Shop All Forensic DNA Identification Kits

VeriFiler™ Plus PCR Amplification Kit Applied Biosystems™

The Applied Biosystems VeriFiler Plus PCR Amplification Kit is a 6-dye, 25-locus STR kit that amplifies all of the loci present in the Chinese National autosomal DNA database, as well as all required CODIS markers. The VeriFiler Plus kit was specifically developed to maximize sensitivity to enable maximum information recovery from challenging casework samples including touch, degraded, and inhibited samples. The VeriFiler Plus kit includes all of the same markers as the VeriFiler Express and Huaxia Platinum kits, including the highly discriminatory Penta D and Penta E loci. The VeriFiler Plus kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. As with all Applied Biosystems STR kits, the VeriFiler Plus kit is ISO18385 Forensic DNA Grade, giving you piece of mind when using this kit for your casework samples.

Key features of the VeriFiler Plus PCR Amplification Kit include:
• Amplification of all Chinese National database loci and CODIS-required markers
• Concordance with Huaxia Platinum and VeriFiler Express kits; the VeriFiler Plus kit contains the same loci as these databasing kits
• High sensitivity enabled by a high-volume sample load (up to 17.5 µL)
• 11 mini-STRs maximize information recovery from degraded DNA samples
• Internal Quality Control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation, providing actionable information regarding further sample processing or testing
• Very high discrimination power provided by the 25 loci
• Gender confirmation through two Y-chromosome markers
• Efficient workflow with 76-minute PCR cycle time

Casework kit with loci matching DNA profiles in databases
The VeriFiler Plus PCR Amplification Kit was developed to offer the highest level of compatibility between DNA databases and the casework samples genotyped with this kit. Specifically, the VeriFiler Plus kit amplifies all of the loci that are included in the DNA profiles used to build important national and local databases, including the 20 core loci proposed by the Chinese Ministry of Public Security. All required CODIS markers and the highly discriminatory Penta D and Penta E markers are included as well. With 25 markers, the VeriFiler Plus kit offers extremely high discrimination power, enabling matching of casework samples with database profiles with the highest level of confidence.

Exceptional sensitivity enables recovery of more alleles
Developed specifically for casework applications, the VeriFiler Plus PCR Amplification Kit provides DNA analysts with the capability to recover more information from casework samples. The higher sample load volume (up to 17.5 µL) and the inclusion of 11 mini-STRs, as defined by amplicons less than 250 base pairs, provide the highest chance of allele detection. As crime laboratories move toward using DNA forensics in volume crimes, detection of profiles or even partial profiles from samples with a low amount of DNA becomes increasingly important. With an optimized buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples, the VeriFiler Plus kit delivers the capability to provide maximum possible information even from the most challenging samples.

IQC system for more informative results and better decision guidance
The VeriFiler Plus PCR Amplification Kit includes an IQC system that consists of two exogenous, synthetic DNA sequences with primers specific for each IQC target that help distinguish DNA sample degradation from amplification reaction inhibition. In addition, the IQC system serves as a positive control for PCR amplification. The IQC system results, together with an assessment of a DNA profile’s electropherogram, provide DNA analysts with confirmation of inhibition or inferred degradation by virtue of a lack of inhibition, providing data needed to make decisions on additional steps that can be taken to extract more information from a sample.

Experience matters
Thermo Fisher Scientific is the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

We also offer a range of Human Identification Professional Services (HPS) to assist with bringing new kits and workflows online. Since 2007, the HPS team has completed over 400 successful validation projects worldwide with a staff of more than 20 technical support specialists, each averaging eight years of real-world forensic experience, providing customers with in-depth training and support on our instruments, chemistries, and software.

Applied Biosystems™ RapidHIT™ ID ACE GlobalFiler™ Express 50 Sample Kit Applied Biosystems™

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

The Applied Biosystems RapidHIT ID ACE GlobalFiler Express 50 Sample Kit provides 50 self-contained, easy-to-use sample cartridges that contain all necessary reagents for sample preparation, including GlobalFiler Express chemisty, for sample preparation. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and in 90 minutes receive the lab-quality forensic DNA profile.

