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AmpFLSTR™ MiniFiler™ PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® MiniFiler™ PCR Amplification Kit increases your ability to obtain DNA results from compromised samples that previously would have yielded limited or no genetic data. This means cold cases can come off the shelf for re-analysis and new, challenging samples have a better chance of delivering interpretable results. This is the world's first commercially available miniSTR kit.

Features of the AmpFLSTR® MiniFiler™ PCR Amplification Kit:

• A single amplification provides information on the eight largest and most difficult to amplify loci in the AmpFLSTR® Identifiler® and SGM Plus®.
• With amplicon sizes less than 270 bp, MiniFiler™ is optimized for use with the most difficult types of samples including those that are degraded and contain inhibitors.
• Ability to overcome the inhibitory effects of substances such as heme and humic acid which are commonly encountered in forensic samples.
• Can be used as an adjunct to other commercially available amplification kits to recover the larger loci, which cannot be amplified or that drop out.
• Utilizes proven 5-dye technology allowing compatibility of workflows with Identifiler®.

Five Dye Technology Allows Enhanced Throughput
MiniFiler™ utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET™ and LIZ™ dyes were specifically designed to expand the spectral range of the 4 dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.

Experience Matters™
When you partner with us you gain access to the most extensive knowledge and experience of our forensic support team and training resources dedicated to human identification laboratories. Our HID University™ provides continuing education for DNA professional, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

Complete Validated Solution
We offer a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, and consumables. Minifiler is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

AmpFLSTR™ SGM Plus™ PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® SGM Plus® PCR Amplification Kit amplifies 10 STR loci and the gender marker Amelogenin yielding a highly informative kit for human identification, with a 1 in 5 X 1012 average probability of identity.

Key Features of the AmpFLSTR® SGM Plus® PCR Amplification Kit:

• Ten tetranucleotide STR loci and Amelogenin are co-amplified in a single PCR tube for ease of use and increased throughput
• Amplicon allele size ranges less than 360 base pairs ensure robust PCR amplification for all loci
• Conveys certain rights to perform research and forensic testing services when used with an authorized thermal cycler
• Conveys the PCR service rights for paternity testing

More Information
Six of the STR loci (D21, FGA, TH01, vWA, D8, D18) were developed and popularized by the Forensic Science Service (FSS) in the Second Generation Multiplex (SGM) system. To create the AmpFLSTR® SGM Plus® kit, a PCR multiplex was developed and optimized to included the six core SGM loci and Amelogenin, plus four additional STR loci (D3, D19, D16, D2). The AmpFLSTR® SGM Plus® kit significantly enhances the information obtained from a single PCR.

Reliable Results
The AmpFLSTR® SGM Plus® kit has been subjected to rigorous quality-control testing to ensure reliable performance. Experiments have been performed based upon the TWGDAM recommended guidelines (Technical Working Group on DNA Analysis Methods. 1995. Guidelines for a Quality Assurance Program for DNA Analysis. Crime Lab Digest 22:21 to 43).

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

Quantifiler™ HP DNA Quantification Kit (Applied Biosystems™)

The Quantifiler® HP (Human Plus) DNA Quantification Kit is a robust kit that enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal short tandem repeat (STR) analysis chemistry (autosomal or miniSTR) and streamlines the workflow while increasing downstream analysis success rates. The kit includes enhanced quantity and quality indicators that help deliver accurate, sensitive, and specific results that work together with the HID Real Time PCR Analysis Software v1.2 to enable informative and integrated workflow decisions. The Quantifiler® HP kit and the HID v1.2 software are designed for use with the Applied Biosystems® 7500 Real-Time PCR System.

Highly Sensitive
• <1 pg/µL limit of detection. Quantifies DNA concentrations from 0.005 ng/uL to >50 ng/uL, enabling the use of a broad range of concentrations, with flexible protocols for higher concentration samples.

