Forensic Testing Kits

Quantifiler™ Human DNA Quantification Kit (Applied Biosystems™)

This real-time PCR-based kit is designed for the quantification of amplifiable human DNA that is present in a sample, and has been validated for human identification applications. An internal PCR control is formulated into each reaction to identify samples that may contain PCR inhibitors.

• Quantifies DNA from a wide variety of sources including buccal swabs, blood, semen, tissue, and other human DNA sources making this kit suitable for casework, databasing, and paternity labs.
• Chemistry compatible with commonly used extraction technologies for easy integration into current lab workflow.
• Pre-formulated Internal PCR Control (IPC) included in each reaction allows for quick monitoring of amplification success and identifies samples that may contain inhibitors.
• Real-time PCR has the widest dynamic range of any DNA detection technology allowing for more accurate quantification of low level samples.
• Quantifies a broad range of DNA concentrations from 0.023 ng⁄uL to>50 ng⁄uL enabling the use of a broad range of DNA concentrations.

Minimize repeat STR analysis
This kit easily integrates into a short tandem repeat (STR) analysis workflow. It provides a more accurate prediction of the amount of DNA input required for optimal STR amplification.

Easy to use, Single Tube Format
PCR reaction and quantification performed in single tube helping to minimize contamination.

Trusted TaqMan® Technology
TaqMan® based assays are used throughout the scientific community for scientific discovery assays including: gene expression, DNA, presence or absence of a nucleic acid, and SNP genotyping. The Quantifiler® kits utilize this widely known and trusted technology for the quantification of the total male DNA (see figure 1).

Experience Matters™
When you partner with Applied Biosystems®, you gain access to the extensive knowledge and experience of our forensic support team and training resources. Our HID University provides continuing education for DNA professionals, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technication assistance with instrumentation.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® Identifiler® Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler® Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA® Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other non-FTA® substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems® Prep-n-Go™ Buffer.

Key Features of the AmpFLSTR® Identifiler® Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler® kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample typeSuccess Rate
(50 rfu Threshold)
Success Rate
(150 rfu Threshold)
Number of Samples Tested
Blood on FTA™ Card99.4-100%
Mean: 99.5%
95.7-98.8%
Mean: 97.3%
414
Buccal on FTA™ Card91.8-99.4%
Mean: 97.1%
84.2-95.5%
Mean: 90.9%
643
Buccal on Bode Buccal
DNA Collector™ Card
97.5-98.8%
Mean: 97.8%
88.1-98.8%
Mean: 94.0%
582


Table 1: As part of its rigorous development, the Identifiler® Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA® Cards and Buccal DNA Collectors™ were amplified with the Identifiler® Direct Kit, and the resulting data was analyzed using GeneMapper® ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler® Direct Kit is part of a new generation of AmpFLSTR® Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler® Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.

The Identifiler® Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler® Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler® Direct Kit should perform its own internal validation studies.

The Identifiler® Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Applied Biosystems™)

Optimized for casework, the AmpFLSTR® Identifiler® Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler® Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

Features of the AmpFLSTR® Identifiler® Plus PCR Amplification Kit include:

• Amplifies the same loci as the Identifiler® kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler® kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler™ Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® and SGM Plus® kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts

Other Improvements and Benefits
The improvements in the Identifiler® Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler® kit. In addition, the Identifiler® Plus Master Mix includes AmpliTaq Gold® DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.

None of the formulation enhancements included in the Identifiler® Plus kit have required modifications to the Identifiler® Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler™ kit has been designed to work in conjunction with the Identifiler® Plus kit, utilizing miniSTR technology to help maximize recovery of information.

The Identifiler® Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® or SGM Plus® kits, and facilitate comparison with existing DNA databases.

The Confidence to Do More
The Identifiler® Plus kit is part of a new generation of AmpFLSTR® kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler® Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.

Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.

VeriFiler™ Express PCR Amplification Kit (Applied Biosystems™)

The VeriFiler™ Express PCR Amplification Kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. The VeriFiler Express kit enables multiplex amplification of the 22 STR loci included in the GlobalFiler™ Express kit, as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs (Applied Biosystems™)

The AmpFLSTR® Identifiler® Direct PCR Amplification Kit & Prep-n-Go™ Buffer (for Buccal Swabs) combines the features of our AmpFLSTR® Identifiler® Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer (for Buccal Swabs). The Identifiler® Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler® kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go™ Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key Features:

• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler® kit, which:

• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

Quantifiler™ Duo DNA Quantification Kit (Applied Biosystems™)

The Quantifiler® Duo DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal STR chemistry (autosomal, Y-STR or miniSTR) and streamlines the workflow while increasing downstream analysis success rates.