Features of the kit include:
• 1-minute hands-on-time workflow provided by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 50 RapidHIT ID ACE GlobalFiler Express sample cartridges
• 2 positive control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 2 negative control RapidHIT ID ACE GlobalFiler Express sample cartridges

Applied Biosystems™ RapidHIT™ ID Primary Cartridge GlobalFiler™ Express 100 Kit Applied Biosystems™

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

The Applied Biosystems RapidHIT ID Primary Cartridge GlobalFiler Express 100 Kit provides a reagent cartridge that contains the necessary reagents and consumables for capillary electrophoresis for 100 runs. This primary cartridge also enables easy consumables information tracking so users always know how many runs they have remaining.

This kit includes:
• 1 primary cartridge - GlobalFiler Express 100
• 1 gel cartridge
• 1 utility cartridge
• 1 ACE GlobalFiler Express control cartridge (for install)
• 1 positive control RapidHIT ID ACE GlobalFiler Express sample cartridge (for install)

GlobalFiler™ Express PCR Amplification Kit Applied Biosystems™

The GlobalFiler Express PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.

Key Features:

• Up to 5-times faster amplification time than previous-generation kits
• DNA results in <2 hours—using current CE platforms
• Optimized protocols for blood and buccal samples deposited on swab or paper substrates
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler Express kit is a robust and reliable single-amplification STR multiplex kit optimized for use with database and single-source samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases and utilizes optimized reagents, thermal cycling parameters, and the latest advances in oligo synthesis and purification to enable the highest quality results specific to the sample type being processed. Maintenance of the NGM SElect and Identifiler primer sequences helps to ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

Applied Biosystems™ RapidHIT™ ID ACE GlobalFiler™ Express 150 Sample Complete Kit Applied Biosystems™

NOTE: For use with the Applied Biosystems-branded RapidHIT ID System only (Cat. No. A41810).

The Applied Biosystems RapidHIT ID ACE GlobalFiler Express 150 Sample Complete Kit provides 150 self-contained, easy-to-use sample cartridges and a primary cartridge that contains all necessary reagents for sample preparation (including GlobalFiler Express chemistry) and capillary electrophoresis for 150 runs. With less than one minute of hands-on time, users simply insert the sample (via swab) into the sample cartridge, insert the sample cartridge into the instrument, and in 90 minutes receive the lab-quality forensic DNA profile.

Features of the kit include:
• 1-minute hands-on-time workflow enabled by self-contained, single-use sample cartridges
• Easy consumables information tracking and auto-selection of instrument run parameters through integrated radio frequency identification (RFID) tagging in cartridge

The kit includes:
• 150 RapidHIT ID ACE GlobalFiler Express sample cartridges
• 6 positive control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 6 negative control RapidHIT ID ACE GlobalFiler Express sample cartridges
• 1 primary cartridge - GlobalFiler Express 150
• 1 gel cartridge
• 1 utility cartridge
• 1 ACE GlobalFiler Express Control Cartridge (for install)
• 1 positive control RapidHIT ID ACE GlobalFiler Express sample cartridge (for install)

AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit Applied Biosystems™

Optimized for casework, the AmpFLSTR Identifiler Plus PCR Amplification Kit enables forensic analysts to recover more interpretable results from a wider range of DNA evidence samples with increased confidence. The new kit includes the same primers and allelic ladder as the widely used Identifiler Kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance on mixtures, and the ability to overcome high levels of PCR inhibition.

Features of the AmpFLSTR Identifiler Plus PCR Amplification Kit include:

• Amplifies the same loci as the Identifiler kit with enhanced performance, data quality, and efficiency for casework samples
• Helps significantly improve performance on severely inhibited casework samples through the use of a high performance PCR buffer system and optimized thermal cycling parameters
• Yields higher peak heights from low-level samples and minor contributors in mixtures to facilitate interpretation
• Enables additional analytical flexibility by utilizing fully-validated 28- and 29-cycle protocols
• Streamlines workflow by enabling reduced amplification time
• Maintains the small amplicon size range of the Identifiler kit (<360 bp) for recovery of information from degraded samples; works in conjunction with the MiniFiler Kit to help maximize recovery from heavily degraded samples
• Maintains primer sequences for genotyping concordance with samples typed with the Identifiler, Identifiler Direct, Profiler Plus, COfiler and SGM Plus kits
• Utilizes an improved primer manufacturing process to help significantly reduce artifacts

Other Improvements and Benefits
The improvements in the Identifiler Plus kit formulation allow for more rapid amplification, eliminating approximately 1 hour from the amplification step compared to the Identifiler kit. In addition, the Identifiler Plus Master Mix includes AmpliTaq Gold DNA Polymerase, which eliminates 2 tubes and an extra box from the kit packaging, and enables streamlined reaction setup.