Highly Specific
• Detects human and higher primate DNA

Highly Informative
• Quantifies DNA from a wide variety of sources, including buccal swabs, blood, semen, tissue, and other human DNA sources, making this kit suitable for casework sample screening, databasing, and paternity labs
• Guides selection of the optimal AmpFLSTR® PCR Amplification Kit (autosomal or miniSTR)
• Quality Index helps improve prediction of STR amplification performance and estimation of DNA quantity in degraded samples, especially in the presence of inhibitors.
• Enhanced inhibition detects the presence of PCR inhibitors (suggesting additional purification of extract and/or whether a mini STR kit like the MiniFiler™, NGM SElect™, or GlobalFiler™ kit may be necessary).

Highly Reliable
• Uses gold-standard TaqMan® real-time PCR technology already widely adopted in forensic laboratories. Real-time PCR has the widest dynamic range of any DNA detection technology allowing for more accurate quantification of low level samples.

Highly Efficient
• The quantification system minimizes sample consumption and streamlines workflow, with results in ~1 hour.
• Optimized and validated as part of a complete DNA testing system with the Applied Biosystems® 7500 Real-Time PCR System, AmpFLSTR® PCR Amplification kits and HID Real Time PCR Analysis Software v 1.2 to enable high quality STR profiling results.

A Highly Informative Workflow Tool
The Quantifiler® HP DNA Quantification Kit has been optimized and validated as part of a complete DNA testing system with the AmpFLSTR® PCR Amplification kits. The chemistry is compatible with commonly used extraction technologies for easy integration into current lab workflows.This enables the Quantifiler® HP kit to function as a critical component of an improved and integrated workflow for more effective processing of forensic casework samples. Both the kit and the software are fully automatable with most liquid handling robotic platforms.

Comparison to Quantifiler® Human Kits
There are several specific differences between the Quantifiler® HP DNA Quantification Kit and the Quantifiler® Human DNA Quantification Kit (Cat. No. 4343895), including:
Target Loci: The Quantifiler® HP kit uses multiple-copy target loci for improved detection sensitivity. There are two human-specific target loci: Small Autosomal and Large Autosomal. Each consists of multiple copies dispersed on various autosomal chromosomes.
Optimized Chemistry: The assay chemistry has been optimized for more efficient multiplexing, faster PCR cycle
times (~1 hour), and better inhibitor tolerance. The overall performance improvements allow the Quantifiler® HP kit to better match the enhanced performance of newer STR kits that are designed to accommodate more challenging samples, like the Identifiler® Plus, NGM SElect™, or GlobalFiler™ PCR Amplification kits.
DNA Standard: The Quantifiler® HP DNA Standard is pooled, human genomic, smaller molecular weight DNA for improved stability, homogeneity, and correlation with forensic samples. There is a new five-point standard curve protocol that helps improve efficiency with more samples on a plate.
Packaging Features: To help laboratories control for and minimize the potential of reagent contamination, as well as to better accommodate automated platforms, the Quantifiler® HP DNA Quantification Kit has some key packaging features:
    --Four 100-reaction individual-use screw cap tubes (1 tube will process a 96 well plate)
    --The DNA standard is packaged in a separate box.
    --Both the reagent and DNA standard box are shipped together in an STR-style sleeve with new peel & stick reagent lot barcode labels. This allows customers to separate out the reagents easily for special storage upon arrival at the laboratory.

AmpFLSTR™ NGM SElect™ PCR Amplification Kit (Applied Biosystems™)

The NGM SElect™ Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect™ Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization. This enables the kit to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM SElect™ Kit:

• Exceptional data quality and discrimination capabilities support international data sharing
• Enables significantly enhanced performance on inhibited and degraded samples
• Enables greater sensitivity
• Maximizes concordance with previously typed samples
• Facilitates confident interpretation, promotes an optimized workflow and streamlines the implementation process

Data Quality Worth Sharing
The NGM SElect™ Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP), and includes the expanded European Standard Set of Loci (ESSL).

The NGM SElect™ kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel (Figure 1).

Configured for Success
Utilizing our expertise in 5-dye and mobility modifier technology, 17 loci are accommodated in a single amplification while maintaining optimum spacing (Figure 2). This permits unambiguous allele designation. All amplicons fall below 450 base pairs (bp) to maximize performance across a wide range of forensic samples. The majority of loci are concentrated below 200 bp, enabling recovery of a greater number of alleles from challenging degraded samples.

