historic datasets and the possibility of a higher chance of generating investigative leads.

Optimized positioning of SE33
The highly polymorphic SE33 marker is useful for mixture analysis but extends up to 500 bp in most STR kits. As a result, it is one of the first markers to drop out in degraded samples. In the NGM Detect kit, SE33 extends to a maximum of 351 bp to help to significantly increase the chances of recovery of this highly informative marker in challenging samples.

Dual amplification compatibility
Dual amplification, where two different STR kits are used to amplify a sample, is a strategy that can be used to confirm or extend a DNA profile result for increased confidence in the final result in the former and to obtain additional information in the latter.The NGM Detect kit is an advanced partner for the NGM SElect kit, featuring the same loci, but with different primer sequences and different marker placement. When using the two kits in combination, different primer sequences provide confidence that the consensus profile generated reflects the ‘true’ genotype of the sample. The complimentary marker positioning places mini-STR loci in the NGM Detect kit in the high molecular weight (HMW) range in the NGM Select kit and vice versa, meaning that additional alleles can be recovered from degraded samples when using the two kits in combination. Together the NGM Detect and NGM Select system delivers 12 independent mini-STRs.

Integrated Quality Control System
The IQC assay consists of two synthetic sequences not found in nature with primers specific for each of the targets. It provides positive confirmation that the assay is functioning as expected in a given run.

This system is particularly useful to confirm the validity of negative results and can also be used to distinguish samples that are degraded versus those that contain PCR inhibitors, supplementing data analysis and informing downstream processing decisions.

Experience matters
The NGM Detect kit is part of a fully integrated and validated forensic DNA workflow backed by superior global training, service, and support. Technical support scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging 8 years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Applied Biosystems™ RapidINTEL™ Sample Cartridge Evaluation Kit (Applied Biosystems™)

The Applied Biosystems RapidINTEL Sample Cartridge, 10-Pack Evaluation Kit, provides 10 self-contained, easy-to-use sample cartridges that contain all necessary reagents for sample preparation, including GlobalFiler Express chemistry. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and receive the lab-quality forensic DNA profile information within two hours.

Notes:
1. For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).
2. Use of this sample cartridge product requires software version 1.1 or higher on your Applied Biosystems RapidHIT ID System. Contact you local support representative for more information.

Features of the kit include:
• One-minute hands-on-time workflow provided by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 10 RapidINTEL sample cartridges

VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (for buccal swabs) (Applied Biosystems™)

The VeriFiler Express PCR Amplification Kit with Prep-n-Go Buffer (for buccal swabs) combines the features of our VeriFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer. The VeriFiler Express kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. It enables multiplex amplification of the 22 STR loci included in the GlobalFiler Express kit as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select, Identifiler, and VeriFiler Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector and other non-FTA substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features of the AmpFLSTR Identifiler Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample typeSuccess Rate
(50 rfu Threshold)
Success Rate
(150 rfu Threshold)
Number of Samples Tested
Blood on FTA Card99.4-100%
Mean: 99.5%
95.7-98.8%
Mean: 97.3%
414
Buccal on FTA Card91.8-99.4%
Mean: 97.1%
84.2-95.5%
Mean: 90.9%
643
Buccal on Bode Buccal
DNA Collector Card
97.5-98.8%
Mean: 97.8%
88.1-98.8%
Mean: 94.0%
582


Table 1: As part of its rigorous development, the Identifiler Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA Cards and Buccal DNA Collectors were amplified with the Identifiler Direct Kit, and the resulting data was analyzed using GeneMapper ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler Direct Kit is part of a new generation of AmpFLSTR Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR STR Kits, instrumentation, software, and consumables.

The Identifiler Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler Direct Kit should perform its own internal validation studies.

The Identifiler Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

Quantifiler™ HP DNA Quantification Kit (Applied Biosystems™)

The Quantifiler HP (Human Plus) DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal or miniSTR) and streamlines the workflow while increasing downstream analysis success rates. The kit includes enhanced quantity and quality indicators that help deliver accurate, sensitive, and specific results that work together with the HID Real Time PCR Analysis Software v1.2 to enable informative and integrated workflow decisions. The Quantifiler HP kit and the HID v1.2 software are designed for use with the Applied Biosystems 7500 Real-Time PCR System.

Highly Sensitive
• <1 pg/µL limit of detection. Quantifies DNA concentrations from 0.005 ng/uL to >50 ng/uL, enabling the use of a broad range of concentrations, with flexible protocols for higher concentration samples.