• Highly Accurate: determines the ratio of human male to female DNA, even with minute male and excess female DNA (1:1000 or greater).
• Highly Informative:
•Guides selection of the optimal AmpFLSTR® PCR Amplification Kit (autosomal, Y-STR or miniSTR),
•Improves prediction of STR amplification performance and estimation of DNA quantity in degraded samples,
•Detects the presence of PCR inhibitors (suggesting additional purification of extract and⁄or MiniFiler™ kit analysis may be necessary).
• Highly Sensitive: 6 pg⁄µl limit of detection.
• Highly Specific: for human and higher primate DNA.
• Highly Efficient: dual quantification system minimizes sample consumption and streamlines workflow.
• Highly Reliable: uses the gold standard TaqMan® real-time PCR technology already widely adopted in forensic laboratories.
• Highly Integrated: optimized and validated as part of a complete DNA testing system with the Applied Biosystems® 7500 Real-Time PCR System and AmpFLSTR® PCR Amplification Kits to provide the highest quality STR profiling results.

A Highly Informative Tool
The Quantifiler® Duo kit has been optimized and validated as part of a complete DNA testing system with the AmpFLSTR® PCR Amplification Kits.
This enables it to function as a critical component of an improved and integrated workflow for more effective processing of forensic casework samples. The workflow diagram in Figure 1 illustrates how the kit functions as a “sample assessment system" which can efficiently detect the presence of inhibitors, determine the ratio of male to female DNA, and guide selection of the optimal AmpFLSTR® PCR Amplification Kit (autosomal, Y-STR or miniSTR) to maximize recovery of interpretable STR profiles.

Kit Configuration
This kit uses increased amplicon sizes for improved prediction of STR amplification performance and estimation of DNA quantity in degraded samples. The amplicon sizes are similar for all three targets to avoid preferential PCR. The kit uses single copy targets for superior accuracy and consistency in quantification results (multi-copy targets are affected by variability between individuals and decrease correlation with the single copy targets in STR kits).

Comparison to Quantifiler® Human and Y Kits
There are many specific differences with the current Quantifiler® kits including:

• The Quantifiler® Duo kit uses an alternative human DNA target (RPPH1) and increased amplicon sizes to improve prediction of STR amplification performance and estimation of DNA quantity in degraded samples.
• The Quantifiler® Duo DNA Standard is pooled human male genomic DNA (rather than cell-line DNA) for improved stability and correlation with forensic samples.
• The Quantifiler® Duo PCR Reaction Mix has been optimized for expanded multiplex performance.
• The Quantifiler® Duo kit has been designed to guide selection of appropriate AmpFLSTR® Kit chemistry and maximize recovery of interpretable STR profiles.

AmpFLSTR™ NGM™ PCR Amplification Kit (Applied Biosystems™)

The NGM™ Kit is a highly robust Short Tandem Repeat (STR) multiplex kit which utilizes a single protocol to amplify the 10 SGM Plus™ loci (D2S1338, D3S1358, D8S1179, D16S539, D18S51, D19S433, D21S11, FGA, TH01, vWA, Amelogenin) together with the additional loci approved by the European Union Council for the expansion of the European Standard Set (D10S1248, D22S1045, D2S441, D1S1656 & D12S391). The kit combines the latest advances in buffer optimization, primer synthesis and thermal cycling optimization to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM™ Kit:

• Simultaneous amplification of the SGM Plus® kit loci plus 5 additional ENFSI/EDNAP recommended loci to provide powerful discrimination and facilitate cross-border data sharing
• Unprecedented performance in the presence of significant inhibitors combined with enhanced profile and locus balance
• Increased ability to recover information from degraded samples through a concentration of highly discriminatory loci in the low molecular weight region
• Greater sensitivity for improved amplification of low-level samples and better detection of the minor contributor in mixed samples
• Exceptional profile quality and clean baseline resulting from improved primer synthesis and purification processes
• 100% concordance with previously typed samples through maintenance of all STR primer sequences for loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ Kits
• Proprietary and proven mobility modifier technology enables optimal locus spacing
• One kit, one protocol delivers high quality results from both database and casework samples
• Streamlined workflow with reduced amplification time, enzyme pre-formulated within the master mix and a larger package size option (1000 reactions) for high-throughput laboratories
• Optimized as part of an integrated reagent, instrumentation and software system for maximum performance and efficiency
• Outstanding discrimination and robustness offers utility for specialized applications including complex paternity/kinship, disaster victim identification and missing persons cases

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

NGM Detect™ PCR Amplification Kit (Applied Biosystems™)

The Applied Biosystems NGM Detect PCR Amplification Kit is the first 6-dye STR kit designed specifically for those laboratories using the European Standard Set (ESS) of loci. The kit offers excellent sensitivity and provides an alternate marker configuration to the well-established NGM SElect PCR Amplification Kit format to maximize information recovery, even from degraded casework samples.