Powerful Discrimination
The combination of loci in the NGM SElect™ kit generates the highest power of discrimination of any AmpFLSTR® kit (Table 1). This helps minimize adventitious matches when comparing large data sets (e.g. for cross border data exchange). The NGM SElect™ kit also offers utility for other applications requiring higher levels of discrimination, including:
• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Permits Significantly Enhanced Performance on Inhibited and Degraded Samples
Leveraging the recent advances in PCR amplification technology pioneered with the NGM™ kit, the NGM SElect™ kit enables recovery of data from severely inhibited and degraded samples. For example, the NGM SElect™ kit is able to extract full profiles even from samples containing high concentrations of PCR inhibitors such as humic acid (Figure 3).

Performs with Greater Sensitivity
The NGM SElect™ Kit benefits from the same sensitivity enhancements as the NGM™ Kit, resulting in superior allele recovery at lower input levels (Figure 4). This can also enhance detection of the minor contributor in a mixed sample. The standard 29 cycle, 1 ng DNA input protocol enables maximum profile balance, and a validated 30 cycle protocol is also available. The higher cycle number leads to a corresponding increase in signal, increasing the possibility of allele detection from lower input DNA amounts. The use of 30 cycles also results in offscale data at an input of 1 ng (Figure 4); therefore, laboratories wishing to adopt this protocol should determine the optimum input DNA appropriate to their workflow through internal validation studies.

Maximizes Concordance
The NGM SElect™ Kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit as much as possible.

Facilitates Confident Data Interpretation
The NGM SElect™ kit benefits from improvements in primer synthesis techniques resulting in significantly cleaner baselines than previous AmpFLSTR® kits (Figure 5). The lack of dye related artifacts, even at 30 cycles, improves the ability of the analyst to interpret data, particularly for low level samples. Confident data interpretation is further enhanced by other improvements in profile quality, including:
• Enhanced sensitivity and robustness
• Maximized heterozygote and intracolor balance
• Optimum dye set utilization

These features also help facilitate streamlined processing of single source samples by expert system software such as GeneMapper® ID-X.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ kit has been designed with ease of implementation and use in mind. This is a single kit solution that delivers high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs.

The kit workflow is very similar to other AmpFLSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened by approximately one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ Express PCR Amplification Kit (Applied Biosystems™)

The GlobalFiler® Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler™ Express Kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler® PCR Amplification Kit.

Key Features:

• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler® Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.

GlobalFiler™ PCR Amplification Kit (Applied Biosystems™)

The GlobalFiler® PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler® kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples; for database and single-source samples, please see our GlobalFiler® Express PCR Amplification Kit.

Key Features:

• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler® kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler® kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler® kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect™ and Identifiler® primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) (Applied Biosystems™)

The VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (5 tubes) combines the features of our VeriFiler™ Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer. The VeriFiler Express kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. It enables multiplex amplification of the 22 STR loci included in the GlobalFiler™ Express kit as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select™, Identifiler™, and VeriFiler™ Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems™ instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit & Prep-n- Go™ Buffer, for buccal swabs (Applied Biosystems™)

The AmpFLSTR® Identifiler® Direct PCR Amplification Kit & Prep-n-Go™ Buffer (for Buccal Swabs) combines the features of our AmpFLSTR® Identifiler® Direct PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer (for Buccal Swabs). The Identifiler® Direct kit enables direct PCR amplification of the same 16 loci included in our standard Identifiler® kit, while eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Prep-n-Go™ Buffer (for Buccal Swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key Features:

• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction?purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder, and primer sequences as the standard Identifiler® kit, which:

• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

AmpFLSTR™ NGM SElect™ Express Kit (Applied Biosystems™)

The AmpFℓSTR® NGM SElect™ Express Kit enables extremely efficient analysis of single-source samples for laboratories wishing to utilize the European Standard Set Loci (ESSL) together with the highly discriminating SE33 locus. The kit utilizes the same primer sequences as the NGM™ and NGM SElect™ kits, but employs a new master mix optimized to enable high quality direct amplification of swab and treated/untreated paper substrates, as well as rapid PCR cycling (< 1 hr).