Highly Specific
• Detects human and higher primate DNA

Highly Informative
• Quantifies DNA from a wide variety of sources, including buccal swabs, blood, semen, tissue, and other human DNA sources, making this kit suitable for casework sample screening, databasing, and paternity labs
• Guides selection of the optimal AmpFLSTR PCR Amplification Kit (autosomal or miniSTR)
• Quality Index helps improve prediction of STR amplification performance and estimation of DNA quantity in degraded samples, especially in the presence of inhibitors.
• Enhanced inhibition detects the presence of PCR inhibitors (suggesting additional purification of extract and/or whether a mini STR kit like the MiniFiler, NGM SElect, or GlobalFiler kit may be necessary).

Highly Reliable
• Uses gold-standard TaqMan real-time PCR technology already widely adopted in forensic laboratories. Real-time PCR has the widest dynamic range of any DNA detection technology allowing for more accurate quantification of low level samples.

Highly Efficient
• The quantification system minimizes sample consumption and streamlines workflow, with results in ~1 hour.
• Optimized and validated as part of a complete DNA testing system with the Applied Biosystems 7500 Real-Time PCR System, AmpFLSTR PCR Amplification kits and HID Real Time PCR Analysis Software v 1.2 to enable high quality STR profiling results.

A Highly Informative Workflow Tool
The Quantifiler HP DNA Quantification Kit has been optimized and validated as part of a complete DNA testing system with the AmpFLSTR PCR Amplification kits. The chemistry is compatible with commonly used extraction technologies for easy integration into current lab workflows.This enables the Quantifiler HP kit to function as a critical component of an improved and integrated workflow for more effective processing of forensic casework samples. Both the kit and the software are fully automatable with most liquid handling robotic platforms.

Comparison to Quantifiler Human Kits
There are several specific differences between the Quantifiler HP DNA Quantification Kit and the Quantifiler Human DNA Quantification Kit (Cat. No. 4343895), including:
Target Loci: The Quantifiler HP kit uses multiple-copy target loci for improved detection sensitivity. There are two human-specific target loci: Small Autosomal and Large Autosomal. Each consists of multiple copies dispersed on various autosomal chromosomes.
Optimized Chemistry: The assay chemistry has been optimized for more efficient multiplexing, faster PCR cycle
times (~1 hour), and better inhibitor tolerance. The overall performance improvements allow the Quantifiler HP kit to better match the enhanced performance of newer STR kits that are designed to accommodate more challenging samples, like the Identifiler Plus, NGM SElect, or GlobalFiler PCR Amplification kits.
DNA Standard: The Quantifiler HP DNA Standard is pooled, human genomic, smaller molecular weight DNA for improved stability, homogeneity, and correlation with forensic samples. There is a new five-point standard curve protocol that helps improve efficiency with more samples on a plate.
Packaging Features: To help laboratories control for and minimize the potential of reagent contamination, as well as to better accommodate automated platforms, the Quantifiler HP DNA Quantification Kit has some key packaging features:
    --Four 100-reaction individual-use screw cap tubes (1 tube will process a 96 well plate)
    --The DNA standard is packaged in a separate box.
    --Both the reagent and DNA standard box are shipped together in an STR-style sleeve with new peel & stick reagent lot barcode labels. This allows customers to separate out the reagents easily for special storage upon arrival at the laboratory.

AmpFLSTR™ Huaxia™ PCR Direct Amplification Kit (Applied Biosystems™)

The AmpFLSTR Huaxia PCR Direct Amplification Kit employs a validated "direct" amplification chemistry designed specifically for the China forensic community to use with DNA database samples and other single source DNA samples. The Huaxia PCR Direct Amplification kit amplifies the discriminating loci set in the Applied Biosystems Sinofiler Kit, while enabling high quality direct PCR amplification of reference samples without the need for incubation, extraction, or purification.

The Huaxia PCR Direct Amplification kit:

• Facilitates high throughput and automated processing
• Accelerates time-to-result while maintaining high profile quality
• Eliminates extraction/purification costs and reduces labor and consumable costs
• Reduces the number of pre-amplification processing steps, minimizing the possibility of contamination or other procedural errors
• Uses the same loci and primer sequences as the Sinofiler kit, which:
     -- Offers high discrimination for Chinese populations
     --Maximizes concordance and facilitates comparison with existing data/database
     --Streamlines validation and implementation

Direct Amplification While Minimizing PCR Inhibition
By overcoming PCR inhibition and enhancing amplification of all the Sinofiler loci, the Huaxia PCR Direct Amplification kit delivers direct amplification capability to the Sinofiler primer set, allowing database laboratories to eliminate work steps and reduce time and labor required to generate STR profiles. In addition, the Huaxia kit allows for amplification in less time than is currently required.