None of the formulation enhancements included in the Identifiler Plus kit have required modifications to the Identifiler Kit’s allelic ladder or 5-dye configuration. This configuration allows for optimal locus spacing and amplicon size ranges no greater than 360 bp, enabling better data recovery from degraded samples as compared to other multiplex systems with significantly larger amplicon size ranges. For heavily degraded samples, the MiniFiler kit has been designed to work in conjunction with the Identifiler Plus kit, utilizing miniSTR technology to help maximize recovery of information.

The Identifiler Plus kit also maintains primer sequences to enable genotyping concordance for samples previously typed using the Identifiler, Identifiler Direct, Profiler Plus, COfiler or SGM Plus kits, and facilitate comparison with existing DNA databases.

The Confidence to Do More
The Identifiler Plus kit is part of a new generation of AmpFLSTR kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Plus kit could significantly increase their ability to process difficult and challenging casework samples, and expand the types of cases from which probative results can be generated.

Experience Matters
We are the only company that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service scientists and Field Application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. Our team of forensic experts is also available to provide in-depth training and can support the planning and efficient execution of internal validation projects in your laboratory. All of these resources help ensure success when using our human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

AmpFLSTR™ Identifiler™ Direct PCR Amplification Kit Applied Biosystems™

The AmpFLSTR Identifiler Direct PCR Amplification Kit enables direct amplification of the 16 loci included in the Identifiler Kit, eliminating the need to perform DNA extraction or purification steps for DNA database and other single source reference samples. Blood or buccal samples on FTA Cards can be punched into PCR plates or tubes, and taken directly to PCR amplification, without reduction in data quality. Samples collected with the Buccal DNA Collector and other non-FTA substrates can be processed without the need for heat incubation or additional workflow steps using the Applied Biosystems Prep-n-Go Buffer.

Key Features of the AmpFLSTR Identifiler Direct PCR Amplification Kit:

• Enables the direct amplification of blood and buccal samples without the need for extraction or purification
• Facilitates high throughput & automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time to result with minimal reduction in data quality
• Minimizes extraction/purification costs and helps reduce the cost of labor and consumables

In addition, the Identifiler Direct kit uses the same dye set, allelic ladder and primer sequences as the standard Identifiler kit, which:
• Maximizes concordance with STR profiles generated with other AmpFLSTR kits including the Identifiler, Identifiler Plus, Profiler Plus, Profiler Plus ID, SGM Plus and COfiler Kits
• Facilitates comparison with casework data and legacy databases
• Streamlines validation and implementation

Sample typeSuccess Rate
(50 rfu Threshold)
Success Rate
(150 rfu Threshold)
Number of Samples Tested
Blood on FTA Card99.4-100%
Mean: 99.5%
95.7-98.8%
Mean: 97.3%
414
Buccal on FTA Card91.8-99.4%
Mean: 97.1%
84.2-95.5%
Mean: 90.9%
643
Buccal on Bode Buccal
DNA Collector Card
97.5-98.8%
Mean: 97.8%
88.1-98.8%
Mean: 94.0%
582


Table 1: As part of its rigorous development, the Identifiler Direct Kit was field tested by several medium to high-throughput DNA databasing laboratories to assess "first pass" success rate. Combined results from external test site laboratories are shown below. 1.2 mm punches from blood and buccal samples on FTA Cards and Buccal DNA Collectors were amplified with the Identifiler Direct Kit, and the resulting data was analyzed using GeneMapper ID-X, at two different minimum peak amplitude thresholds.

The Confidence to Do More
The Identifiler Direct Kit is part of a new generation of AmpFLSTR Kits that harnesses significant advances in PCR amplification technology to enable recovery of more results from more samples with increased confidence.