Key Features of the AmpFℓSTR® NGM SElect™ Express Kit:

• Supports rapid, direct amplification on treated paper and untreated paper/swab substrates in conjunction with Prep-n-Go™ Buffer
     - Facilitates high throughput & automated processing
     - Minimizes extraction, purification, and quantification costs and helps reduce labor and consumable costs
• Contains a new fast-capable enzyme that allows completion of amplification in ~45 mins
• Maximizes first pass success rate, reducing the need for time-consuming repeat amplifications
• Part of our complete integrated solution for efficient single-source sample processing
• Enables laboratories to go from sample to result typically in only 2 hours!

Data Quality Worth Sharing
The AmpFℓSTR® NGM SElect™ Express Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP) and includes the expanded European Standard Set of Loci (ESSL). The NGM SElect™ Express kit simultaneously amplifies the same 16 loci included in the NGM™ kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel.

Powerful Discrimination
The combination of loci in the NGM SElect™ Express kit generates an extremely high power of discrimination of any AmpFℓSTR® kit (see Table 1).

Maximizes Concordance
The NGM SElect™ Express kit maintains identical primer sequences for the core STR loci common to the SGM Plus®, Identifiler® and SEfiler Plus™ kits, thereby:

• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus™ kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus™ kit.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect™ Express kit has been designed with ease of implementation and use in mind. This is a single kit solution that is designed to deliver high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs. The kit workflow is very similar to other AmpFℓSTR® kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened to less than one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

Quantifiler™ Duo DNA Quantification Kit (Applied Biosystems™)

The Quantifiler® Duo DNA Quantification Kit enables forensic laboratories to simultaneously obtain a quantitative and qualitative assessment of total human and human male DNA in a single, highly sensitive real-time PCR reaction. This guides selection of the optimal STR chemistry (autosomal, Y-STR or miniSTR) and streamlines the workflow while increasing downstream analysis success rates.

• Highly Accurate: determines the ratio of human male to female DNA, even with minute male and excess female DNA (1:1000 or greater).
• Highly Informative:
•Guides selection of the optimal AmpFLSTR® PCR Amplification Kit (autosomal, Y-STR or miniSTR),
•Improves prediction of STR amplification performance and estimation of DNA quantity in degraded samples,
•Detects the presence of PCR inhibitors (suggesting additional purification of extract and⁄or MiniFiler™ kit analysis may be necessary).
• Highly Sensitive: 6 pg⁄µl limit of detection.
• Highly Specific: for human and higher primate DNA.
• Highly Efficient: dual quantification system minimizes sample consumption and streamlines workflow.
• Highly Reliable: uses the gold standard TaqMan® real-time PCR technology already widely adopted in forensic laboratories.
• Highly Integrated: optimized and validated as part of a complete DNA testing system with the Applied Biosystems® 7500 Real-Time PCR System and AmpFLSTR® PCR Amplification Kits to provide the highest quality STR profiling results.

A Highly Informative Tool
The Quantifiler® Duo kit has been optimized and validated as part of a complete DNA testing system with the AmpFLSTR® PCR Amplification Kits.
This enables it to function as a critical component of an improved and integrated workflow for more effective processing of forensic casework samples. The workflow diagram in Figure 1 illustrates how the kit functions as a “sample assessment system" which can efficiently detect the presence of inhibitors, determine the ratio of male to female DNA, and guide selection of the optimal AmpFLSTR® PCR Amplification Kit (autosomal, Y-STR or miniSTR) to maximize recovery of interpretable STR profiles.

Kit Configuration
This kit uses increased amplicon sizes for improved prediction of STR amplification performance and estimation of DNA quantity in degraded samples. The amplicon sizes are similar for all three targets to avoid preferential PCR. The kit uses single copy targets for superior accuracy and consistency in quantification results (multi-copy targets are affected by variability between individuals and decrease correlation with the single copy targets in STR kits).