Higher First-pass Success Rates Improve Productivity
The Huaxia PCR Direct Amplification kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:

• Well-resolved peaks above the background 'noise' level
• Minimal occurrence of allelic drop-out or off-scale data
• Clean baselines with fewer artifacts
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

With the Huaxia kit, there is no need for sample extraction, purification, or quantification, enabling a substantial reduction in labor and consumable costs compared to the standard PCR amplification workflow.

Simply "Punch and Go"
The kit includes primer mix, reaction mix, positive control DNA, and allelic ladder. The enzyme is contained within the reaction mix, so the Huaxia kit contains fewer tubes and fewer boxes than the Sinofiler kit. Simply punch a 1.2 mm disc into each well of a 96-well plate, add the master mix, and go to thermocycling. No washing, no extracting, and no quantifying. It's as simple as punch and go.

Note: For Forensic or Paternity Use Only.

IMPORTANT NOTICE

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

AmpFLSTR™ NGM™ PCR Amplification Kit (Applied Biosystems™)

The NGM Kit is a highly robust Short Tandem Repeat (STR) multiplex kit which utilizes a single protocol to amplify the 10 SGM Plus loci (D2S1338, D3S1358, D8S1179, D16S539, D18S51, D19S433, D21S11, FGA, TH01, vWA, Amelogenin) together with the additional loci approved by the European Union Council for the expansion of the European Standard Set (D10S1248, D22S1045, D2S441, D1S1656 & D12S391). The kit combines the latest advances in buffer optimization, primer synthesis and thermal cycling optimization to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM Kit:

• Simultaneous amplification of the SGM Plus kit loci plus 5 additional ENFSI/EDNAP recommended loci to provide powerful discrimination and facilitate cross-border data sharing
• Unprecedented performance in the presence of significant inhibitors combined with enhanced profile and locus balance
• Increased ability to recover information from degraded samples through a concentration of highly discriminatory loci in the low molecular weight region
• Greater sensitivity for improved amplification of low-level samples and better detection of the minor contributor in mixed samples
• Exceptional profile quality and clean baseline resulting from improved primer synthesis and purification processes
• 100% concordance with previously typed samples through maintenance of all STR primer sequences for loci common to the SGM Plus, Identifiler and SEfiler Plus Kits
• Proprietary and proven mobility modifier technology enables optimal locus spacing
• One kit, one protocol delivers high quality results from both database and casework samples
• Streamlined workflow with reduced amplification time, enzyme pre-formulated within the master mix and a larger package size option (1000 reactions) for high-throughput laboratories
• Optimized as part of an integrated reagent, instrumentation and software system for maximum performance and efficiency
• Outstanding discrimination and robustness offers utility for specialized applications including complex paternity/kinship, disaster victim identification and missing persons cases

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

VeriFiler™ Plus PCR Amplification Kit (Applied Biosystems™)

The Applied Biosystems VeriFiler Plus PCR Amplification Kit is a 6-dye, 25-locus STR kit that amplifies all of the loci present in the Chinese National autosomal DNA database, as well as all required CODIS markers. The VeriFiler Plus kit was specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples. The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, including the highly discriminatory Penta D and Penta E loci. The VeriFiler Plus kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. As with all Applied Biosystems STR kits, the VeriFiler Plus kit is ISO18385 Forensic DNA Grade, giving you piece of mind when using this kit for your casework samples.

Key features of the VeriFiler Plus PCR Amplification Kit include:
• Amplification of all Chinese National database loci and CODIS-required markers
• Concordance with Huaxia Platinum and VeriFiler Express kits; the VeriFiler Plus kit contains the same loci as these databasing kits
• High sensitivity enabled by a high-volume sample load (up to 17.5 µL)
• 11 mini-STRs maximize information recovery from degraded DNA samples
• Internal Quality Control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation, providing actionable information regarding further sample processing or testing
• Very high discrimination power provided by the 25 loci
• Gender confirmation through two Y-chromosome markers
• Efficient workflow with 76-minute PCR cycle time

Casework kit with loci matching DNA profiles in databases
The VeriFiler Plus PCR Amplification Kit was developed to offer the highest level of compatibility between DNA databases and the casework samples genotyped with this kit. Specifically, the VeriFiler Plus kit amplifies all of the loci that are included in the DNA profiles used to build important national and local databases, including the 20 core loci proposed by the Chinese Ministry of Public Security. All required CODIS markers and the highly discriminatory Penta D and Penta E markers are included as well. With 25 markers, the VeriFiler Plus kit offers extremely high discrimination power, enabling matching of casework samples with database profiles with the highest level of confidence.