As with all Applied Biosystems STR kits, the NGM Detect kit is ISO18385 Forensic DNA Grade, giving you piece of mind when choosing this kit for your casework samples.

The key features of NGM Detect kit are:
Increased database searches by virtue of prioritization of markers with the highest representation in historic databases
Optimized positioning of SE33 in the sub-350 bp range to support mixture analysis
Dual amplification compatibility when partnered with the NGM SElect kit
High sensitivity through a new master mix formulation coupled with the ability to add up to 15 μL of sample to the reaction
Fast PCR cycle time—<60 min
Additional Y marker enabling gender confirmation
Integrated Quality Control System to verify reaction set up and assess the presence of inhibition in the sample

Increased database searches
The European Standard Set (ESS) of loci was established to enable cross-border information sharing and was composed of seven SGM/SEFiler Plus markers. The ESS was expanded to 12 loci in 2009 to include more markers to enable wider compatibility between neighboring countries.

Because of this, a large number of historic crime-scene profiles contained in databases consist of SGM/SEFiler Plus marker data. When dealing with challenging casework, such as cases involving degraded samples, the information recovery is limited. In this situation, recovering the SGM/SEFiler Plus markers is key for producing a partial DNA profile that has maximum overlap with older data contained in databases, giving a higher probability of a match and providing valuable investigative leads.

The NGM Detect kit takes full advantage of the space afforded by 6 dyes and features optimized marker positioning, placing the SGM/SEFiler Plus markers in the low molecular weight (LMW) range to help ensure the highest compatibility with historic datasets and the possibility of a higher chance of generating investigative leads.

Optimized positioning of SE33
The highly polymorphic SE33 marker is useful for mixture analysis but extends up to 500 bp in most STR kits. As a result, it is one of the first markers to drop out in degraded samples. In the NGM Detect kit, SE33 extends to a maximum of 351 bp to help to significantly increase the chances of recovery of this highly informative marker in challenging samples.

Dual amplification compatibility
Dual amplification, where two different STR kits are used to amplify a sample, is a strategy that can be used to confirm or extend a DNA profile result for increased confidence in the final result in the former and to obtain additional information in the latter.The NGM Detect kit is an advanced partner for the NGM SElect kit, featuring the same loci, but with different primer sequences and different marker placement. When using the two kits in combination, different primer sequences provide confidence that the consensus profile generated reflects the ‘true’ genotype of the sample. The complimentary marker positioning places mini-STR loci in the NGM Detect kit in the high molecular weight (HMW) range in the NGM Select kit and vice versa, meaning that additional alleles can be recovered from degraded samples when using the two kits in combination. Together the NGM Detect and NGM Select system delivers 12 independent mini-STRs.

Integrated Quality Control System
The IQC assay consists of two synthetic sequences not found in nature with primers specific for each of the targets. It provides positive confirmation that the assay is functioning as expected in a given run.

This system is particularly useful to confirm the validity of negative results and can also be used to distinguish samples that are degraded versus those that contain PCR inhibitors, supplementing data analysis and informing downstream processing decisions.

Experience matters
The NGM Detect kit is part of a fully integrated and validated forensic DNA workflow backed by superior global training, service, and support. Technical support scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging 8 years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ Express PCR Amplification Kit (Applied Biosystems™)

The GlobalFiler® Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler™ Express Kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler® PCR Amplification Kit.

Key Features:

• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler® Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.

GlobalFiler™ Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs) (Applied Biosystems™)

The GlobalFiler® Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs) combines the features of our GlobalFiler® Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer (for buccal swabs). The GlobalFiler® Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go™ Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key features:
• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput and automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time-to-result with minimal reduction in data quality
• Minimizes extraction and purification costs and helps reduce the cost of labor and consumables

In addition, the GlobalFiler® Express PCR Amplification Kit combines maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency.

The GlobalFiler® Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler® PCR Amplification Kit.