Comparison to Quantifiler® Human and Y Kits
There are many specific differences with the current Quantifiler® kits including:

• The Quantifiler® Duo kit uses an alternative human DNA target (RPPH1) and increased amplicon sizes to improve prediction of STR amplification performance and estimation of DNA quantity in degraded samples.
• The Quantifiler® Duo DNA Standard is pooled human male genomic DNA (rather than cell-line DNA) for improved stability and correlation with forensic samples.
• The Quantifiler® Duo PCR Reaction Mix has been optimized for expanded multiplex performance.
• The Quantifiler® Duo kit has been designed to guide selection of appropriate AmpFLSTR® Kit chemistry and maximize recovery of interpretable STR profiles.

NGM Detect™ PCR Amplification Kit (Applied Biosystems™)

The Applied Biosystems NGM Detect PCR Amplification Kit is the first 6-dye STR kit designed specifically for those laboratories using the European Standard Set (ESS) of loci. The kit offers excellent sensitivity and provides an alternate marker configuration to the well-established NGM SElect PCR Amplification Kit format to maximize information recovery, even from degraded casework samples.

As with all Applied Biosystems STR kits, the NGM Detect kit is ISO18385 Forensic DNA Grade, giving you piece of mind when choosing this kit for your casework samples.

The key features of NGM Detect kit are:
Increased database searches by virtue of prioritization of markers with the highest representation in historic databases
Optimized positioning of SE33 in the sub-350 bp range to support mixture analysis
Dual amplification compatibility when partnered with the NGM SElect kit
High sensitivity through a new master mix formulation coupled with the ability to add up to 15 μL of sample to the reaction
Fast PCR cycle time—<60 min
Additional Y marker enabling gender confirmation
Integrated Quality Control System to verify reaction set up and assess the presence of inhibition in the sample

Increased database searches
The European Standard Set (ESS) of loci was established to enable cross-border information sharing and was composed of seven SGM/SEFiler Plus markers. The ESS was expanded to 12 loci in 2009 to include more markers to enable wider compatibility between neighboring countries.

Because of this, a large number of historic crime-scene profiles contained in databases consist of SGM/SEFiler Plus marker data. When dealing with challenging casework, such as cases involving degraded samples, the information recovery is limited. In this situation, recovering the SGM/SEFiler Plus markers is key for producing a partial DNA profile that has maximum overlap with older data contained in databases, giving a higher probability of a match and providing valuable investigative leads.

The NGM Detect kit takes full advantage of the space afforded by 6 dyes and features optimized marker positioning, placing the SGM/SEFiler Plus markers in the low molecular weight (LMW) range to help ensure the highest compatibility with historic datasets and the possibility of a higher chance of generating investigative leads.

Optimized positioning of SE33
The highly polymorphic SE33 marker is useful for mixture analysis but extends up to 500 bp in most STR kits. As a result, it is one of the first markers to drop out in degraded samples. In the NGM Detect kit, SE33 extends to a maximum of 351 bp to help to significantly increase the chances of recovery of this highly informative marker in challenging samples.

Dual amplification compatibility
Dual amplification, where two different STR kits are used to amplify a sample, is a strategy that can be used to confirm or extend a DNA profile result for increased confidence in the final result in the former and to obtain additional information in the latter.The NGM Detect kit is an advanced partner for the NGM SElect kit, featuring the same loci, but with different primer sequences and different marker placement. When using the two kits in combination, different primer sequences provide confidence that the consensus profile generated reflects the ‘true’ genotype of the sample. The complimentary marker positioning places mini-STR loci in the NGM Detect kit in the high molecular weight (HMW) range in the NGM Select kit and vice versa, meaning that additional alleles can be recovered from degraded samples when using the two kits in combination. Together the NGM Detect and NGM Select system delivers 12 independent mini-STRs.

Integrated Quality Control System
The IQC assay consists of two synthetic sequences not found in nature with primers specific for each of the targets. It provides positive confirmation that the assay is functioning as expected in a given run.

This system is particularly useful to confirm the validity of negative results and can also be used to distinguish samples that are degraded versus those that contain PCR inhibitors, supplementing data analysis and informing downstream processing decisions.