Ultimately, laboratories using the Identifiler Direct Kit will be able to significantly expand their throughput capabilities, while also maximizing sample integrity and quality assurance for the entire process.

Complete Validated Solution
We offer a complete solution to meet the needs of the human identification community. Our system includes DNA extraction and quantification kits, a range of AmpFLSTR STR Kits, instrumentation, software, and consumables.

The Identifiler Direct Kit is part of a comprehensively validated system for STR-based Human Identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. Validation experiments for the Identifiler Direct Kit were performed by our scientists according to the DNA Advisory Board (DAB) Quality Assurance Standards for Forensic DNA Testing Laboratories and the Scientific Working Group on DNA Analysis Methods (SWGDAM) Revised Validation Guidelines. However, each laboratory using the Identifiler Direct Kit should perform its own internal validation studies.

The Identifiler Direct Kit is subjected to rigorous quality control testing to ensure consistent performance. We develop and manufacture our products in accordance with ISO 9001 quality system requirements. Additionally, a Certificate of Analysis is available upon request that confirms that the specific combination of components within each lot meets quality assurance testing specifications.

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical Service Scientists and Field Application Specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems Human Identification solutions in your laboratory.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

GlobalFiler™ PCR Amplification Kit Applied Biosystems™

The GlobalFiler PCR Amplification Kit is the first 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards, with dramatically reduced amplification time and superior discrimination power, helping to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency. The GlobalFiler kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples and is also available in a format containing an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. For database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

Key Features:

• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples
• The only kit with all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility

The GlobalFiler kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages new dyes for the incorporation of additional markers, generating the most discriminating kit available to the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, designed to deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The GlobalFiler kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM SElect and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, multiple gender markers have been added to the multiplex to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience Matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ Express PCR Amplification Kit and Prep-n-Go™ Buffer (for buccal swabs) Applied Biosystems™

The GlobalFiler Express PCR Amplification Kit and Prep-n-Go Buffer (for buccal swabs) combines the features of our GlobalFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer (for buccal swabs). The GlobalFiler Express kit is the first 6-dye, 24-locus STR kit that enables direct PCR amplification, eliminating the need to perform DNA extraction or purification steps for DNA database and other single-source reference samples. Prep-n-Go Buffer (for buccal swabs) is specifically designed to enable high-quality direct PCR amplification of single source samples collected on untreated paper or buccal swabs.

Key features:
• Enables the direct amplification of buccal swab samples without the need for extraction or purification
• Facilitates high throughput and automated sample processing
• Minimizes the risk of contamination and procedural errors by reducing the number of processing steps
• Helps accelerate time-to-result with minimal reduction in data quality
• Minimizes extraction and purification costs and helps reduce the cost of labor and consumables

In addition, the GlobalFiler Express PCR Amplification Kit combines maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, to enable forensic DNA labs worldwide to maximize information recovery and improve overall efficiency.

The GlobalFiler Express kit is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for database and single-source samples; for casework samples, please see our GlobalFiler PCR Amplification Kit.

AmpFLSTR™ MiniFiler™ PCR Amplification Kit Applied Biosystems™

The AmpFLSTR MiniFiler PCR Amplification Kit increases your ability to obtain DNA results from compromised samples that previously would have yielded limited or no genetic data. This means cold cases can come off the shelf for re-analysis and new, challenging samples have a better chance of delivering interpretable results. This is the world's first commercially available miniSTR kit.

Features of the AmpFLSTR MiniFiler PCR Amplification Kit:

• A single amplification provides information on the eight largest and most difficult to amplify loci in the AmpFLSTR Identifiler and SGM Plus.
• With amplicon sizes less than 270 bp, MiniFiler is optimized for use with the most difficult types of samples including those that are degraded and contain inhibitors.
• Ability to overcome the inhibitory effects of substances such as heme and humic acid which are commonly encountered in forensic samples.
• Can be used as an adjunct to other commercially available amplification kits to recover the larger loci, which cannot be amplified or that drop out.
• Utilizes proven 5-dye technology allowing compatibility of workflows with Identifiler.

