Shop All Microbial Detection & Identification Kits

TaqMan™ Escherichia coli spp Assay Beads

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs.

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom TaqMan™ Gluconacetobacter liquefaciens Assay Beads

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs.

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.

LSI VetMAX™ Bluetongue Virus Real-Time PCR Kit, BTV1 typing - IAH

Same as Bluetongue Virus BTV1 Typing - IAH Real-Time PCR Kit (BTV1G-IAH)

TaqMan® probes - RNA - duplex - One well reaction

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits).
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009

World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11

Custom TaqMan™ Bacteriodes spp Assay Beads

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs. The assay design is based on US EPA published guidelines and validated by the EPA.

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

Custom assays have been bioinformatically developed and tested for performance. Validation services for specific samples types are provided by our Custom Solutions team. Please contact your local Thermo Fisher Scientific representative for more information.

VetMAX™ BTV4 IAH Typing Kit

Note: The name of this product has changed. It was previously called LSI VetMAX Bluetongue Virus BTV4 Typing - IAH.

Same as TaqVet Bluetongue Virus BTV4 Typing - IAH Real-Time PCR Kit (BTV4G-IAH)

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits)
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

1. U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.

2. World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf.

TaqMan™ EAE Assay, ISO

This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on ISO technical specification TS13136. The assay has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.

Features of TaqMan® assays:

• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use, single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate, reproducible, and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

TaqMan™ Francisella tularensis Detection Kit (Applied Biosystems™)

Applied Biosystems® TaqMan® Francisella tularensis Detection Kit uses the polymerase chain reaction (PCR) to amplify a unique microorganism specific DNA target sequence and a TaqMan® probe to detect the amplified sequence. The TaqMan® Francisella tularensis Detection Kit provides a simple, reliable, and rapid procedure that utilizes the polymerase chain reaction (PCR) to amplify a target sequence unique to Francisella tularensis
• Reduce sample handling with a 3-step process that uses a single, closed-tube format during the PCR step.
• Highly sensitive TaqMan® Real-Time PCR system enables a dramatic reduction in time required for pre-enrichment compared to conventional techniques.
• Streamline your workflow with one standard protocol for multiple organisms.
3 Easy Steps
This kit has 3 easy steps: enrich samples, prepare samples, and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the enrichment step. In the sample preparation step isolate the DNA for PCR, and prepare, mix and pipet the reactions. In the final step, analyze your sample by running a plate on one of our real-time PCR systems.

A Complete Solution
We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems, the TaqMan® Francisella tularensis Detection Kit offers a fully integrated, single-vendor solution to identify Francisella tularensis

TaqMan™ Enterococcus spp Assay Beads

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs. The assay design is based on US EPA published guidelines and validated by the EPA.

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

TaqMan™ STEC O45 & O121 Beads, MLG

These TaqMan® assay beads provide a simple, reliable, and rapid method for detecting the presence of a specific bacterial or viral target. The assay uses PCR to amplify a DNA target unique to the microorganism and TaqMan® probe to detect and quantify the target, all in an an easy-to-use, lyophilized bead format. TaqMan® assay beads deliver the fastest RT-PCR run times on the market (<40 minutes in Fast mode). Simply add 30 µL of purified DNA to the beads and run on Fast or standard instrumentation. These TaqMan® assay beads provide microbial detection capabilities and high-confidence results to help accelerate your food and environmental testing needs. The assay design is based on "Detection and Isolation of non-O157 Shiga Toxin-Producing Escherichia coli (STEC) from Meat Products", method #5B.03 from the Food Safety and Inspection Service (FSIS) Molecular Laboratory Guidebook (MLG).

Features of TaqMan® assay beads:

• Convenience—simply add your nucleic acid sample to the bead-containing assay tube and run; no pre-mixing or master mix preparation required
• Consistency—fewer operator steps means reduced error and increased reproducibility across time, users, and laboratories
• Improved data accuracy—an internal positive control is included to monitor your PCR
• Fast chemistry—40-min run time with Fast cycling, compatible with standard or Fast modes

TaqMan® assay beads are suitable for single or multiplex analysis. Because they are run using universal conditions, multiple bead-based assays can be analyzed in the same run. The robust assay design provides highly accurate, reproducible, and reliable results, with the enhanced stability of a lyophilized format. A passive internal reference based on proprietary ROX™ dye is included for precise data analysis.

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

ViaGram™ Red+ Bacterial Gram Stain and Viability Kit (Invitrogen™)

The ViaGram™ Red+ Bacterial Gram Stain and Viability Kit provides a simple fluorescent staining protocol for fluorescence microscopy that differentially stains many gram-positive and gram-negative bacterial species and, at the same time, discriminates live from dead cells on the basis of plasma membrane integrity.

VetMAX™ BTV8 IAH Typing Kit

Note: The name of this product has changed. It was previously called LSI VetMAX Bluetongue Virus BTV8 Typing - IAH.

Same as TaqVet Bluetongue Virus BTV8 Typing - IAH Real-Time PCR Kit (BTV8G-IAH)

Real-time PCR enables sensitive and specific detection of pathogen nucleic acid in animal samples, allowing for reliable and rapid screening and detection of infected animals.