Exceptional sensitivity enables recovery of more alleles
Developed specifically for casework applications, the VeriFiler Plus PCR Amplification Kit provides DNA analysts with the capability to recover more information from casework samples. The higher sample load volume (up to 17.5 µL) and the inclusion of 11 mini-STRs, as defined by amplicons less than 250 base pairs, provide the highest chance of allele detection. As crime laboratories move toward using DNA forensics in volume crimes, detection of profiles or even partial profiles from samples with a low amount of DNA becomes increasingly important. With an optimized buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples, the VeriFiler Plus kit delivers the capability to provide maximum possible information even from the most challenging samples.

IQC system for more informative results and better decision guidance
The VeriFiler Plus PCR Amplification Kit includes an IQC system that consists of two exogenous, synthetic DNA sequences with primers specific for each IQC target that help distinguish DNA sample degradation from amplification reaction inhibition. In addition, the IQC system serves as a positive control for PCR amplification. The IQC system results, together with an assessment of a DNA profile’s electropherogram, provide DNA analysts with confirmation of inhibition or inferred degradation by virtue of a lack of inhibition, providing data needed to make decisions on additional steps that can be taken to extract more information from a sample.

Experience matters
Thermo Fisher Scientific is the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging eight years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

Applied Biosystems™ RapidHIT™ ID ACE GlobalFiler™ Express 50 Sample Kit (Applied Biosystems™)

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

The Applied Biosystems RapidHIT ID ACE GlobalFiler Express 50 Sample Kit provides 50 self-contained, easy-to-use sample cartridges that contain all necessary reagents for sample preparation, including GlobalFiler Express chemisty, for sample preparation. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and in 90 minutes receive the lab-quality forensic DNA profile.

Features of the kit include:
• 1-minute hands-on-time workflow provided by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 50 RapidHIT ID ACE GlobalFiler Express sample cartridges
• 2 positive control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 2 negative control RapidHIT ID ACE GlobalFiler Express sample cartridges

Applied Biosystems™ RapidINTEL™ Sample Cartridge Kit (Applied Biosystems™)

The Applied Biosystems RapidINTEL Sample Cartridge, 50-Pack Kit, provides 50 self-contained, easy-to-use sample cartridges that contain all necessary reagents for sample preparation, including GlobalFiler Express chemistry. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and receive the lab-quality forensic DNA profile information within two hours.

Notes:
1. For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).
2. Use of this sample cartridge product requires software version 1.1 or higher on your Applied Biosystems RapidHIT ID System. Contact you local support representative for more information.

Features of the kit include:
• One-minute hands-on-time workflow provided by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 50 RapidINTEL sample cartridges
• 2 positive-control ACE GlobalFiler Express sample cartridges
• 2 negative-control ACE GlobalFiler Express sample cartridges

GlobalFiler™ PCR Amplification Kit (Applied Biosystems™)

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples; for database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

Key Features:

• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

Quantifiler™ Human DNA Quantification Kit (Applied Biosystems™)

This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors.

• Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs.
• Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow.
• Pre-formulated Internal PCR Control (IPC) included in each reaction allows for quick monitoring of amplification success and identifies samples that may contain inhibitors.
• Real-time PCR has the widest dynamic range of any DNA detection technology allowing for more accurate quantification of low level samples.
• Quantifies a broad range of DNA concentrations from 0.023 ng⁄uL to>50 ng⁄uL enabling the use of a broad range of DNA concentrations.

Minimize repeat STR analysis
This kit easily integrates into a short tandem repeat (STR) analysis workflow. It provides a more accurate prediction of the amount of DNA input required for optimal STR amplification.

Easy to use, Single Tube Format
PCR reaction and quantification performed in single tube helping to minimize contamination.

Trusted TaqMan Technology
TaqMan based assays are used throughout the scientific community for scientific discovery assays including: gene expression, DNA, presence or absence of a nucleic acid, and SNP genotyping. The Quantifiler kits utilize this widely known and trusted technology for the quantification of the total male DNA (see figure 1).

Experience Matters
When you partner with Applied Biosystems, you gain access to the extensive knowledge and experience of our forensic support team and training resources. Our HID University provides continuing education for DNA professionals, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technication assistance with instrumentation.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs (Applied Biosystems™)

The AmpFLSTR Identifiler Direct PCR Amplification Kit & Prep-n-Go Buffer (for Buccal Swabs) combines the features of our AmpFLSTR Identifiler Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for Buccal Swabs). The Identifiler Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key Features:

• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler kit, which:

• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.