Five Dye Technology Allows Enhanced Throughput
MiniFiler utilizes the most advanced 5-dye technology to allow for the multiplex of more loci in a single lane or injection helping to increase your throughput and to simplify the workflow needed to obtain probative results. Our PET and LIZ dyes were specifically designed to expand the spectral range of the 4 dye system. We have expanded the spectral range to 660 nm allowing for maximum color separation with minimal spectral overlap. As with all of our products, the five-dye technology is validated for use with our latest instruments and software as a complete system.

Experience Matters
When you partner with us you gain access to the most extensive knowledge and experience of our forensic support team and training resources dedicated to human identification laboratories. Our HID University provides continuing education for DNA professional, our support and Applications Scientist offer over 100 years combined forensic experience, and our extensive network of Field Service Engineers provide technical assistance with instrumentation.

Complete Validated Solution
We offer a complete solution for your human identification needs. Our system includes DNA quantification kits, a range of AmpFLSTR STR kits, instrumentation, and consumables. Minifiler is part of the most comprehensively validated systems for STR-based Human Identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations including: SWGDAM, DAB, CODIS, ENFSI, Interpol, and GITAD.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

AmpFLSTR™ NGM SElect™ PCR Amplification Kit Applied Biosystems™

The NGM SElect Kit is a highly robust, single amplification Short Tandem Repeat (STR) multiplex kit developed for human identification laboratories wishing to utilize the expanded European Standard Set of Loci along with the highly polymorphic SE33 locus. The NGM SElect Kit combines the latest advances in primer synthesis, buffer enhancement and thermal cycling optimization. This enables the kit to deliver high sensitivity and improved STR performance for forensic casework and database samples in one easy workflow.

Key Features of the NGM SElect Kit:

• Exceptional data quality and discrimination capabilities support international data sharing
• Enables significantly enhanced performance on inhibited and degraded samples
• Enables greater sensitivity
• Maximizes concordance with previously typed samples
• Facilitates confident interpretation, promotes an optimized workflow and streamlines the implementation process

Data Quality Worth Sharing
The NGM SElect Kit has been designed to deliver a powerful level of discrimination and data quality to support cross-border data sharing initiatives. The kit addresses the requirements stipulated by the European Network of Forensic Science Institutes (ENFSI) and European DNA Profiling Group (EDNAP), and includes the expanded European Standard Set of Loci (ESSL).

The NGM SElect kit simultaneously amplifies the same 16 loci included in the NGM kit (D3S1358, vWA, D16S539, D2S1338, D8S1179, D21S11, D18S51, D19S433, TH01, FGA, Amelogenin, D10S1248, D22S1045, D2S441, D1S1656, D12S391) with the addition of the highly polymorphic SE33 locus in the PET (red) dye channel (Figure 1).

Configured for Success
Utilizing our expertise in 5-dye and mobility modifier technology, 17 loci are accommodated in a single amplification while maintaining optimum spacing (Figure 2). This permits unambiguous allele designation. All amplicons fall below 450 base pairs (bp) to maximize performance across a wide range of forensic samples. The majority of loci are concentrated below 200 bp, enabling recovery of a greater number of alleles from challenging degraded samples.

Powerful Discrimination
The combination of loci in the NGM SElect kit generates the highest power of discrimination of any AmpFLSTR kit (Table 1). This helps minimize adventitious matches when comparing large data sets (e.g. for cross border data exchange). The NGM SElect kit also offers utility for other applications requiring higher levels of discrimination, including:
• Complex kinship analysis
• Disaster victim identification
• Missing person investigations

Permits Significantly Enhanced Performance on Inhibited and Degraded Samples
Leveraging the recent advances in PCR amplification technology pioneered with the NGM kit, the NGM SElect kit enables recovery of data from severely inhibited and degraded samples. For example, the NGM SElect kit is able to extract full profiles even from samples containing high concentrations of PCR inhibitors such as humic acid (Figure 3).

Performs with Greater Sensitivity
The NGM SElect Kit benefits from the same sensitivity enhancements as the NGM Kit, resulting in superior allele recovery at lower input levels (Figure 4). This can also enhance detection of the minor contributor in a mixed sample. The standard 29 cycle, 1 ng DNA input protocol enables maximum profile balance, and a validated 30 cycle protocol is also available. The higher cycle number leads to a corresponding increase in signal, increasing the possibility of allele detection from lower input DNA amounts. The use of 30 cycles also results in offscale data at an input of 1 ng (Figure 4); therefore, laboratories wishing to adopt this protocol should determine the optimum input DNA appropriate to their workflow through internal validation studies.