Experience matters
The NGM Detect kit is part of a fully integrated and validated forensic DNA workflow backed by superior global training, service, and support. Technical support scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging 8 years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit (Applied Biosystems™)

Optimized for casework, the AmpFLSTR® Identifiler® Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler® Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

Features of the AmpFLSTR® Identifiler® Plus PCR Amplification Kit include:

• Amplifies the same loci as the Identifiler® kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler® kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler™ Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® and SGM Plus® kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts

Other Improvements and Benefits
The improvements in the Identifiler® Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler® kit. In addition, the Identifiler® Plus Master Mix includes AmpliTaq Gold® DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.

None of the formulation enhancements included in the Identifiler® Plus kit have required modifications to the Identifiler® Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler™ kit has been designed to work in conjunction with the Identifiler® Plus kit, utilizing miniSTR technology to help maximize recovery of information.

The Identifiler® Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler®, Identifiler® Direct, Profiler Plus®, COfiler® or SGM Plus® kits, and facilitate comparison with existing DNA databases.

The Confidence to Do More
The Identifiler® Plus kit is part of a new generation of AmpFLSTR® kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler® Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.

Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs) (Applied Biosystems™)

The GlobalFiler® Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs) combines the features of our GlobalFiler® Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go™ Buffer (for buccal swabs). The GlobalFiler® Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go™ Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key features:
• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput and automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time-to-result with minimal reduction in data quality
• Minimizes extraction and purification costs and helps reduce the cost of labor and consumables

In addition, the GlobalFiler® Express PCR Amplification Kit combines maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency.

The GlobalFiler® Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler® PCR Amplification Kit.

AmpFLSTR™ Identifiler™ PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® Identifiler® PCR Amplification Kit is the single most discriminating and widely used STR based kit for human identification applications from Applied Biosystems®. The Identifiler kit amplifies 15 loci and Amelogenin in a single tube and provides loci consistent with major world-wide STR databasing standards.

Features of the The AmpFLSTR® Identifiler® PCR Amplification Kit:

• Fifteen STR (short tandem repeat) loci and Amelogenin co-amplified in a single tube.
• Loci consistent with all major worldwide STR standards.
• Degenerate unlabeled primer addresses mutations observed in a population of Chamorros and Filipinos from Guam
• Includes 13 core loci required for sample entry in CODIS

Complete Validated Solution
Applied Biosystems offers a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR® STR kits, instrumentation, software, and consumables. The Identifiler kit is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

Five Dye Technology Allows Enhanced Throughput
Identifiler utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET® and LIZ® -dyes were specifically designed to expand the spectral range of the 4-dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.

Experience Matters™
When you partner with us you gain access to the most extensive Human Identification resource network found within the industry. Our dedicated team of industry leading experts includes Research and Development scientists with extensive forensic product development experience, skilled Field Applications Support Scientists with over 100 years of combined forensic experience, and dedicated Field Service Engineers that provide on-site technical assistance with instrumentation.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit (Applied Biosystems™)

The AmpFLSTR® Identifiler® Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler® Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA® Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector™ and other non-FTA® substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems® Prep-n-Go™ Buffer.

Key Features of the AmpFLSTR® Identifiler® Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler® Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler® kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR® kits including the Identifiler®, Identifiler® Plus, Profiler Plus®, Profiler Plus® ID, SGM Plus® and COfiler® Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample typeSuccess Rate
(50 rfu Threshold)
Success Rate
(150 rfu Threshold)
Number of Samples Tested
Blood on FTA™ Card99.4-100%
Mean: 99.5%
95.7-98.8%
Mean: 97.3%
414
Buccal on FTA™ Card91.8-99.4%
Mean: 97.1%
84.2-95.5%
Mean: 90.9%
643
Buccal on Bode Buccal
DNA Collector™ Card
97.5-98.8%
Mean: 97.8%
88.1-98.8%
Mean: 94.0%
582


Table 1: As part of its rigorous development, the Identifiler® Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA® Cards and Buccal DNA Collectors™ were amplified with the Identifiler® Direct Kit, and the resulting data was analyzed using GeneMapper® ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler® Direct Kit is part of a new generation of AmpFLSTR® Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler® Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR® STR Kits, instrumentation, software, and consumables.

The Identifiler® Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems® instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler® Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler® Direct Kit should perform its own internal validation studies.

The Identifiler® Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems® Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.