AmpFLSTR™ Yfiler™ Direct Kit (Applied Biosystems™)

The AmpFLSTR® Yfiler® Direct Kit enables forensic laboratories to perform high-quality Y-STR analysis of single-source reference samples using direct PCR amplification. This kit amplifies the same 17 Y-STR loci as the AmpFLSTR® Yfiler® Kit and is designed to generate accurate results with faster thermocycling conditions than the Yfiler® Kit. Blood or buccal samples on treated paper (FTA® Card or NUCLEIC-CARD™ System) can be punched into PCR plates or tubes and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other untreated substrates can be processed without additional workflow steps using the Applied Biosystems® Prep-n-Go™ Buffer.

Key Features:

• Supports direct PCR amplification (no DNA extraction or quantification required) of single source reference samples on treated and untreated paper
• Provides maximum concordance with the Yfiler® kit
• Designed to deliver faster cycling times through the introduction of a new fast-capable enzyme (~2.5 hours)

In addition, the Yfiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Yfiler® kit, which:

• Facilitates comparison with casework data
• Streamlines validation and implementation

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.

The AmpFLSTR® Yfiler® Direct Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification.

The Yfiler® Direct kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.

IMPORTANT NOTICE:
The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

AmpFLSTR™ NGM SElect™ PCR Amplification Kit (Applied Biosystems™)

The NGM SElect™ Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect™ Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization. This enables the kit to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM SElect™ Kit:

• Exceptional data quality and discrimination capabilities support international data sharing
• Enables significantly enhanced performance on inhibited and degraded samples
• Enables greater sensitivity
• Maximizes concordance with previously typed samples
• Facilitates confident interpretation, promotes an optimized workflow and streamlines the implementation process

Data Quality Worth Sharing
The NGM SElect™ Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP), and includes the expanded European Standard Set of Loci (ESSL).

The NGM SElect™ kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel (Figure 1).

Configured for Success
Utilizing our expertise in 5-dye and mobility modifier technology, 17 loci are accommodated in a single amplification while maintaining optimum spacing (Figure 2). This permits unambiguous allele designation. All amplicons fall below 450 base pairs (bp) to maximize performance across a wide range of forensic samples. The majority of loci are concentrated below 200 bp, enabling recovery of a greater number of alleles from challenging degraded samples.

Powerful Discrimination
The combination of loci in the NGM SElect™ kit generates the highest power of discrimination of any AmpFLSTR® kit (Table 1). This helps minimize adventitious matches when comparing large data sets (e.g. for cross border data exchange). The NGM SElect™ kit also offers utility for other applications requiring higher levels of discrimination, including:
• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Permits Significantly Enhanced Performance on Inhibited and Degraded Samples
Leveraging the recent advances in PCR amplification technology pioneered with the NGM™ kit, the NGM SElect™ kit enables recovery of data from severely inhibited and degraded samples. For example, the NGM SElect™ kit is able to extract full profiles even from samples containing high concentrations of PCR inhibitors such as humic acid (Figure 3).

Performs with Greater Sensitivity
The NGM SElect™ Kit benefits from the same sensitivity enhancements as the NGM™ Kit, resulting in superior allele recovery at lower input levels (Figure 4). This can also enhance detection of the minor contributor in a mixed sample. The standard 29 cycle, 1 ng DNA input protocol enables maximum profile balance, and a validated 30 cycle protocol is also available. The higher cycle number leads to a corresponding increase in signal, increasing the possibility of allele detection from lower input DNA amounts. The use of 30 cycles also results in offscale data at an input of 1 ng (Figure 4); therefore, laboratories wishing to adopt this protocol should determine the optimum input DNA appropriate to their workflow through internal validation studies.

Maximizes Concordance
The NGM SElect™ Kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit as much as possible.

Facilitates Confident Data Interpretation
The NGM SElect™ kit benefits from improvements in primer synthesis techniques resulting in significantly cleaner baselines than previous AmpFLSTR® kits (Figure 5). The lack of dye related artifacts, even at 30 cycles, improves the ability of the analyst to interpret data, particularly for low level samples. Confident data interpretation is further enhanced by other improvements in profile quality, including:
• Enhanced sensitivity and robustness
• Maximized heterozygote and intracolor balance
• Optimum dye set utilization

These features also help facilitate streamlined processing of single source samples by expert system software such as GeneMapper® ID-X.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ kit has been designed with ease of implementation and use in mind. This is a single kit solution that delivers high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs.