Maximizes Concordance
The NGM SElect Kit maintains identical primer sequences for the core STR loci common to the SGM Plus, Identifiler and SEfiler Plus kits, thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration with previous kits to reduce training and implementation requirements

The primers for the SE33 locus were redesigned from those used in the SEfiler Plus kit to relocate the amplicon within the multiplex. However, the new primer sequences have been optimized and tested thoroughly to maintain concordance with the SEfiler Plus kit as much as possible.

Facilitates Confident Data Interpretation
The NGM SElect kit benefits from improvements in primer synthesis techniques resulting in significantly cleaner baselines than previous AmpFLSTR kits (Figure 5). The lack of dye related artifacts, even at 30 cycles, improves the ability of the analyst to interpret data, particularly for low level samples. Confident data interpretation is further enhanced by other improvements in profile quality, including:
• Enhanced sensitivity and robustness
• Maximized heterozygote and intracolor balance
• Optimum dye set utilization

These features also help facilitate streamlined processing of single source samples by expert system software such as GeneMapper ID-X.

Easy to Implement, Easy to Use
Every aspect of the NGM SElect kit has been designed with ease of implementation and use in mind. This is a single kit solution that delivers high quality results for both database and casework samples, optimizing the forensic DNA workflow while reducing operating costs.

The kit workflow is very similar to other AmpFLSTR kits, enabling forensic laboratories to streamline validation and implementation. However, the enzyme is now contained within the master mix, simplifying reaction set-up. Cycling times have been shortened by approximately one hour, allowing laboratories to achieve higher quality results more quickly. For compatibility with both manual and automated systems, two package sizes are available, the standard 200 reaction kit and a larger 1000 reaction kit. The larger package includes larger bottles to facilitate use on liquid handling platforms.

Note: For Forensic or Paternity Use Only.

Important Notice

The purchase of this product conveys to the buyer the non-transferable license to use the purchased amount of the product only for forensic or paternity testing conducted by the buyer. This license does not include, as ruled by a U.S. Court, a license to use this product in chimerism determination or analysis, molar specimens classification, cell line authentication, determination of fetal sex, cancer analysis, genetic research, non-casework-related forensic applications (such as general research in forensics or teaching and training persons not employed in a forensic laboratory), identifying or determining maternal cell contamination, or sample tracking. Further rights can be obtained by contacting Promega Corp., 2800 Woods Hollow Rd., Madison, WI 53711 USA.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

VeriFiler™ Express PCR Amplification Kit Applied Biosystems™

The VeriFiler Express PCR Amplification Kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. The VeriFiler Express kit enables multiplex amplification of the 22 STR loci included in the GlobalFiler Express kit, as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select, Identifiler, and VeriFiler Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

Yfiler™ Plus PCR Amplification Kit Applied Biosystems™

Note: The Yfiler Plus kit is now approved for use by laboratories generating DNA profiles for inclusion in the US National DNA Index System (NDIS) CODIS database.

The Yfiler Plus PCR Amplification Kit is designed for forensic laboratories that process highly challenging Y-STR casework and database samples. The Yfiler Plus kit is optimized to help improve resolution of trace evidence and sexual assault mixtures that contain small quantities of male DNA in a large background of female DNA and offers laboratories both the power of discrimination and the performance to enable accurate results in less time from challenging samples.

The Yfiler Plus PCR Amplification Kit is a 6-dye multiplex assay for short tandem repeats (STRs) that allows amplification from multiple male-specific sample types, such as male–male and male–female mixtures, and direct PCR amplification from single-source samples.