The LSI VetMAX™ kits include:

• 1 ready-to-use mix that includes a set of oligonucleotides for detection of the target gene OR these separate components that will need to be mixed together: a set of oligonucleotides for the detection of the target gene + a TaqMan® real-time PCR master mix + an RT enzyme (only for RNA kits)
• 1 external positive control of the same nature as the target (inactivated bacteria or virus). Use as a quality control for PCR and/or extraction yield.
• 1 internal positive control (exogenous) to be added at the time of extraction. Use as a quality control for extraction yield.
• A quality control certificate

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

1. U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009; found at: www.cdc.gov/biosafety/publications/bmbl5/BMBL.pdf.

2. World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11; found at: www.who.int/csr/resources/publications/biosafety/Biosafety7.pdf.

TaqMan™ STX1 Assay, ISO

This Taqman® assay provides a simple, reliable, and rapid procedure for detecting the presence of a specific bacterial or viral target. PCR is used to amplify a DNA target unique to the microorganism, then TaqMan® probe is used to detect and quantify the target in a easy-to-use, single tube format. This assay enables microbial detection capabilities with high-confidence results to will help accelerate your food and environmental testing needs. The assay design is based on ISO technical specification TS13136. The assay has been optimized for use with the included Environmental Master Mix; a negative control is not included with this assay.

Features of TaqMan® assays:

• Include an internal positive control to monitor your PCR
• Suitable for single or multiplexed assays
• Easy-to-use, single tube format
• Run using universal conditions- analyze multiple assays in the same run
• Robust assay design provides highly accurate, reproducible, and reliable results
• Include passive internal reference based on proprietary ROX™ dye for precise data analysis

This product is for environmental testing, quality control/quality assurance testing, food and agricultural testing, and research purposes only.

TaqMan™ Cronobacter sakazakii Detection Kit (Applied Biosystems™)

The TaqMan® Cronobacter sakazakii Detection Kit provides a simple, reliable, and rapid procedure for detecting the presence of Cronobacter sakazakii. The assay utilizes the polymerase chain reaction (PCR) to amplify a target unique to that microorganism and TaqMan® probes that identify the specific organism.

• Highly selective identification of C. sakazakii in a wide variety of samples.
• Verified performance
• Ready-to-use convenience
• Minimal risk of contamination

3 Easy Steps
This kit has 3 easy steps: Enrich samples, prepare samples, and run samples. Grow the specific pathogen from a selected sample using a standard enrichment procedure in the Enrichment step. In the Sample Preparation step isolate the DNA for PCR, and prepare, mix and pipet the reactions. The final step is Running a Plate on an Applied Biosystems® SDS or Real-Time PCR System to analyze your sample.

A Complete Solution
We offer a full line of TaqMan® Pathogen Detection kits based on universal assay conditions, enabling you to run any combination of these assays together in the same sample run. In combination with our real-time PCR systems, the TaqMan® Cronobacter sakazakii Detection Kit offers a fully integrated, single-vendor solution to identify Cronobacter sakazakii.

For Research Use Only. Not for use in diagnostics procedures.

PrioCHECK™ Ruminant MAP Ab Serum Plate Kit

Note: The name of this product has changed. It was previously called LSIVet ruminant Paratuberculosis Advanced - Serum.

Single-well indirect ELISA kits enable sensitive and specific detection of pathogen-specific antibodies in animal samples, allowing for reliable and rapid screening and detection of seropositive animals. This kit is for purchase by customers in Germany. Customers outside of Germany should purchase Cat. No. VETPTRS5.

How the kit works:
1. Samples are added to the specific antigen-coated microplate. If present in the sample, specific anti-pathogen antibodies bind to the antigen and form antigen-antibody complexes.
2. After washing, an anti-species peroxidase-labeled conjugate is added, which binds to any antibodies attached to the plate.
3. After a second washing, a chromogenic substrate is added. Color development results from oxidation of the substrate by the conjugated peroxidase.
4. After stopping the reaction, results are read using an ELISA plate reader. Positive samples will show color. The amount of color in each well is proportional to the amount of specific antibodies in the sample.

MicroSEQ™ 500 16S rDNA Sequencing Kit (Applied Biosystems™)

The MicroSEQ® 500 16S rDNA Sequencing Kit is the sequencing component of the MicroSEQ® 500 16S rDNA Bacterial Identification System, which provides an easy-to-use DNA sequence-based method to identify most bacteria. Use this kit to sequence PCR products that have been generated using the MicroSEQ® 500 16S rDNA PCR Kit. The two kits together permit the quick and convenient amplification and sequencing of 500 base pairs of the 16S rRNA gene. Sequences can then be compared to the MicroSEQ® 16S rDNA 500 Library to generate an identity match. This kit does not include a protocol or a Quick Reference Card.

• Sequence-based measurement ensures you get reproducible results in under 24 hours.
• Growth requirement-independent measurement enables you to identify bacteria using objective criteria.
• Universal domain-level primers eliminate up-front optimization.
• MicroSEQ® ID Analysis Software ensures accurate species identification.
• Customizable libraries give you the flexibility to create new libraries and search validated libraries that contain the most frequently encountered organisms.
• Requires minimal training-same work flow as the MicroSEQ® D2 LSU fungal kit.

The MicroSEQ® ID System: A Complete, Integrated Solution
Streamlining every step in microbial identification, Applied Biosystems® MicroSEQ® System combines the advantages of MicroSEQ® ID Software with easy PrepMan Ultra sample preparation reagents and protocols, MicroSeq bacterial and fungal application kits, and industry-leading thermal cyclers and sequencing systems. To ensure optimal performance and system-wide integration, we develop and test all components of the system together.

For Research Use Only. Not for use in diagnostics procedures.