The kit workflow is very similar to other AmpFLSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened by approximately one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

AmpFLSTR™ Yfiler™ PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® Yfiler® PCR Amplification Kit permits the analysis of complex male:female DNA mixtures that often yield ambiguous or inconclusive results with autosomal STRs.

Key Features of the AmpFLSTR® Yfiler® PCR Amplification Kit:

• Analyze trace quantities of male DNA that may be present in a large background of female DNA
• Combine the inherent male specificity of Y-STRs with the robust performance of an AmpFLSTR® kit
• Amplify 17 Y-STR loci in a single PCR reaction which yields the highest discriminatory capacity of any commercially available Y-STR kit
• Search the user-friendly on-line haplotype database to easily determine the frequency of any Yfiler® kit haplotype
• Use this powerful tool for the analysis of cases that contain a suspect(s)

Combines the Inherent Male Specificity of Y-STRs with the Robust Performance of an AmpFLSTR® Kit
Mixtures of body fluids and/or tissues from a male and female are frequently encountered with sexual assault casework and other violent crimes. These mixed samples often contain trace quantities of male DNA present in a large background of female DNA. Typical samples can include vaginal swabs containing a small number of sperm cells, body swabs from female victims of oral assaults, and fingernail scrapings/clippings from female victims of violent assaults. Standard autosomal STR analysis may be unable to detect the minor male contributor in these types of samples, while the Yfiler® kit can routinely detect a minor male contributor present at less than 1:1000.

Provides the Highest Discriminatory Capacity of Any Commercially Available Y-STR Kit
The AmpFLSTR® Yfiler® PCR Amplification Kit amplifies 17 Y-STR loci in a single PCR reaction using 5-dye chemistry to provide the highest discriminatory capacity of any commercially available Y-STR kit. The Yfiler® kit can distinguish more than 98% of all unrelated males from one another, and therefore offers the discriminatory power necessary to conduct meaningful forensic DNA analysis.

A Powerful Tool for Suspect Casework
The Yfiler® kit is a powerful tool for the analysis of cases that contain a suspect(s). A significant proportion of DNA cases contain a suspect who has been identified by conventional law enforcement techniques. For these cases, the Yfiler® kit is a powerful tool that can be used to include or exclude a suspect(s) associated with an investigation.

Complete Validated Solution
Applied Biosystems® offers a complete solution for your Human Identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® kits, instrumentation, software, and consumables. The Yfiler® kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

Note: For Forensic or Paternity Use Only.

Important Notice

AmpFLSTR™ Profiler Plus™ PCR Amplification Kit (Applied Biosystems™)

We have combined the advantages of PCR-based testing and fluorescence detection into a highly discriminating kit designed for human identification applications. The AmpFLSTR® Profiler Plus® PCR Amplification Kit amplifies nine tetranucleotide short tandem repeat (STR) loci and the Amelogenin locus in a single reaction tube. The STR loci amplified are D3S1358, D5S818, D7S820, D8S1179, D13S317, D18S51, D21S11, FGA, and vWA.

Key Features of the AmpFLSTR® Profiler Plus® PCR Amplification Kit:

• Nine tetranucleotide STR loci and the Amelogenin locus co-amplified in a single tube for ease of use
• Use in conjunction with COfiler PCR Amplification Kit
• Includes AmpliTaq Gold® DNA Polymerase for increased sensitivity and specificity
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing

Clear Differentiation
The Amelogenin locus is used for gender identification because products of different lengths are generated from the X and Y chromosomes.

Quality Results
The AmpFLSTR® Profiler Plus® reagents and protocols produce the quality results necessary for human identification applications. To increase the success of obtaining results in the presence of enzyme inhibitors, bovine serum albumin (BSA) has been included in the AmpFLSTR® PCR Reaction Mix.

Sensitivity
Thermal cycler times and temperatures selectively amplify DNA sequences while providing the necessary degree of sensitivity. The recommended range of input DNA is 1.0 to 2.5 ng. Furthermore, instrument detection protocols yield reproducible results with excellent resolution and precision in sizing alleles.

Reliability
AmpFLSTR® Profiler Plus® kit is subjected to rigorous quality control testing to ensure reliable performance. Experiments are performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21-43). These results have been summarized and submitted for peer review publication.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) (Applied Biosystems™)

The VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) combines the features of our VeriFiler™ Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer. The VeriFiler Express kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. It enables multiplex amplification of the 22 STR loci included in the GlobalFiler™ Express kit as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.