Key features of the Yfiler Plus PCR Amplification Kit:
• Improved discrimination capacity through new, highly discriminating markers
• Improved performance with degraded samples and PCR inhibitors
• Improved genotyping accuracy through expanded allelic ladder and virtual bins
• Concordance with existing data in reference haplotype databases
• Increased workflow efficiency enabling faster time-to-results than previous Y-STR kits

Improved discrimination capacity
The Yfiler Plus PCR Amplification Kit enables multiplex amplification of the 17 Y-STR markers included in the AmpFLSTR Yfiler PCR Amplification Kits, as well as 10 new Y-STRs using 6-dye chemistry to offer the highest discriminatory capacity of any commercially available Y-STR kit. In particular, the inclusion of seven rapidly-mutating (RM) Y-STRs with mutation rates above 1 × 10(-2) helps improve resolution of paternal lineage differentiation as well as helps with discrimination of closely-related males.

More information from challenging casework samples
Y-STR analysis is useful for processing of complex casework samples with trace quantities of male DNA in presence of high level of female DNA. These samples may include rape kit samples, body fluid with few or no sperm present, and fingernail scrapings from female victims of violent assaults. These samples often show DNA degradation and contain inhibitors that can make the analysis difficult and lead to inconclusive results. The Yfiler Plus kit harnesses the latest developments in PCR amplification technology to help provide cleaner baseline and improved recovery of alleles with mixtures and highly inhibited samples.

Streamlined protocol and increased workflow efficiency
The Yfiler Plus kit provides faster thermal cycling conditions enabling shorter time to result. The kit is also designed to process single-source reference samples using direct PCR amplification from the following types of samples:
• Blood and buccal samples on treated paper substrates, without the need for sample purification
• Blood samples collected on untreated paper substrates and treated with Prep-n-Go Buffer
• Buccal samples collected on swab substrates and treated with Prep-n-Go Buffer

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The Yfiler Plus kit is part of a comprehensively validated system for STR-based human identification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as SWGDAM and DAB.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

GlobalFiler™ IQC PCR Amplification Kit  Applied Biosystems™

The GlobalFiler IQC PCR Amplification Kit is a 6-dye, 24-locus STR kit that combines maximum compatibility with global databasing loci standards. It provides superior discrimination power and an internal quality control (IQC) system, helping forensic DNA labs worldwide maximize information recovery and improve overall efficiency. The GlobalFiler IQC kit was specifically developed to maintain the performance and core marker set of the GlobalFiler kit while enabling an assessment of sample quality in the STR profile. It is part of a fully integrated and validated forensic DNA workflow backed by best-in-class global training, service, and support. This kit is optimized for casework samples; for database and single-source samples, please see our GlobalFiler Express PCR Amplification Kit.

Features of the GlobalFiler IQC PCR Amplification Kit include:
• Internal quality control (IQC) system enables detection of and discrimination between amplification inhibition and DNA sample degradation and an in-sample positive amplification control, providing actionable information
• Efficient protocols minimize amplification time compared to previous-generation kits
• An optimized buffer system and expanded DNA input volume delivers maximum sensitivity for trace/low level DNA samples
• Enhanced intracolor balance simplifies interpretation of mixture samples
• The inclusion of 10 mini-STRs maximizes results from degraded samples (<220 bp)
• Contains all markers recommended for inclusion by the CODIS Core Loci Working Group, including all markers commonly used in Europe, for maximum global database compatibility
• Improved data interpretation with reduced pull-up edits and off-scale data recovery when combined with 3500 Data Collection Software v4.0 and GeneMapper ID-X Software 1.6
• Manufactured to IS0 18385 standards

The GlobalFiler IQC kit is a robust and reliable single-amplification STR multiplex kit optimized for use with the most challenging casework samples. The kit leverages six dyes for the incorporation of additional markers, generating a highly discriminating kit for use by the forensic community. The kit contains all of the markers included in the major global databases, including 10 mini-STRs, to help deliver optimized results with even highly degraded samples.

Developed specifically for casework applications, the GlobalFiler kit contains a buffer system that delivers greater sensitivity for enhanced performance with inhibited and low-concentration DNA samples. The addition of the IQC provides more insight into sample quality to assist in distinguishing between inhibition and degradation while also providing a positive control for amplification. The GlobalFiler IQC kit utilizes the latest advances in oligo synthesis and purification, which provide a clean baseline and a balanced profile, resulting in simplified data analysis. Maintenance of the NGM Select and Identifiler primer sequences helps ensure maximum concordance with previously typed samples. In addition, the GlobalFiler IQC kit contains multiple gender markers to enable accurate typing of male samples, even highly degraded or Y-negative samples, while increased genotyping accuracy is obtained with the expansion of the allelic ladder allele ranges to include all rare alleles as described in the literature (STRbase).

Experience matters
Thermo Fisher Scientific designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit thermofisher.com/HIDlicensing.

VeriFiler™ Express PCR Amplification Kit with Prep-n-Go™ Buffer (for buccal swabs) Applied Biosystems™

The VeriFiler Express PCR Amplification Kit with Prep-n-Go Buffer (for buccal swabs) combines the features of our VeriFiler Express PCR Amplification Kit with the convenience of sample processing using our Prep-n-Go Buffer. The VeriFiler Express kit is a direct-amplification STR kit that combines a high power of discrimination with superior performance and fast time to results. It enables multiplex amplification of the 22 STR loci included in the GlobalFiler Express kit as well the highly discriminating Penta D and Penta E loci and two gender markers (Amelogenin, Yindel) for maximum concordance with existing kits and high confidence in paternity and relationship testing conclusions.

Powered by breakthrough 6-dye technology, the VeriFiler Express PCR Amplification Kit was designed for optimal performance and excellent discrimination power to help obtain accurate results for single-source samples in less time.

Key features of the VeriFiler Express PCR Amplification Kit:
• Enables direct amplification from these common sample types used in paternity testing without the need for extraction or purification:
    --Buccal samples on treated and untreated paper
    --Buccal samples on cotton swab
    --Blood samples on treated and untreated paper
• Helps accelerate time-to-result with minimal reduction in data quality
• Facilitates high throughput and automated processing through increased workflow efficiency
• Improves genotyping accuracy through superior primer design
• Enhances genotyping accuracy through expanded allelic ladder and virtual bins

High first-pass success rates improve productivity
The VeriFiler Express PCR Amplification Kit enables high-quality direct PCR amplification of reference samples with an improved first-pass success rate. You can expect:
• Well-resolved peaks above the background noise level
• Minimal occurrence of allelic drop-out, off-ladder allele calls, and off-scale data
• Well-balanced profile within each dye color for maximized Intracolor Balance (ICB) values

Maximizes concordance
The VeriFiler Express PCR Amplification primers have been optimized and tested thoroughly to maintain concordance with the GlobalFiler Express, NGM Select, Identifiler, and VeriFiler Direct kits thereby:
• Maximizing concordance with existing data sets for simplified historical comparisons
• Minimizing requirements for repeat analyses necessary to resolve non-concordant events
• Maintaining sufficient similarity of configuration for ease of training and implementation

Complete validated solution
Our products provide a complete solution for your human identification needs and include DNA quantification kits, a range of STR kits, instrumentation, software, and consumables. The VeriFiler Express PCR Amplification Kit is part of a comprehensively validated system for STR-based human identification. The PCR reaction components, primer sequences, and amplification protocols have been developed, optimized, and validated together with Applied Biosystems instrumentation and software to provide specific, robust amplification. All reagents are developed in accordance with guidelines from leading worldwide forensic organizations such as the Scientific Working Group on DNA Analysis Methods (SWGDAM).

Validation experiments for the VeriFiler Express PCR Amplification Kit were performed by our scientists according to the Quality Assurance Standards for DNA Databasing Laboratories and the SWGDAM Validation Guidelines for DNA Analysis Methods. However, each laboratory using the VeriFiler Express PCR Amplification Kit should perform its own internal validation studies.

Commitment to quality
The VeriFiler Express PCR Amplification Kit is subjected to rigorous quality control testing to offer consistent performance. We develop and manufacture our products in accordance with ISO 9001 and ISO 18385 quality system requirements. Additionally, a Certificate of Analysis that confirms that the specific combination of components within each lot meets quality assurance testing specifications is available online.

Experience matters
We are the only company in the world that designs and validates its reagents, instruments, and data analysis software together as an integrated system for DNA testing. Technical service scientists and field application specialists are available to answer your technical questions, review data, and make recommendations on how to perform assays and troubleshoot results. All of these resources help ensure success when using Applied Biosystems human identification solutions in your laboratory.

For licensing and limited use restrictions visit www.thermofisher.com/HIDlicensing.

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